Total biomass.Percentage of tropane alkaloids.Total alkaloid per plant.Ornithine decarboxylase (ODC) activity, arginine decarboxylase (ADC) activity, arginine were determined separately in roots, stems and leaves of Atropa acuminate fed with nutrient solution having different source of nitrogen fertilizer. All sources of nitrogen fertilizer increased the total biomass and the highest increase (27.2% of control) was in the plants fed with both ammonium and nitrate. The highest increase (34.7% of control) in the alkaloid concentration was in the plants fed with only ammonium.The increase in the Putrescine level. ODC and ADC activities was higher in the plants fed with only ammonium of both ammonium and nitrate as compared to those plants fed with only nitrate.