Aloe vera L. belonging to family Liliaceae is a very important worldwide used plant having nutritional as well as medicinal value. It plays an important role in antimicrobial action. The current study was aimed to assess of antibacterial activity of ethanolic, methanolic and aqueous extracts of Aloe vera L. against six bacterial strains (Staphylococcus aureus; Streptococcus pyogenes; Shigella sonnei; Escherichia coli (1) (4C11); Escherichia coli (2) (ATCC 13048) and Neisseria gonorrhoeae). Agar well diffusion method was applied to assess the antibacterial activity of these extracts. Their antibacterial activities were associated with typical chemical like gentamicine (antibiotic) as positive control. This study revealed that S. pyogenes, S. aureus and N. gonorrhoeae had the maximum inhibition to different extracts of A. vera plant and selected plant extracts remained less effective against Shigella sonnei, E. coli (1) and E. coli (2). The data also revealed that methanolic extract showed relatively more inhibitory effects on bacterial growth as compared to other solvent extracts.