FMD is a highly contagious viral disease of all cloven-footed animals and widely distributed all over the world. In this study 465 serum samples were collected from 3 Nile delta governorates (Behaira, Mounofya and Kafer El-Sheikh) during 2009. The samples were used for detection of FMD antibodies to 3ABC non-structural proteins using commercial ELISA kit (Priocheck). The overall percentage of positive was 38.9 %. The higher percentage of positive detected in Behaira (48%), then Mounofya (45.3%) while Kafer El-sheikh was the lowest (23.7%). The positive results of detection of antibodies against non structured proteins of FMDV indicate that these samples come from natural infected animals.