Vol.50, Iss. 5

In Vitro Culture and Biological Characteristics of Sheep Amniotic Mesenchymal Stem Cells

Tengfei Lu, Wenhua Pei, Shuang Zhang, Yangnan Wu, Fenghao Chen, Xiao Han et al.

With the self-renewal capacity and the potentiality of differentiating into various cells, stem cell is a prospective source for tissue engineering. Amniotic mesenchymal stem cells (AMSCs) obtained from amnion do not evoke ethical issues, as they do with the e…

Impact of Mycotoxin Binders on Humoral Immunity, Lymphoid Organs and Growth Performance of Broilers

Hazrat Nabi, Irshad Hussain, Muhammad Adil, Amar Nasir, Arbab Sikandar, Saeed Khan et al.

This project was designed to evaluate the impact of two commercial mycotoxin binders (Mycotox® and Mycofix® Plus 3.0) on the humoral immunity, lymphoid organs and growth performance of broilers. In this study, a total of 118 day-old broiler chicks were…

Efficacy of Probiotics Supplementation on Mineral Digestibility, Haematological Parameters and Carcass Composition of Oreochromis niloticus Fingerlings Fed Canola Meal Based Diets

Syed Makhdoom Hussain, Nosheen Aslam, Arshad Javid, Shehzadi Liaquat, Muhammad Mudassar Shahzad, Muhammad Zubair-ul-Hassan Arsalan et al.

Present trial was conducted to evaluate effects of probiotics on mineral absorption, haematology and carcass composition of Oreochromis niloticus fingerlings fed canola meal-based diet. Six test diets were prepared by using graded levels of probiotics (Protexi…

Diversity and Altitudinal Distribution of Squamata in Two Distinct Ecological Zones of Dir, A Himalayan Sub-Zone of Northern Pakistan

Qaisar Jamal, Muhammad Idrees, Saif Ullah, Muhammad Adnan, Farrah Zaidi, Qaiser Zaman et al.

Herpetological fauna of Dir Lower and Dir Upper districts (Northern mountainous region) of Khyber Pakhtunkhwa Pakistan contains 11 species of lizards (Calotes versicolor farooqi, Laudakia agrorensis, Eublepharis macularius, Cyrtopodion scabrum, Hemidactylus br…

Development and Evaluation of Clostridium perfringens Type D Toxoid Vaccines

Mumtaz Ali Khan, Aneela Zameer Durrani, Sher Bahadar Khan, Shehla Gul Bokhari, Ikramul Haq, Imdad Ullah Khan et al.

The aim of this project was to prepare a toxoid vaccine from the prevailing pathogenic strains of Type D Clostridiumperfringens strains and evaluate its immune responses in rabbits, goats and sheep. C. perfringens were isolated from enterotoxaemia suspected sh…

Effect of Temperature on Demography and Predation Rate of Menochilus sexmaculatus (Coleoptera: Coccinellidae) Reared on Phenacoccus solenopsis (Hemiptera: Pseudococcidae)

Ayesha Iftikhar, Muhammad Asif Aziz, Muhammad Naeem, Munir Ahmad, Tariq Mukhtar

Zigzag beetle, Menochilus sexmaculatus Fabricius (Coleoptera: Coccinellidae) is an important predator of cotton mealy bug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae). Understanding the effect of temperature variations on its demography and pred…

Enhanced Production of an Extracellular Lipase by EMS and MMS-Induced Mutant Strain of Rhizopus oligosporus EM-7 using Almond Meal as a Basal Substrate

Amna Waseem, Sikander Ali, Syeda Wajiha Khalid

The present study describes the screening and production of extracellular lipase (EC 3.1.1.3) from a Rhizopus oligosporus mutant strain developed by chemical mutagenesis. The wild-type was simultaneously treated with different concentration of EMS and MMS. Amo…

Seroprevalence and Immunization Program of Serotype O of Foot-and-Mouth Disease Virus in Pigs in Zhejiang Province, China

Bo Wu, Hui Zhang, Kun Li, Khalid Mehmood, Yan Zhao, Bin Jiang et al.

