Articles

Sequencing and Phylogenetic Analysis of GPCR, RPO30, P32 and EEV Glycoprotein Genes of Lumpy Skin Disease Virus Recent Isolates in Egypt

Moustafa A. Zaghloul, Mohamed F. Azooz, Saleh E. Ali, Heba M. Soliman, Maha M. Sayed, Mohamed H. Kafafy et al.

Lumpy skin disease is an emerging viral illness of ruminants that is highly contagious and economically damaging. The goal of this study is to perform sequencing and phylogenetic analysis of GPCR, RPO30, P32 and EEV glycoprotein genes of Lumpy Skin Disease Vir…

Cocktail Phage Therapy for Bacteria Contaminating Meat in Egypt

Amany M. Reyad, Aya Maher Rabie, Reda Mohamed Taha, Khalid El-Dougdoug

Bacteriophages have crucial advantages over conventional methods of controlling pathogenic bacteria in which they have a high ability to self-reproduce, host specificity, and develop with their bacterial hosts. The most common pathogenic bacteria were isolated…

Improving the Phytosanitary of Fig Plants Infected with Fig Latent Virus by Nanochitosan and Biomagic

Hanaa H.A. Gomaa, Dalia Y.A. Amin, Mona A. Ismail, Basma Hamdy, Khaled A. El-Dougdoug

Fig viruses have been found as naturally mixing infection in many countries in the world and Egypt. The present study aimed to evaluate the potential effect of nanochitosan and biomagic for treatment of the pathological effects related to virus infection in fi…

Molecular Characterization of Recent Field Isolate of Lumpy Skin Disease Virus Compared to the Vaccinal Strains

Akram A. Salama, Moustafa A. Zaghloul, Ahmed A. Zaghawa, Mohamed A. Nayel, Ahmed M. Elsafey, Mohamed F. Azooz et al.

The lumpy skin disease virus (LSDV), causes lumpy skin disease (LSD), is a transboundary viral disease of cattle that has a significantglobal economic impact. This study aims to identify the lumpy skin disease virus that circulate…

Effect of environmental factors on Peanut Mottle virus infection and population of insects on Lentil in Egypt

 Peanut Mottle Virus (PMoV) was isolated from Lentil cvs. Giza51. The infected indicator plants featured severe mosaic on Lentil cv. Giza51, mottling on cv.Giza9, mild mottle on cv. Giza 4, yellow mild mottling on cv.  Giza370. Seed transmission test…

Phylogenetic relationship of some Potyviridae members that found in Egypt.

Mervat M. Fath- Allah, Amal A. Ahmed, Hala A. Amin

Five different plant samples showing different Potyviridae symptoms were previous isolated and identified. All infected plant samples were examined using ELISA using against, number of potyviruses polyclonal antibodies. The ELISA results gave a positive reacti…

Characterization of Tobacco Rattle Tobravirus from Kaki (Diospyros Kaki)

Saiwa N. Zein

Kaki (Diospyros kaki) trees, showing vein banding. upward rolling of leaves and stunting of growth were observed in the farm Of South Tahrir. Isolation trials revealed that these symptoms were induced by Tobacco rattle virus. The virus was transmitted by mecha…

Identification of Sugarcane Mosaic Potyvirus Strains in Egypt

A.l. Abd El-Fattah, A.s. Saclik M.M. El-Kholi, I.A. Åbdcl-Hmnid, M.A. Madkour

Sugarcane mosaic pot.1Virus (SCMV) is the causal agent Of mosaic disease which is reported to be one of the most important viral diseases attacking sugarcane crop in Egypt. Therefore. the present work was designed to screen the SCMV strains in Egypt based on b…

Production of Polyclonal Antibodies against the Recombiant Citrus tristeza virus Coat Protein Expressed in Escherichia coli

Hala A. Amin, A. Barakat, A.A. Abou Zeid

Early diagnosis based on immunoenzymatic techniques requires significant amounts of immunoreagents. Hence, using directional cloning technology, a 38-KDa fusion protein containing a complete coat protein (CP) gene or Citrus tristeza virus (CTV) encoded by the…

Isolation and Identification of the Causal Virus Aucuba Disease in Egypt

A. S. Gamal El din, Sohair I. El-Afifi, A. S. Sadik, Nashwa M. A. Abd El Mohsen, H. M. Abdelmaksoud

The infected potato plants showed aucuba symptom characteristic to this virus (brilliant and extensive yellow mottle on lower and middle leaves reaches their tips, brown patches and sunken brown areas were frequently observed on the tubers surface) were used a…

