Chalcones and aurones are compounds having appealing pharmacological profile due to presence of carbonyl system. The bio-screening of 13 compounds was conducted and assessed against S. aureus, C. albicans and E. coli by agar well diffusion method. Chalcones analogues 1, 2, 3, 4, 5, 6, 7, and 8 were found to be inactive or insignificant against E. coli. Chalcone analogue 9 showed moderate activity against E. coli. Similarly, compounds, 1, 2, 3, 6, 7, 8 and 9 were found to be inactive or insignificant against S. aureus. Analogues 4 and 5 displayed moderate bioactivity against S. aureus. Similarly analogues 1, 2, 4, 7, 8 and 9 were found to be inactive or insignificant against C. albicans. Compound 3, 5, and 6 were found more effective against C. albicans. Among aurones 2, 3, and 4 showed insignificant activity while compound 1 showed significant activity against E. coli. Analogue 1 showed very effective acitivity against S. aureus. In a similar way, analogue 3 was found to be inactive, while analogues 1, 2 and 4 showed moderate activity against C. albicans. Overall, chalcone and aurone analogues have been effective against selected bacteria and fungi strains and are expected to be effective in other bioscreening studies.