Vol.49, Iss. 5

Effect of Pelleted and Chopped Wheat Straw on the Footpad Dermatitis in Broilers

Vladimir Avdalovic, Marijana Vucinic, Radmila Resanovic, Jelena Avdalovic, Danka Maslic-Strizak, Milos Vucicevic

An experiment was conducted to determine whether it is possible to replace the chopped wheat straw with newly formulated pelleted straw taking into account the prevalence and severity of footpad dermatitis (FPD) in broiler chickens. The study included 300 one-…

Comparative Study of Fatty Acid Composition of Gametes, Embryos and Larvae of Rainbow Trout, Oncorhynchus mykiss

Ayşe Gül Harlıoğlu

The fatty acid compositions of unfertilized eggs, semen, fertilized eggs, eyed embryo, yolk-sac larvae and absorbed yolk-sac larvae were investigated in rainbow trout(O. mykiss). The polyunsaturated fatty acids (PUFA) were found the highest followed by monouns…

Occurrence of Pedunculate Barnacles of the Symbiotic Genus Octolasmis (Cirripedia: Crustacea) in Two Species of Edible Crabs

Shazia Rasheed, Javed Mustaquim

In the present study, 5356 octolasmids hosted by two Portunus crabs, Potunus pelagicus and p. sanguinolentus, they belong to five species; namely Octolasmis angulata (Aurivillius, 1894), O. cor (Aurivillius, 1894), O. lowei (Darwin, 1851), O. tridens (Aurivill…

Some Reproductive Characteristics of Metapenaeus affinis (H. Milne Edwards, 1837) in Izmir Bay (Eastern Aegean Sea, Turkey)

Gülnur Metin, İlker Aydin

In this study, some reproductive characteristics (reproductive season, first maturation size (LM50) and sex ratio) of the jinga shrimp, Metapenaeus affinis (H. Milne Edwards, 1837), in Izmir Bay (Aegean Sea, Turkey) were investigated. A total of 2894 M. affini…

Optimization of Acridine Orange Staining for Buffalo Sperm, Cryopreserved in Egg Yolk based Extender to Detect DNA Fragmentation

Mushtaq Ahmad, Mehboob Ahmed, Nasim Ahmad

The integral genome of sperm is crucial for successful fertilization and embryonic development. Different techniques have been used to determine the integrity of DNA in which acridine orange (AO) assay is easy and less costly. Though used in many species but t…

Root Rot Disease Complex of Cotton: A Menace to Crop in Southern Punjab and its Mitigation through Antagonistic Fungi

Muhammad Arslan Khan, Sajid Aleem Khan, Imran-ul-haq, Rashad Waseem Khan

Root rot is very drastic disease prevalent in major cotton producing areas of Pakistan. Complex nature of disease due to root rot fungi and root-knot nematode cause huge losses to cotton. Experiments were conducted to assess the role of fungi and root knot nem…

Age, Growth, Length-Weight Relationship and Reproduction of Chub, Squalius cephalus (L., 1758) in Upper Akcay River, Turkey

Huseyin Sasi, Gulcin G. Ozay

For the first time, we investigate the aspect of bio-ecological of chub(Squalius cephalus L., 1758) in upper Akcay River (Buyuk Menderes Basin) including the age, growth, length-weight relationship and reproduction. The study was conducted in upper Akcay River…

Field Evaluation of Selective Systemic Formulations against Sucking Insect Pest Complex and their Natural Enemies on a Transgenic Bt Cotton

Talha Nazir, Muhammad Dildar Gogi, Muhammad Zeeshan Majeed, Waheed ul Hassan, Abdul Hanan, Muhammad Jalal Arif

Sucking insect pest complex, comprising of aphids Aphis gossypii Glover, jassids Amrasca devastans Distant, whiteflies Bemisia tabaci Gennadius, thrips Thrips tabaci Lindeman, red cotton bugs Dysdercus koenigii Walk and dusky cotton bugs Oxycarenus hyalinipenn…

