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Assessment of Adaptation, Regeneration Capability and Fatty Acid Profiles of LiCl Adapted and Unadapted Cell Lines of Oryza sativa L. Cv. Swat-1

Qudsia Khalid, Safdar Hussain Shah*, Faiza Zaeem and Saad Hussain Shah 

Antibacterial Efficacy of Manuka Honey against New Delhi Metallo-β-Lactamase Producing Gram Negative Bacteria Isolated from Blood Cultures
Muhammad Usman Qamar1, 2, Sidrah Saleem1, Usman Arshad1, Muhammad Farhan Rasheed3, Hasan Ejaz4, Naveed Shahzad5 and Shah Jahan6,*
...stan. A total of five carbapenem resistant bacterial species; E. coli, K. pneumoniae, E. cloacae, A. baumannii and P. aeruginosa, were isolated and identified using VITEK 2 compact system. Carbapenamase and metallo-β-lactamase (MBL) production by these isolates was confirmed by modified Hodge’s test and disc potentiation method, respectively. The blaNDM-1genewas amplified by PCR and...
In Vitro Plant Regeneration from Coleoptilar Node of Maize Seedling: a New Tool to Bioengineer the Maize Rapidly

Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali* 

In-Vitro Propagation of Neurada procumbensl L (Chipri Booti): An Endangered Medicinal Plant from Cholistan Desert
Sumaira Zareen1, Syeda Sadaf Zahra2, Ayeza Mehmood3, Muhammad Asadullah4* and Aish Muhammad5

 

 Sadia Tehrim*, M. Yasin Mirza** and G. Mustafa Sajid*

COMPARATIVE STUDY OF DIFFERENT GROWTH REGULATORS FOR EFFICIENT PLANT REGENERATION IN GRAPES
Bad Bugs and No Drugs: Activity of Colistin as Waging War against Emerging Metallo-β-Lactamases Producing Pathogens
Sahar Naz1, Farhan Rasheed2, Muhammad Saeed3*, Shagufta Iram4 and Ambereen Anwar Imran5
...lines. Next day every carbapenem-resistant isolate were further processed for MβL detection by EDTA method, zone size of Carbapenem disc only and Carbapenem disc impregnated with EDTA was compared ( >7 mm increase MβL positive, 0-5 mm increase MβL-negative).
Results: Out of total 12126 samples, 35.9% (n=4361) were culture positive and only 40.5% (n=1770...
Prevalence of Metallo-β-Lactamase IMP and VIM Producing Gram Negative Bacteria in Different Hospitals of Lahore, Pakistan
Junaid Akhtar1,2, Sidrah Saleem1, Naveed Shahzad3, Abdul Waheed1, Iqra Jameel1, Farhan Rasheed4 and Shah Jahan5,*
...biotics predominantly carbapenems, are considered as last treatment option against multi-drug resistance GNR infections. However, the emergence of carbapenemases particularly metallo β-lactamases (MBLs) in bacteria have severely mitigated the efficiency of carbapenems. MBLs producing gram-negative bacteria have been reported from various hospital settings, worldwide. However, data is ...
Baohuoside I Inhibits Benzo(a)Pyrene-Induced Metastasis in A549 Lung Cancer Cells by Modulating STAT3 and EMT
Muhammad Khan1,*, Amara Maryam2, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1 and Yongming Li3,*
Efficient in Vitro Plant Regeneration Through Somatic Embryogenesis from Callus Induction Method for Brassica carinata

Rizwan Ullah Shah* and Iqbal Munir 

Isolation and Antimicrobial Susceptibility Paradigm of Carbapenem Resistant Metallo-Beta-Lactamase Producing Gram Negative Rods
Muhammad Hidayat Rasool1,*, Muhammad Zaheer1, Muhammad Farooque Hassan2, Muhammad Shafique1 and Muhammad Usman Qamar1
...ion cephalosporin and carbapenems is an issue of high importance. Present study was aimed to determine the frequency and antimicrobial susceptibility patterns of MBL producing carbapenem-resistant Gram-negative rods (GNRs) isolated from clinical specimens. A clinical descriptive study was conducted in Faisalabad, Pakistan from June 2016 to January 2017. A total of 152 clinical samples were processed according to standard mic...
Incidence of blaIMP and blaVIM Genes among Carbapenemase Producing Escherichia coli in Lahore, Pakistan
Shafqat Husnain Khan1, Shah Jahan2, Irshad Ahmad3,4, Sadeeq ur Rahman5,* and Tayyab ur Rehman4,*
...increasingly reported carbapenem resistance due to carbapenemases is highly worrisome. Literature regarding incidence of class B metallo-β-lactamases (MBLs) - producing E. coli has not yet judiciously been addressed. In this study, we investigated the occurrence of carbapenemase- and MBLs-producers among a collection of 100 carbapenem resista...
Genetic Transformation of Tobacco Serine Acetyltransferase 4 (NtSAT4) gene in Brassica napus L.

