The parasitic study on catfish Rita rita of river Indus, Jamshoro, Sindh, Pakistan. A total of 22 host catfishes were collected from study area and brought to the Parasitological Laboratory, Department of Zoology University of Sindh Jamshoro. During examination of helminths a total of 28 trematodes were collected from intestine and stomach. The trematodes were resemble with species Aphanururoides lethrini in all diagnostic characteristics and identify as such. Present genus reported first time from Rita rita of Pakistan, hence this report is new locality and host record.