Foot-and-mouth disease (FMD) is one of the important diseases of pigs which often lead to a hazardous epidemic and causes serious economic losses to the pig industry. Currently, the epidemiological situation of FMD is uncertain in many regions of China and man…

Reactive Oxygen Species: Synthesis and Their Relationship with Cancer-A Review

Ayesha Noreen, Dilara A. Bukhari, Abdul Rehman

The reactive oxygen species (ROS) can be generated by intake of environmental pollutants, smoke, tobacco, xenobiotic, drugs, medical materials, radiations, pesticides, industrial solvents and ozone. The processes running in the cell membranes, peroxisomes, mit…

Comparative Performance of Jersey Sired Calves from Achai Dams and Azakheli Buffalo Calves Fed with Milk Replacer

Azmat Hayat Khan Khattak, Muhammad Nauman ul Islam, Muhammad Shakir Khan, Muhammad Iqbal, Imtiaz Ali Shah, Sheeraz Ghani et al.

Eight Jersey sired calves from Achai dams (AJC) and eleven Azakheli buffalo calves (ABC) were reared on milk replacer to compare their growth performance and economics. The experiment was performed at Livestock Research and Development Station (LR&DS), Sur…

Teratogenesis Induced by Trimethoprim Sulfamethoxazole in Mice

Madeeha Arshad, Asmatullah, Chaman Ara, Shagufta Andleeb, Naveed Ahmad

Antibiotic therapy is the most common for treating the UTIs during gestation, Trimethoprim Sulfamethoxazole (TMP-SMX) is one of antifolate used in such therapies. Current study was done to test the ability of TMP-SMX to induce developmental defects in mice fet…

Homozygous Nonsense Mutation in the ASPM Gene Causes MCPH in Consanguineous Pakistani Families

Ansar Ahmed Abbasi, Kathrin Blasius2, Imtiaz Ahmed, Hao Hu, Sylvie Picker-Minh2, Muhammad Nasim Khan et al.

Autosomal recessive primary microcephaly (MCPH) is a rare neurodevelopmental disorder characterized by severe reduction in the brain volume that affects especially the neocortex, and intellectual disability. To date 17 genes have been linked with this disease.…

Male Catfish Rita rita has higher Haemoglobin Concentration than Female Fish

S. Jalbani, N. T. Narejo, P. Khan, Y.M. Jalbani

Hematological analysis of blood of Rita rita, showed significant differences between total erythrocytes count, hemoglobin concentration and erythrocyte sedimentation rate of the two sexes. Male fish had total erythrocytes 5.05 ± 0.5 × 106 and hemo…

Damaging Effect of Gall-Forming Insects (Diptera: Cecidomyiidae) on Castanopsis hystrix in China

Xia Lin Zheng, Zhen Hua Xian, Zhen De Yang, Xiu Hao Yang, Wen Lu

Damaging effect of two species of gall midges (Diptera: Cecidomyiidae) have been investigated on Castanopsis hystrix Miq. (Fagales: Fagaceae) in Pubei County of Guangxi, China were investigated from December 2012 to December 2013. Jaapiella sp. damaged leaves,…

Effects of Declawed Males on Laying Performances, Egg Quality Characteristics and Feather Cover of Layer Breeders in the Mating Cage

Yan Li, Kai Zhan, Junying Li, Wei Liu, Ruiyu Ma, Shengnan Liu et al.

This study aimed to investigate the effects of declawed males on laying performances, egg quality characteristics and feather cover of layer breeders in the mating cage. Ninety-six 230-day-old Hy-line Brown roosters were randomly split into four groups. Group…

Molecular Characterization and Phylogenetic Analysis of Fowl Adenoviruses Isolated from Commercial Poultry Flocks in Pakistan during 2014-15

Abdul Wajid, Asma Basharat, Muhammad Akbar Shahid, Sidra Tul Muntaha, Abdul Basit, Tanveer Hussain et al.

Fowl adenovirus has been reported in many countries and is a contagious agent related with inclusion body hepatitis (IBH) and hydropericardium syndrome (HPS) in chickens worldwide. Identification of fowl adenovirus (FAdV) serotype is of most importance in epid…

Effect of Supplementation of Mannan-Oligosaccharides on Growth Performance, Viscera Development, Mineral Absorption and Caecal Microbiota of Japanese Quail (Coturnix coturnix japonica)

Muhammad Anwar Iqbal, Habib Rehman, Ali Hussain, Faiza Jabeen, Ijaz Ahmad, Kamran Ashraf et al.

