Vol.53, Iss. 4

Ecology of Fish Communities in Coral Habitats Along the Coast of Pakistan: Potential Threats and Conservation Strategies

Amjad Ali, Pirzada Jamal Ahmad Siddiqui, Naveed Ahmad, Shabir Ali Amir, Rafaqat Masroor, Seema Shafique et al.

Reef ecosystem is an important source of recreation and fish diversity. Present study aimed to record the distribution and diversity of fishes from different coral habitats along the coast of Pakistan. Additionally, potential threats and conservation strategie…

Study on Change of Wool Fineness with Age of Alxa Bactrian Camel

Junyan Bai, Ren Tao Di Wu, Le Ma Dao, Di Bao, Qiang Zhang, Xing Hua Tian

In this study, SPSS17.0 software was used to analyze the wool fineness of 1079 Alxa Bactrian camels. The results showed that the wool fineness of male camel was significantly higher than that of female camel (P < 0.05). At the age of 2-7, the fluff fineness…

Relationship of a Novel c.429delC Deletion in Hairless Gene HR with Alopecia in Two Families from Southern Punjab, Pakistan

Muhammad Ajmal, Saima Mustafa, Fizza Ibrahim Bajwa, Cheng Zhou, Guangdong Wen, Soe Lwin Myint et al.

Atrichia with papular lesions (APL) is a rare autosomal recessive form of total alopecia, characterized by hair loss soon after birth and the development of papular lesions of keratin-filled cysts over extensive areas of the body. Two consanguineous families w…

Identification of Cotton Physio-Morphological Marker for the Development of Cotton Resistant Varieties against Sucking Insect Pests: A Biorational Approach for Insect-Pest Management

Saima Siddiqui, Ghulam Hussain Abro, Tajwar Sultana Syed, Abdul Sattar Buriro, Sohail Ahmad, Muhammad Zeeshan Majeed et al.

Cotton is one of the important crops of Pakistan and is attacked by many chewing and sucking pest. Chemical insecticides are used abundantly to suppress the pest population. The use of host-plant resistance is an alternative tool to control cotton insect-pests…

Effects of Storage Temperature on the Microbiological Quality of Fish Meat from Two Different Managemental Systems

Rida Haroon Durrani, Fareeha Akhtar, Ali Ahmad Sheikh, Munazzah Maqbool, Aleena Kokab, Kiran Imtiaz et al.

Food spoilage is a complex process in which food deteriorates to that extent at which it becomes unfit for human consumption. Food spoilage due to microorganisms is a serious issue which causes approximately 25% food loss globally Present study revealed the ef…

The Species Identity of the Two Color Morphs of Northern Snakehead (Channa argus) Based on mtDNA Control Region Sequences

Aiguo Zhou, Di Sun, Shulin Liu, Yongyong Feng, Yue Zhang, Yanfeng Chen et al.

The control region for mitochondrial DNA (mtDNA) has been considered as one of the most effective molecular markers in the study of identification for fish species, which has a fast evolutionary rate. And thus, in the present study, investigation of genetic co…

Prevalence and Chemotherapy of Cryptosporidiosis in Goats using Different Herbal and Allopathic Drugs in Southern Khyber Pakhtunkhwa, Pakistan

Naimat Ullah Khan, Muhammad Hassan Saleem, Aneela Zameer Durrani, Nisar Ahmad, Muhammad Shafee, Ayesha Hassan et al.

