Manzoor Hussain*, Miloslav Zouhar and Pavel Rysanek
...ere also improved due to antagonistic relationship of fungi and nematodes in soil....
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...nd 2000 eggs) mixed with antagonistic fungal conidia levels (1×103, 1×104, 1×105, 1×106) were used during the experiments. The roots were stained with Phloxine B, the egg-masses were quantified, the root systems were rated for galling, and the egg masses were measured on a 0 to 5 scale, where 0 = no gall or egg masses, 1 = 1-2, 2 = 3-10, 3 = 11-30, 4 = 31-100, and 5 = >100 galls or egg masses per root system. The nematode reproductio...
Sana Irshad Khan1*, Zafar Iqbal2, Hamid Ullah Shah2 and Shaukat Hussain3
Honnur Basha, Vinaya Hemannavar, B.Ramanujam, R. Rangeshwaran and S.Sriram
Screening of chilli microflora and other biocontrol agents for their antagonistic effects on Colletotrichum spp. infecting chillies
...ts were tested for their antagonistic effect on Colletotrichum gloeosporioides and C. capsici by dual culture test. Among the isolates of chilli microflora tested, Aspergillus flavus showed 70.2% inhibition of C. gloeosporioides and 54.9% inhibition of C. capsici.Among the isolates of Trichoderma sp. tested, T.virens (Tvs-KSD isolate) and T. pseudokoningii (Tpk-1) showed highest percent inhibition of C. gloeosporioides (69.7%) and T. viride (Tv-5 isolate) show...
Bushra Niaz1,*, Tahir Zahoor1, Muhammad Atif Randhawa1 and Amer Jamil2
Muhammad Arslan Khan1,*, Sajid Aleem Khan1, Imran-ul-haq1 and Rashad Waseem Khan2
Ghulam Muhae-ud-Din1,4, Anam Moosa1, Umer Farooq Ghummen1, Muhammad Jabran1, Amjad Abbas1, Muhammad Naveed2, Abdul Jabbar1 and Muhammad Amjad Ali1,3,*
...ould be managed by using antagonistic, growth promoting endophytic bacteria. ...
Baharullah Khattak1*, Saifullah2, Shaukat Hussain2, Musharraf Ahmad2, Asad Ali2, Mohammad Junaid2, Ijaz Ahmad Khan3, Taj Ali Khan1 and Mubbashir Hussain1
...dogyne javanica. Anti-J2 antagonistic test divided the isolates into 5 antagonistic groups or AGs. AG1 was observed as the most antagonistic group, causing an average of 66.6% J2 mortality. Genetic variability among these isolates, was studied at molecular level by randomly amplified polymorphic DNA procedure. A selected set of three random primers was used for this purpose, which resulted...
Tariq Mukhtar
...ive effectiveness of two antagonistic fungi viz. Trichoderma harzianum and T. viride were evaluated against Meloidogyne incognita on tomato. The application of T. harzianum and T. viride significantly increased shoot weight and decreased root weight of tomato in a dose dependent manner. Doses of 8×103 and 1×104 cfu/g of soil showed maximum increase in shoot weight and decrease in root weight....
Javed Asghar Tariq1*, Bashir Ahmed2, Manzoor Ali Abro2, Muhammad Ismail1, Muhammad Usman Asif1 and Raza Muhammad1
...rowth as well as to have antagonistic effect against another pathogenic microflora. Current studies were designed with the aim to search out such type of antagonistic bacteria from rice crop. For this purpose, soil was collected from rice growing areas of Tando Jam. The isolation from rhizospheric soil was done by dilution plate technique. Among isolated isolates, 6 representative colonies were purified, multiplied and chara...
Jam Nazeer Ahmad1,2*, Rashid Mushtaq1, Samina Jam Nazeer Ahmad1,2*Mubasher Ahmad Malik1, Mujahid Manzoor1, Muhammad Tahir2, Zubair Aslam4, Sumaira Maqsood5, Ishita Ahuja3 and Atle M. Bones3
...synergistic, additive or antagonistic effect of three sub-lethal dose rates (2 x 103, 4.5 x 103, and 6 x 103 PIB/Larva) of native isolated Nucleopolyhydrovirus (NPV) from Spodoptera litura and Spinosad (0.01 ppm) against 3rd and 4th instar larvae collected from three different geographical areas of Punjab (Pakistan). A difference in larval mortality, pupation, adult emergence and egg ecluson was obs...