This study was conducted to investigate the effect of mannan-oligosaccharides (MOS) as nutritional supplementation on zoo-technical parameters, visceral organ weights, serum mineral levels and caecal concentrations of E. coli and Clostridium spp. in Japanese q…

Exclusive Characteristics of the Bhag Nari Cattle among the Other Indigenous Cattle Breeds of Pakistan

Irshad Ali, Mohammad Masood Tariq, Abdul Waheed, Ferhet Abbas Yousafzai, Farhat Abbas Bokhari, Majed Rafeeq et al.

The base line study carried out on Bhag Nari (BN) cattle breed to explore morphological, biometric parameters, its production system, and survival in the existing habitat. The data on morphology and performance of 1020 Bhag Nari (BN) cattle were collected…

Bioinformatics Analyses of Bovine Adipose Tissue Transcriptome from Lilu Beef Cattle at Different Stages of Growth

Guifen Liu, Xiaomu Liu, Khuram Shahzad, Wei You, Juan J. Loor, Fachun Wan

The accumulation of adipose tissue is largely influenced by the genetic background of cattle. Therefore, we analyzed the transcriptome changes within adipose tissue in beef cattle at different stages of growth. Microarray analysis of data at 12 vs 18 months, 1…

Replacement of Fish Meal with Moringa oleifera Leaf Meal (MOLM) and its Effect on Growth Performance and Nutrient Digestibility in Labeo rohita Fingerlings

Syed Makhdoom Hussain, Muhammad Zubair ul Hassan Arsalan, Arshad Javid, Abdullah Ijaz Hussain, Nosheen Aslam, Qasim Ali et al.

A 70-day feeding trial was conducted to evaluate the effects of MOLM on growth performance and nutrient digestibility in L. rohita fingerlings. MOLM was added in the diet by replacing fish meal at the levels of 0%, 10%, 20%, 30% and 40%. Triplicate tanks were…

Nucleotide Polymorphism of Leptin Gene in Anatolian Water Buffaloes

Selçuk Kaplan

Marker assisted selection (MAS) have been using to determine the associations between the genes and economic traits of livestock animals. However, phenotypic values do not generally indicate the real genotypic values of animals. However, using MAS approaches i…

An Antimicrobial Cerebroside from the Liposoluble Constituent of Cervus Nippon Antler Velvet Layer

Nina Bao, Yongguang Yin, Panpan Wang

Using petroleum ether to extract an antimicrobial cerebroside from the liposoluble constituent of cervus nippon antler velvet layer was studied in this paper. Single-factor experiment was used to research the effect of liquid-to-solid ratio, standing time and…

Both Quorum Sensing (Qs)-I and Ii Systems Regulate Escherichia coli Flagellin Expression

Yang Yang, Yun Liu, Mingxu Zhou, Guoqiang Zhu

For elucidating effect of quorum sensing (QS) systems I and II (QS-1 and QS-II) on Escherichia coli flagellin expression, E. coli F18ab strain 107/86 was modified to either express acyl-homoserine lactone (AHL) synthase (QS-I) or deleted for autoinducer 2 (AI-…

Rapid and Simple Detection of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) by Reverse Transcription Loop-Mediated Isothermal Amplification

Xi-wen Chen, Qian Wang, Miao Yin, Zhong-hui Pu, Ai-wei Guo, Lian Li et al.

