Bacillus thuringiensis (Bt) is a bacterium that can be used as substitute for various chemical compounds especially insecticides. The insecticidal property of B. thuringiensis comes from the production of Crystal protein. The present study is focused on the cloning and characterization of Cry1AB gene which expresses to produce crystal protein. Cry1AB gene was amplified using specific primers which gave successful PCR results of 3.5 kb long gene amplification. The amplified gene was further verified by inserting it into the expression vector. Furthermore, a computational approach was used to understand evolutionary relationships among Cry1AB gene and to identify conserved protein domains in Cry1AB proteins. Use of insilico approach provided new insights of understanding functional characteristics of Cry1AB gene and its variants.