Articles

Preparation and Evaluation of Inactivated Oil Emulsion Avian Influenza H5N2 Virus Vaccine for the First Time in Egypt

Abd Elwanis, N.D, Abou El Khair, M.A, Afaf H. Amin, Azab et al.

A Whole virus inactivated oil emulsion vaccine has been prepared from the low pathogenic avian influenza (LPAI) virus A/Turky/CA/209092/02, H5N2 for the first time in Egypt. Virus strain from which the vaccine seed virus has been prepared was derived from the…

Isolation of Bovine Viral Diarrhea Virus from Calves using Rabbits and Comparative Detection of both Viral Antigen and Antibody for BVDV Genotypes I & II in Experimentally Inoculated Rabbits

Ahmed Abd El Samie Hassan Ali

Bovine viral diarrhea virus (BVDV) is a world wide pathogen infects a wide variety of ruminants. A random 42 field pooled calf samples were collected and inoculated simultaneously intranasal, oral and intraperitoneal into 6 New Zeeland rabbits. The rabbits wer…

The discovery of phytoplasmas: A historical reminiscence of success and failure

Karl Maramorosch

Described as virus diseases are now known to be caused by phytoplasmas and spiroplasmas. Earlier attempts to concentrate, purify and visualize by electron microscopy the presumptive viruses have failed. Causative agents of yellows-type plant diseases were clas…

Study on the Infection of Zoo Birds by Highly Pathogenic H5N1 Avian Influenza Virus

Arafa, El-Kanawaty, Hassan, M.K, Anwar, H.K et al.

From February to June 2006 an epidemic of highly pathogenic avian influenza (HPAI) virus Of subtype H5N1 affected all commercial poultry sectors as well as rural and backyard level in Egypt. This outbreak also was extended to include the zoo birds In Giza zoo.…

WHO Global Influenza Program Surveillance and Network, 2005; (www.oie.int). Outbreak of highly pathogenic avian influenza (Al) H5N1 in poultry in Egypt 2006

Madbouly, H.M, Orkhan, M.H, Nagwa El-khoty

Avian influenza outbreak was firstly appeared in poultry reared in backyards or roofs of homes in Qaluobia & Giza governorates as many notifications were received by the General Organization of Veterinary Services (GOVSs) in February 2006. The disease was…

Evaluation of Some Therapies to Eliminate Potato Y Potyvirus from Potato Plants

Sabry Y. M. Mahmoud, Maher H. Hosseny, Mamdouh H. Abdel-Ghaffar

Viral infection of potato (Solanum tuberosum L.) plants is very important due to their effect on potato yield and degeneration of seed tubers. This study aimed to eliminate potato virus Y (PVY) by tissue culture technique using different therapies, such as the…

Study on Infectious Bursal Disease (GUMBORO) in Quails

Abou zead, A.A, Ali, A.A.H, Abd El Hkeem M.M

Eighty migratory quails from EL-Areesh coast were trapped. Out of them, 18 quails (22.5%) were diseased and suffering mainly from digestive disorders. Mortality rate, as well as the characteristic clinical signs, , post mortem lesions and histopathological cha…

Evaluation of Simultaneous Vaccination against Brucella and Rift Valley Fever Diseases in Sheep

Afaf, A. Khedr, Hyam Farouk, Taradi, A.Said

Brucellosis and Rift valley fever are major diseases affecting sheep flocks where they cause substantial economic losses in sheep and goats flocks. This study was planned to experimental evaluation of simultaneous vaccination against Brucella and attenuated RV…

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Some Factors Affecting Immune Response of Sheep Vaccinated with Bivalent Foot and Mouth Disease Vaccine

M.A. Farag, Abeer, E. Mansour, s.M

The effect of bovine viral diarrhea virus (BVDV) infection and Aflatoxine BI (AFBI) on the immune response of sheep vaccinated with bivalent Foot and mouth disease (FMD) vaccine were studied. Significant decrease in humoral immune response against the vaccine…

The Immune Response of Goats to Concomitant Vaccination with Live Peste Des Petits Ruminants (PPR) and Inactivated Bivalent Foot and Mouth Disease (FMD) Vaccines

Laila, A. Sedeek, Fatma, S. Mohamed, Manal Abo El-Yazyed

The present work was designed to investigate the immune response of goats to the concomitant immunization with PPR and bivalent FMD (type A and O) vaccines. The study included four groups of local breed goats, where the first received modified live PPR virus v…