Given the economic consequences of infection with porcine reproductive and respiratory syndrome virus (PRRSV) for the global swine industry a rapid and practical early detection assay is required. This study established a reverse transcription-loop-mediated is…

The Effect of Different Preparation Methods on the Flavor of Farmed Takifugu obscurus

Zhuanxia Chen, Ningping Tao, Xiao Qin, Dayong Liu

The aim of this study was to investigate the tastes of the meat, liver and soup through different preparatory procedures which were used in traditional. The taste of Takifugu obscurus under different conditions were analyzed by sensory evaluation and electroni…

Reconstruction and Subcellular Localization Analysis of Eriocheir sinensis Molting Protein-Protein Interaction Network

Bin Wang, Qianji Ning, Qian Wang, Wei Peng, Tong Hao, Jinsheng Sun

Molting abnormalities of Chinese Mitten Crab (Eriocheir sinensis) can seriously affect their growth, causing significant losses to the aquaculture industry. The current studies on the molting mechanism mainly focused on the independent analysis of single prote…

Pilot-Scale Electrochemical Treatment of Textile Effluent and its Toxicological Assessment for Tilapia (Oreochromis niloticus L.) Culture

Muhammad Anwar-ul-Haq, Amer Jamal Hashmat, Ejazul Islam, Muhammad Afzal, Abid Mahmood, Muhammad Ibrahim et al.

The present study deals with the treatment of textile effluent using electrochemical techniques on pilot scale (70 L). One of the major problems encountered in the textile industry is the production of large volumes of highly colored wastewater. The scope of t…

Seroprevalence of Avian Influenza (H5) in Broilers from Five Districts of Khyber Pakhtunkhwa, Pakistan

Ali Zaman, Abdul Haleem, Sajjad Ur Rahman, Adnan Amin3 Asma Saeed, Sana Ullah, Naimat Ullah

Avian influenza (H5) is highly contagious infection of birds as well as humans which is zoonotic of significant economic and public health concern. From total 650 broilers sera were taken, around the five geographically distinct districts and analyzed through…

Genetic Differentiation and Relationship between Two Karyotype Forms of Nannospalax ehrenbergi (Rodentia: Spalacidae) in Egypt

Adel A. Basyouny Shahin, Amira M. Metwally Mohamed, Abd El-Reheem A. El Shatter

Genetic differentiation between the two karyotype forms I (2n = 60) and II (2n = 62), which have been recently recognized in the Egyptian N. ehrenbergi, was examined at 17 structural genetic loci and interrelationship was discussed. Of these 17 genetic loci, 1…

The Exploration of Beijing Fatty Chicken Amniotic Fluid Stem Cells with Multilineage Potential

Mingming Ning, Yanjie Zheng, Yuanyuan Dun, Weijun Guan, Xiuxia Li

This test aim at exploring and optimizing the isolation and culture methods of chicken amniotic fluid stem cells (CAFSCs) in vitro, characterizing CAFSCs and inducing CAFSCs to differentiate into three layers. Methods and results include that more CAFSCs are g…

Chicken Embryo Fibroblasts are Effective Cells to Study the Function of Chicken Neurexophilin 1 Gene

Dongmei Sun, Meiling Chen, Hafiz Ishfaq Ahmad, Yun Liu, Xunping Jiang, Guiqiong Liu

Neurexophilin has been proved to be a differentially expressed gene in chicken sperm storage tubules. In this work, Immunocytochemistry, immunofluorescence and Western-blot were performed to detect the expression of neurexophilin 1 in epithelial cells of chick…

Effect of Diverse Factors on the Frequency of Clinical and Subclinical Mastitis in Kundhi Buffaloes of Sindh, Pakistan

Hasina Baloch, Rahmatullah Rind, Muhammad Rafique Rind, Viram Kumar, Nazia Baloch, Rajesh K. Oad

The present study was aimed at determining the effects of diverse factors associated with clinical and subclinical mastitis in buffaloes. Significantly higher prevalence (49. 07%) of clinical mastitis was observed in buffaloes with average age of 6 to 9 years…

Comparative Evaluation of Selected Biorational Insecticides against Spodoptera litura (Fabricius) on Cauliflower

Sumaira Maqsood, Muhammad Afzal, Muhammad Anjum Aqueel, Waqas Wakil, Hafiz Azhar Ali Khan

Spodoptera litura is an economic pest of different field crops including cauliflower. In the present study three commercial formulations of biorational insecticides viz., Bacillus thuringiensis (DiPel®), NPV (SpltNPV) and Flubendiamide (Belt®) evaluate…

Construction and Evaluation of Novel Plasmid DNA Encoding Somatostatin Fused to a Tissue Plasminogen Activator (tPA) in Mice