Cryptosporidium is a protozoan parasite causing diarrhea in human and animals. This study has been aimed to find out its prevalence and chemotherapy of cryptosporidiosis in goats in three selected districts of southern Khyber Pakhtunkhwa (KPK), Pakistan. A tot…

A New Approach for Evaluation of Egg Laying Behaviour of Alfalfa Weevil, Hypera postica (Gyllenhal) (Coleoptera: Curculionidae) during Autumn Using CHAID and MARS Algorithms

Celalettin Gözüaçık, Ecevit Eyduran, Mohammad Masood Tariq

The current study was conducted to develop a new approach for revealing egg-laying behaviour of alfalfa weevil, Hypera postica (Gyllenhal) (Coleoptera: Curculionidae)laying in alfalfa fields at late fall. Totally, the infected 675 alfalfa plants were evaluated…

Postmortem Findings in Captive Sand Gazelle and Arabian Oryx at Al-Wusta Wildlife Reserve, Oman

Khurram Goraya, Qais ALRawahi, Sultan ALBalushi, Hani ALSaadi, Sami ALRahbi, Zahir ALAlawi et al.

Al-Wusta Wildlife Reserve (WWR) is the first wildlife reserve in Oman. It was established in 1980 for reintroduction and breeding of the Arabian oryx (Oryx leucoryx) and recently other wildlife, which includes the Arabian gazelle (Gazelle gazelle cora) and San…

Red Onion Extract Attenuates Aluminum Induced Toxicity in Swiss Albino Mice

Chaman Ara, Asmatullah, Saima Hanif, Shagufta Andleeb, Beenish Zahid, Madeeha Arshad

This study was planned to investigate the therapeutic potential of onion extract against aluminum (Al) induced toxicity in male albino mice, Mus musculus. Eighty, 8-9 week old mice were divided randomly into eight groups, each containing 10 mice and exposed to…

Evolutionary Divergence of Signal Transducer and Activator of Transcription 5A (STAT5A) Gene in Riverine Buffalo

Muhammad Naveed, Asif Nadeem, Maryam Javed, Muhammad Fahad Bhutta, Ruqayya Bint Khalid

Signal transducer and activator of transcription 5A (STAT5A), also called mammary gland factor (MGF), is a key mediator of signal transduction within mammary gland and uterine epithelial cells. It is a member of placental lactogen (PL) and interferon-tau (IFN-…

The Arabian Camel, Camelus dromedarius Interferon Alpha: Cloning, Expression in Escherichia coli, in vitro Refolding and Cytotoxicity on Triple Negative Breast Cancer Cell Line MDA-MB-231

Hesham Saeed, Manal Abdel-Fattah, Ahmad Eldoksh, Farid S. Ataya, Manal Shalaby

Theopen reading frame encoding interferon alpha (IFNα) of the camel liver, Camelus dromedarius was isolated and cloned using reverse transcription-PCR. Sequence analysis of that gene showed a 564-bp encoding a protein of 187 amino acids with a predicted…

miR-22-3p Suppresses Adipocyte Formation of Telomerase Transformed Mesenchymal Stromal Cells (iMSC3) by Regulating Non-targeted MAPK Signaling Pathway

Sara Sultan Alomran, Muhammad Nasir Khan Khattak, Amir Ali Khan, Sallam Hasan Abdallah, Abeer Maher Fayyad, Khalid Bajou

Unraveling molecular mechanisms that govern adipocyte formation will lead to a greater understanding of obesity and subsequent treatment. In the current study, we assessed the effects of miR-22-3p, a non-coding RNA, on adipocyte differentiation from telomerase…

An In Vitro Assessment of the Cytotoxic and Apoptotic Potency of Silymarin and Silymarin Loaded Solid Lipid Nanoparticles on Lung and Breast Cancer Cells

Canan Vejselova Sezer

Cancer disease is a major cause of death worldwide in the last few decades. Recently, the risk of cancer and its mortality rates have increased both in economically less and highly developed countries. Two of the leading causes of the death of cancer are lung…

Investigation on the Effect of Two Fat Metabolism Related Pathways on Intramuscular Fat Content in Pigs

Chaogang Yao, Daxin Pang, Chao Lu, Aishi Xu, Peixuan Huang, Hongsheng Ouyang et al.

