Vol.52, Iss. 6

Cytotoxic Effect of MTH1 Gene Silencing in Gemcitabine Resistant Breast Cancer Cells

Mahak Fatima, Memoona Syed, Rabia Zeeshan, Farah Rauf Shakoori, Naveed Shahzad, Moazzam Ali et al.

Nudix hydrolase 1 (NUDT1) or human MutT homolog 1 (MTH1) catalyzes the conversion of oxidized purine nucleotides to their monophosphate forms, thus preventing their incorporation into DNA and subsequently to oxidative damage. The present study explores the rel…

Do Seismic Signals Diverge at the Level of Cytotypes in Turkish Blind Mole Rats?

Faruk Çolak, Ferhat Matur

Subterranean animals are limited in mobility and have reduced sensory ability due to living underground, and therefore are presented with a challenge to communicate. One solution is to use seismic signalling, by head drumming, to convey species-specific inform…

Composition and Diversity of Dragonflies (Insecta: Odonata) in Tunan Waterfall Area, North Minahasa, North Sulawesi, Indonesia

Roni Koneri, Meis Nangoy, Pience V. Maabuat

Dragonflies play an important role in an ecosystem and can serve as control agents of agricultural insect pests. Dragonflies can be used as bioindicators for evaluating environmental changes in long-term studies (biogeography) and short-term studies (conservat…

Autumn Time Budget and Diurnal Rhythm of Black-Necked Crane

Tong Zhang, Feng Xu, Ming Ma, Peng Ding

In the autumn of 2011, both the time budget and diurnal rhythm of behavior of black-necked crane (Grus nigricollis) were studied at Altun Mountain National Nature Reserve, Xinjiang, China. The results showed that foraging was the main behavior of cranes in aut…

A New Species of the Genus Carniella Thaler & Steinberger from the Nepal Himalayas (Araneae: Theridiidae)

Andrei V. Tanasevitch, Yuri M. Marusik

A new species, Carniella nepalensis sp. n., is described from eastern Nepal. The male of new species is most similar to C. forficata (Gao and Li, 2014) and clearly differs by the shape of the embolus. The type locality of new species is northwesternmost for th…

Sex Ratio, Habitat Selection and Foraging Behavior of Mandarin Duck in Water Systems of Poyang Lake, China

He Wenyun, Zhi Yijin, Shao Mingqin

From February to March 2017, November 2017 to March 2018 and November 2018 to March 2019 we used the point count method to survey the sex ratio, habitat selection and foraging behavior of Mandarin duck (Aix galericulata) in water systems (Raohe River, Ganjiang…

Expression and Localization of VIP in Different Segments of Pigeon Intestine

Fei Wang, Xianlai Zhang, Youbao Zhong, Xiaoen Tang, Xiaofen Hu, ShanShan Yang et al.

In order to further investigate the structural characteristic of pigeon intestine, the microstructure of each part of intestine was analyzed by HE staining, and its vasoactive intestinal peptide (VIP) was detected by immunohistochemistry staining and RT-qPCR.…

Wading Bird Habitat, Water Depth Utilization and Niche Separation in Poyang Lake, China

Zhi Yijin, Shao Mingqin, Li Quanjiang

From October to December 2018, and March to May of 2019, habitat, water depth utilization and niche separation of wading birds in Poyang Lake, China, was assessed. A total of 38 bird species, including 4 class I and 3 class II Chinese nationally protected bird…

Bioconversion of Agricultural Wastes to Polyhydroxybutyrate by Azotobacter vinelandii

Tehmina Aslam, Shagufta Saeed, Muhammed Tayyab, Huma Mujahid, Ali Raza Awan, Sehrish Firyal et al.

The aim of the present study was to evaluate the potential of different agricultural wastes like wheat bran, rice polishing and molasses for the synthesis of polyhydroxybutyrate (PHB) by Azotobacter vinelandii. The optimization of different carbon sources and…

Efficacy of Danshen (Salvia miltiorrhiza) Dripping Pills for Prevention of Acute Mountain Sickness: A Randomized, Double-Blind, Placebo-Controlled Trial

Kui Li, Lijun Li, Jialun Fu, Yi He, Zhaohui Song, Shuiping Zhou et al.