Fangxiao Dong, Xuan Luo, Delu Yuan, Aixin Liang, Liguo Yang

Somatostatin (SS) DNA vaccine has been used to improve the growth and lactation of animals, however, the immune response is still unsatisfactory, especially in large animals. The present study aimed to investigate whether tissue plasminogen activator (tPA) cou…

The Fungicide Thiram may Disrupt Reproductive Cycle of Domestic Male Pigeon (Columba livia domestica) Subjected to a Long Photoperiod

Souheila Slimani, Sonia Hamouda, Chahrazed Souadi, Sara Silini, Cherif Abdennour, Leila Delimi

Fungicides are toxic chemicals, very much used in agriculture, but they are associated with the appearance of certain metabolic, carcinogenic, neurotoxic and fertility disorders. The objective of this work is to study the toxic effect of the dithiocarbamate &l…

Pork Jerky Fermented with Lactobacillus bulgaricus and Angel Yeast

Changqing Zhao, Zhuochi Chen, Yubin Li

Lactobacillus bulgaricus and Angel Yeast was used to produce fermented pork jerky, and a series of indexes were measured. The results demonstrated that the pH of the fermented jerky was lower than the non-fermented jerky; the true protein content of the fermen…

Effect of Short-Term Starvation on Serum Metabolites, Antioxidant Enzymes and Endogenous Reserves of Rainbow Trout, Oncorhynchus mykiss

Tayfun Karataş

This study was conducted to investigate the effects of starvation for 8 days on the serum metabolites and antioxidant enzymes, thiobarbituric acid reacting substance levels, endogenous reserves in liver and muscle tissues of rainbow trout (Oncorhynchus mykiss)…

Structure Based Virtual Screening and Molecular Docking Studies for Identification of Allosteric Inhibitors against Zika Virus Protease NS2B-NS3

Musrrat Fatima, Muhammad Saad Khan, Hamid Rashid, Asim Mehmood, Sumaira Kanwal, Muhammad Asif Rasheed et al.

Zika virus (ZIKV) has gained research interests after its recent outbreak in Brazil and America in 2015, where it affected millions of people. Studies have shown its role in developing microcephaly and GBS Syndrome. There is a need to develop drugs against ZIK…

The Efficacy and Persistence of Spodoptera littoralis Nucleopolyhedrovirus (SpliMNPV) Applied in UV Protectants against the Beet Armyworm, Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae) under Saudi Field Conditions

Sukirno Sukirno, Muhammad Tufail, Khawaja Ghulam Rasool, Said El Salamouny, Koko Dwi Sutanto, Abdulrahman Saad Aldawood

Baculoviruses are known biocontrol agents of several crop pests, however, their activity is deteriorated rapidly when exposed to the sunlight. Many UV protectants were used to improve the efficacy of these viruses under field conditions. The objective of the c…

Genetic Screening of COMT Gene for Aggression in Domestic Bovines of Pakistan

Maryam Javed, Farwa Saghir, Naveera Aziz, Maham Saeed, Asif Nadeem, Wasim Shehzad

Aggression in animals is mostly related to the territory protection, competition for resources like water, food, offspring and mating probabilities. It is an antisocial behavior having strong genetic relation. Current study focused mainly on the screening of g…

Identification and Isolation of the Specific Allergens from Mutton by Western Blot, Chromatography and MALDI-TOF/TOF-MS

Jia Chen, Yuanxi Deng, Hui Xu

To study the specific allergen in mutton, soluble protein from mutton was extracted, and separated by SDS-PAGE. With the positive and negative sera of rats, the special allergen was identified with the Western Blot, showing that the 12kDa was the specific alle…

Description of Factors Influencing Final Fattening Weight in Domestic Beef Cattle Breeds through Mars Algorithm

Yakup Erdal Ertürk

This paper deals with describing several influential factors that have significant impact on final fattening weight (FFW) as an output variable at domestic beef cattle enterprises from the Eastern part of Turkey. Multivariate adaptive regression splines (MARS)…

