A study to assess the effect of growth regulator (Gibberellic acid) on seed germination of Colchicum luteum was conducted at PFI, Peshawar. Different lots of seeds were treated against 100, 200, 300 and 400 ppm solution of Gibberellic acid for 24 hours at room temperature and were sown in polythene tubes filled with a mixture of soil, sand and farm yard manure in equal proportion for germination test. It was found that seed lot treated with 300 ppm solution of Gibberellic acid give highest germination percentage.