A randomized, double-blind, placebo-controlled trial was conducted to investigate the efficacy of prophylaxis by administering compound Danshen dripping pills (CDDPs) on acute mountain sickness (AMS). To this end, a total number of 58 healthy low-altitude inha…

Evaluation of Different Brassica spp. Against Aphids, Under Multan Agro-Ecological Conditions

Mah Jabeen, Amjad Farooq

Four species of Brassica viz,. B. napus, B. juncea. B. compestris and B. rapa were studied for their relative resistance/susceptibility against aphids, under field conditions for three consecutive crop seasons i.e., 2009-2010, 2010-2011 and 2011-2012. The data…

Multiple Metal Resistant Bacillus cereus 3.1S Isolated from Industrial Effluent has Promising Arsenite Oxidizing Potential

Ayesha Noreen, Amina Elahi, Dilara Abbas Bukhari, Abdul Rehman

The arsenite resistant Bacillus cereus 3.1S, isolated from pesticide industry effluent, showed maximum growth at pH 7 at 37 °C in LB medium after 24 h of incubation. The strain tolerated As3+ up to 40 mM and also showed resistance against Pb2+ (8mM), Cd2+…

Rab21, an Endocytic Rab GTPase, is Involved in Drug Resistance in Prostate Cancer Cells

Maryam Yousaf, Naveed Shahzad, Zeeshan Mutahir, Moazzam Ali

Rab GTPases are important regulators of intracellular trafficking, having distinct intracellular localisation for controlling endocytic, exocytic, and recycling pathways. The impairments in Rab pathways have been linked with drug resistance in various cancers.…

Relationship between Giant Panda Ailuropoda melanoleuca Habitat use and Disturbance Density in the Daxiangling Mountains, Sichuan, China

Cheng Zhao, Jianghong Ran, Bin Wang, Qin Shi, Marwan M.A. Rashed

The giant panda Ailuropoda melanoleuca is one of the most iconic mammals in the world. The species has experienced declines in its habitat and population due to human disturbance. To protect this species, we investigated the relationship between giant panda ha…

The Use of Otolith Shape to Identify Stocks of Redlip Mullet, Liza haematocheilus

Tao He, Cheng-jing Chen, Jian-guang Qin, Yun Li, Rong-hua Wu, Tian-xiang Gao

The aim of this study was to compare sagittal morphometric features between Liza haematocheilus stocks in the China coasts at the Ying Kou, Dong Ying, Qing Dao, Wen Zhou and Guang Zhou regions and test a complementary method to quantify the variation of sagitt…

A Comparison of Somatic Condition and Testis Mass in Black-Spectacled Toad (Duttaphrynus melanostictus) between Two Populations at Different Altitudes

Duo Jing Qiu, Xin Yu, Mao Jun Zhong, Long Jin

Altitude, served as one common geographical gradient has been considered to have a major influence on the evolution of life-history traits, which leads to variation in temperature, food availability and duration of breeding season in amphibians. Here, we exami…

Characterization of Bioluminescent Bacterial Strain Isolated from Nemipterus japonicus Fish of Arabian Sea

Aribah Naz, Uzma Badar, Afsheen Arif, Zaid Qureshi, Sondas Saeed, Erum Shoeb et al.

Luminescent bacteria are the most widely distributed organisms among glowing creatures, many of which are found in sea water while some are found in fresh water or terrestrial habitat. Marine micro flora possesses unique features that help them to survive unde…

Environmental Characteristics in a Fish Farm with Copper Alloy Cage System in the Dardanelles

Sinan Uzundumlu, Yesim Buyukates, Murat Yigit, Musa Bulut, Rıdvan Kaan Gurses, Barbaros Celikkol

This study was conducted in a copper alloy cage fish farm to observe the environmental properties of the marine system. Water quality parameters such as temperature, salinity, pH and dissolved oxygen, total suspended solids, chlorophyll-a and inorganic nutrien…

Seasonality and Climatic Factors Affect Diversity and Distribution of Arthropods Around Wetlands

Waqar Majeed, Naureen Rana, Elmo Borges de Azevedo Koch, Shahla Nargis

The diversity and distribution of arthropods associated with seasons and environmental variables in two different wetlands sites was estimated and study was performed on monthly basis for a whole year. The arthropods were collected by sweep net, direct hand pi…

Variants of MT-RNR2, MT-TI and MT-TL1 Genes in Hypertrophic Cardiomyopathy Families of Pakistan

Muhammad Khan, Tehmina Ameer Khan, Aziz Ud Din, Muhammad Fiaz Khan, Irfan Ullah, Kalim Ullah et al.

