Articles
3,478 open-access articles · page 162 of 174
Research ArticleConstruction and Characteristic Analysis of Omp10 Deletion Mutant of Brucella abortus
Tiansen Li, Meiling Huang, Zhen Wang, Fei Guo, Hui Zhang, Chuangfu Chen
Brucella is pathogenic bacteria that cause animal and human brucellosis. Currently, the mechanism behind the pathogenesis of Brucella remains unclear. For this reason omp10 mutant was constructed to examine the impacts of the outer membrane protein 10 (Omp10)…
14 Sept 2017
Research ArticleRoot Rot Disease Complex of Cotton: A Menace to Crop in Southern Punjab and its Mitigation through Antagonistic Fungi
Muhammad Arslan Khan, Sajid Aleem Khan, Imran-ul-haq, Rashad Waseem Khan
Root rot is very drastic disease prevalent in major cotton producing areas of Pakistan. Complex nature of disease due to root rot fungi and root-knot nematode cause huge losses to cotton. Experiments were conducted to assess the role of fungi and root knot nem…
14 Sept 2017
Research ArticleA Recurrent Mutation in CTSK Gene is Responsible for Autosomal Recessive Pycnodysostosis in Consanguineous Pakistani Families
Mehran Kausar, Naveed Ashraf, Farzana Hayat, Asraf Hussain Hashmi, Saima Siddiqi, Mariam Anees
Pycnodysostosis is a rare genetic bone disorder (OMIM 265800) with an autosomal recessive mode of inheritance. More than 50 mutations have been reported in CTSK (Cathepsin k) responsible for this disease. Mutations in CTSK result in impaired bone resorption co…
11 Sept 2017
Research ArticleField Evaluation of Selective Systemic Formulations against Sucking Insect Pest Complex and their Natural Enemies on a Transgenic Bt Cotton
Talha Nazir, Muhammad Dildar Gogi, Muhammad Zeeshan Majeed, Waheed ul Hassan, Abdul Hanan, Muhammad Jalal Arif
Sucking insect pest complex, comprising of aphids Aphis gossypii Glover, jassids Amrasca devastans Distant, whiteflies Bemisia tabaci Gennadius, thrips Thrips tabaci Lindeman, red cotton bugs Dysdercus koenigii Walk and dusky cotton bugs Oxycarenus hyalinipenn…
11 Sept 2017
Research ArticleInfluence of Feeding Rate on the Growth, Feed Efficiency and Carcass Composition of the Giant Gourami (Osphronemus goramy)
Netti Aryani, Azrita, Ainul Mardiah, Hafrijal Syandri
Aquaculture feeding rate is an important factor affecting the growth of giant gourami. The aims of the study were to investigate the effect of different feeding rates on the growth, and carcass composition of giant gourami (initial weight 14.17±0.15 g a…
8 Sept 2017
Research ArticleEfficacy of Nuclear Polyhedrosis Virus and Flubendiamide Alone and in Combination against Spodoptera litura F.
Sumaira Maqsood, Muhammad Afzal, Muhammad Anjum Aqueel, Abu Bakar Muhammad Raza, Waqas Wakil, Muhammad Hussnain Babar
Nuclear polyhedrosis virus (NPV) is an important potential pathogen which can control a wide range of bollworm larvae in both agricultural and horticultural crpos. Flubendiamide is a new chemistry insecticide which also controls bollworm effectively and relati…
8 Sept 2017
Research ArticlePhylogenetic Relationship of Locally Isolated Paramecium Species Inferred from Histone H4 Genes
Fareeda Tasneem, Farah Rauf Shakoori, Abdul Rauf Shakoori
The histone H4 gene is supposed to be highly conserved among all organisms with the exception of variations found in the ciliate species. Thus a fragment of histone H4 gene (160bp) was sequenced in ten locally isolated strains of Paramecium species including a…
8 Sept 2017
Research ArticleSpatial Distribution of Soil Borne Brucella Species Specific DNA in Punjab, Pakistan
Rais Ahmed, Khushi Muhammad, Masood Rabbani, Muhammad Sarwar Khan
Brucella species are cause of abortions and other reproductive disorders in animals and human beings. In this project, prevalence and distribution of soil borne Brucella species in nine districts of Punjab including Lahore, Faisalabad, Sheikhupura, Sargodha, D…
7 Sept 2017
Research ArticleBiodiversity and Species Distribution of Coccinellids (Coccinellidae: Coleoptera) in District Sargodha (Punjab), Pakistan
Kanwer Shahzad Ahmed, Muhammad Zeeshan Majeed, Muhammad Ather Rafi, Fatima Sellami, Muhammad Afzal
Coccinellid beetles (Coccinellidae; Coleoptera) are voracious predators of many insect pests of economic importance. Among 6,000 species of coccinellids described worldwide, only 75 species have been reported from Pakistan so far. Keeping in view the limited w…
7 Sept 2017
Research ArticleDetection of Insecticide Residues in Honey of Apis dorsata F. from Southern Punjab, Pakistan
Muhammad Aslam Farooqi, Mansoor-ul-Hasan, Sohail Akhtar, Muhammad Arshad, Muhammad Naveed Aslam, Muhammad Rafay
A simple and fast analytical method using High Performance Liquid Chromatography with ultraviolet detection (HPLC-UV) was used to determine residues of commonly used agricultural insecticides (imidacloprid, thiametoxam, profenofos, endosulfan, spinosad and del…
7 Sept 2017- Short Communication
Evaluating Varying Calf Milk Replacers for Optimum Growth Performance in Salt Range Lambs
Muhammad Fiaz, Khadija Munawar, Muhammad Mushtaq, Mukhtar Ahmad, Mushtaq Ahmad Chaudhry, Muhammad Aslam et al.
