Toxoplasmosis is a disease that has been reported from all over the world. The objective of the current study was to evaluate the prevalence of Immunoglobulin-G (IgG) antibodies specific to Toxoplasma gondii (T. gondii) simultaneously in sheep, goat and humans associated with the sheep and goat farming from Bahawalpur, Pakistan from May, 2016 to April 2017. 640 blood samples were taken from sheep, goats, farmers and non-farmer humans (160 each). Blood sera were examined to detect anti-Toxoplasma antibodies (IgG) by employing latex agglutination test (LAT). The findings showed the seroprevalence of T. gondii 36.25% (58/160) for sheep, 28.1% (45/160) for goats, 21.2% (34/160) for farmers and 6.8% (11/160) for non-farmers. A significant difference (P-value 0.00; OR: 3.655; CL: 1.77-7.51) was found between seroprevalence of toxoplasmosis in farmers and non-farmers. Furthermore, the infection rates higher in females as compared with male subjects. The results suggested that milk and meat of infected sheep and goats might be responsible for transmission of T. gondii from sheep and goats to farmers of study area.