Time Budget and Rhythm of Wintering Behaviors of Great Bustard in the Middle Reaches of Yellow River Basin of China

Wu Yi-qun, Xu Xiu

During the period from January 26th to February 3rd, 2013, the time budget and rhythm of wintering behaviors of Great Bustard (Otis tarda) was studied with instantaneous-scanning method in the intersection area of the Yellow River, the Wei River and the Luo Ri…

Evaluation of Resistance of Capsicum annum against Meloidogyne incognita and Sclerotium rolfsii and their Integrated Management

Adnan Yousaf, Jia Wu, Qaiser Shakeel, Yasir Iftikhar, Muhammad Irfan Ullah, Uzma Tahira et al.

Experiments were carried out to screen resistance of chili cultivars against Meloidogyne incognita and Sclerotium rolfsii and their integrated management by using derosol, cadusafos and Trichoderma harzianum in the green house at 25 ± 4ºC. Primaril…

Efficacy of Nuclear Polyhedrosis Virus and Flubendiamide Alone and in Combination against Spodoptera litura F.

Sumaira Maqsood, Muhammad Afzal, Muhammad Anjum Aqueel, Abu Bakar Muhammad Raza, Waqas Wakil2 andMuhammad Hussnain Babar

Nuclear polyhedrosis virus (NPV) is an important potential pathogen which can control a wide range of bollworm larvae in both agricultural and horticultural crpos. Flubendiamide is a new chemistry insecticide which also controls bollworm effectively and relati…

Construction and Characteristic Analysis of Omp10 Deletion Mutant of Brucella abortus

Tiansen Li, Meiling Huang, Zhen Wang, Fei Guo, Hui Zhang, Chuangfu Chen

Brucella is pathogenic bacteria that cause animal and human brucellosis. Currently, the mechanism behind the pathogenesis of Brucella remains unclear. For this reason omp10 mutant was constructed to examine the impacts of the outer membrane protein 10 (Omp10)…

Intestinal Parasitic Infections among Patients of Prince Sultan Military Medical City in Riyadh Region, Saudi Arabia: A 5-year Retrospective Study

Omar S.O. Amer, Mohamed I. Waly, andSaeed A. Al-Zahrani

This is a retrospective analysis of reported intestinal parasitic infections for patients visiting Prince Sultan Military Medical City, Riyadh, KSA from 2010 to 2014. Our retrospective study estimated a total 775 case out of 11110 case were infected with one o…

The Effect of Chemical and Surgical Castration on the Live Weight Gain and some Hormones of Male Norduz Sheep

Kadir Karakuş, Bahat Comba, Abuzer Taş, Tunahan Sancak, Arzu Comba, Devrim Saripinar Aksu et al.

The aim of this study was to investigate the effects of surgical and chemical castration on body weight and some hormones. In the present study, 2.5-3-month-old weaned male Norduz sheep were used. The sheep were obtained in the year 2015 from Livestock Applica…

Effect of Leaf Extracts of some Indigenous Plants on Settling and Oviposition Responses of Peach Fruit Fly, Bactrocera zonata (Diptera: Tephritidae)

Ayesha Ilyas, Hafiz Azhar Ali Khan, Abdul Qadir

The experiments were set up to investigate the settling and ovipositional deterrent effects of crude leaf extracts of six indigenous plants viz., amaltas (Cassia fistula), datura (Datura alba), neem (Azadirachta indica), niazboo (Ocimum basilicum), yellow kane…

Negative Association of HLA-DRB1*11 and HLA-DRB1*12 Alleles with Aeroallergy Patients Visiting Allergy Centre (NIH), Islamabad, Pakistan

Madiha Hashmi, Abid Hussain, Shafiq ur Rehman, Farida Ahmed, Shahbaz Aslam, Nadeem Afzal et al.