Intramuscular fat (IMF) content has become an important determinant of meat quality for raisers and consumers in modern society. The objective of this work was to investigate the roles of the peroxisome proliferator-activated receptor (PPAR) and fatty acid met…

Addition of L-Cysteine and Vitamin E to Semen Diluent Enhances Freeze-thawed Spermatozoa Characteristics in Crossbred Cattle Bulls under Subtropical Environment

Ibrar Muhammad Khan, Dezhi Xu, Zubing Cao, Hongyu Liu, Adnan Khan, Sajid Ur Rahman et al.

Vitamins and amino acids are important trace nutrients that are essential for body growth and reproductive soundness, whereas the lack of these nutrients is directly related to reproductive performance. Hence, the antioxidants agents(L-cysteine and vitamin E)…

Combined Effect of Aqueous Extracts of Artemisia monosperma and Mentha piperita on the Reproductive Integrity of Male Albino Rats

Hagar E. Mohammed, Ranwa A. Elrayess, Heba N. Gad El-Hak

The aim of this study was to evaluate the effect of combinations of Artemisia monospermia with Mentha piperita on male fertility.Treated rats with different doses of A. monosperma or M. piperita alone induced severe testes histopathological lesions and histoch…

Characterisation of Volatile Compounds and Composition of Milk of Kilis Goats Reared at Two Different Locations

Aylin Celile Oluk, Ugur Serbester, Murat Reis Akkaya, Oya Berkay Karaca

Various factors such as species, breeds, lactation stage and location affect milk composition. We evaluated the composition of Kilis goat milk in two different seasons on the basis of alcohols, ketones, aldehydes, esters, indoles, carboxylic acids, aromatic hy…

Polymorphisms of the CSN1S1 Gene and its Protein Variants in River and Swamp Buffalo (Bubalus bubalis)

Yongyun Zhang, Xinyang Fan, Fangting Zhou, Weizhen Li, Yina Ouyang, Yongwang Miao

Alpha S1-casein (αS1-CN) is a major casein in milk, which exerts a crucial role in casein transport and is related to individual milk components, nutritive value and production traits of milk. So far, αS1-CN coding gene (CSN1S1) has been widely stu…

Variance Analysis of Intestinal Microbial Diversity of the Noble Scallop (Chlamys nobilis) under Enrofloxacin Exposure

Jiawei Hong, Mingqiang Chen, Zhenghua Deng, Youning Li, Yu Wang

To investigate the possible impact of enrofloxacin (ENR) on noble scallop (Chlamys nobilis), we quantitatively evaluated the microbial shifts in the intestine of noble scallop in response to enrofloxacin treatments at different dosages (0, 5, and 10mg/L ENR) u…

Effect of Type of Degraded DNA Samples on Human Eye Color Prediction

Dilara Devranoglu, Ilksen Tavaci, Gonul Filoglu, Ozlem Bulbul

Single nucleotide polymorphisms (SNPs) are the most abundant genetic markers in the human genome. SNPs are used in forensics for predicting the externally visible characteristics of a given individual based on a sample of DNA alone. In this study, we tested de…

Length-Weight Relationships and Growth Patterns of Local Fishes of the Medium Perennial Vavuniya Reservoir, Sri Lanka

A.E.S. Patrick, S. Kuganathan, U. Edirisinghe

Linear regression analysis of length-weight relationships (LWRs) were studied to reveal the growth patterns of 380 specimens belonging to 10 species, 9 genera and 5 families of local fish in Vavuniya reservoir, Sri Lanka during January, 2013 to August, 2014. T…

Intervention Effect of Total Glucoside of Paeony on Mesangial Proliferative Glomerulonephritis in Rats

Xiujun Yao, Haofeng Wang, Ligong Zhang, Jingzhang Wu, Lijun Wang

Ten out of 30 rats were randomly selected as normal group. Remaining 20 rats were used to establish rat model of mesangial proliferative glomerulonephritis (MsPGN), and were equally divided into model group and total glucoside of paeony (TGP) group. TGP group…

Influence of Photoperiod and Shelter on Some Phenotypic Traits in African Catfish (Clarias gariepinus Burchell 1822) Larvae

