Articles
308 open-access articles · page 2 of 16
Research ArticleBovine Leukosis in Holstein Cattle in Saudi Arabia as Evidenced by Presence of Specific Antibodies
Musaad A. Al-Dubaib
In the present study, serum samples obtained from Holstein cattle, native cattle, sheep, goats, and camels in Al-Qassim, and surrounding regions were tested for bovine leukemia virus (BLV) antibodies for different animal species. Agar gel immunodiffusion was c…
5 Jan 2024- Research Article
Isolation and Characterization of a Pseudomonas aeruginosa Bacteriophage from Agriculture Drain Water
Amina A. II, M.K. Amin
Five bacteriophages infecting Pseudomonas aeruginosa strain were isolated from agricultural drain water of Zagazig city. One of them designated as PAI has been characterized. The ability of phage PAI to form plaques with strain PAOI was examined. Two kinds of…
5 Jan 2024 - Research Article
Population Fluctuations of Aphids in Relation to Virus Disease Incidence in Egyptian Faba Bean Fields
Mohga M. El-Tahlawey, A. Mandour, AE Aboulata
Infection induced by both Faba bean necrotic virus (FBNYV) and mosaic causing viruses were inspected during Oct. 2003 to Apr. 2004 on Faba bean fields grown in Qalyubia governorate. Population density of seven different aphid species on faba bean was arranged…
5 Jan 2024 - Research Article
Occurrence and Spread of Sugar Beet Rhizomania Disease Caused by Beet Necrotic Yellow Vein Benyvirus in Some Governorates of Egypt
S.Y.M. Mahmoud, M. Hashem
Beet necrotic yellow vein Benyvirus (BNYVV) is the important soilborne virus disease in the production areas of sugar beet. In this study. field survey was carried out in sugar beet growing areas belonging to Kafr-ElSheikh, El-Beheira, El-Dakahlia, El-Gharbia.…
5 Jan 2024 - Research Article
Virus Elimination from Infected Garlic Plants Using Different Techniques
S.A. Sidaros, R.A. Omar, S.A. El-Kewey l, Samaa Abd El-Khalik
Production of garlic virus free plant was attempted by means of chemotherapy, meristem-tip culture, and thermotherapy techniques. Three different meristem sizes (5, 3 and 1 mm long) were excised from three garlic cultivars (Chinese. Italian and Balady). Merist…
5 Jan 2024 - Research Article
Physiological and Histopathological Changes in Potato Virus X and Potato virus Y- Infected Potato Plants
Hanaa H.A.Gomaa, A. F. Moustafal, Kh.A.El-Dougdoug, A.A. Abou-Zeid, S. Y.M. Mahmoud
Potato viruses X and Y were used for study the effect of the two viruses on the metabolism of potato plants. It was found that detectable decrease or dry matter, total nitrogen, tuber Starch, number of starch granules, and total protein. The nucleic acid conte…
5 Jan 2024 - Research Article
Histo-cytopathological Effects of Cucumber Mosaic Cucumovirus on Squash leaves
M. Osman, Kh. El-Dougdoug, E. T. Abd El-Salam, R.M. Taha, R.M. El-Hamidl
An isolate of Cucumber mosaic cucumovirus (CMV) infected cucumber plants was detected by DAS-ELISA and confinned by inoculation of Chenopodium amaranticolor. CMV-isolate was propagated on squash plants var. Eskandarani. After 15 days. systemic symptoms appeare…
5 Jan 2024 - Research Article
An Inhibitory Activity of Propolis Extract against Faba Bean Mottle Bromovirus (BBMV)
E.F. Mohamedl, A.A. Owayss
A Bromovirus was isolated from naturally infected faba bean plants in Fayoum Governorate. The virus was identified as Faba bean mottle Virus (BBMV) according to symptomatology on Faba bean plants, host range, diagnostic hosts, virus stability and ELISA detecti…
5 Jan 2024 - Research Article
Purification, Serology and Prevalence of Broad bean stain Comovirus (BBSV) and Cowpea Aphid Borne Mosaic Potyvirus (CABMV)
S. A. Sidaros, S. A. EL-Kewey, Eman A. H. khattab, M. M. ELsharkawy
BBSV and CABMV were purified from faba bean and cowpea plants, respectively using two different methods. The UV absorption spectrum had maxima and minima at 260 and245 nm, respectively for the two viruses. The absorbance ratios of A and A260/280 of the combine…
5 Jan 2024 - Research Article
Interaction Between Rhizobium Meliloti and Its Specific Viruses in Clover Plants
B. A. Othman, Kh. A. El-Dougdoug, M. H. Abdel Ghaffar, T. F. El-Arabi
Four phages singed as RM1, RM2, RM3, and RM4 specific for Rhizobium meliloti (R meliloti) were isolated from tested rhizosphere soil samples collected from Qalyubia and North Sinai Governorates based on plaque morphology, The extinction spectra of purified pre…
5 Jan 2024 - Research Article
