Induction and growth of callus on shoot segments of Juniperus plycarpos was studied. Basal medium used was that of Murashige and Skoog's. The effect of auxins, kinetin, coconut milk and casein hydrolysate was studied on induction and further growth of callus. Auxins were NAA (0.05, 0.1, 0.5 and 1.0 mg/1). Cytokinin used was BAP (1.0mg/1). Our results indicated that size of the explant, presence or absence of apical bud, controlled conditions of light and temperature are important factors in callus formation. A successful callus could only be raised on MS medium supplemented with 1.0mg/1 of 2, 4-D, CM10%, sucrose 2%in diffused light at 25 ± 20°C in shoot segments having apical bud. For further growth of callus, addition of1.0mg/1 of BAP to this medium was beneficial.