Articles
3,478 open-access articles · page 134 of 174
Research ArticleDevelopment and Validation of a Transcriptome-Based Simple Sequence Repeats Markers in Coilia nasus
Fukuan Du, Yongkai Tang, Juhua Yu, Shengyan Su, Fan Yu, Jianlin Li et al.
Estuarine tapertail anchovy (Coilia nasus) is a commercially important species. However, eco-environment deterioration and overfishing have almost caused extinction of the species in the Yangtze River. Therefore, it is very urgent to perform genetic research o…
2 May 2019DOI 10.17582/journal.pjz/2019.51.4.1335.1341
Research ArticleIsolation and Biological Characterization of Muscle-Derived Stem Cells from Sheep Skeletal Muscle
Ping Zhang, Yabin Pu, Yu Zhang, Jia Chen, Kunfu Wang, Qian Li et al.
The Objective of this study was to investigate the isolation, cultivation, identification and differentiation potential of Muscle-derived stem cells (MDSCs) from sheep skeletal muscle and provide an experimental evidence for clinical application. The skeletal…
1 May 2019DOI 10.17582/journal.pjz/2019.51.4.1259.1272
Research ArticleAnalysis of the Phylogeny and Evolutionary Selection Pressure of the Mx Gene in 10 Wild Birds
SiRui Wang, Fekede Regasa Joka, XiaoLong Wang, SuYing Bai
The Myxovirus-resistance (Mx) gene has broad-spectrum antiviral effects. This gene is particularly important for the prevention and treatment of avian influenza. Currently, this gene is more studied in poultry, and studies in wild birds are rare. To understand…
1 May 2019DOI 10.17582/journal.pjz/2019.51.4.1299.1307
Research ArticlePopulation Genetic Structure and Genetic Diversity of Coral Reef Species Lethrinus olivaceus in the South China Sea
Zhaochao Deng, Na Song, Yongzhen Li, Tianxiang Gao, Zhiqiang Han
Lethrinus olivaceus is an important coral reef fish in the South China Sea. To reveal the genetic structure and genetic diversity of L. olivaceus, a 495 bp segment of mitochondrial DNA control region was sequenced. We collected a total of 65 individuals from t…
1 May 2019DOI 10.17582/journal.pjz/2019.51.4.1289.1297
Research ArticleNew and Known Nematodes Associated with Cotton Plantation in Sindh, Pakistan
Ashfaque Ahmed Nahiyoon, Shahina Fayyaz, Nasira Kazi
Cotton is a major crop of Pakistan after wheat and it occupies the largest area in Pakistan compared to other crops. Cotton is cultivated in Sindh on more than 1 million acres. Two popular varieties of cotton are grown in Sindh. The aim of the present study wa…
1 May 2019DOI 10.17582/journal.pjz/2019.51.4.1309.1314
Research ArticleEffect of Different Diapause Conditions on Bombus terrestris Colony Characteristics
Muhammad Nasir, Ata-ul-Mohsan, Shafqat Saeed, Munir Ahmad, Muhammad Asif Aziz2 andMunir Ahmad
Diapause is the very important stage in the artificial rearing of bumblebees that entails costs that become visible after diapause completion. In the present study different diapause temperature (-2-0 ºC), (1-3 ºC), (4-6 ºC) and duration (two an…
1 May 2019DOI 10.17582/journal.pjz/2019.51.4.1273.1280
Research ArticleGenetic Homogeneity among Bull Sharks Carcharhinus leucas in the South China Sea
Zhaochao Deng, Jingchen Chen, Na Song, Yongzhen Li, Zhiqiang Han
Carcharhinus leucas has a cosmopolitan distribution and marked genetic structure. However, analysis of this structure has not included the South China Sea population. In the present study, we collected a total of 23 individuals from Xisha, and Nansha Islands i…
1 May 2019DOI 10.17582/journal.pjz/2019.51.4.1281.1288
Research ArticleCharacterization of Thermophilic Bacteria Anoxybacillus rupiensis and Cultivation in Agroindustrial Wastes Isolated from Hot Spring in Chakwal, Pakistan
Faiza Jabeen, Bushra Muneer, Javed Iqbal Qazi
Two thermophilic bacteria were isolated from hot spring located near the city Chakwal in Pakistan. The cells of both strains were rod-shaped, stained Gram positive and formed endospores. The isolates were able to utilize sugars like fructose, Maltose, mannose,…
30 Apr 2019DOI 10.17582/journal.pjz/2019.51.4.1243.1250
Research ArticleExtended-Spectrum-β-Lactamase Producing Multidrug Resistant Klebsiella pneumoniae Isolates from Pediatrics
Sumreen Hayat, Muhammad Hussnain Siddique, Bilal Aslam, Habibullah Nadeem, Asma Ashraf, Muhammad Saqalein et al.
