Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali*
...m containing only single auxin while combination of auxin and cytokine in MS and Chu’s N6 media produced 53.33 and 35.56% embryogenic calli, respectively. Inclusion of 5 mgL-1 silver nitrate in embryogenic medium enhanced the production of type II embryogenic calli by 54.90% compared to control treatment. Maximum regeneration (58.33%) was observed when embryogenic calli were placed on N6 medium supplemented with 60 gL-...
Arifa Khan1*, Arsalan Bashir1, Shazia Erum2, Jabar Zaman Khan Khatak1 and Aish Muhammad3
Effects of 6-Benzylaminopurine and Indole-3-acetic Acid on Growth and Root Development of Banana Explants in Micropropagation
...ndole-3-acetic acid (IAA auxin family) on growth and root induction of banana explants micro-propagated in vitro. Explants were obtained from young suckers of 8818-william, Pisang and Brazilian varieties. The explants were cultured in vitro containing MS media and different BAP and IAA combinations. The results showed banana varieties exhibited differences for the shoot and root development and also for the number of shoots and leaves. Wiallium-8818 hybrid gav...
Sajid Ali1, Abdul Basit1,3*, Abdul Mateen Khattak1, Syed Tanveer Shah1, Izhar Ullah2, Noor Alam Khan3, Imran Ahmad1, Kamran Rauf1, Salman Khan1, Irfan Ullah1 and Intizar Ahmad1
Managing the Growth and Flower Production of Zinnia (Zinnia elegans) through Benzyle Amino Purine (BAP) Application and Pinching
... cytokinins and decrease auxin concentrations flourishing the axillary buds. A research study on the growth and production of zinnia flowers through application of BAP (25, 50, 75 and 100 mg L-1) and pinching (2, 4 and 6 leaf stages), was carried out at The University of Agriculture Peshawar, Pakistan. Research analysis showed that zinnia plants treated with 100mg.L-1 and pinched at 6 leaves stage recorded maximum days to flowering, flower persistency, branche...
Mazhar Ullah and Mohammad Sayyar Khan*
...this research, different auxins [Dichlorophenoxy Acetic acid (2,4-D), Nephthalene Acetic Acid (NAA)], cytokinin [Benzyl Amino Purine (BAP)] either alone or in combination with each other and carbon sources [sucrose, glucose and fructose] with varying concentrations (2, 4 and 6%) were evaluated for their effect on the tissue culture of sugarcane variety-CP 77/400. For callus induction 2,4-D as an auxin was found to be most ef...
Muhammad Adeel Qureshi1*, Raza Ahmad2, Bilal Ahmed1, Tanzim Ullah Khan1 and Muhammad Yasin3
...(CIM), CIM1 with 2mg/lit auxin (2, 4-D) showed the best results. All the internodal explants placed on CIM1 produced 100% callus while CIM2 produced 70% callus. No callus was observed from leaves on CIM1 and CIM2. After callus induction, four different types of media were used for plant regeneration, having different concentrations of cytokinin (BAP and Zeatin) i.e. RZ1.5, RZ2, RB1 and RB2. The most efficient regeneration media was RZ2, having Zeatin 2mg/lit s...
Anwar ul Haq1, Maqsooda Parveen2, Shehzadi Saima3* and Khalid Masood Ahmad1*
...entration of appropriate auxins is used to promote effective rooting. Completely randomized design (CRD) is adopted and it consisted of five treatments including control and different concentration of both auxins viz. 1-Naphthalene acetic acid (50 mg/L,100 mg/L) and Indole-3-acetic acid (100 mg/L, 200 mg/L) on tip cuttings of chrysanthemum. In this experiment, 15 pots of 9-inch diameter were used which were filled with silt,...
Hanaa H.A.Gomaa1, A. F. Moustafal, Kh.A.El-Dougdoug2, A.A. Abou-Zeid3 and S. Y.M. Mahmoud4
...to plants contained less auxins and gibberellins, and the pattern of growth promotors and growth inhibitors was altered. Cytokinin was also inhibited as a result of virus infection. Examination of epidermal strips of infected potato plants revealed the presence of cytoplasmic inclusions. Many cytopathic effects showed in potato leaves such as presence cytoplasmic vesicles, abnormal appearance of grana and change of mitochondria....
Muhammad Afzal, Aqeela Mobeen Akhter and Masood Amjad
Q. N. Javeed, Rehana Perveen, Imtiazul Haq and Ihsan Elahi
...koog's. The effect of auxins, kinetin, coconut milk and casein hydrolysate was studied on induction and further growth of callus. Auxins were NAA (0.05, 0.1, 0.5 and 1.0 mg/1). Cytokinin used was BAP (1.0mg/1). Our results indicated that size of the explant, presence or absence of apical bud, controlled conditions of light and temperature are important factors in callus formation. A successful callus could only be raised ...
Pangesti Nugrahani1*, Hery Purnobasuki2, Arif Nur Muhammad Ansori3, Jatuporn Anuchai4 and Anugerah Dany Priyanto5,6
