Wan-Long Zhu* and Gao Wenrong
... both in liver and brown adipose tissue, suggesting that A. chevrieri was more sensitive to cold than that of photoperiod. Together, these data suggested that A. chevrieri mainly depend on increasing thermogenic capacity to cope with cold or winter condition....
Maoying Zhu, Yuntao Ji, Xiaoyu Wang, Junying Pu and Changqing Qu*
...of cryopreserved porcine adipose-derived stem cells. Four different combinations of cryoprotectants with and without hydrogen gas (H) (purified H2 was dissolved into normal cryopreservation solution for 2 hrs under 0.6 MPa were tested including the following groups: control (CK), 100µm GSH, 10µm fasudil hydrochloride (FH) and a combination of the two (GSH + FH). A solution of 10% (v/v) Me2SO + 20% FBS was used as the standard ...
Wenqiao Hui1, Jishun Tang1, Dejian Zhu1, Qian Ban2* and Sheng Chen1*
...al metabolic cue linking adipose and the onset of puberty. In the present study, we first assessed the morphological changes in adipose tissue at pre-puberty and puberty onset stages of goats, and then determined the role of leptin to activate signaling pathways which modulate the expression of hypothalamic genes involved in reproduction and metabolism. We found that leptin significantly upregulated the LEPR in the
Tania A. Shakoori1,*, Soumble Zulfiqar2, Khadija Irfan Khawaja3 and Faisal Masud4
..., AMPK, Akt, and mTOR in adipose tissue from mice treated with NaB versus placebo. Four groups of mice (n=27) mice were administered oral NaB, intraperitoneal (i.p) NaB, oral placebo or i.p placebo for 16 days. RNA was isolated from the epididymal fat pads and relative genetic expression of AMPK, Sirt1, mTOR and Akt was measured using real time PCR. Oral NaB group showed decreased genetic expression of AMPK (0.04 fold, p<0.001), mTOR (0.30 fold, p=0.04), Ak...
Mian Sajjad Ahmad1, Somia Iqtadar1, Sami Ullah Mumtaz1*, Zafar Niaz1, Iqra Waheed2 and Sajid Abaidullah1
...s a state of excess body adipose tissue. Impaired glucose tolerance is a risk factor for developing diabetes Mellitus & is associated with hyperglycemia and insulin resistance. To determine the frequency of impaired glucose tolerance in obese patients. This Cross Sectional study was carried out in North Medical Ward, King Edward Medical University/ Mayo Hospital Lahore for 6 months i.e. January to June 2014. Total 160 obese patients visiting outpatient dep...
Selçuk Kaplan
...h is highly expressed in adipose tissue. Leptin is one of the most significant candidate gene marker for MAS studies. Therefore, bubaline leptin gene exon 2, part of the intron 1 and intron 2 region were amplified and sequenced to identify nucleotide variations in Anatolian water buffaloes. Sequence analysis were revealed seven polymorphic site (G1072A, T1081C, T1131G, T1143C, T1145G, T1151G and C1221T) and one monomorphic site (C1071T) in Anatolian water buff...
Guifen Liu1,2, Xiaomu Liu1,2, Khuram Shahzad3, Wei You1,2, Juan J. Loor3,* and Fachun Wan1,2,4,*
...
The accumulation of adipose tissue is largely influenced by the genetic background of cattle. Therefore, we analyzed the transcriptome changes within adipose tissue in beef cattle at different stages of growth. Microarray analysis of data at 12 vs 18 months, 12 vs 24 months, 12 vs 30 months, 18 vs 24 months, 18 vs 30 months and 24 vs 30 months uncovered a total of 10625 differentially expressed genes (P ≤ 0.05; no fo...
Larysa Kladnytska1, Anatoliy Mazurkevych1, Natalia Bezdieniezhnykh2, Oleg Melnyc1, Sergiy Velychko3, Mykola Malyuk1, Vasyl Danilov1, Yuriy Kharkevych1 and Magdalena Gryzinska4*
...ymal stem cells from dog adipose tissue during in vitro cultivation. Materials and Methods: Mesenchymal stem cells were obtained from dog adipose tissue by explant method. The cells were cultured in CO2 incubator by standard procedure. Expression of cytoplasmic and membrane proteins on dog stem cells from fat tissue at the IVth and Xth passages was examined by immunohistochemical method using...
