Issue · 2017
Vol.3, Iss. 1
14 articles · All issues →
Research ArticlePurification, Serology and Prevalence of Broad bean stain Comovirus (BBSV) and Cowpea Aphid Borne Mosaic Potyvirus (CABMV)
S. A. Sidaros, S. A. EL-Kewey, Eman A. H. khattab, M. M. ELsharkawy
BBSV and CABMV were purified from faba bean and cowpea plants, respectively using two different methods. The UV absorption spectrum had maxima and minima at 260 and245 nm, respectively for the two viruses. The absorbance ratios of A and A260/280 of the combine…
5 Jan 2024
Research ArticleIsolation and Characterization of a Pseudomonas Putida Bacteriophages
Mayada A. Abd Elgalell, B.A Othman, Th. Radwan, Amal S.M. Abo-Sinna
Two phage isolates for Pseudomonas putida were isolated from soil cultivated with potato by the enrichment culture technique and signed as PPI and PP2. The phages were propagated and purified by polyethylene glycol, 6000-sodium dextran sulfate 500 two phase sy…
5 Jan 2024
Research ArticleIsolation and Characterization of a Pseudomonas aeruginosa Bacteriophage from Agriculture Drain Water
Amina A. II, M.K. Amin
Five bacteriophages infecting Pseudomonas aeruginosa strain were isolated from agricultural drain water of Zagazig city. One of them designated as PAI has been characterized. The ability of phage PAI to form plaques with strain PAOI was examined. Two kinds of…
5 Jan 2024
Research ArticleComparative Study on Two Thrips Transmitted Viruses Tomato spotted wilt virus (TSWV) and Iris yellow spot virus (IYSV) as a Tospovirus
Manal A. El-Shazly, A. s. 2 Abdel Wahab, Salwa N. Zein
Biological, biochemical and serological comparison between Tomato spotted wilt virus (TSWV) and Iris yellow spot virus (IYSV) a Tospovirus were studied These two viruses were isolated from Vinca rosae (L). G. Don and onion (Allium cepa L.), respectively. The o…
5 Jan 2024
ArticlesBiological and Molecular Characterizations for Identification of a Phytoplasma Associated With Lemon Witches’-Broom in Egypt
Rasha M. Mahrous, Ahmed K. El-Attar, Ahlam A. AlWatban, Sohair I. EL-Afifi, Nagwa M. Aref
Background: Phytoplasma Associated with Witches' broom disease (WB) is a very serious disease of citrus with a major limiting factor for lime production. Symptoms of Witches' broom were observed on limes in AL-Qalyubia governorate, Egypt. This study identified…
18 Aug 2021
ArticlesGenetic characterization of rabbit hemorrhagic disease virus from naturallyinfected rabbits in Sharkia governorate, Egypt
Naglaa F.S. Awad, Gamelat K.F. Kotb
Background: Since the first introduction of rabbit hemorrhagic disease virus (RHDV) in 1991, Egyptian authorities started a national vaccination program against the disease. However, RHDV clinical cases are still being reported. Objectives: This study aimed to…
18 Aug 2021
ArticlesDifferent culturing systems of IBV-Eg-F170-2015 strain of Egypt variant 2 infectious bronchitis virus
Ahmed A. Azab, Wesam H. Mady, Ali Zanaty, Mahmoud Samir
Background: Infectious bronchitis virus (IBV) is an acute contagious disease cause severe economic losses in poultry production worldwide. Virus isolation is the golden standard for IBV diagnosis so the aim of this study was comparing the different biological…
18 Aug 2021
ArticlesIdentification, characterization and ultrastructure aspects of Alfalfa mosaic virus infecting potato (Solanum tuberosum L.) in Egypt
Maha A. El-Abhar, Moustafa A. Elkady, Khaled M. Ghanem, Hussieny A. Bosila
Background: Alfalfa mosaic virus (AMV) is only virus in the genus Alfamovirus and has very wide host range among weed and crop plants which produces a variety of symptoms. It can cause problems in potato in some regions where vectors easily move into potato fi…
18 Aug 2021
Research ArticleCytological and Molecular Studies of an Egyptian Isolate of Carnation Vein Mottle Potyvirus
Amal Abou El-Ela A, M. A. Amer, Eman A. H. Khatab
Carnation vein mottle virus CarVMV was isolated from infected carnation plants and identified by host-range, serological detection and maintained on Carnation (Dianthus caryophyllus L.) and / or Gompherena globosa. Morphological studies of CarVMV were conducte…
5 Jan 2024
ArticlesSensitivity of Real Time –PCR in comparison with conventional methods for detection of AI virus
Lamiaa M. Omar, Mohamed A. Abdrabo, Dali M. Omar, Nermeen A. Marden
Background: Avian influenza virus (AIV) infection, an important but difficult pathogen to control in poultry. Vaccination might reduce the risk of bird infection virus transmission by reducing virus shedding. Challenge of vaccinated birds with AI vaccines unde…
18 Aug 2021
ArticlesMolecular characterization of foot and mouth disease virus (O-EA3) isolated during 2016 outbreak in Egypt
Muhammad G. Khodary, Ayman H. El-Deeb, Mohamed M. Emara, Othman E. Othman, Hussein A. Hussein
Background: In January 2016, several cases of clinical signs and mortalities suggested foot and mouth disease (FMD) outbreaks among cattle and buffalo herds in Egypt. This study aimed to investigate the contribution of Foot and Mouth Disease Virus (FMDV) and c…
18 Aug 2021
ArticlesMolecular detection and phylogenetic analysis of sheep pox virus in El Menofiya Governorate
Ashraf S. Khameis, lamya F. Atteya, Ayman H. Mansour, Heba A. Abdelhady, Ashraf A. Saad
Background: Sheep pox virus is a member of the Capripoxvirus genus of the Poxviridae family, which affect sheep and causes significant economic losses in El Menofiya Governorate. Objectives: The present study was applied for isolation and molecular identificat…
18 Aug 2021
ArticlesEffect of methanolic-extracted green tea on hepatitis A virus and its usage in clearing the virus from drinking Water
Dina N. Abd-Elshafy
Background: Hepatitis A virus (HAV) causes tens of millions of human infection annually worldwide. It usually spreads by the fecal-oral route and is transmitted from person-to-person through contaminated food or water. A number of travelers arriving from Egypt…
18 Aug 2021
Research ArticleIdentification and Elimination Treatment of Prunus necrotic Ring Spot Virus (PNRSV) in Rose
Amal Abou El-Ela
Prunus necrotic ring spot Ilarvirus (PNRSV), was isolated from rose shrubs during the survey of rose plantations in Orman Garden, showing Ilarvirus-like symptoms. To identify the causal virus, the plants were tested by enzyme-linked immunosorbent assay using a…
5 Jan 2024