Sana Aslam1, Wayne Thomas Shier2 and Imran Sajid1*
...n and to screen them for antagonistic activities against multi drug resistant pathogens and to characterize the bioactive metabolites produced by them. A total of seventy strains of actinomycetes were isolated from lake samples, out of them only forty bioactive strains were selected for further characterization on the basis of their preliminary antimicrobial activities. The isolated actinobacterial strains showed an optimum growth at alkaline pH (9.0-9.5). The...
Wajahat Azeem1,*, Tariq Mukhtar1 and Tooba Hamid2
...rma harzianum and an antagonistic plant, Azadirachta indica was tested against M. incognita on tomato. The antagonistic fungus and plant caused significant hatching inhibition and larval mortality of M. incognita. The hatching inhibition and mortality was the maximum at 100% concentrations of both the agents while the minimum inhibition and mortality was obtained at 25% concentration. No statistical ...
Asma Chaudhary1, Qurat-ul-Ain Ahmad1, Afia Muhammad Akram1, Sadia Roshan2 and Javed Iqbal Qazi3*
Muhammad Junaid Yousaf 1, Farhad Ali2*and Fawad Ali2
...e seedlings revealed the antagonistic effects which increased with elevated concentrations of salt stress. The experiment was kept for 5 weeks and the chlorophyll content, relative water contents (RWC), leaf water loss (WL), and Electrolytic leakage (EL) were measured, the seedlings showed adaptation to the salt stress at the expense of growth rate as high seedling death occurred at 1.2% and 2% treated pots. However, the high growth observed in control pots ha...
Ramy S. Yehia1,3, Essam A. Shaalan2* and Hashem M. Al-Sheikh1
...t fungi mixture produced antagonistic effect. The development of M. domestica larvae was affected by sublethal dose from fungi. In conclusion, M. anisopliae is more efficient than B. bassiana and could be easily mixed with essential oils to either enhance larvicidal activity or utilize in integrated pest management. Furthermore, research on field evaluation and deleterious effects of environmental conditions on fungi capacity is required....
Seun Eui Kim1,2*, Myoung-Hoon Lee1, Hye Myoung Jang2, Garam Park2, Wan-Taek Im3, Gwang Joo Jeon2,4*
...ing that CK properly and antagonistically works on anticancer activity. The expression of angiogenesis-related gene of VEGF unexpectedly was increased. In conclusion, this study confirmed that ginsenoside CK is a new anticancer candidate with excellent anticancer activity inducing cell cycle arrest, apoptosis and in some extent cell migration/metastasis inhibition for neuroblastoma.
Keywords | Neuroblastoma, Compound K , Anticancer
...
Eman Alsayed Hammad1* and Mahmoud Mohamed Hassanin Hasanin2
S. Miheret1, A. Seid2† and N. Hailu3
...pping of tomato with the antagonistic marigold followed by tomato with garlic intercropping was found highly reducing root galls, egg-mass and J2 per plant as compared to the inoculated tomato-sole cropping and with other antagonistic plants. Further research is needed to evaluate the effectiveness of the antagonistic plants in the management of root-knot nematodes under farmer’s fie...
M. T. Javeed†, A. S. Al-Hazmi and Y. Y. Molan
A.W. Amin† and A.W. Mona*
...pp.) as
root antagonistic plant and Rugby as a nematicide (two formulation, Rugby 10G and Rugby 20L) for control of
Meloidogyne incognita cucumber root-knot were evaluated in nematode naturally infested soil under greenhouse
conditions in two successive spring seasons (2011 and 2012). Cucumber, Cucumis sativus var. Sinai was planted as
a scion. Results indicate that cucumber plants gr...
