Articles
308 open-access articles · page 1 of 16
Research ArticleIn vitro Culture of Lake Trout (Salvelinus namaycush) Cells and Trials to Isolate Salmonid Herpesvirus-3 (syn. Epizootic Epitheliotropic Disease Virus)
Megan Shavalier, Mohamed Faisal
The lake trout (Salvelinus namaycush) is an ecologically and economically important fish species in the Great Lakes basin of North America. Primary cultures of lake trout cells were produced following manual and enzymatic digestion of tissue and incubation at…
14 Aug 2025DOI 10.17582/journal.jvs/2025/13.01.10
Mini-ReviewA Mini Review on Exploring the Role of Beneficial Viruses
Esraa M. Halawa, Wael S. El-Araby
Most people hold the belief that all viruses are harmful. However, this idea is incorrect, and requires correction. In this review article, we mentioned numerous examples of beneficial viruses to demonstrate their existence. Moreover, we aim to dispel the noti…
14 Aug 2025DOI 10.17582/journal.jvs/2025/13.28.32
ReviewAn Overview of Dual Vaccines in Ruminants
Shahd Khalid, Walid Mousa, Alyaa Elrashedy, Akram Salama, Ahmed Zaghawa, Ahmed Elsify et al.
Vaccination is a cornerstone of animal health, playing a vital role in preventing infectious diseases and safeguarding both livestock and companion animals. By stimulating the immune system to recognize and combat specific pathogens, whether bacterial or viral…
14 Aug 2025DOI 10.17582/journal.jvs/2025/13.11.27
Research ArticleEffect of CMV Infection on Basil Active Ingredients Used to Treat HCV Ayapatients
Khaled Abdel-Fattah El-Dougdoug, Aya Hussin El-Turkey, Wael Sayed El-Araby, Ahmed Ramadan Sofy, Adel Abd El-Based Mousa, Ahmed Abbas
Ocimum basilicum L., commonly sweet basil, belongs to the Lamiaceae family and is characterized by an essential oil primarily composed of monoterpenes, sesquiterpenes, and phenylpropanoids. Viral infections can influence the levels of these compounds. The cucu…
11 Mar 2025DOI 10.17582/journal.jvs/2024/12.2.26.31
ReviewEpidemiological Situation and Spread of Avian Influenza H9N2 in Poultry in North Africa
Hadda Kareche, Ola Elbohy, Janet M. Daly
Avian influenza (AI), which is recognized as one of the most contagious respiratory diseases in poultry worldwide, is caused by avian influenza A virus (LPAIV). Wild birds are considered the primary natural reservoirs of low pathogenicity avian influenza (LPAI…
29 Jan 2025DOI 10.17582/journal.jvs/2024/12.1.10.25
Research ArticleVirus Like Particles: Nanoparticles for Targeted Drug Delivery
Khaled A. El-Dougdoug, Wael S. El-Araby, Rehab, A. Dawoud
Virus-like particles (VLPs) are biological entities at the nanoscale level that are made of viral proteins organized in a morphology that resembles the original virion but lacks the viral genetic material. VLPs are appealing as a system because their proteins…
22 Oct 2024DOI 10.17582/journal.jvs/2023/11.2.25.44
ReviewPlant Virome
Khaled A. El-Dougdoug, Wael S. El-Araby, Rehab A. Dawoud
The viromes of land plants are enormous and diverse, and they are dominated by RNA viruses. However, reverse-transcription and single-stranded (ss) DNA viruses also make significant contributions to the viromes of land plants. In this article, we present a tho…
22 Oct 2024DOI https://dx. doi. org/10. 17582/journal. jvs/2023/12. 1. 26. 45
Research ArticleEvaluation of Humoral Immune Response to Simultaneous Vaccination with Ephemerovirus Febris and Phlebovirus Riftense Vaccines
Aya A. Saber, Mohamed Fawzy, MokhtarM. EL-Tarabili, Eman K. El Sayed
Ephemerovirus febris and Phlebovirus riftense formerly known as Bovine ephemeral fever (BEF) and Rift Valley fever (RVF), respectively, are insect-borne viral diseases that affect cattle, causing significant economic losses. The application of a promising vacc…
5 May 2024DOI 10.17582/journal.jvs/2024/12.1.1.9
ReviewLow Pathogenicity Avian Influenza H9N2 Virus in Africa
Abdel Satar Arafa
Abstract | Avian influenza virus (AIV) of the subtype H9N2 virus has been detected worldwide in wild and domestic birds as well as in few occasions in mammals. The virus was recorded in several African countries, with endemic status in Egypt and frequently rep…
16 Apr 2024DOI 10.17582/journal.jvs/2022/10.2.16.23
Research ArticleAntigenic Characterization and Pathogenicity Study of Recent Field Isolates of Infectious Bursal Disease Viruses
H.A. SULTANl, H.A. HUSSEIN, F.F. EL-KHAYAT
Epidemiological investigation of severe outbreaks of infectious bursal disease (IBD) among vaccinated chicken flocks during the period 2002-2003 revealed that all IBDV outbreaks occurred at 18-33 days of age. 70% (21/30) of these outbreaks characterized…