Aeroallergens include dust, pollens, cotton and threshing dust particles. Prevalence of different allergic diseases in Pakistan is about 66.1% and every year, almost 2.4 million people get vaccine for allergy from Allergy Centre, National Institute of Health (…

Association of Serum Resistin with Indices of Obesity in Young Pakistani Subjects

Farah Ashfaq, Tasnim Farasat

The aim of this research work was to examine the relationship of serum resistin with metabolic markers in obese Pakistani subjects. Anthropometric parameters including age, body mass index (BMI), waist circumference, waist-hip ratio (WHR), diastolic and systol…

Genotype and Phenotype Probabilities by Ternary Code based Line-dot Method and Polygonal Illustrations

Muhammad Imran Shabbir

The heart of learning genetics is in realizing that how the likelihood of inheriting a particular trait can be predicted. This fundamental approach is the basis for plant and animal breeding to get desired varieties as well as performing genetic analysis inclu…

Predicting Honey Production using Data Mining and Artificial Neural Network Algorithms in Apiculture

Koksal Karadas, Ibrahim Hakki Kadırhanogullari

This survey was conducted on all the 85 beekeeping farms collected with census study method in Igdir province of Turkey with the purpose of determining some factors influencing average honey yield (AHY) per beehive in the year 2014. For this purpose, predictiv…

Morphological Differences Between two Populations of the Little Tunny, Euthynnus alletteratus (Rafinesque, 1810) in Tunisian Waters (Central Mediterranean Sea)

Hassen Allaya, Abderraouf Ben Faleh, Manel Rebaya, Sonia Zrelli, Abdallah Hattour, Jean-Pierre Quignard et al.

In this study we analyzed possible differences among the specimens of the little tunny Euthynnus alletteratus (Rafinesque, 1810) inhabiting Tunisian waters. Our investigation based on the analysis of morphological characteristics of Euthynnus alletteratus from…

Effect of Wheat and Corn Bran and Barley and Sorghum β-Glucan Extracts on the Plasma Cholesterol Level of Dietary-Induced Hypercholesterolemic Rats

A. Hamid, M. Ilyas, S. Kalsoom

Hypercholestrolemia leading to cardiovascular diseases are very common problem in Pakistan and in most cases it is caused due to insufficient intake of dietary fiber.A study was conducted to evaluate the role of dietary fiber and ß-glucan from major food…

Antimicrobial and Anti-Prostate Cancer Activity of Turmeric (Curcuma longa L.) and Black Pepper (Piper nigrum L.) used in Typical Pakistani Cuisine

Saba Irshad, Ayesha Ashfaq, Ammara Muazzam, Abida Yasmeen

Plant extracts have been used as an active antimicrobial compound since long. The present study was intended to validate the antimicrobial, antidiarrheal and anticancer activity of turmeric (Curcuma longa L.) and black pepper (Piper nigrum L.), used in Pakista…

Molecular Cloning and Expression of Taste Receptor Gene T2R1 of Obscure Puffer, Takifugu fasciatus

Yijin He, Song Ma, Bo Liu, Ting Xue, Qunlan Zhou, Wu Jin et al.

Obscure puffer, Takifugu fasciatus is a unique species of China which is distributed in the estuary of the Yangtze River, and belongs to the same genus with Red fin puffer T. rubripes which has been used as one of human genome model animal since mid-1990s. In…

Molecular Phylogeny and Diversity Analysis of Hog Deer (Axis porcinus) in Pakistan

Ghulam Abbas, Asif Nadeem, Masroor Ellahi Babar, Tanveer Hussain, Muhammad Sajid Tahir, Wasim Shehzad et al.

Many species of mammals have declined within the past two centuries due to human caused disturbances and the unsustainable use of natural resources. Molecular methods have an important role in phylogeny and diversity analysis. In Pakistan hog deer (Axis porcin…

Growth Performance and Nutrient Digestibility of Cirrhinus mrigala Fingerlings Fed Phytase Supplemented Sunflower Meal Based Diet

Syed Makhdoom Hussain, Tasneem Hameed, Muhammad Afzal, Arshad Javid, Nosheen Aslam, Syed Zakir Hussain Shah et al.