Ahmed Saud Alsaqufi, Sheikh Mustafizur Rahman, Roshmon Thomas Mathew, Yousef Ahmed Alkhamis, Md. Moshiur Rahman, Muhammed Aslam Pathiri

Photoperiod and shelter have direct or indirect effects on phenotypic traits expression in different fish species. The present study was, therefore, intended to explore whether these light and shelter could influence some phenotypic traits of African catfish l…

B-ultrasonography: An Accurate and Noninvasive Method for Pregnancy Diagnosis in Pangolins

Fuhua Zhang, Yishuang Yu, Shibao Wu

Pregnancy diagnosis is critical to the management of captive breeding of wild animals, as accurate pregnancy diagnosis can improve the reproductive efficiency of captive animals. Pangolins do not show obvious morphological changes during pregnancy, which incre…

Expression of Prostaglandins-Related Genes in Erythrocytes of Chickens Infected with H9N2 Subtype of Avian Influenza Virus

Muhammad Farhan Qadir, Xin-Yu Han, Meng-li Qiao, Ying Wang, Ding Zhang, Yu-hai Bi et al.

Chicken’s erythrocytes are most abundant cells circulating in the body which participate in inflammation and immune responses. However, expression levels of prostaglandins-related genes in H9N2 infected chicken’s erythrocytes have not been studied…

In-vivo Evaluation of Anti-urolithiatic Activity of Different Extracts of Peel and Pulp of Cucumis melo L. in Mice Model of Kidney Stone Formation

Aneeqa Saleem, Muhammad Islam, Hamid Saeed, Mehwish Iqtedar

The aim of the present study was to investigate the in vivo anti-urolithiatic effects of both peel and pulp extractives of Cucumis melo L., in mice model of nephrolithiasis. Extractives of peel and pulp were obtained using hot and cold methods. Ethylene glycol…

Feasibility of Type D Botulinum Toxin for Rodent Prevention and Control in Plateau Pastoral Areas

Li Shengqing, Zhang Xiyun, Liu Shengcai, Hu Guoyuan, Fan Yuxia, Liu Huaixin et al.

This work aims to explore the feasibility of using type D botulinum toxin as a novel, environmentally friendly biological rodenticide for the population control of rodents in plateau pastoral area. The toxin is subjected to toxin component, DNA sequencing, and…

Wintering Behavior of Tundra Swan, Cygnus columbianus, at a Small Water-level Controlled Lake in the Middle and Lower Yangtze River Floodplain

Xinjian Wang, Eryan Yang, Lizhi Zhou

The Tundra swan, Cygnus columbianus, wintering in the lakes of Yangtze River floodplain are facing wetland degeneration and habitat loss. Daily activity rhythm, time budget and foraging pattern of wintering swans are the important presentation of behavioral st…

Genetic Diversity Analysis of Four Bactrian Camel Varieties in China

J.Y. Bai, R.T.D. Wu, Q. Zhang, D. Bao, L.M. Dao, X.H. Tian

In this study, 17 microsatellite markers were used to analyze the genetic polymorphism, genetic differentiation, gene flow and genetic distance of Alashan desert Bactrian camel, Alashan Gobi Bactrian camel, Sunite Bactrian camel and Qinghai Bactrian camel.The…

A New Species and a New Record of the Genus Stantonia Ashmead (Hymenoptera: Braconidae) from India and Saudi Arabia

Hamed A. Ghramh, Zubair Ahmed, Khalid Ali Khan

A new species viz., Stantonia hayati Ahmad, sp. nov., is described and illustrated from India, while S. hammersteini Enderlein, 1908is recorded from Saudi Arabia. Affinities of the new taxa with related species have also been discussed. The genus Stantonia is…

Antagonistic Probioticity of Novel Bacterial Isolates from Pakistan against Fish Pathogen Pseudomonas fluorescens in Labeo rohita Fingerlings

