Issue · 2016
Vol.2, Iss. 1
4 articles · All issues →
CommentaryAutomated Aptamer Development May Represent the Last and Best Line of Defense against Proverbial
John G. Bruno
This commentary addresses the perceived need for automated robotic development of aptamers or aptamer conjugates with associated mass production to provide rapid passive immunity for large human populations in the event of a major pandemic or infectiou…
2 May 2016
Research ArticlePreliminary Development of an Enzyme-Linked Fluorescent DNA Aptamer-Magnetic Bead Sandwich Assay for Sensitive Detection of Rickettsia Cells
John G. Bruno, Chien-Chung Chao, Zhiwen Zhang, Wei-Mei Ching, Taylor Phillips, Allison Edge et al.
Abstract | DNA aptamers were developed against Rickettsia typhi (R. typhi) whole cells and individual candidate aptamer sequences were ranked according to affinity by an ELISA-like microplate-screening assay (ELASA). Top three candidate aptamers were then pair…
2 May 2016
ReviewWhole Cell SELEX Technology and its Applications
Neha Singh, Anjana Pandey
SELEX is an in vitro selection procedure to evolve single-stranded nucleic acids known as aptamers. Aptamers are known for their high selectivity and specificity and their ability to form 3D conformations. CELL SELEX is a modification of SELEX where inst…
30 Aug 2016
Research ArticleAptamer
John G. Bruno, Jeffery C. Sivils
Western blot-like analyses using DNA aptamers raised against the surfaces of general nonpathogenic Escherichia coli (ATCC No. 8739) as well as alpha and gamma intimins and Shiga-like toxin 2 (SLT-2) B subunit verified that the aptamers bound proteins of…
1 Sept 2016