Intro: The role of white rot basidiomycetes in the biodegradation of lignocellulosic materials is now well established (Janshekar and Fiechter, 1983; Kirk, 1984). Accordingly, numerous studies have been carried out on the comparative ligninolytic activities of a number of such basidiomycetes when grown on a variety of substrates under different cultural conditions (Golovlev et al., 1983; Levonen-Munoz et al., 1983; Zadrazil and Brunnert, 1980). Biodegradation of lignin in the nature, furthermore, is of particular importance because of its high recalcitrance (Reddy, 1984) and protection of cellulose from attack by various cellulolytic microbes (Fan et al., 1982) thereby preventing bioavailability of as much as half the photosynthethically produced high energy carbon. Though lignocellulosic biodegradation under laboratory conditions has been well investigated, reports on this aspect under natural conditions are limited (Zadrazil et al., 1982). Such studies are necessary not only for an understanding of the degradation process but have the applied potential to optimize production of feed and feed stack from lignocellulosics. In the present paper has been presented biochemical composition of Dalbergia sissoo degraded by various brown and white rot fungi in the natural habitat, their cow reticulo-rumen digestibility, and isolated D. sissoo lignin bio-degradation by some basidiomycetes under cultural conditions.