Background: FMD virus poses a serious threat to the inter-national animal trade. The preventive vaccinations followed by sero-survey have been implemented for the control of FMD. Objectives: 3ABC ELISA is highly effective for early identification of infected and vaccinated animals. Our study was designed for assessment of vaccination by local and imported FMD vaccines in cattle sera in Kalubeya Governorate. Methods: 220 serum samples were tested for FMD NSP by 3ABC Trapping ELISA.20 serum samples were used as control (no vaccination),100 serum samples collected from animals received local vaccine and 100 serum samples from animals received imported vaccine. Results: 34/220(15.5%) were +ve and 186/220(84.5%)were –ve for FMD NSP test preinoculation with vaccines.Serotyping of FMD antibodies were adopted on examined sera for imported and local vaccines using solid phase competitive ELISA 2,4,8,and 10 weeks post vaccination(wpv). Results were 45(24.2%), 65(34.9%)2 WPVand 55(29.5%) ,68(36.5%) 4WPV- 58(31.2%),71 (38.2%) 8WPV and 58(31.2%), 69(37.1%) 10 WPV for serotype A.For serotype (O):55(29.5%) ,58(31.2%)2WPVand 60(32.3%), 62(33.3%) 4WPV and 62(33.3.5%) , 62(33.3%) 8 WPV 60 (32.3%),63(33.9%)10 WPV.For serotype (SAT2):30(.16.1%), 22(11.8%) 2WPV and 39(20.9%), 41(22%)4WPV and 37(19.9%),41(22%) 8/ WPV/ and 27(14.5%), 35(18.8%) 10 weeks post vaccination. Conclusion: vaccines are a fundamental strategies aimed at global control and eradication of FMD. Control of FMD requires vaccines that will provide differentiating infected from vaccinated animals (DIVA).