Faba bean necrotic yellows virus (FBNYV) in Egypt: Characterization and Virus-Vector Relationship
A. E. Aboul-Ata, Mohga, M. El-Tahlawey, M. A. Amer, A.M. Mandour
Faba bean necrotic yellows virus (FBNYV) has been isolated and identified as an isolate of FBNYV from Egypt. FBNYV characterization was proved according to the following Criteria: Symptomatology, insect transmission (Aphis craccivora), serological tests (DAS-E…
5 Jan 2024 - Research Article
Attenuated Rift Valley Fever Virus (NMP18) Chemically for Vaccine Development
M.S. Wassel, Elham A. El-Ebiary, Soliman, Y.A.l, El-Sayed, M.M
Serial treatment of RVF virus ZH501 with nitrous acid and 5-flourouracil has been employed to derive an attenuated strain of Rift valley Fever virus (NMP18) for use as alive virus vaccine besides studying its safety, potency, and the duration of immunity in pr…
5 Jan 2024 - Research Article
Phylogenetic Characterization of Some Bovine viral diarrhea viruses in Egypt
A. A. El-Kholy, S. Vilcek, A. M. Daoud
Sixty-seven buffy coat specimens, obtained from clinically suspected cattle at farms located in Belbees. El-Sharquia, were screened for BVDV. Only 9/67 of the tested specimens (13%) were BVDV positive by reverse transcription-polymerase chain reaction (RT-PCR)…
5 Jan 2024 - Research Article
The Influence of Gibberellic Acid (GA) and Indole Acetic Acid (IAA) on The Production of Local Lesions on Nicotiana sylvestris Plants Infected with Tomato Mosaic Tobamovirus (ToMV)
E.F. Mohamed, I.M. Sabra
Tomato mosaic disease caused by Tomato mosaic (ToMV) is widespread wherever tomato is grown ToMV affects plants and yields. Virus was isolated and identified on the base of symptomatology on tomato plants, host range, diagnostic hosts, physical ptoperties, mod…
5 Jan 2024 - Research Article
Cytopathogenicity of Local Field Strains of Bovine Viral Diarrhea Virus Isolated from Apparently Health Buffaloes in Egypt
H. A. Hussein, N.A. Mohamed, F.M. Mohamed, M.A. Shalaby
The growth behavior of BVDV field strains isolated from apparently health buffaloes in cell culture was studied. The isolated viruses were classified into 3 groups based on the onset and severity of cytopathic effect (CPE). The cytopathogenicity of the isolate…
5 Jan 2024 - Research Article
Isolation, Antigenic and Molecular Characterization of Bovine Viral Diarrhea Virus Field Strains from Apparently Health Buffaloes in Egypt
Nahed A. Mohamed l, H. A. Hussein, Fathia M. Mohamed, M. A. Shalaby
In the present study, we report the isolation of BVDV field strains from apparently health buffaloes. One hundred and thirty-two samples including buffy coat and organs were collected from apparently health Screening buffaloes in Cairo, Mansoura and Suez gover…
5 Jan 2024 - Research Article
Comparative Study on Two Thrips Transmitted Viruses Tomato spotted wilt virus (TSWV) and Iris yellow spot virus (IYSV) as a Tospovirus
Manal A. El-Shazly, A. s. 2 Abdel Wahab, Salwa N. Zein
Biological, biochemical and serological comparison between Tomato spotted wilt virus (TSWV) and Iris yellow spot virus (IYSV) a Tospovirus were studied These two viruses were isolated from Vinca rosae (L). G. Don and onion (Allium cepa L.), respectively. The o…
5 Jan 2024 - Research Article
Detection of BVDV Associated with Mortalities in Camels
M.R. Abd-El Wahab l, H.A. Hussein, T.M. Asfourl, M.A. Shalaby
BVDV was detected in tissues collected from dead camel cases using antigen capture ELISA and fluorescent antibody assay. Histopathology examination of different organs revealed changes similar to those reported with BVDV infection. The existence of BVDV in ser…
5 Jan 2024 - Research Article
Induction of Salicylic Acid in Cucumber against ZYMV Potyvirus by some Nutrient Chemicals
M.A. Abo-EInasr l, Kh.A. EL-Dougdougl, M.H. El-Kattan, L.A. Salem
Systemic acquired resistance (SAR) could be induced in cucumber plants using different concentrations of seven nutrient chemicals against Zucchini yellow mosaic Potyvirus (ZYMV). These chemicals were. Potassium sulfate, Magnesium sulfate, Ammonium sulfate, Che…
5 Jan 2024 - Research Article
Isolation and Characterization of a Pseudomonas Putida Bacteriophages
Mayada A. Abd Elgalell, B.A Othman, Th. Radwan, Amal S.M. Abo-Sinna
Two phage isolates for Pseudomonas putida were isolated from soil cultivated with potato by the enrichment culture technique and signed as PPI and PP2. The phages were propagated and purified by polyethylene glycol, 6000-sodium dextran sulfate 500 two phase sy…
5 Jan 2024