Protein bound to acid detergent fiber (ADF-P) and in sacco digestibility of protein (ISPD) were determined in five wild (Acacia modesta, Pistacia attlantica, Pistacia khinjk, Olea cuspidate and Zizyphus mauritiana) and five cultivated (Prunus dulcis, Pyrus malees, Prunus persica, P. domestica and Punica granatumiree leaves collected from three different locations in Balochistan. Tree species significantly influenced ADF-P (P<0.001) and ISPD (P<0.001) of the leaves. ADF-P was higher (P<0.01) in wild than cultivated tree leaves (19.23% vs 9.01%). Conversely, ISPD was higher (P<0.001) in cultivated than wild tree leaves (54% vs 18%). A negative relationship between ADF-P in tree leaves may be done of the major factor limiting bio-availability of protein. It is concluded that in tree leaves crude protein is not a good indicator of their protein value and in addition the leaves should be analyzed for ADF-P and or in Sacco digestibility.