Articles
308 open-access articles · page 3 of 16
Research ArticleHisto-cytopathological Effects of Cucumber Mosaic Cucumovirus on Squash leaves
M. Osman, Kh. El-Dougdoug, E. T. Abd El-Salam, R.M. Taha, R.M. El-Hamidl
An isolate of Cucumber mosaic cucumovirus (CMV) infected cucumber plants was detected by DAS-ELISA and confinned by inoculation of Chenopodium amaranticolor. CMV-isolate was propagated on squash plants var. Eskandarani. After 15 days. systemic symptoms appeare…
5 Jan 2024- Research Article
Interaction Between Rhizobium Meliloti and Its Specific Viruses in Clover Plants
B. A. Othman, Kh. A. El-Dougdoug, M. H. Abdel Ghaffar, T. F. El-Arabi
Four phages singed as RM1, RM2, RM3, and RM4 specific for Rhizobium meliloti (R meliloti) were isolated from tested rhizosphere soil samples collected from Qalyubia and North Sinai Governorates based on plaque morphology, The extinction spectra of purified pre…
5 Jan 2024 - Research Article
Phylogenetic Characterization of Some Bovine viral diarrhea viruses in Egypt
A. A. El-Kholy, S. Vilcek, A. M. Daoud
Sixty-seven buffy coat specimens, obtained from clinically suspected cattle at farms located in Belbees. El-Sharquia, were screened for BVDV. Only 9/67 of the tested specimens (13%) were BVDV positive by reverse transcription-polymerase chain reaction (RT-PCR)…
5 Jan 2024 - Research Article
Re-Emergence of Very Virulent IBDV in Egypt
Ashraf M. Metwally, Ausama A. Yousif*k, Iman B. Walaa A. Attia M. Samy, Ismail M. Reda
Infectious bursal disease (IBD) serotype 1 viruses continue to cause major economic losses in the Egyptian poultry industry despite the implementation of intensive vaccination programs. A recent increase in IBD related mortality in vaccinated farms prompted th…
4 Jan 2024 - Research Article
Immune Response to a Live Attenuated Chicken Anemia Virus (CAV) Vaccine. The Absence of Virus Shedding
Hanan M.M. Elzahed, Abd EL-Moneim A.A, Afaf A. Khedr, Elham A. Elabiare, Waleed S. Abd El-Latif, Reda M.I
An assessment of the performance of a live attenuated (CAV) vaccine following administration to broiler breeder hens in eight houses at Il weeks of age. Antibodies to (CAV) in adult hens and maternally derived antibodies to (CAV) in progeny chicks were a…
4 Jan 2024 - Research Article
In Vitro and In Vivo Evaluation of Inactivated Rift Valley Fever Virus Vaccine
Eman, M. M. Soliman, Taha, M. M, El-Sanousi A, Shalaby M. S. Wasscl et al.
In this study, 5 batches of inactivated rift valley fever (RVF) virus vaccine (ZH501) were evaluated according to Office Internationale Epizooties (OIE) RVF evaluation protocol. The tested batches proved to be sterile and safe when inoculated subcutaneously (S…
4 Jan 2024 - Research Article
HepG2 Cell Culture Infection by Hepatitis C Virus Genotype 4: Viral Proteins and RNA
El-Tabakh, SAA l, Abdel Wahab, KSE, Badr, Helal
Genotype 4 hepatitis C virus (HCV) in viremic serum was used for experimental infection of a human hepatoma cell line (HepG2) at a multiplicity of infection (MOL) of one genome copy per 103 cells. Stock HepG2 cell cultures were grown in Dulbecco modified minim…
4 Jan 2024 - Research Article
Preparation and Evaluation of Inactivated Oil Emulsion Avian Influenza H5N2 Virus Vaccine for the First Time in Egypt
Abd Elwanis, N.D, Abou El Khair, M.A, Afaf H. Amin, Azab et al.
A Whole virus inactivated oil emulsion vaccine has been prepared from the low pathogenic avian influenza (LPAI) virus A/Turky/CA/209092/02, H5N2 for the first time in Egypt. Virus strain from which the vaccine seed virus has been prepared was derived from the…
4 Jan 2024 - Research Article
The discovery of phytoplasmas: A historical reminiscence of success and failure
Karl Maramorosch
Described as virus diseases are now known to be caused by phytoplasmas and spiroplasmas. Earlier attempts to concentrate, purify and visualize by electron microscopy the presumptive viruses have failed. Causative agents of yellows-type plant diseases were clas…
4 Jan 2024 - Research Article
Differentiation among Three Egyptian Isolates of Citrus Psorosis Virus
El-Dougdoug, Kh.A. t, S.A. Ghaza, A.A. Mousa, H. Fahmyj, A.R. sofy
Three Egyptian isolates of Citrus psorosis virus (CPsV-EG), namely ARC, TB and TN were obtained from citrus cvs. Grapefruit, Balady and Navel, respectively. These isolates Were differed in some of their external symptoms. The CPsV-EG isolates were detect…
4 Jan 2024 - Research Article
Evaluation of Hematological and Biochemical Changes observed in HCV Patients with Different Level of Viremia
Fadia M. Attia, Hanaa H. A. Gomaa
HCV viremic status is associated with many hematological and biochemical, immunological abnormalities in patients with chronic hepatitis C. Aim of the study: to describe different patterns of changes accompanying viremic status in HCV chronic liver diseases. 5…
4 Jan 2024 - Research Article
Cloning and Sequencing of a cDNA Encoding the Coat Protein of an Egyptian Isolate of Pepper Mild Mottle Virus
S.A. Sidaros, S.A. El-Kewey, Hala A. Amin, Eman A.H. Khatab, A.A. Emeran l, Samaa Abd El-Khalilk et al.