Asma Chaudhary, Qurat-ul-Ain Ahmad, Afia Muhammad Akram, Sadia Roshan, Javed Iqbal Qazi

The study was endeavoring to investigate in vitro as well as in vivo antibacterial and probiotic potential of Sphingomonas sp. AsCh-P3 (MF543123) and Bacillus aerius AsCh-A7 (MF543123) against fish pathogen Pseudomonas fluorescens. Antagonism by cross streak w…

Effects of Curcumin on Growth and Liver-protection in Common Carp, Cyprinus carpio

Yuanyuan Zhang, Liping Song, Hui Guo, Jun Wu, Xiaoli Wang, Fangbin Yao

We evaluated the growth performance and the hepatoprotective and antioxidant effects of curcumin against carbon tetrachloride (CCl4)-induced liver injury in common carp Cyprinus carpio. A 10-week feeding trial was carried out. A basal feed was supplemented wit…

Bioaccumulation of Selected Toxic Heavy Metals in Mastacembelus armatus from Three Rivers of Malakand Division, Pakistan

Hazrat Ali, Ezzat Khan

Contamination of water bodies with toxic heavy metals is a genuine environmental problem. Bioaccumulation of toxic heavy metals in fish poses a potential health risk to fish consumers including humans. The aim of present research work was to study the bioaccum…

Tenuisentis niloticus (Neoechinorhychida: Tenuisentidae) as Indicator of BTEX and PAH Pollution in Epe Lagoon, Lagos, Nigeria

BamideleAkinsanya, Isaac O Ayanda, Benson Onwusa, Joseph K Saliu

The Lagos lagoon is continually being subjected to all forms of pollution. Human activities like industrialization, agriculture, and oil exploration are constantly threatening the health of this aquatic ecosystem. Polycyclic aromatic hydrocarbons (PAH) and mon…

Effect of Duration of Diabetes Mellitus Type-2 on Electrophysiological Indices of Sensorimotor Nerves in Subjects with Diabetic Polyneuropathy: A Cross-Sectional Study

Ahsan Numan, Abdul Basit Qureshi, Fehmeda Farrukh Khan, Faisal Masud, Ijaz Ahmad, Kamran Ashraf et al.

The study investigated the effect of duration of diabetes mellitus type-2 on the electrophysiological characteristics of sensorimotor nerves in patients with clinical diabetic polyneuropathy (DSP). Clinical diagnosis of DSP was assessed with the Michigan Neuro…

Analysis of the Trend of Age-Related Laying Performance of Quail (Coturnix coturnix)

Jun Yan Bai, Zhi Hao Dong, Yu Chen, Jing Yun Li, Ying Lei, You Bing Yang et al.

For China yellow quail, Beijing white quail and Korean quail, number of eggs, total egg weight and laying rate begin to soar up with the increase of age from the 8th week to 12th week. Such growth rate declines gradually with the increase of age from the 12th…

Production of Protein Hydrolysate from Rohu (Labeo rohita) Waste using Fungal Proteases

Masood Sadiq Butt, Sadia Aslam, Rizwan Shukat, Syed Qamar Abbas, Muhammad Issa Khan, Shadab Shaukat et al.

Fish waste contains an appreciable amount of proteins, enzymes and fats. It was considered as garbage of no financial value and disposed of without any attempt of recovery. The aim of this study was to optimize the hydrolysis conditions for production of prote…

The Effect of miR-1468 on Growth Hormone in Pituitary Cells of Yanbian Yellow Cattle

Tai-hua Jin, An-gang Lou, Jiu-xiu Ji, Cheng-dou Cui, Long-zheng Yu2 andLi-zeng Guan

At present, the relationship between miRNAs and GH has been reported. However, the effect of miR-1468 on growth hormone (GH) is unknown. The objective of this study was to evaluate effects of miR-1468 on GH in pituitary cells of Yanbian yellow cattle in this s…

Remote Sensing Assessment of Marine Primary Productivity and Fishery Resources in the Daya Bay, China