Comparative Molecular Analysis of Isolated Strains of Highly Pathogenic Avian Influenza Virus H5N1 during 2006 and 2007

Madbouly, H.M, Orkhan, M.H, Nagwa El-khoty

Highly pathogenic avian influenza (Al) H5N1 strains have been isolated during February 2006 from infected chicken located at El-Qanater El-Khiria center, Qaluobia, Eypt. These isolates were molecularly characterized and compared with those H5Nl strains is…

Preparation and Evaluation of Lumpy Skin Disease Hvperimmune Serum Coniugated with Fluorescein

Abdel Razek, B.Omar, Magda M.Sayed

Hyperimmune serum against lumpy skin disease virus(LSDV) was prepared by inoculation of the virus in successive doses into susceptible two calves with complete and incomplete Freund’s adjuvant. Identity tests were applied by agar gel precipitation test (…

Elimination of Grapevine Fan Leaf Virus (GFLV) and Rapevine Leaf Roll-Associated Virus-1 (GLRaV-1) from Infected Grapevine Plants using Meristem Tip Culture

Sahar, A. Youssef t, M.M.A. Al-Dhaher, A.A. Shalaby

Grapevine (Vitis vinifera L. cv. Thompson seedless) was found to be infected with viral diseases showing thicker leaves than normal, brittle, with margins rolled downwards and yellowish, which identified as Grapevine leaf roll-associated virus-I (GLRaV-l). Oth…

Studies on the Preparation of an Improved Foot and Mouth Disease oil vaccine

Sonia, A.M, El. Sanousi, A.A, Saber, M.M et al.

This study has been pursued as a trial for preparation and evaluation of an improved Foot and Mouth disease oil vaccine by using four different adjuvants which are: Montanide ISA 206, Quil A saponine, Ginseng extract and Montanide IMS 3015. These vaccines were…

Preparation and Evaluation of Inactivated Oil Emulsion Vaccine Against Swollen Head Syndrome in Chickens

Hala M. El-Makaky*•, Sherif, N.A, Fekria El-Bordiny, Taha, M.M

In the present study, inactivated single swollen head syndrome oil emulsion vaccine (SHSV) was prepared and evaluated. SHSV was successfully propagated on the yolk sac of 5-7 days of SPF embryonated chicken eggs. The virus was harvested 48-72 hours post inocul…

Baculovirus Expression of the Bovine Coronavirus Nucleocapsid Protein in Spodopetra Frugiperda Insect Cells

Amer, H.M, Hussein, H. A, El-Sabagh, I. M et al.

In the current study, cloning and expression of the bovine coronavirus (BCV) nucleocapsid protein was carried out in a baculovirus expression system. The specific RT-PCR product of N gene was cut and extracted from gel using DNA gel extraction kit (Millipore).…

Comparative Molecular Studies on Different Isolates of IBR Virus

Hassanein, S.A, Ibrahim, A.K, Mervat, M.M et al.

Reference strains and field isolates of herpesviruses recovered from cattle, sheep and goat in Egypt were compared by virus neutralization, (VN), Restriction Fragment Length Polymorphism (RFLP) and Random Amplified Polymorphic DNA (RAPD) analysis. Antigenic co…

Single-Step Multiplex Reverse Transcription-Polymerase Chain Reaction (m-RT-PCR) for Simultaneous Detection of Five RNA Viruses Affecting Stone Fruit Trees

Sahar A. Youssef I, A. A. Shalaby

A single-step multiplex reverse transcription-polymerase chain reaction (m-RTPCR) was developed for the simultaneous detection and discrimination among five RNA viruses namely ,4pple cholorotic leaf spot virus (ACLSV), Prune dwarf virus (PDV), Prunus necrotic…

Biological and Molecular Characterization of Potato Infecting Alfalfa Mosaic Virus in Egypt

El-Helaly, Sahar H, Ahmed, Amal A, Awad, M.A et al.

Alfalfa mosaic virus (AMV) was isolated from naturally infected potato (Solanum tuberosum, L. cv. Spunta) plants, showing bright yellow blotching or mottling «Calico» and aucuba symptoms suspected to be due to virus infection. Leaf samples were col…