Hypertrophic cardiomyopathy is a condition in which the myocardial muscles become thickened without an apparent cause. It exhibits the prevalence of 1/500 globally. Eighteen samples from four families of maternally inherited HCM were collected, based on their…

Physiological Effects of Ethanolic Leaf Extract of Duranta erecta (L) on Albino Rat, Rattus norvegicus

Gregory Ejikeme Odo, Juliana Ekenma Agwu, Nkechi Nweze, Clara Ikegbunam, Felicia Ekeh N, Vincent Ejere et al.

Thisstudyevaluated thephysiological effects of ethanolic leaf extracts of Duranta erecta (L) in 48 albino wistar rats, Rattus norvegicususingbiochemical and haematological indicesof toxicity. The data were subjected to two-way analysis of variance (ANOVA) with…

Effect of Different Application Methods on the Nematicidal Activity of Bio and Synthetic Chemicals against Root Knot Nematode, Meloidogyne incognita (Kofoid and White) Chitwood

Huma Abbas, Nazir Javed, Muhammad Kamran, Sajid Aleem Khan, Hira Abbas, Ehetisham-ul-Haq et al.

Meloidogyne incognita (Kofoid and White) Chitwood is the most widespread and destructive pathogen of tomato, difficult to control due to its wide host range. In present investigation effect of bio (Cure, Azadirachtin) and synthetic (Cartap,…

Effect of a Rehabilitation Garden on Rehabilitation Efficacy in Elderly Patients with Chronic Obstructive Pulmonary Disease

Yan Wen, Liu Lian, Zhuang Xun, Murong Jiaying, Cui Shuyi, He Wanwen et al.

The main objective of the present study was to evaluate the efficacy of an outdoor rehabilitation program performed in a garden setting for elderly patients affected with chronic obstructive pulmonary disease (COPD). The elderly patients suffering from COPD we…

Dynamic Changes in Bacteria and Water Quality and their Relationship with Survival Rate during Penaeus monodon Larva Culture

Chao Zhao, Sigang Fana, Lihua Qiua

Although shrimp larvae are susceptible to many pathogens and water quality and diseases affect commercial shrimp production, the relation between these factors are less studied. Therefore, we aimed to investigate the effect of bacterial dynamics and water qual…

Diversity and Distribution of Genera Schizothorax and Schizothorichthyes in River Swat, Pakistan

Muhammad R. Khan, Bushra A. Rakha, Muhammad S. Ansari

Morphometric characters and meristic counts of genera Shizothorax and Schizothorichthyes have been studied from River Swat, Pakistan. Monthly collection of both Genuses was conducted and identified on morphometric and meristic counts and fin formula. The minim…

Molecular Identification of Pakistani Lathy Rock Pigeon using Cytochrome B Gene

Sehrish Firyal, Ali Raza Awan, Muhammad Umair Latif, Muhammad Tayyab, Muhammad Wasim, Shagufta Saeed et al.

Single nucleotide polymorphism (SNPs) analysis is a powerful tool for taxonomic and phylogenetic analysis. These are bi-allelic molecular markers, easy to interpret and uniformly distributed within genomes. In this study cytochrome b (Cytb) gene based SNPs ana…

Effect of Sublethal Doses of Bifenthrin and Chlorpyrifos Administered Alone and in Combinations on Esterases of Stored Grain Pest, Trogoderma granarium

Anum Feroz, Abdul Rauf Shakoori, Farah Rauf Shakoori

Toxic effect of sub-lethal concentration (LC20) of bifenthrin, chlorpyrifos and their combinations was studied on the specific activities of esterases in the 4th and 6th instar larvae of susceptible laboratory strain (Lab-S) and deltamethrin resistant populati…

Variation in Physiological Biomarkers with Different Clostridium perfringens Isolate Infections in Balkhi Sheep

Mumtaz Ali Khan, Sher Bahadar Khan, Shakoor Ahmad, Irshad Ahmad, Kashif Prince, Ghazunfar Rashid et al.