Yan-Guo Han, Jia-Yuan Wu, Ri-Su Na, Wei-Jiang Si, Yu-Qin Han, Yan Zeng, Yong-Ju Zhao and Yong-Fu Huang*
...id in waist subcutaneous adipose tissue were detected using gas chromatography. Immunisation of oral and plasmid-injected KISS1 DNA vaccines induced strong antibody response, significantly suppressed testosterone secretion and reduced scrotal circumference and testis weight. It also significantly reduced the concentrations of 4-methyloctanoic acid and 4-methylnonanoic acid in adipose tissue. These findings indicated t...
Q. Sun1, Q. Liu1, R. Di1, Y. Wang1, S. Gan2, S. Liu2, X. Wang1, W. Hu1, X. Cao1, Zh. Pan1, X. Guo1, Y. Yang3, H.E. Rushdi4* and M. Chu1*
...els in both subcutaneous adipose and tail fat tissues were significantly (p<0.01) higher than the other tissues analyzed. Levels of THRSP expression in liver, subcutaneous adipose and tail fat tissues in Tan sheep (thin-tailed breed) were significantly (p<0.01) higher than that in Altay sheep (fat-tailed breed). The results obtained might expand our understanding of the important regulating role of THRSP ...
Ramazan Erdogan1*, Mikail Tel2, Vedat Cinar2 and Ragip Pala2
...ha;2-glycoprotein (ZAG), adipose triglyceride lipase (ATGL), glucose transporter-4 (GLUT-4) and insulin receptor substrate-1 (IRS-1) in adipose tissue of rats fed on zinc picolinate supplement diet. A total of 42 male 8-week-old Wistar albino rats were randomly divided into 6 groups, each of seven; Control (C), Zinc picolinate (ZP), Chronic exercise (CE), Chronic exercise+Zin picolinate (CE+ZP), Acute exercise (AE), Acute ex...
Sen Liu1,2, Yue Zhu2, Haixia Leng2, Ying Wang1, Xiangju Yin1 and Yajun Zhao1*
...chnique, including brown adipose tissue (BAT), brain (B), heart (H), kidney (K), liver (L), and muscle (M) tissues. An average of 67.0% CCGG loci was methylated in each tissue. There were significant methylation differences among tissues in full-methylation (p < 0.001) and total methylation (p = 0.001). Moreover, various unique methylated loci (15–59) were also detected. Non-negligible level of hemi-methylation (31.4%) existed, indicatin...
HuaBin Zhang1,2, YaSen Li1, Tao Pan1, Peng Yan1, En Li1, Hui Xue1 and XiaoBing Wu1,*
...n the adipocyte of white adipose tissue, the gastric gland of the stomach, the lamina propria of the intestine and the interstitial cell and seminiferous tubule of testis, leptin receptor staining was detected in the adipocyte of white adipose tissue, the gastric gland and submucosa of the stomach, the submucosa, muscular layer and intestine villi of the intestine, the granulose cell and follicular membrane cell of the ovary...
Dong-Min Hou1, Ting Jia2, Chun-yan Liu1, Zheng-Kun Wang1 and Wan-Long Zhu1*
...daptive changes of white adipose tissue (WAT) in Tupaia belangeri under food restriction (FR, fed 70% of ad libitum food intake) and refeeding (Re), body mass, food intake, resting metabolic rate (RMR), morphology, the positive expressions of uncoupling protein 1 (UCP1) and Cd137, and the relative expressions of PR domain containing 16 (PRDM16), bone morphogenetic proteins 7 (BMP7), peroxisome proliferator-activated receptor α (PPARα)...
Dong-Min Hou1, Ting Jia2, Hui-Juan Wang1, Zheng-Kun Wang1 and Wan-Long Zhu1*
...ogenic function of brown adipose tissue (BAT) was known to be markedly elevated when animals were exposed to the cold. In the present study, transcriptome sequencing of BAT in Tupaia belangeri between control and cold acclimation group were carried out by Illumina novaseq 6000 platform. Then the pathways and key genes related to lipid metabolism and energy metabolism were screened out by bioinformatics analysis. The results showed that there were significant d...