A feeding trial was conducted to study the effects of graded levels (0, 500, 1000, 1500 and 2000 FTU kg-1) of microbial phytase on growth performance and nutrient digestibilityof Cirrhinus mrigala fingerlings fed sunflower meal based diet. The chromic oxide wa…

Estimation of Cold Carcass Weight and Body Weight from Several Body Measurements in Sheep through Various Data Mining Algorithms

Ali Karabacak, Senol Celik, Adile Tatliyer, Ismail Keskin, Yakup Erdal Erturk, Ecevit Eyduran et al.

The goal of the present study was to compare the predictive performance of three data mining algorithms viz., CHAID, Exhaustive CHAID, and CART implemented in the estimation of cold carcass weight (CCW) and body weight (BW) from several body measurements (with…

Spatial Distribution of Soil Borne Brucella Species Specific DNA in Punjab, Pakistan

Rais Ahmed, Khushi Muhammad, Masood Rabbani, Muhammad Sarwar Khan

Brucella species are cause of abortions and other reproductive disorders in animals and human beings. In this project, prevalence and distribution of soil borne Brucella species in nine districts of Punjab including Lahore, Faisalabad, Sheikhupura, Sargodha, D…

Phylogenetic Relationship of Locally Isolated Paramecium Species Inferred from Histone H4 Genes

Fareeda Tasneem, Farah Rauf Shakoori, Abdul Rauf Shakoori

The histone H4 gene is supposed to be highly conserved among all organisms with the exception of variations found in the ciliate species. Thus a fragment of histone H4 gene (160bp) was sequenced in ten locally isolated strains of Paramecium species including a…

Influence of Feeding Rate on the Growth, Feed Efficiency and Carcass Composition of the Giant Gourami (Osphronemus goramy)

Netti Aryani, Azrita, Ainul Mardiah, Hafrijal Syandri

Aquaculture feeding rate is an important factor affecting the growth of giant gourami. The aims of the study were to investigate the effect of different feeding rates on the growth, and carcass composition of giant gourami (initial weight 14.17±0.15 g a…

A Recurrent Mutation in CTSK Gene is Responsible for Autosomal Recessive Pycnodysostosis in Consanguineous Pakistani Families

Mehran Kausar, Naveed Ashraf, Farzana Hayat, Asraf Hussain Hashmi, Saima Siddiqi, Mariam Anees

Pycnodysostosis is a rare genetic bone disorder (OMIM 265800) with an autosomal recessive mode of inheritance. More than 50 mutations have been reported in CTSK (Cathepsin k) responsible for this disease. Mutations in CTSK result in impaired bone resorption co…

Bioaccumulation of Heavy Metals by Metal-Resistant Bacteria Isolated from Tagetes minuta Rhizosphere, Growing in Soil Adjoining Automobile Workshops

Kulsoom Akhter, Tahseen Ghous, Saiqa Andleeb, Faiz-ul-Hassan Nasim, Samina Ejaz, Zain-ul-Abdin et al.

The present study deals with isolation, identification and characterization of some indigenous heavy metal resistant bacteria isolated from rhizosphere of Tagetes minuta, growing in soil adjoining automobile workshops to investigate their bioaccumulation capac…

Rumen Discoloration of Growing Lambs Fed with Diets Containing Different Levels of Neutral Detergent Fibre

Ibrahim A. Alhidary, Mutassim M. Abdelrahman, Riyadh S. Aljumaah, Abdullah H. Alyemni, Moez A. Ayadi, Mohamed Y. Al-Saiady

A study was conducted to investigate the effects of different total mixed ration diets (TMRs) with three different levels of NDF on growth performance, metabolic profile and rumen characteristics in Naemi lambs. Fifty growing Naemi lambs at age of three months…

Effect of Betaine Supplementation on the Performance and Immune Response of Heat Stressed Broilers

Naila Chand, Shabana Naz, Hamza Maris, Rifat Ullhah Khan, Sarzamin Khan, Muhammad Subhan Qureshi

This study was planned to investigate the effects of betaine supplementation on growth performance and immunity in broilers under natural summer stress. A total of one hundred and twenty day-old broiler chicks were divided into four different treatment groups…

New Genetic Evidence from Three Keel-Backed Liza Species Based on DNA Barcoding Confirms Morphology-Based Identification

Lu Liu, Sher Khan Panhwar, Tianxiang Gao, Zhiqiang Han, Chunhou Li, Dianrong Sun et al.

