Articles
3,478 open-access articles · page 130 of 174
Research ArticleDevelopment and Characterization of Microsatellite Markers for the Ornamented Pygmy Frog Microhyla fissipes and Transferability in Microhyla
Lusha Liu, Lei Cheng, Xingqi Zhang, Yulong Li, Jianping Jiang
Microhyla fissipes, from family Microhylidae suborder Neobatrachia, widely distributes from western Myanmar eastward through Indochina and northward into southern China including Hainan and Taiwan. But the availability of microsatellites especially from expres…
12 Jul 2019DOI 10.17582/journal.pjz/2019.51.5.1881.1889
Research ArticleSequencing Analysis of the Mitochondrial Genome of Japanese Sand Lance Ammodytes personatus Based on Next-Generation Sequencing Technology
Jingchen Chen, Zhaochao Deng, Zhiqiang Han
The next-generation sequencing technology is obviously faster than Sanger sequencing technology, and the cost is basically the same. Based on this, we used the next-generation sequencing technology to measure the whole mitochondrial genome sequence of Ammodyte…
12 Jul 2019DOI 10.17582/journal.pjz/2019.51.5.1869.1880
Research ArticleOsteological Development of the Vertebral Column and Caudal Complex in Larval and Juvenile Blackhead Seabream, Acanthopagrus schlegelii (Perciformes, Sparidae) (Bleeker, 1854)
Jing Hu, Yan Liu, Gang Yu, Changping Yang, Binbin Shan, Shengnan Liu et al.
Osteological development of the vertebral column and caudal complex in blackhead seabream was described under extensive larval rearing conditions from 1DPH to 36DPH. The development of vertebral column originated from the occurrence of neural arch and hypural…
12 Jul 2019DOI 10.17582/journal.pjz/2019.51.5.1859.1867
Research ArticleIsolation and Characterization of Microsatellite Loci from an Ice Fish, Neosalanx tangkahkeii (Osmeriformes, Salangidae)
Jie Zhang, Baradi Waryani, Qihai Zhou
The icefish Neosalanx tangkahkeii is an endemic species in East Asia. It has a wide range of distribution in Chinese waters including coastal waters, inland lakes, and outflowing rivers south of the Yangtze River and its delta. In order to maintain stable…
12 Jul 2019DOI 10.17582/journal.pjz/2019.51.5.1853.1857
Research ArticlePathogenic Potential of Javanese Root-knot Nematode on Susceptible and Resistant Okra Cultivars
Tariq Mukhtar, Muhammad Arshad Hussain
The Javanese root-knot nematode (Meloidogyne javanica)is becoming a serious threat to okra production in Pakistan. The damaging potential of this nematode has not been studied on resistant and susceptible okra cultivars, therefore, in the present study, the ef…
12 Jul 2019DOI 10.17582/journal.pjz/2019.51.5.1891.1897- Research Article
Effect of Boron on the Potassium Dichromate Induced Oxidative Damage in Brain Tissue of Sprague Dawley Rats
Zeynep Soyer Sarıca, Meryem Eren, Meryem Senturk
In this study the effect of the trace mineral, boron (B) was determined on the potassium dichromate induced oxidative damage in the brain tissue of Sprague Dawley rats. Malondialdehyde (MDA) levels and activities of superoxide dismutase (SOD), catalase (CAT) a…
12 Jul 2019DOI 10.17582/journal.pjz/2019.51.5.1905.1910 
Research ArticleExpression Analysis of Oxidative Stress Induced Genes in Liver and Heart Tissues in Response to Doxorubicin
Uzma Jabeen, Irfan Khan, Nadia Naeem, Asmat Salim, Waseem Ahmed
Drugs used in chemotherapy that target rapidly dividing cells, also exert toxic effects on healthy tissues. Doxorubicin (DOX) is a commonly used chemotherapeutic drug. Oxidative stress plays an important role in DOX-induced toxicity. This study was aimed at an…
12 Jul 2019DOI 10.17582/journal.pjz/2019.51.5.1899.1903
Research ArticleDevelopment and Clinical Evaluation of a Direct Amplification Method to Diagnose Canine Parvovirus and Canine Distemper Viral Infections in Dogs without Nucleic Acid Extraction
Xuefeng Cao, Guangneng Peng, Xiaobin Gu, Changliang He, Guizhou Yue, Jun Shi et al.
