Quart for the new antimicrobial agent is still there and the present work is an attempt in this regard, Ethanolic extract of Gliricidia sepium in different concentration was used to investigate its antimicrobial activity against gram +ve and gram-ve bacteria. The study was also extended to some species of fungi. Results showed that the activity was more pronounced against gram +ve organisms and fungi. Maximum inhibition activity was calculated against all the groups of organisms, which was between 0.5 and 1mg ml-1 against bacteria and 2.5 mg ml-1 against fungi. It is therefore concluded that G. sepium provide a lead towards the exploration of new antimicrobial agent.