Wood samples of Juniper, Spruce, Fir, Deodar, Yew and four pine species were collected from different coniferous forest of Pakistan. The objective was to study their microscopic structure for the preparation of an identification key based on minute anatomy. Results showed clear variations in the occurrence, number, size and type of different anatomical structures, such as resin canals, wood rays pits ray crossing, ray tracheids, procumbent cells, vascular tracheids, pits on vascular tracheids and pit pores. On the basis of these anatomical variations a key is prepared for the identification of important conifers of Pakistan.