Abdul Aziz1,2, Hamad Bin Rashid1*, Muhammad Arif Khan1, Asim Khalid Mahmood1, Ayesha Hassan1, Hamid Akbar1, Sadaf Imran1, Naveed Hussain1, Muhammad Umar1, Muhammad Asif1, Sajjad Javaid1 and Mamoona Chaudhry3
...rization of SVF from the adipose tissue of rabbits. Twelve healthy rabbits having body weight of 1000-1500g and of the same age were included in this study. SVF was prepared from bilateral inguinal fat that was harvested under general anesthesia. The harvested fat was enzymatically digested with 0.1% collagenase-I. It was then centrifuged and the SVF pellet was collected. The pellet was characterized through total viable cell count, cell per gram and percentag...
R. H. Jafri Dr., K.M. Saleem, R. Iqbal and Qaisra Fazal
...howed that the nuclei of adipose tissues, tracheal membrane, dermal cells and blood cells developed polyhedral bodies within a week after infection. Nuclear polyhedrosis virus multiplied rapidly and finally occupied the whole cell. Due to rapid increase in number of nuclear polyhedrosis virus, the cells and finally all the tissues disintegrated, resulting in death of the individual. Nuclear polyhecrosis virus infection in silkworm caused heavy losses to poor S...
Desak Nyoman Dewi Indira Laksmi1*, I. Gusti Ngurah Bagus Trilaksana1, I. Wayan Sukernayasa1, Nyoman Oka Widiarta2, I. Wayan Nico Fajar Gunawan1, I Made Merdana3
...nergy reserves stored in adipose tissue increases when there is a negative energy balance after childbirth. Leptin is a hormone secreted by adipose tissue that plays a role in conveying metabolic information to the hypothalamic-pituitary axis of the ovary. Therefore, this study aims to show that leptin can increase the estrus intensity and the appearance time of postpartum estrus in primiparous cattle. This is a true experim...
Suroor Hafedth Mohammed, Tahreer Mohammed Al-Thuwaini*
... of litter size with the adipose tissue biomarkers in Awassi ewes. In the current study, 100 sexually mature, not pregnant or lactating, and healthy ewes between the ages of 3 and 4 years were evaluated. The live body weight and age of each animal were determined. Serum was collected to measure leptin and visfatin hormones using ELISA kits made by SunLong Biotech. This study revealed that litter size significantly (P ≤ 0.05) influences the live body weight ...
Tamadhur H. Hussein, Layth H. Merzah, Tahreer M. Al-Thuwaini*
...produced and secreted by adipose tissue, including leptin and visfatin. Adipokines increase energy levels and stimulate the production of essential reproductive hormones to enhance reproductive activity in livestock. Therefore, this study examined the relationship between litter size and adipokines in Hamadani ewes. This study examined 140 ewes aged between 3 and 4 years, which were sexually mature, not pregnant or lactating, and healthy. Live body weight, age...
Sana Eijaz, Asmat Salim* and Mohammad Anwar Waqar
...s in skeletal muscle and adipose tissue including insulin receptor (INSR), insulin receptor substrate-1 (IRS-1), protein tyrosine phosphatase-1B (PTP-1B), phosphoinositide 3-kinase (PI3-K), protein kinase B (PKB), and phosphoinositide-dependent protein kinase-1 (PDK-1) and protein kinase C-theta (PKC-θ) genes and on different adipokines level. Wister rats were used to develop type 2 diabetes using high fat diet and streptozotocin and treated with the ext...
Khalid Hadi Kadhim1*, Iman Mousa Khaleel2, Firas Abbas Hussen3
...e connective tissues and adipose tissues. Whereas, the tail region was contained a large amount of skeletal muscle fibers. Furthermore, sweat and sebaceous glands had a positive reaction to Periodic Acid-Schiff staining, while with alcian blue stain appeared sebaceous glands only. From this study concluded the layers thickness of Gazella subgutturosa skin were differed according to the body regions and gender. As well as, the skin acted as a protective barrier...
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