The objective of study was to evaluate through feeding calf milk replacer to lambs for optimum growth performance and survivability. For this purpose, Salt Range neonatal lambs (n = 24, age = 01 week) were selected and divided equally into three groups having…
6 Sept 2017 - Short Communication
Optimization of Acridine Orange Staining for Buffalo Sperm, Cryopreserved in Egg Yolk based Extender to Detect DNA Fragmentation
Mushtaq Ahmad, Mehboob Ahmed, Nasim Ahmad
The integral genome of sperm is crucial for successful fertilization and embryonic development. Different techniques have been used to determine the integrity of DNA in which acridine orange (AO) assay is easy and less costly. Though used in many species but t…
6 Sept 2017 
Research ArticleAssessment of Feeding Value of Processed Rice Husk for Lohi Sheep in Growing Phase
Rahat Naseer, Abu Saeed Hashmi, Zulfiqar-ul-Hassan, H. Rehman, Saima Naveed, F. Masood et al.
The present project was envisaged by using rice husk as a substrate for processing with the objective to produce a nutritionally enriched bulk feed ingredient and to explore its potential as wheat bran replacer in total mixed rations for the ruminants. For thi…
5 Sept 2017
Research ArticleEstimation of Cold Carcass Weight and Body Weight from Several Body Measurements in Sheep through Various Data Mining Algorithms
Ali Karabacak, Senol Celik, Adile Tatliyer, Ismail Keskin, Yakup Erdal Erturk, Ecevit Eyduran et al.
The goal of the present study was to compare the predictive performance of three data mining algorithms viz., CHAID, Exhaustive CHAID, and CART implemented in the estimation of cold carcass weight (CCW) and body weight (BW) from several body measurements (with…
5 Sept 2017
Research ArticleGrowth Performance and Nutrient Digestibility of Cirrhinus mrigala Fingerlings Fed Phytase Supplemented Sunflower Meal Based Diet
Syed Makhdoom Hussain, Tasneem Hameed, Muhammad Afzal, Arshad Javid, Nosheen Aslam, Syed Zakir Hussain Shah et al.
A feeding trial was conducted to study the effects of graded levels (0, 500, 1000, 1500 and 2000 FTU kg-1) of microbial phytase on growth performance and nutrient digestibilityof Cirrhinus mrigala fingerlings fed sunflower meal based diet. The chromic oxide wa…
5 Sept 2017
Research ArticleMolecular Phylogeny and Diversity Analysis of Hog Deer (Axis porcinus) in Pakistan
Ghulam Abbas, Asif Nadeem, Masroor Ellahi Babar, Tanveer Hussain, Muhammad Sajid Tahir, Wasim Shehzad et al.
Many species of mammals have declined within the past two centuries due to human caused disturbances and the unsustainable use of natural resources. Molecular methods have an important role in phylogeny and diversity analysis. In Pakistan hog deer (Axis porcin…
30 Aug 2017
Research ArticleMolecular Cloning and Expression of Taste Receptor Gene T2R1 of Obscure Puffer, Takifugu fasciatus
Yijin He, Song Ma, Bo Liu, Ting Xue, Qunlan Zhou, Wu Jin et al.
Obscure puffer, Takifugu fasciatus is a unique species of China which is distributed in the estuary of the Yangtze River, and belongs to the same genus with Red fin puffer T. rubripes which has been used as one of human genome model animal since mid-1990s. In…
30 Aug 2017
Research ArticleNew Hipparionine (Perissodactyla) Fossils from Potwar Plateau of Pakistan
Muhammad Hanif, Naureen Rana, Muhammad Akbar Khan, Muhammad Javed, Muhammad Sajjad Khan
New dental material of hipparionines from the Middle Siwalik deposits of district Attock, Potwar Plateau, Punjab, Pakistan has been described. The fossiliferous sites, Dhok Mila of Nagri Formation (early Late Miocene) and Kaulial Kas of Dhok Pathan Formation (…
30 Aug 2017- Obituary Note
Obituary Note: Prof. Dr. Muzaffer Ahmad (1920-2016)
Prof. Dr. A.R. Shakoori
Obituary Note: Prof. Dr. Muzaffer Ahmad (1920-2016)
28 Aug 2017 
Research ArticleAntimicrobial and Anti-Prostate Cancer Activity of Turmeric (Curcuma longa L.) and Black Pepper (Piper nigrum L.) used in Typical Pakistani Cuisine
Saba Irshad, Ayesha Ashfaq, Ammara Muazzam, Abida Yasmeen
Plant extracts have been used as an active antimicrobial compound since long. The present study was intended to validate the antimicrobial, antidiarrheal and anticancer activity of turmeric (Curcuma longa L.) and black pepper (Piper nigrum L.), used in Pakista…
25 Aug 2017