Tabark F Ahmed, Nibras Z. Yahya*
...h erythromycin exhibited antagonistic effects. Furthermore, time-kill analysis showed that ASCORBIC/CIP combination exhibited highest synergistic and bactericidal effect. It was determined that in vitro study against E. coli O157 in the treatment of urinary tract infection, the combination of ascorbic acid and ciprofloxacin was both more potent and safer than the combination of ascorbic acid and erythromycin. From this study concluded the combination of ciprof...
Chen Zhao, Lulu Ding, Ying Ye, Congying Kou, Haoran Xiao, Jing Zhu and Jicang Wang*
...gy in the kidney and the antagonistic effect of Nar on Cd are still unclear. In this study, SD rats were treated with Cd and/or Nar to investigate the protective effect of Nar on Cd-induced toxicity. The rats were treated for 4 weeks. The histopathological observation, oxidative stress index and autophagy-related gene expression level were detected of rat kidneys. The results showed that Cd caused the pathological changes of kidneys. And Cd also increased the ...
Ummul Firmani1,3, Rahmi Nurdiani2, Arning Wilujeng Ekawati2 and Happy Nursyam2*
... hemolytic activity, and antagonistic activity. The results showed that there were several types of bacteria found in the digestive tract of milkfish. One of these bacteria could produce an amylase enzyme with an amylolytic index of 5.16, identified as Bacillus paramycoides. This bacteria also synergized with other bacteria and did not have hemolytic activity on blood agar media. The results of the antagonistic test based on...
Wei Wan1, Xiao-Li Wang1, Shu-Juan Wang2, Qin Si2 and Li-Qing Duan1*
...toxicity, synergistic or antagonistic effects on adult P. sinica. The modified residual film method was used to test the toxicity of individual and binary mixture of these monoterpenoids against adult P. sinica. The effects of these monoterpenoids on acetylcholinesterase (AChE) and glutathione S-transferase (GST) activities in P. sinica were assessed in vitro. Correlation between numbers of synergistic or antagonistic binary...
SEHRISH MUSHTAQ1, MUHAMMAD SHAFIQ1, MUHAMMAD ASHFAQ*1 FAIZA KHAN1, SOHAIB AFZAAL1, UMMAD HUSSAIN1, MUBASSHIR HUSSAIN1, RASHID MUKHTAR BALAL2 & MUHAMMAD SALEEM HAIDER1
...ematic bacteriology. The antagonistic potential of bacterial endophytes to A. solani was explored using the agar absorption method. This study showed the antifungal potential of Pantoea sp. (35.66%), Ensifer adhaerens (35.33%), Citrobacter diversus (33.03%), and Azotobacter nigricans (31.56%) to check the growth of pathogenic fungi compared to controls. Aureobacterium liquifaciens (27.66%), Acinetobacter sp. (25.66%), Bordetella pertussis (26.63%) also showed ...
Riam Yousfe Muttair
...tors demonstrate various antagonistic activity against multiple immune checkpoints as well as resistance mechanisms at the same time. It follows from here that these can offer a promising prospect of outcomes improvement as well minimization of off-target effects. These studies deliver the information about a growth of immunotherapy, and the production of the tumor microenvironment in the fight against cancer. ...
Jumiati Asis1, Nur Aainaa Hasbullah1, Mohamadu Boyie Jalloh1, Noor Khairani Mohamad Basri1, Palanivell Perumal2, Peter Mojiun2 and Mohd. Rashid Mohd. Rakib1*
...s) that have significant antagonistic activity against various fungal phytopathogens. On the other hand, Ganoderma boninense has been identified as the phytopathogen causing basal stem rot (BSR), a devastating disease in oil palm crop (Elaeis guineensis). In this study, the in vitro and in planta inhibition of G. boninense using Trichoderma isolates were evaluated. A total of 20 Trichoderma isolates were collect...
