Pollen morphology of two different species, Tragopogon dubius and T. gracilis, belonging to family Asteraceae was studied based on specimens collected within Pakistan. Characters like grain, shape of pollen grain, equatorial view, polar view, equatorial diameter (E), polar diameter (P). P/E ratio, length of colpus, exine surface, exine thickness, inter poral distance, inter spinal distance, inter spinal outline, length of spines, number of spines between colpi in each species were recorded for comparison. At species level, micromorphological differences and distribution of surface pattern, shape and size of pollen have been found to exist. The pollen grains are consistently echinate, trizonocolporate but in Tragopogon dubius these are tetrazonocolporate. Maximum equatorial view, polar view, equatorial diameter (E). polar diameter (P), P/E ratio, length of colpus, exine surface, exine thickness, inter poral distance, inter spinal distance, inter spinal outline had been observed in Tragopogon grcilis but length of spines is maximum in Tragopogon dubius. The study demonstrates potential of pollen studies in distinguishing some taxonomic groups in the Asteraceae.