Major aroma used in food, cosmetics, and pharmaceutical industries is chemically synthesized vanillin. The current research was carried out to explore local bacterial isolates for vanillin production using waste residues of rice bran oil. After the screening of different bacterial isolates, a novel strain Enterobacter hormaechei showed significant production of vanillin when inoculated in waste residues of rice bran based media. E. hormaechei produced 5.2 g/l of vanillin in culture media containing ferulic acid as a substrate. Optimum pH for E. hormaechei was 7 and the temperature was 30°C. Using optimum growth conditions and suitable fermentation medium, E. hormaechei can be used at an industrial level as producer of vanillin. Bioconversion of waste residues of rice bran oil to vanillin by bacterial strains is of greater interest nowadays. Bioproduction of vanillin is not only considered natural but also safe for human health.