The objective of this study was to determine variations of different physiological biomarkers in Clostridium perfringens (CP) infections in Balkhi breed of sheep in Khyber Pakhtunkhwa, Pakistan. Study was conducted on 184 sheep suspected of having enterotoxemi…

Construction and Functional Verification of siRNA Eukaryotic Expression Vector Directed at the Follicular Inhibin Alpha Gene in Ye Mule Sheep

Zengwen Huang, WuReliHazi Hazihan, Baheti Bodai, Kadyken Rizabek, Nuralieva Ulzhan, Omarova Karlygash et al.

The Ye Mule Aries (YM) Sheep is a valuable local sheep breed from Xinjiang, China. This breed is well adapted to harsh environmental conditions and displays strong disease resistance, fast growth, and high cold tolerance. The effect of small interfering RNA (s…

Associations of Diplotypes of Vldlr Gene with Egg Production Traits in Laiwu Black Chickens

Yan Zhou, Hai Xia Han, Qiu Xia Lei, Jin Bo Gao, Wei Liu, Fu Wei Li et al.

Very low density lipoprotein receptor (VLDLR) is of vital importance for egg production in mediating the synthesis of yolk protein precursors. To better understand the effects of VLDLR on reproduction in chickens, the haplotypes and diplotypes based on three g…

Debunking Myths about COVID-19, Paranoiac Misconceptions, Recent Developments and its Current Stance

Zumama Khalid, Muhammad Abrar Yousaf, Abeedha Tu-Allah Khan, Farah Rauf Shakoori, Muhammad Munir, Abdul Rauf Shakoori

As the COVID-19 is becoming an increasing health threat, it has urged the scientists all over the world to not only research about its transmission, prevalence and causes but also to spread its awareness in the maximum possible ways. Although the complete dise…

Determination of Heterocyclic Amines in Ready to Eat Chicken Kabab Commercially Available from Pakistani Markets

Ishrat Perveen, Yasar Saleem, Javed Iqbal Qazi

Presence of heterocyclic amines (HCAs) in high proteinaceous food specifically in cooked meats is a point of great concern for the health risk factor of Pakistani community. Ready-to-eat (RTE) chicken kabab samples of four commercially available brands (K, S,…

Prevalence of Tick Born Babesia Infection in Domestic Cattle of Khyber Pakhtunkhwa, Pakistan

Aamir Khan, Rabia Noushin, Mohammad Attaullah, Shahid Naiz Khan, Rafiqe Hussain, Farman Ullah Dawar et al.

Looking into the importance of tick born diseases in the livestock sector, the present study was conducted to find out the prevalence of babesiosis among domestic cattle of Southern Khyber Pakhtunkhwa. Blood samples (1500) were collected from clinically suspec…

Genetic Association of Two Novel SNPs in CYP7A1 Gene with Lipid Traits of Tianzhu Black Muscovy (Cairina moschata)

Guang-Hui Tan, Yi-Yu Zhang, Yuan-Yu Qin, Lei Wu, Jie-Zhang Li

Cholesterol 7α-hydroxylase (CYP7A1) plays a crucial role in the synthesis of bile acid, fatty acid and cholesterol metabolism in human and rodent. However, a little was known in the literature on the question of the effect of CYP7A1 gene on poultry lipid…

Determination of Number and Position of Knots in Cubic Spline Regression for Modeling Individual Lactation Curves in Three Different Breed

Samet Hasan Abaci, Hasan Onder, Mustafa Sahin, Esra Yavuz

Lactation curves are one of the basic tools in animal breeding. Therefore, modeling lactation curves with appropriate and precise equations are of great importance for obtaining estimates. Lactation curves have different tendencies in different breeds. To exam…

The Effect of Different Levels of Matricaria chamomilla on Functional Status of the Liver in Rats Injected with Carbon Tetrachloride

Magbolah Salem Helal Alzahrani

Chamomile is a member of the Asteraceae family and has been well established as a medicinal plant. There are many different species of chamomile, the two most common being German chamomile (Marticaria chamomilla) and Roman chamomile (Chamaemelum nobile). The u…