Three Liza species exhibiting a dorsal keel have been identified based on morphology: Liza affinis, Liza carinata, and Liza klunzingeri. To confirm the validity of the species by molecular methods, we sequenced a fragment of the cytochrome oxidase subunit I (C…

Isolation and Characterization of Microsatellite Markers for the Sable, Martes zibellina (Mammalia: Mustelidae)

Wanchao Zhu, Qinguo Wei, Shuyu Xue, Huanxin Zhang, Tianshu Lv, Honghai Zhang

The number of wild sables has declined dramatically in recent years due to the high value and increasing demand of their fur. In order to evaluate the genetic diversity and population structure for the conservation and management of sables (Martes zibellina),…

Organization and Variation of the Mitochondrial DNA Control Region in Ardeidae (Aves: Ciconiiformes) and their Phylogenetic Relationship

Zuhao Huang, Meifang Li, Rujue Ruan, Jiawei Qin

Control region is a non-coding region of mitochondrial genome. To infer the organization and variation of Ardeidae mitochondrial DNA control region, the control region genes of 20 species were analyzed. All the Ardeidae species had duplicate control regions, e…

Association between the COMT Gene and Obsessive Compulsive Disorder: A Case-Control Study

Javeria, Masroor Ellahi Babar, Akhtar Ali, Asif Nadeem, Abdul Wajid, Sajjad Ali Shah et al.

This studyaimedto find out the genetic variations in Catechol O Methyl transferase (COMT) gene and association of these genes with obsessive compulsive disorder (OCD) in the Pakistani Patients. We selected OCD patients (n=100) following the Diagnostic Statisti…

Evaluating Varying Calf Milk Replacers for Optimum Growth Performance in Salt Range Lambs

Muhammad Fiaz, Khadija Munawar, Muhammad Mushtaq, Mukhtar Ahmad, Mushtaq Ahmad Chaudhry, Muhammad Aslam et al.

The objective of study was to evaluate through feeding calf milk replacer to lambs for optimum growth performance and survivability. For this purpose, Salt Range neonatal lambs (n = 24, age = 01 week) were selected and divided equally into three groups having…

Association of Gly482Ser Varient of PPARGC1α Gene with Diabetes in Pakistani Population

Shabana, Farah Ehsan, Shahida Hasnain

The aim of this study was to determine the association of Gly482Ser variant of proliferator activator receptor γ coactivator 1α (PPARGC1α) gene with diabetes in Pakistani population. A total of 900 blood samples (diabetic=451, controls=449) w…

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We collected Platycephalus indicus individuals from Beihai and accurately described the species. The diacritically meristic counts were listed as follows: first dorsal fin with a single small isolated spine anteriorly; gill rakers 2-3+4-6=6-9; pored lateral li…

Behavioural Rhythms during the Adaptive Phase of Introduced Milu/Pere David's Deer, Elaphurus davidianus, in the Dongting Lake Wetland, China

Shuangye Wang, Yunlin Zhao, Zhenggang Xu, Li Li, Liang Wu, Choucang Duan et al.

To explore the adaptation of introduced Milu(Elaphurus davidianus), also known as Père David’s Deer, to their new environment, this study was designed to track and observe 16 Milu, which were released in the Dongting Lake wetland area from Jiangsu…

Table of Contents

Detection of Insecticide Residues in Honey of Apis dorsata F. from Southern Punjab, Pakistan

Muhammad Aslam Farooqi, Mansoor-ul-Hasan, Sohail Akhtar, Muhammad Arshad, Muhammad Naveed Aslam, Muhammad Rafay

A simple and fast analytical method using High Performance Liquid Chromatography with ultraviolet detection (HPLC-UV) was used to determine residues of commonly used agricultural insecticides (imidacloprid, thiametoxam, profenofos, endosulfan, spinosad and del…

Bioaccumulation of Cu and Zn in Schizothorax plagiostomus and Mastacembelus armatus from River Swat, River Panjkora and River Barandu in Malakand Division, Pakistan

Hazrat Ali, Wajid Ali, Karim Ullah, Fazle Akbar, Sher Ahrar, Irfan Ullah et al.