5 Jan 2024
Research ArticleProduction of FMDV ELISA Kit for Antibodies Detection in Animals Using Native Strain with Production of Reference Hyper-immune Serum in Guinea Pigs
M. E. Shawky, A. M. Daoud
The objective of the present study was to evaluate an enzyme linked immunosorbent assay (ELISA) based kit for detecting foot and mouth disease (FMD) antibodies using a native FMDV strain (O1. Aga 93). Results were compared to those of serum neutralization test…
5 Jan 2024
Research ArticleIsolation and Characterization of a Pseudomonas aeruginosa Bacteriophage from Agriculture Drain Water
Amina A. II, M.K. Amin
Five bacteriophages infecting Pseudomonas aeruginosa strain were isolated from agricultural drain water of Zagazig city. One of them designated as PAI has been characterized. The ability of phage PAI to form plaques with strain PAOI was examined. Two kinds of…
5 Jan 2024
Research ArticlePhylogenetic Characterization of Some Bovine viral diarrhea viruses in Egypt
A. A. El-Kholy, S. Vilcek, A. M. Daoud
Sixty-seven buffy coat specimens, obtained from clinically suspected cattle at farms located in Belbees. El-Sharquia, were screened for BVDV. Only 9/67 of the tested specimens (13%) were BVDV positive by reverse transcription-polymerase chain reaction (RT-PCR)…
5 Jan 2024
Research ArticleCytopathogenicity of Local Field Strains of Bovine Viral Diarrhea Virus Isolated from Apparently Health Buffaloes in Egypt
H. A. Hussein, N.A. Mohamed, F.M. Mohamed, M.A. Shalaby
The growth behavior of BVDV field strains isolated from apparently health buffaloes in cell culture was studied. The isolated viruses were classified into 3 groups based on the onset and severity of cytopathic effect (CPE). The cytopathogenicity of the isolate…
5 Jan 2024
Research ArticleMolecular Characterization of Hyper Variable Region of the Classical and Very Virulent Egyptian Isolates of IBDV
Seham A. El-Zeedy, Dalia A. Abd El-Moaty, H. A. Hussein, M. A. Shalaby
Many recent outbreaks of Infectious bursal disease in commercial chicken flocks worldwide are due to the spread of very virulent IBDV. Two Egyptian isolates Of IBDV isolated from Giza (Giza/2000) and Qalyubia (Kal/2001) governorates were characterized by RT/PC…
5 Jan 2024
Research ArticleDetection of BVDV Associated with Mortalities in Camels
M.R. Abd-El Wahab l, H.A. Hussein, T.M. Asfourl, M.A. Shalaby
BVDV was detected in tissues collected from dead camel cases using antigen capture ELISA and fluorescent antibody assay. Histopathology examination of different organs revealed changes similar to those reported with BVDV infection. The existence of BVDV in ser…
5 Jan 2024
Research ArticleAn Inhibitory Activity of Propolis Extract against Faba Bean Mottle Bromovirus (BBMV)
E.F. Mohamedl, A.A. Owayss
A Bromovirus was isolated from naturally infected faba bean plants in Fayoum Governorate. The virus was identified as Faba bean mottle Virus (BBMV) according to symptomatology on Faba bean plants, host range, diagnostic hosts, virus stability and ELISA detecti…
5 Jan 2024
Research ArticleInduction of Salicylic Acid in Cucumber against ZYMV Potyvirus by some Nutrient Chemicals
M.A. Abo-EInasr l, Kh.A. EL-Dougdougl, M.H. El-Kattan, L.A. Salem
Systemic acquired resistance (SAR) could be induced in cucumber plants using different concentrations of seven nutrient chemicals against Zucchini yellow mosaic Potyvirus (ZYMV). These chemicals were. Potassium sulfate, Magnesium sulfate, Ammonium sulfate, Che…
5 Jan 2024
Research ArticleThe Influence of Gibberellic Acid (GA) and Indole Acetic Acid (IAA) on The Production of Local Lesions on Nicotiana sylvestris Plants Infected with Tomato Mosaic Tobamovirus (ToMV)
E.F. Mohamed, I.M. Sabra
Tomato mosaic disease caused by Tomato mosaic (ToMV) is widespread wherever tomato is grown ToMV affects plants and yields. Virus was isolated and identified on the base of symptomatology on tomato plants, host range, diagnostic hosts, physical ptoperties, mod…
5 Jan 2024
Research ArticleHisto-cytopathological Effects of Cucumber Mosaic Cucumovirus on Squash leaves
M. Osman, Kh. El-Dougdoug, E. T. Abd El-Salam, R.M. Taha, R.M. El-Hamidl
An isolate of Cucumber mosaic cucumovirus (CMV) infected cucumber plants was detected by DAS-ELISA and confinned by inoculation of Chenopodium amaranticolor. CMV-isolate was propagated on squash plants var. Eskandarani. After 15 days. systemic symptoms appeare…
5 Jan 2024