To rapidly detect canine parvoviruses (CPV) or canine distemper virus (CDV) without nuclear acid extraction, we established a direct amplification TaqMan real-time (RT)-PCR method (DARPM) to detect CPV and CDV in clinical samples. We compared this new method a…
5 Jul 2019DOI 10.17582/journal.pjz/2019.51.5.1843.1852
Research ArticleComparative Evaluation of Live Vector, Immune Complex and Intermediate Plus Vaccines of Infectious Bursal Disease in Broiler Chicken
Rana Waqas Arshad, Asim Aslam, Muhammad Saeed Imran, Kamran Ashraf, Raheela Akhtar
The aim of the present study was to compare commercially available vaccines of infectious bursal disease (IBD). Three experimental groups, each having 100 broiler birds were placed in the same environmentally controlled house. Samples of blood and bursa of Fab…
5 Jul 2019DOI 10.17582/journal.pjz/2019.51.5.1837.1841
Research ArticleBiodiversity and Abundance of Plankton Communities along the Coastal Waters of Gulf of Aqaba, Saudi Arabia
Mohsen M. El-Sherbiny, Reny P. Devassy, Erinn M. Muller, Abdulmohsin A. Al-Sofyani, Ali M. Al-Aidaroos
Spatial variation in the microphytoplankton biomass and diversity as well as the diel variability of the major mesozooplankton taxon, Copepoda was studied with respect to the prevailing hydrographic conditions along the coastal waters of Gulf of Aqaba, Saudi A…
5 Jul 2019DOI 10.17582/journal.pjz/2019.51.5.1823.1836- Short Communication
Comparative Analysis of Immunological and Genomic Outcomes of Dengue Virus Outbreak in Pakistan
Muhammad Shahid, Iram Amin, Samia Afzal, Zareen Fatima, Muhammad Idrees
The mortality and morbidity due to dengue fever is increasing worldwide at a frightful rate. Emergence and re-emergence of dengue fever in previous epidemics has been documented globally, including Pakistan. We conducted a comprehensive study and compared the…
27 Jun 2019DOI 10.17582/journal.pjz/2019.51.5.sc4 
Short CommunicationGenetic Diversity and Population Structure of Seven Tibet Yak Ecotype Populations using Microsatellite Markers
Yan-Bin Zhu, Wang-Dui Basang, Zhan-Dui Pingcuo, Yang-Ji Cidan, Sang Luo, Dun-Zhu Luosang et al.