Klebsiella pneumoniae is mainly responsible for nosocomial and urinary tract infections (UTIs) in pediatric and adult population. The ESBL producing K. pneumoniae present an alarming situation for clinicians by limiting the treatment options. We aimed to deter…
30 Apr 2019DOI 10.17582/journal.pjz/2019.51.4.1251.1257
Research ArticleStudies on the Recombinant Production in E. coli and Characterization of Pharmaceutically Important Thermostable L-Asparaginase from Geobacillus thermodenitrificans
Muhammad Shahid Nadeem, Maryam A. Al-Ghamdi, Jalaluddin Azam Khan
L-asparaginase is ubiquitously found in microbes, animals and plants. The asparaginase of bacterial origin has been extensively applied in the treatment of childhood lymphomas and leukaemia. However, the glutaminase activity of enzyme can cause serious side-ef…
30 Apr 2019DOI 10.17582/journal.pjz/2019.51.4.1235.1241
Research ArticleConformational Changes in Wild Type KRAS Induced by Two Novel Variants p.E31K and p.G138V
Bibi Nazia Murtaza, Azhar Qayum, Shamaila Inayat Nadeem, Naif Awdh Al-Maliki, Abdulaziz Alamri, Abdul Rauf Shakoori
Ras proto-oncogene encodes for small GTPases, downstream of epidermal growth factor receptor (EGFR) in the RAS/RAF/MAPK pathway. Wild type KRAS is associated with EGFR-signalling activation. In normal physiological conditions, it is activated by upstream signa…
29 Apr 2019DOI 10.17582/journal.pjz/2019.51.4.1227.1233
Research ArticleEffect of High Vitamin E Dosages on Lipid Peroxidation and Fatty Acid Profile of Labeo rohita Fingerlings
Mahroze Fatima, Muhammad Afzal, Syed Zakir Hussain Shah
A 60 days feeding trial was conducted to investigate the effect of dietary high doses of vitamin E on Labeo rohita fingerlings. Five experimental diets were formulated to contain one control (VE0), two adequate levels of vitamin E supplementation, i.e. 1…
29 Apr 2019DOI 10.17582/journal.pjz/2019.51.4.1211.1219
Research ArticleMorphology of Immature Stages of Schistocerca gregaria with Special References to its Size Variations
Ahmed Ali Samejo, Riffat Sultana
Adults as well as immature of Schistocerca gregaria are voracious vegetation consumer and destructive pest to the useful plants. Morphology and morphometry of immatures of desert locust in relation to gaining of body length and weight at each consecutive stage…
29 Apr 2019DOI 10.17582/journal.pjz/2019.51.4.1221.1226- Short Communication
Evidence of Brucella abortus in Non-Preferred Caprine and Ovine Hosts by Real-time PCR Assay
Muhammad Zain Saleem, Raheela Akhtar, Asim Aslam, Muhammad Imran Rashid, Zafar Iqbal Chaudhry, Muhammad Adeel Manzoor et al.
The present study was conducted in Sheikhupura and Kasur districts for molecular based confirmation of B. abortus in both sheep and goats. Brucellosis in sheep and goats is caused by B. ovis and B. melitensis, respectively but B. abortus (causative agent of bo…
28 Apr 2019DOI 10.17582/journal.pjz/2019.51.3.sc3 - Short Communication
Nutritional Evaluation of Two Sorghum Varieties in Broiler Fortified with Phytase
Asad Sultan, Rabia Ali, Rifat Ullah Khan, Sarzamin Khan, Naila Chand, Ambrina Tariq
Poultry industry is largely based on cereals for energy component in poultry ration. However, certain anti-nutritional factors hamper the bioavailability of nutrients and need to be addressed to avoid compromised birds’ performance. The present stu…
27 Apr 2019DOI 10.17582/journal.pjz/2019.51.3.sc2 
Research ArticleGenetic Diversity among some Horse Breeds in Pakistan
Faiz-ul Hassan, Muhammad Sajjad Khan, Muhammad Saif-ur-Rehman, Syed Muhammad Raihan Dilshad, Muhammad Amjad Ali
Genetic diversity among 12 horse breeds present in Pakistan was determined by using RAPD markers. The blood samples of purebred animals of eight exotic breeds (Thoroughbred, Arab, Anglo-Arab, Suffolk, Percheron, Clevland, Noriker and Hanovarian) present…
26 Apr 2019DOI 10.17582/journal.pjz/2019.51.4.1203.1209
Short CommunicationDiversity of Retrotransposons (Ty3-gypsy, LINEs and Ty1-copia) in Sordaria fimicola
Tazeen Jamil, Saba Ijaz, Rabia Arif, Faiza Akram, Muhammad Saleem
Retrotransposons have become an inevitable source of genetic diversity due to their abundance in eukaryotic genomes and their insertion in coding regions leads to altered gene expression. We examined the diversity of retroelements in four consecutive gen…
24 Apr 2019DOI 10.17582/journal.pjz/2019.51.3.sc6- Research Article
Effect of Dietary Vitamin E, Selenium and Their Combination on Concentration of Selenium, MDA, and Antioxidant Enzyme Activities in Some Tissues of Laying Hens
Åžaban Çelebi
This experiment was conducted to investigate the effects of dietary vitamin-E (α-tocopherol acetate), selenium (selenomethionine) and their combination on Se concentration in liver, heart, kidney and breast muscle. In addition, malondialdehyde (MDA…
23 Apr 2019DOI 10.17582/journal.pjz/2019.51.3.1155.1161 
Research ArticleMolecular Identification and Characterization of Lactic Acid producing Bacterial Strains Isolated from Raw and Traditionally Processed Foods of Punjab, Pakistan
Sohaib Afzaal, Usman Hameed, Nasir Ahmad, Naeem Rashid, Muhammad Saleem Haider
Seventeen (17) wild strains of lactic acid producing bacteria (LAB) were screened, out of one hundred and forty-four (144) bacterial strains isolated from different local food groups, on the basis of stress tolerance assays. Identification of the screened stra…
23 Apr 2019DOI 10.17582/journal.pjz/2019.51.3.1145.1153
Research ArticleImmunohistochemical Expression of Transforming Growth Factor-Alpha between Rat Submandibular and Sublingual Salivary Glands during Post-Natal Development
Shaher Bano, Nadia Naseem, Sarah Ghafoor
Transforming growth factor-alpha (TGF-α) is a mitogen polypeptide that regulates the pre and post-natal development, structure and function of salivary glands. The localization of TGF-α in post-natal submandibular gland (SMG) of rats and huma…
18 Apr 2019DOI 10.17582/journal.pjz/2019.51.3.1099.1103