Acarine disease of young honey bees appeared in Pakistan for the first time in epidemic form, inflicting 85% loss of honeybee colonies during 1982-83. Application of the dequeening method for the control of tracheal mite disease gave encouraging results particularly in the queen-right and dequeened colonies. The queen-right and colonies were recovered sooner than the dequeened colonies. A supplementary fumigation of Folbex strip is helpful. The supply of a booster clean package bees to the queen-right colonies helped in building up the colony strength quickly.