A Tobamovirus isolated from pepper plants grown in Egypt has been characterized. Pepper mild mottle Tobamovirus (PMMoV) was isolated from naturally infected pepper plants grown in Kafr El-sheikh Governorate. RT-PCR using a specific non:åegenerate primer…
4 Jan 2024 - Research Article
Vaccination Trial in Cattle using Mp12 Live Attenuated Rift Valley Fever Virus
Manva F.M. Abdel Monem, Gihan K. Mohammed, Sami A.M, Saber M.S
The present study was aimed to evaluate the live mutagenized RVF-MP12 vaccine in 4 months old calves. The used dose was 5 log10 TCID5Janimal. The safety and potency of the vaccine was followed up as well as the duration of the immunity by ELISA and SNT through…
4 Jan 2024 - Research Article
Medicinal Plants Used in the traditional Treatment of Several Viral Diseases in Bangladesh
Mazharul A. M, Md. S. Sarwar, M. Nurullah, Keshab K. Adhikary I, M. Rahmatullah
Viral fevers, measles, chicken pox, and rabies are fairly common occurrences throughout Bangladesh. Since the population of the country is predominantly rural without proper access to modern medical facilities, they rely on traditional medical practitiofiers (…
4 Jan 2024 - Research Article
Comparative Molecular Analysis of Isolated Strains of Highly Pathogenic Avian Influenza Virus H5N1 during 2006 and 2007
Madbouly, H.M, Orkhan, M.H, Nagwa El-khoty
Highly pathogenic avian influenza (Al) H5N1 strains have been isolated during February 2006 from infected chicken located at El-Qanater El-Khiria center, Qaluobia, Eypt. These isolates were molecularly characterized and compared with those H5Nl strains is…
4 Jan 2024 - Research Article
Possible Causes of Re-Emerging outbreaks of H5N1 Avian Influenza virus in Vaccinated Chickens in Sharkia Governorate In Egypt
Hussein, sultan, HA. Al Deeb AH, AA. El-Sanousi
Re-emerging of H5Nl severe outbreaks in vaccinaied chickens housed in poultry farms in Sharkia governorate in Egypt was observed starting from October 2007 and continued for 3 months until control measures have been taken in such farms. besides multiple vaccin…
4 Jan 2024 - Research Article
Effect of Silnultaneous Vaccination with the Bivalent Foot and Mouth Vaccine and Bovine Ephemeral Fever Vaccine on the Immune Response OF Cattle
Attyat, M. Kotb, Abeer, E. Mansour, Naglaa, I. Aly et al.
The present study was aimed to evaluate the vaccination of cattle against bovine ephemeral fever (BEF) and bivalent foot and mouth disease (FMD) strain O and strain A. so fifteen calves, six month old, were vaccinated singly and simultaneously and two calves w…
4 Jan 2024 - Research Article
Synthesis of the Bovine Rotavirus Major Neutralization Antigen (VP7) Using A Baculovirus Expression System
El-Sabagh, I.M, Hussein, H.A, Amer, H.M et al.
The present study reports the successful production of the major neutralization antigen (VP7) of bovine romvirus Nebraska calf diarrhea virus (NCDV) strain in insect cells. The full-length DNA copies of RNA segment 9 (coding for VP7 protein) of NCDV was insert…
4 Jan 2024 - Research Article
Isolation of Bovine Viral Diarrhea Virus from Calves using Rabbits and Comparative Detection of both Viral Antigen and Antibody for BVDV Genotypes I & II in Experimentally Inoculated Rabbits
Ahmed Abd El Samie Hassan Ali
Bovine viral diarrhea virus (BVDV) is a world wide pathogen infects a wide variety of ruminants. A random 42 field pooled calf samples were collected and inoculated simultaneously intranasal, oral and intraperitoneal into 6 New Zeeland rabbits. The rabbits wer…
4 Jan 2024 - Research Article
Isolation and Antigenic and Molecular Characterization of G10 of Group A Rotavirus in Camel
Eman, Abo Hatab, Hussein, El-Sabagh IM, Saber
We are here reporting the successful Isolation and characterization of GIO serotype of group A rotaviruses from fecal samples collected from camel farms suffering from diarrhea in Alexandria and Esmalia governorates. After preparation of fecal samples and inoc…
4 Jan 2024