Molecular Characterization of Potato Spindle Tuber Viroid Egyptian Isolate

E. K. Allam, Kh. A. El-Dougdoug, M. A. Amer, A. A. Abou-Zaid, S. M. Amin

Potato spindle tuber viroid (PSTVd) was detected in potato plants grown in Egypt using return-polyacrylamide gel electrophoresis (R-PAGE) and molecular techniques. The method involved the extraction of circular nucleic acids followed by electrophoretic analysi…

Purification and Serological Studies on Barley Stripe Mosaic Hordevirus

M.A.S. El-Kady, Om-Hashem M. El-Banna, E.A Salama, Salwa N. Zein

Three different procedures of purification were performed to purify a local isolate Of Barley stripe mosaic Hordevirus (BSMV). The 1st procedure was successful in producing relatively high yield of virus preparation 3.7 mg/100 g of barley leaves with adequate…

Characterization and Molecular Detection of Tomato spotted wilt Tospovirus Infecting Tomato in Egypt

Hayam S. Abdelkader, Gamalat M. Allam, T. A. Moustafa, M. El-Hanunady

Tomato spotted wilt virus was originally isolated from naturally infected tomato fruits. The virus infected a wide host range showing different symptoms varied from local necrosis, systemic necrosis, systemic mosaic yellowing, bronzing, or stunting. The virus…

Detection of Grapevine Fanleaf Virus Capsid Protein Gene by RT-PCR and DNA hybridization

E. K. Allam, B. A. Othman, Hayam S. Abdelkader, Noher A. Mahmoud

Grapevine fanleaf virus (GFLV) was isolated from symptomatic grapevine (Vitis vinifera L.) leaf samples obtained from El Kalubia Governorate. Three out or 25 samples were positive for GFLV by DAS-ELISA using specific polyclonal antibodies raised against purifi…

Immunocapture Polymerase Chain Reaction (IC-PCR) and Nucleic Acid Hybridzation Techniques for Detection of Spiroplasma citri

Om-Hashem M. El Banaa l, A.A. Abou Zeid, Fawzia I. Moursy, Azza, G. Farag

Spiroplasma citri Saglio et al is the type species of the genus spiroplasma. Spiralin gene is the gene responsible for spiralin which is the major protein of Spiroplasma citri (S. citri Saglio et al), the causal organism of citrus stubborn disease. RecA gene p…

Assessment of Hepatitis E Viral Infection and its Effect on Egyptian Pregnant Mothers

A. A- E. Abo-Elkhier, K. S. E. Abdel-Wahab, M. A. Ali, M. A-H. El-Sayed, N.A-K. Saleh, A. A. Atef

The current study was performed to determine the prevalence of HEV in Egyptian pregnant women. and to estimate the risk or prenatal transmission of the virus and its effect in infected pregnant women. 120 aborted women between 17-36 years old and 120 women age…

Nucleotide and Amino Acid Sequences Aompatison between Two Strains of barley Stripe Mosaic Hordeivirus (BSMV)

Om-Hashem M. EI-Banna, M. A. S El-Kady, E. A Salama, Salwa N. Zein

Nucleic acid sequence comparison between the Egyptian strain (Gl19) and the American strain (ND 18) of Barley Stripe Mosaic virus (BSMV) revealed a high degree of sequence similarity of γ RNAs comparing to ß RNAs of both strains. On the Other hand,…

Cloning, Expression and Nucleotide Sequence of Coat Protein Gene of an Egyptian Isolate of Potato Virus y Strain NTN Infecting Potato Plants

M. A. Amer, M. H. El-Hammady, H. M. Mazyad, A. A. Shalabyl, F. M. Abo-El-abbas

Reverse transcription- polymerase chain reaction (RT-PCR) amplified product of the expected size of 801 bp was cloned into the Pinpoint Xa-l protein expression vector. The accury of each PCR amplified PVY CP gene was tested by PCR, restriction analysis, and tr…

Molecular Cloning and Expression of the Coat Protein Gene of Plum Pox virus El-Amar Strain in E. coli

M. A. Abou El- Nasr, Kh. A. Dougdoug, Hayam S. Abdelkader, Rehab A. Dawoud

Plum pox potyvirus (PPV), the causal agent of Sharka disease of Prunus inflicts severe crop losses in affected El-Amar area. The virus isolated from EL-Amar apricot trees, was propagated on apricot healthy seedlings. Degenerated oligonucleotide primers were de…

Biological and Molecular Characterization of Different Isolates of Potato Virus Y (N Group)

A.Y. Ahmad, M. A. Amer, T. A. Mostafa, F. M. Abo El-Abbas, M. El-Hammady

Ten isolates of Potato virus Y (PVY) were collected from diseased potatoes grown at different locations in Egypt and some of their biological and molecular characters were studied using several techniques. Many different symptotypes were appeared on Chenopodiu…