In this study investigation of different carbon and nitrogen sources in various types of media compositions was made to optimize the fermentation and mass production of Pseuodomonas spp. Among all the media compositions, the suitable media containing carbon and nitrogen sources were found as media No.9 (Corn flour 50g/L, Glucose 8g/L, Soybean Cake Powder 20g/L, Wheat bran 20g/L, NaCl 4g/L) followed by No.10 (Corn flour 40g/L, Soybean Cake Powder 30g/L, Yeast Extract 5g/L, Wheat bran 20g/L, NaCl 4g/L, KH2PO4 5/L, MgSO4 1.5g/L), No.08 (Corn flour 17g/L, Glucose 10g/L, Soybean Cake Powder 25g/L, Maltose 10g/L, Yeast Extract 5g/L) and No.05 (Corn flour 5g/L, Yeast Extract 1.5g/L, Fish Peptone 2.5g/L, Soybean Cake Powder 20g/L, Peptone 0.75g/L, KH2PO4 3g/L, CaCO3 0.5g/L, MgSO4 0.3g/L) for the mass production of Pseudomonas spp., after 100 hours (4days). The bacterial culture filtrates produced during fermentation in different media compositions showed varied results on the bioassay against the second stage juvenile mortality (J2) of H. avenae. The culture filtrates formed in media No.8 (Corn flour 17g/L, Glucose 10g/L, Soybean Cake Powder 25g/L, Maltose 10g/L, Yeast Extract 5g/L) and media No.5 (Corn flour 5g/L, Yeast Extract 1.5g/L, Fish Peptone 2.5g/L, Soybean Cake Powder 20g/L, Peptone 0.75g/L, KH2PO4 3g/L, CaCO3 0.5g/L, MgSO4 0.3g/L) has more toxic effects on the second stage juvenile (J2) mortality as compared to rest of secondary metabolites produced in different media and control treatments. All tested strains of Pseudomonas spp. showed stability on pH ranges from acidic pH5 to neutral pH7 and towards basic pH8 against the second stage juvenile (J2) mortality assay.