The aim of the present study was to perform genetic diversity and population structure estimation on 382 individuals from seven Chinese Tibetan ecotype yak population using twenty-one microsatellites. The results revealed that the HO ranged from 0.4854±…
27 Jun 2019DOI 10.17582/journal.pjz/2019.51.5.sc6
Research ArticleIn-vitro Toxicity Evaluation of some Phytoextracts against Mealybug Drosicha mangiferae (Hemiptera: Pseudococcidae) Infesting Citrus Orchards in Pakistan
Hafiz Abdul Ghafoor, Muhammad Afzal, Muhammad Asam Riaz, Muhammad Zeeshan Majeed
Mealybug Drosicha mangiferae (Pseudococcidae: Hemiptera) is one of the destructive insect pests of many agricultural and horticultural crops including citrus. Extensive and injudicious use of conventional synthetic insecticides against D. mangiferae have led t…
27 Jun 2019DOI 10.17582/journal.pjz/2019.51.5.1815.1822
Research ArticleMicrobial Diversity in the Gastrointestinal Tract of a Bat, Hypsugo alaschanicus
Zhimin Yuan, Yan Yu, Yanmei Wang, Yanzhen Bu, Hongxing Niu
Bats are a potential reservoir of zoonotic pathogens. Some strains of bacteria in the gastrointestinal tract of bats are pathogenic to humans. Hypsugo alaschanicus feeds on insects and has a wide geographic distribution in China, and people are in frequent con…
27 Jun 2019DOI 10.17582/journal.pjz/2019.51.5.1807.1813- Short Communication
Prevalence of Plant Nematodes Associated with Maize in Balochistan, Pakistan
Aly Khan, Khalil A. Khanzada, S. Shahid Shaukat
A survey of maize (Zea mays L.) of eight localities, namely Anjira, Kalat, Khazeena, Khuzdar, Mangochar, Rodeni, Surab and Zehri was conducted in Balochistan. A total of 13 plant nematodes were recorded. Out of these seven nematodes were encountered in only on…
27 Jun 2019DOI 10.17582/journal.pjz/2019.51.5.sc5 
Research ArticleCysticidal Activity of Trachyspermum ammi Essential Oil against Acanthamoeba Isolates from Dental Clinic and Hospital Water Networks
Wafaa M. Hikal, Hussein A.H. Said-Al Ahl
Acanthamoeba genus is widespread in the environment, including the hospital water sources and medical devices especially in dental units and dialysis machines. It poses a threat to public health as it is responsible for human infection. In inappropriate condit…
24 Jun 2019DOI 10.17582/journal.pjz/2019.51.5.1799.1806- Research Article
Dietary Supplementation of Fenugreek (Trigonella foenum graecum) on the Egg Quality Characteristics of Rhode Island Red Spent Layers
Fazal Wahab, Naila Chand, Rifat Ullah Khan, Nazir Ahmad, Urooba Parvez, Zia ur Rehman et al.
The present research was conducted to investigate the effect of various levels of fenugreek seeds supplementation on feed intake, egg production and egg quality of Rhode Island Red spent layers. A total of 60 spent Rhode Island Red layers were assigned to 4 tr…
24 Jun 2019DOI 10.17582/journal.pjz/2019.51.5.1793.1797 
Research ArticleRole of Salicylidene Acylhydrazide and Proteases in Biofilm Inhibition of Desulfovibrio spp.
Iram Liaqat, Nazish Mazhar Ali, Najma Arshad, Riffat Iqbal, Zain-ul-Abideen
This study has highlighted that several compounds including salicylidene acylhydrazide, proteinase k, trypsin and chymotrypsin target bacterial virulence in Desulfovibrio spp. that causes bacteremia, periodontitis and abdominal infections. Ten soil samples wer…
24 Jun 2019DOI 10.17582/journal.pjz/2019.51.5.1783.1791- Research Article
Effects of Vitamin C and Vitamin E on Cryopreservation of Guanzhong Donkey Semen
Xiaohui Yu, Shuai He, Liqiang Wang, Mengyang Kang, Yanjiao Zhu, Shuhui Wang et al.
Sperm cryopreservation offers an effective method to protect Guanzhong donkey, a precious species. The objective of this study was to reveal the effect of different antioxidants on the semen cryopreservation in Guangzhong donkey by testing indices of sperm mot…
24 Jun 2019DOI 10.17582/journal.pjz/2019.51.5.1777.1781 
Research ArticleAssociation of Lifestyle and Physical Activity with the Components of Metabolic Syndrome - A Study of Bank Employees in Lahore, Pakistan
Rabia Khalid, Saleema Bashir Shams, Bibi Nazia Murtaza, Gaitee Joshua, Saira Mushtaq, Hassan Al-Talhi et al.
Metabolic syndrome (MetS) leads to millions of preventable cases and deaths worldwide.Present study reveals the components of metabolic syndrome among the bank employees in Lahore area of Pakistan. Total 530 male individuals were recruited in the study. Associ…
24 Jun 2019DOI 10.17582/journal.pjz/2019.51.5.1761.1768