Wood structure of Masquite (Prosopis julioflora) grown in Punjab was studied and the basic anatomical data was compiled to compare with that of grown in Sindh in order to observe variations and find out difference in quality of wood. Permanent slides of cross, radial and tangential sections were prepared and examined under the microscope for various structural features. Data were collected for the frequency and dimensional measurements of various wood elements / structures in the wood. Results showed that minute variations exist in general in average values of different anatomical features in Masquite wood grown in both areas. In Masquite wood grown in Punjab, the fibers are fairly longer and thick-walled, the wood rays are smaller in size and lower in frequency and the vessels are larger in diameter. The wood may be relatively better in strength, less non-durable and easier to season and preserve than that of grown in Sindh.