Reproductivity of Meloidogyne incognita on Fifteen Cucumber Cultivars

Muhammad Zameer Kayani, Tariq Mukhtar

Root-knot nematodes are mainly controlled by chemicals and their use is often coupled with health hazards. The deleterious effects of pesticides can be averted by using non-chemical approaches, and resistant cultivars can prove a promising substitute. For thei…

Correlation between Various Udder Measurements and Milk Components in Morkaraman, Tuj and Awassi Sheep

Doğan Türkyılmaz, Selçuk Özyürek, Nurinisa Esenbuğa, Mustafa Yaprak

In this study, it was aimed that to identify components of milk and udder type and to correlations between udder measurements and milk components in Morkaraman, Tuj and Awassi sheep. Milk components are investigated in order to determination of milk quality. T…

Improvement in Mineral Digestibility and Whole Body Composition of Catla catla Fingerlings Fed Phytase Supplemented MOSM Based Diet

Muhammad Mudassar Shahzad, Syed Makhdoom Hussain, Farhat Jabeen, Abdullah Ijaz Hussain, Arshad Javid, Muhammad Asrar et al.

Moringa oleifera seed meal (MOSM) was used as test ingredient to formulate six test diets and were supplemented with graded levels (0, 300, 600, 900, 1200 and 1500 FTU kg-1) of phytase. The fingerlings were fed at the rate of 4% of live fish body weight twice…

Characterization of Milk Proteins from Different Animal Species through Gel Electrophoresis

Nuzhat Huma, Fozia Ghaffar, Saima Rafiq, Imran Pasha, Aysha Sameen, Imran Hayat et al.

Present investigation covers the chemical composition, nitrogenous compounds and protein fractionations of casein and whey proteins of milk from different dairy animals like buffalo, cow, sheep, goat and camel. The buffalo and sheep milks have comparatively hi…

Occurrence of Rodent Species in Agricultural Lands during Cropping and Non-Cropping Seasons of Pothwar Plateau, Pakistan

Nadeem Munawar, Iftikhar Hussain, Tariq Mahmood

Rodents form an important component of biodiversity of the agricultural lands and have been identified as destructive mammalian pests of agricultural crops at global level. They cause severe damage to various crops, directly and indirectly, by gnawing, spoilag…

Table of Contents

The Microbial Community in the Feces of Cape Oryx (Oryx gazella) as Determined by High-throughput Illumina Sequencing Technology

Jun Chen, Shuai Shang, Xiaoyang Wu, Jiakuo Yan, Huaming Zhong, Huanxin Zhang et al.

As a ruminant herbivore, the cape oryx from southern Africa has a digestive system that allows it to absorb and digest large amounts of plant material through microbial fermentation in the hindgut. So far, there has been no study of the gut microbiota of the c…

Metarhizium baoshanense sp. nov., a New Entomopathogen Fungus from Southwestern China

Zihong Chen, Ling Xu, Xiaona Yang, Yaguan Zhang, Yuming Yang

Metarhizium is an entomopathogenic fungal genus relevant in biological protection. A new species of the genus, collected from Taibao mountain, Baoshan City, Yunnan Province, southwestern China, was described here as Metarhizium baoshanense. It was proposed and…

The Predominant Incidence of Mycoplasma mycoides subsp. capri in Suspected Cases of Contagious Caprine Pleuropneumonia in Sheep and Goats of Northern Pakistan

Hanif Ur Rahman, Umer Saddique, Zahoor-ul-Hassan, Shakoor Ahmad, Muhammad Kamal Shah, Said Sajjad Ali Shah et al.

Contagious Caprine Pleuropneumonia (CCPP), a highly contagious and economically devastating disease, is caused by Mycoplasma mycoides (MM) - a cluster of six member species. However, literature is not available regarding the causative agents of CCPP in sheep a…

Environmental Determinants of Zooplankton Community in the Damietta Estuary of the Nile River, Egypt

Wael S. El-Tohamy, Russell R. Hopcroft, Nagwa E.M. Abdel Aziz

Zooplankton community and eleven environmental variables were investigated seasonally during 2014 through nine stations in the Damietta estuary of the Nile River. Meroplanktonic larvae were the major component representing 39.4% of the total zooplankton abunda…