Concentration Dependent Functions of FGF10 in Angiogenesis and Axon Guidance

Fang Liu

Previous studies suggest FGF10, which is expressed in the ventral hypothalamus, plays important roles in axon guidance and in vasculature guidance of the developing hypothalamus. Here, using ex vivo analyses in chick, we show that FGF10 exerts concentration de…

Evaluation of Different IgY Preparation Methods and Storage Stability as Potential Animal Feed Supplement

Shikun Ge, Yanli Yang, Brindha Chelliappan, Antonysamy Michael, Fagang Zhong, Xiaoying Zhang

Egg yolk can be used as feed additives to improve the immune status of animals and as passive therapy for veterinary diseases, and the egg yolk has proved to be a promising source of antibodies. However, the extraction methods and storage stability of IgY anti…

Length-Weight Relationship and Growth of a Marine Gastropod Mollusk, Hemifusus ternatanus (Gmelin) (Family: Melongenidae)

Shouguo Yang, Fen Cheng, Xiangyu Wu, YiXuan Xing, ZhiFeng Gu, Xianming Tang

Length-weight relationship and growth of a marine gastropod mollusk, Hemifusus ternatanus was studied for 36 months under artificial condition. The total shell length (SL), shell width (SW) and total weight (TW) were measured for eight times throughout the exp…

Comparison of the Gut Microbiota in the Tibetan Wild Ass (Equus kiang) Collected from High and Low Altitude

Honghai Zhang, Yao Chen, Xiaoyang Wu, Shuai Shang, Jun Chen, Jiakuo Yan et al.

The gut microbiota plays an important role in animal performance and the environment. We collected fresh feces of the Tibetan wild ass (Equus kiang) from the Jinan Wildlife World and Qinghai - Tibet Plateau Wild Animal Park. And we divide the sample into Group…

Antimicrobial Properties of Aloe Vera Gel Extracts against Bacterial Isolates from Wound of Donkey

Safia Arbab, Rehana Buriro, Shams Uddin Bhugio, Atta Hussain Shah, Jamila Soomro, Qudratullah Kalwar et al.

Despite the exploration of antibacterial activity of the Aloe Vera in different families of animals. However, very limited information is documented about antibacterial activity of Aloe Vera in equines. The present study was designed to check the antibacterial…

Description of Amphibelondira sindhicus N. Sp. with Observation on Belondira paraclava Jairajpuri, 1964 (Family Belondiridae) from Sindh, Pakistan

Ashfaque Ahmed Nahiyoon, Nasira Kazi, Shahina Fayyaz

A new and a known species belonging to the family Belondiridae are described from Sindh, Pakistan. Amphibelondira sindhicus n. sp., is characterized by having reduced anterior genital branch, more slender body, smaller neck length and expanded part of pharynx,…

Impact of Two Attending Ants, Crematogaster subnuda and Camponotus compressus (Hymenoptera: Formicidae), on the Parasitism of Sugarcane Aphid Melanaphis sacchari (Zehnt.)

Zubair Ahmad, Hamed A. Ghramh, Khalid Ali Khan, Farhat Khan, Shujauddin

The mutualistic relation between ants and aphids is considered as one of the best examples of inter-specific mutualism between species that produces net benefits for the participants. In this paper, the interaction between aphid M. sacchari and their attending…

An Unusal Case of Hydramnios in Nili Ravi Buffalo

Shahid Ali, Aijaz Ali Channa, Waqar Ahmad, Rafia Akram, Imbasat Yousaf

Hydramnios was successfully managed in a full term pluriparous Nili Ravi buffalo (Bubalus bubalis) that was recumbent and under extreme stress because of intemperate bilateral abdominal distension. Trans-rectal palpation revealed a taut uterus (bulged, tense a…

Responses of Captive Ostrich Struthio camelus to Zoo Visitors at Central Zoo, Lalitpur, Nepal

Hari Prasad Sharma, Shova Adhikari, Yogesh Rai, Rajkumar Sijapati, Sapana Chand, Manisha Karki et al.