Contamination of aquatic ecosystems with heavy metals leads to accumulation of these elements in aquatic biota including fish. Bioaccumulation of heavy metals in fish is of public health concern because fish are an important part of human diet. The present stu…

Epidemiology of HIV/AIDS and Syphilis among High Risk Groups in Pakistan

Usman Waheed, Humayoon Shafique Satti, Muhammad Arshad, Ahmed Farooq, Abdul Rauf, Hasan Abbas Zaheer

The prevalence of HIV/AIDS and syphilis is rapidly increasing among high risk groups (HRGs) in Pakistan leading it away from achieving the 6th millennium development goal of halting and reversing HIV/AIDS epidemic. Syphilis increases the risk of HIV transmissi…

Evaluation of the Growth Performance, Body Composition and Survival Rate of Juvenile Snakehead (Channa marulius) Fed on Different Feeds

Hameeda Kalhoro, Abdul Malik, Ghulam Abbas, Illahi Bux Kalhoro, Sajjad Ali Shah4and Haleema Kalhoro

This research was done to determine the influence of various feeds on growth increment, body constituents and survival rate of snakehead (Channa marulius) cultured in plastic tanks (42.5 liters water carrying capacity each). Fish juveniles (length: 10.63 &plus…

Assessment of Feeding Value of Processed Rice Husk for Lohi Sheep in Growing Phase

Rahat Naseer, Abu Saeed Hashmi, Zulfiqar-ul-Hassan, H. Rehman, Saima Naveed, F. Masood et al.

The present project was envisaged by using rice husk as a substrate for processing with the objective to produce a nutritionally enriched bulk feed ingredient and to explore its potential as wheat bran replacer in total mixed rations for the ruminants. For thi…

Biodiversity and Species Distribution of Coccinellids (Coccinellidae: Coleoptera) in District Sargodha (Punjab), Pakistan

Kanwer Shahzad Ahmed, Muhammad Zeeshan Majeed, Muhammad Ather Rafi, Fatima Sellami, Muhammad Afzal

Coccinellid beetles (Coccinellidae; Coleoptera) are voracious predators of many insect pests of economic importance. Among 6,000 species of coccinellids described worldwide, only 75 species have been reported from Pakistan so far. Keeping in view the limited w…

Histological and Ultrastructural Features of the Leydig Cells and their Association with other Testicular Cells of the Vervet Monkey, Chlorocebus aethiops

Sogolo L. Lebelo, Gerhard van der Horst

The study was undertaken to investigate the structure of the Leydig cells of the vervet monkey, Chlorocebus aethiops, using both light and electron microscopic techniques and to elucidate the association of these cells with other testicular cells. The Leydig c…

New Hipparionine (Perissodactyla) Fossils from Potwar Plateau of Pakistan

Muhammad Hanif, Naureen Rana, Muhammad Akbar Khan, Muhammad Javed, Muhammad Sajjad Khan

New dental material of hipparionines from the Middle Siwalik deposits of district Attock, Potwar Plateau, Punjab, Pakistan has been described. The fossiliferous sites, Dhok Mila of Nagri Formation (early Late Miocene) and Kaulial Kas of Dhok Pathan Formation (…

Habitat Ecology and Breeding Performance of Cattle Egret (Bubulcus ibis) in Faisalabad, Pakistan

Muhammad Abdullah, Rashid A. khan, Muhammad Rafay, Tanveer Hussain, Tahira Ruby, Fariha Rehman et al.

Cattle egret is considered as biological insect pest control agent in our agricultural ecosystem. To analyze this activity present work was planned to collect the preliminary information about habitat requirements and different breeding aspects of Cattle egret…