R. Umamaheswari*, P. Prabu, M.S. Rao, B.M. Kavya and G. N. Grace
...agents demonstrate their antagonistic action against a multitude of phytopathogenic microbes and nematodes. Bacillus amyloliquefaciens IIHR BA2 (Indian Institute of Horticultural Research, Bacillus amyloliquefaciens 2) is one such potential bacterial bioagent that demonstrated effective nematicidal activity against root knot nematode, Meloidogyne incognita, a major biotic constraint in successful crop production. This study aims to decipher the mechanism of ac...
Ahmed M.F.A
Volume 2, Issue 2 March and April 2018 Pages 37-47
...d plants. Many different antagonistic isolates (bioagents) i.e. Trichoderma album,
Abdelhak Rhouma 1 Ibtissem Ben Salem 1 Mahmoud M’Hamdi 2 Naima Boughalleb-M’Hamdi 1
Volume 2, Issue 5 September and October 2018 Pages 85-100
Ibtissem Ben Salem1; Yosra Abdelkhalek1; Houssem Nabli3; Neji Tarchoun2; Naima Boughalleb-M’Hamdi1*
Novel Research in Microbiology Journal (2019), 3(1): 232-242
...eed-borne pathogenic and antagonistic fungi were isolated from the same organic vegetable and spice seeds (carrot, fennel, broad bean, faba bean, and lettuce), and the interaction between them were studied as a part of a biocontrol assay. In vitro dual culture assay between each pathogen and the antagonists on PDA medium was made, which led to the inhibition of the pathogens mycelial growth. According to results generated for fennel seed-borne fungi, direct co...
Mahmoud Saber Kelany1*; Ehab Aly Beltagy2; Maha Abd El-Fattah Khalil3; Mohamed Ahmed El-Shenawy2; Wagih Abd El-Fattah El-Shouny3
Novel Research in Microbiology Journal (2019), 3(1): 258-270
...these isolates exhibited antagonistic activities against different bacterial pathogens. A promising bacterium isolated from Suez bay marine water was identified as Bacillus sp. MH20 using 16S rRNA sequencing, and deposited in GenBank with accession number KM374670. This isolate showed sequence similarity of 88% to B. sonorensis strain NBRC 101234. Well-cut diffusion assay was adopted to determine the in vitro antibacterial activity of the active compound produ...
Ahmed, M.F.A.1*; Shaheen, S.I.2; EL-Fiki, I.A.I3; Ahmed, M.S.M.4
Novel Research in Microbiology Journal (2022), 6(5): 1682-1699
...ant in vitro and in vivo antagonistic efficacies against certain soil-borne fungal pathogens, which cause root rot diseases of cantaloupe plants during the growing seasons of 2019/ 2020 and 2020/ 2021. In the in vitro assays, T. harzianum caused a significant reduction of the pathogens' radial mycelial growth, including Rhizoctona solani, Phytophthora spp., Sclerotium rolfsii and Fusarium oxysporum by 79.69 %, followed by T. viride (76.26 %), and T. album (70....
Sherin P. Mekhail1; Marwa S. Fouad2*
Novel Research in Microbiology Journal (2024), 8(2): 2393-2413
...aimed to investigate the antagonistic activity of Trichoderma harzianum against D. graminea. Pathogen was isolated from the naturally infected barely leaves and molecularly identified. The identified isolate was deposited in GenBank with an accession number of OR827023. In vitro dual culture assay showed a decrease in D. graminea radial growth with a growth inhibition percentage of 77.8 %. T. harzianum culture filtrate (TCF) (30×106 spore/ ml) was effici...
Belal Natey1; Ahmed M.M.A. Kasem1; Younes M. Rashad2*; Nageh Fathy Abo-Dahab1
Novel Research in Microbiology Journal (2024), 8(6): 2712-2733
...yptian governorates. The antagonistic activity of all isolated Trichoderma strains was screened against S. cepivora BYAN1 in vitro. Microscopic examination showed that isolate B3R12 (identified as T. afroharzianum B3R12) has the greatest mycoparasitic level. Moreover, this isolate showed high in vitro inhibitory effect on S. cepivora BYAN1 growth by the production of both volatile and nonvolatile antifungal metabolites, recording inhibition of 74.32 % and 71.6...
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