Zoos are established to engage the general public in informal education, research and conservation, as well as maintaining their role as attractive places for entertainment and recreation. Zoos provide home for different animals that are of common occurrence,…

Three New Species of the Genus Microplitis Förster, 1862 (Hymenoptera: Braconidae: Microgastrinae) from Saudi Arabia

Hamed A. Ghramh, Zubair Ahmad, Khalid Ali Khan, Farhat Khan

In this study, Microplitis tihamicus sp. nov., M. faifaicus sp. nov. and M. khamisicussp. nov. (Hymenoptera: Braconidae: Microgastrinae) are described. The genus Microplitis is recorded for the first time from Saudi Arabia. Characters of these new species and…

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Bioaccumulation of Metals in the Organs of Fish inhabiting Ravi River: Serious Threat to Fish and Consumer’s Health

Zarish Yaqoob, Saleema Bashir Shams, Gaitee Joshua, Bibi Nazia Murtaza

Heavy metal contamination of river water adversely affects the growth and productivity of fish and contributes to the human health disasters. River is a trans-country river originating in Himachal Pradesh, India and joins Indus and its tributary rivers in the…

UVB Irradiation Induces Human Skin Fibroblasts Senescence Through Regulation of Β-Catenin and Smp30 Expression

Feng-Mei Yang, Rui Li, Xiu-Xue Hu, Yong Liang, Bo Gao, Wei Chen

This study aims at measuring expressions of β-catenin and senescence marker protein-30 (SMP30) after normal human skin fibroblasts (NHSFs) irradiated by different UVB doses as well as durations, thus unveiling mechanisms underlying HSF senescence induced…

Comparison of Models for some Testicular Characteristics in Karakaş Male Lambs

Kadir Karakuş, Turgut Aygün, Åženol Çelik, Mohammad Masood Tariq, Muhammad Ali, Majed Rafeeq et al.

This study was aimed to search testis growth of Karakaş male lambs raised in intensive conditions and to build the forecasting model of live weight in lambs using 4 data mining CHAID, Exhaustive CHAID, CART, and multivariate adaptive regression splines (MARS).…

Microarray and Bioinformatics Analysis of Differentially-Expressed MicroRNAs in Wnt/Β-Catenin Signaling-Activated Bovine Skeletal Muscle Satellite Cells

Ming-Yan Shi, Xu-Liang Yan, Ping-Ping Han, Yu-Jie Tang, Hui-Xia Li

Skeletal muscle satellite cells (SMSC) are a population of multipotent cells that exist not only during animal infancy, but are also present in the adult mammalian body. Previous studies have indicated that Wnt/β-catenin signaling is involved in the regul…

Seasonal Variation in Ecdysis Process and Parasitic Prevalence in Indian Rock Python (Python molurus)

Sumaira, Syed Mohsin Bukhari, Maqsood Iram, Arshad Javid, Ali Hussain, Waqas Ali et al.

The effects of feed and environmental factors have been studied in ecdysis process in Indian rock python. Ten experimental animals were observed in captivity and it was noted that ecdysis was more rapid during summer compared to winter. Furthermore, ticks and…

Molecular Evidence for the Association of Swarm Forming Desert Locust, Schistocerca gregaria gregaria (Forskål) in Pakistan with Highly Prevalent Subspecies in Sahara Desert of Africa

Jam Nazeer Ahmad, Samina J.N. Ahmad, Mubashir Ahmad Malik, Abid Ali, Muhammad Ali, Ejaz Ahmad et al.

Locust commonly known as short horned grasshopper is one of the most dangerous agricultural pests worldwide. There are various important species of large swarms forming of desert locusts found in different regions of the world. In early 2020, in Pakistan, huge…

Chelonine (Hymenoptera: Braconidae: Cheloninae) Parasitoids of Lepidopteran Leafminers in the Indian Subcontinent, with Notes on Taxonomic Character

Zubair Ahmad, Hamed A. Ghramh, Khalid Ali Khan, Kavita Pandey, Farhat Khan

Two new species viz., Chelonus (Areselonus) caeruleus sp. nov. and Chelonus (Areselonus) lithocolletiscus, sp. nov., are described as new to science from the northern part of India. These two species were reared from Acrocercops caerulea (Meyrick) and Lithocol…