Jing Yu, Jiangmei Mao, Dongliang Wang

Marine primary productivity (PP) is closely related with fishery resources. This study benefited a lot by providing the reference for the use of remote sensing data to inverse PP and then to assess fishery resources in typical offshore fishery waters. The pape…

Molecular Survey of Campylobacter jejuni in Broiler Chicken Farms in East Coast of Peninsular, Malaysia

Abdul Muhaimin Wahab, Basit Zeshan, Naveed Ahmed, Muhammad Afzal, Muhammad Naveed

Campylobacter spp. is responsible for food borne illness in humans while Campylobacter jejuni is the most common species for majority of human enteritis cases. The present study was conducted to determine the prevalence of C. jejuni, risk factors associated wi…

Antiviral Potential of Ivermectin against Peste des Petits Ruminants Virus (PPRV)

Saba Afzal, Sohail Raza, Masood Rabbani, Sehrish Firyal, Imran Altaf, Zahra Naeem

This study was conducted to find the antiviral potential of ivermectin against Peste des Petits Ruminants Virus (PPRV) in vitro. Firstly, cell cytotoxicity of ivermectin in the Vero cells was determined using MTT assay. The antiviral activity of ivermectin was…

Molecular Characterization and Pathogenesis of Local Isolates of Clostridium perfringens Type D from Sheep in District Peshawar

Saqib Nawaz, Sher Bahadar Khan, Umar Sadique, Muhammad Israr, Mumtaz Ali Khan, Hameed Ullah Khan et al.

The main objective of this study was isolation and determination of changes caused by Clostridium perfringens type D from sheep. For this purpose, a total of 272 fecal samples were collected from sheep and lambs from four different zones of district Peshawar.…

Effects of Adding Cysteine or Ascorbic Acid on the Quality of Frozen Semen from Arabian Stallions

Alamaary Mohaammed Saad, Abd Wahid Haron, Mohamed Ali, Mark Wen Han Hiew, Lawan Adamu

Spermatozoa are susceptible to the oxidative stress OS during cryopreservation. The excessive reactive oxygen species (ROS) and the capacity of the antioxidants could affect the OS level in the frozen semen. This study was to determine the addition of differen…

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A Review on Severe Acute Respiratory Syndrome 2 (SARS COV-2)

Arsalan Rasheed, Tahir Usman, Sadaf Niaz, Irfan Khattak, Saira Gul, Nawab Ali et al.

Coronavirus has attracted a lot of attention due to its ability to cause fatal zoonotic infections. These are the zoonotic viruses of both medical and veterinary importance. The virus spread through respiratory droplets and causes diseases, ranging from colds…

Plasmid Borne Antibiotic Resistance Factors in Indigenous Campylobacter spp. Isolated from Humans in Azad Jammu and Kashmir, Pakistan

Basharat Ahmed, Muhammad Hamza Azhar, Farah R. Shakoori, Abdul Rauf Shakoori

The antimicrobial susceptibility patterns of 146 Campylobacter spp.isolated from diarrheal patients admitted to hospitals in Azad Kashmir Pakistan were analyzed to determine their changing trends in response to fifteen antibiotics. Campylobacter isolates were…

Effect of Different Sonication Protocols on Merino Ram Sperm Parameters

Caner Öztürk, Mücahid Onay, Neşe Hayat Aksoy

 The biochemical analysis and the determination of the intracellular-extracellular contents of the sperm cells are important in the spermatological studies. Since homogenization processes reveal the intracellular contents of the sperm cells, we aimed to e…

Growth Response and Economics of Camelus dromedarius Calves Fed Isocaloric and Anisonitrogenous Rations

Asim Faraz, Abdul Waheed, Hafiz Muhammad Ishaq, Muhammad Shahid Nabeel

Marecha camel has good potential for commercial camel farming and it could be the source for future food production especially in arid and semi-arid areas of Pakistan. Research work on production potential of Marecha camel is scanty. In the current study, 10 m…