Hala Rajab1, Muhammad Sayyar Khan1*, Safdar Hussain Shah1 and Syed Mehar Ali Shah2 

In-Vitro-Management-of-Phytohormones-for-Micropropagation-of-Sugarcane

Amna Qazi1, Ghulam Shah Nizamani2*, Muhammad Tahir Khan2, Shafquat Yasmeen2, Shahla Karim Baloch1, Muharram Ali1, Imtiaz Ahmed Khan2, Sagheer Ahmad3, Muhammad Rashid Nizamani4 and Mohammad Aquil Siddiqui2 

Prevalence of New Delhi Metallo Beta-Lactamase (NDM) Producing Gram-Negative Bacteria from Different Tertiary Care Hospitals in Lahore, Pakistan
Farzana Rashid1, Rabia Masood1 and Mariam Faiz2*
...e="font-size: small;">Carbapenem resistance conferred by metallo-β-lactamase (MBL) has increasingly being reported worldwide among Enterobacteriaceae. In this study, 240 non repetitive gram negative clinical isolates were studied for antimicrobial resistance. Presence of bla NDM was determined by polymerase chain reaction. Among 240 strains, prevalence of MBL in K. pneumoniae, P. aeruginosa, E. coli and Acinetobacter Species was 26%, 52%, 27% and 5% respe...
Arifa Khan1*, Arsalan Bashir1, Shazia Erum2, Jabar Zaman Khan Khatak1 and Aish Muhammad3
Effects of 6-Benzylaminopurine and Indole-3-acetic Acid on Growth and Root Development of Banana Explants in Micropropagation

Sajid Ali1, Abdul Basit1,3*, Abdul Mateen Khattak1, Syed Tanveer Shah1, Izhar Ullah2, Noor Alam Khan3, Imran Ahmad1, Kamran Rauf1, Salman Khan1, Irfan Ullah1 and Intizar Ahmad1

Managing the Growth and Flower Production of Zinnia (Zinnia elegans) through Benzyle Amino Purine (BAP) Application and Pinching
Prevalence of Extended-spectrum β-lactamases in Escherichia coli Isolated from Chicken, Water and the Poultry Farm Workers
Sana Ilyas1, Muhammad Hidayat Rasool1,*, Muhammad Javed Arshed2, Muhammad Usman Qamar1 and Bilal Aslam1
... presence of ESBL and carbapenemase-producing genes using PCR. Of 392 samples, 219 ESBL positive E. coli were recovered and among these 156/213 (73.2%), 42/63 (66.6%) and 21/27 (77.7%) were from poultry, environmental water, and urine samples, respectively. The PCR results revealed that71.2% of blaCTX-M, 67.5% blaCTX-M -1 and 62.2% blaTEM producing isolates were recovered from poultry, 19.1% bla
Molecular Characterization of ESBL and Carbapenenemase Producing Salmonella spp. Isolated from Chicken and its Public Health Importance
Mona Kadry, Sara Mohamed Nader*, Esraa A. Elshafiee and Zeinab S. Ahmed
...al spread of ESBL and carbapenemase - producing Salmonella spp, which could pose a potential hazard to human and animal health. A total of 334 chicken meat samples, 197 eggshells and 160 human stool specimens were included in this study. The presence of salmonellae spp. was examined using bacteriological isolation and serological identification. PCR amplification of the ESBL and carbapenemase-encoding genes was performed. Th...
Emergence of armA Mediated Aminoglycoside Resistance in Multidrug-Resistant Acinetobacter baumannii in Pakistani Hospitals
Aqsa Shahid1, Saima Muzammil1, Farhan Rasheed2,Bilal Aslam1, Muhammad Akhtar Ali3, Syed Zohaib Haider4, Masroor Ellahi Babar4, Muhammad Saeed1, Umair Waqas1 and Mohsin Khurshid1,*
...ce to cephalosporins, carbapenems, aminoglycosides, and fluoroquinolones whereas colistin and tigecycline were the most active antimicrobial agents against A. baumannii as none of the isolates was found resistant to these two drugs. Moreover, the armA was found in 25% (n=37) of A. baumannii isolates which showed a high level of resistance to aminoglycosides i.e., amikacin; ≥256µg/ml (Breakpoints; ≥64µg/ml) and gentamicin;...
Effects of Carbon Sources and Growth Regulators on the Tissue Culture of Sugarcane

Mazhar Ullah and Mohammad Sayyar Khan*

In-Vitro Propagation for Conserving the Gems of the Hills Caralluma tuberculata to Rescue the Valuable Endangered Medicinally Important Plant
Muhammad Taqi1, Shazia Erum2, Shamaila Rasheed2, Sadar Uddin Siddiqui2 and Shakeel Ahmad Jatoi2*
Immune and Growth Response of Indigenous Pedi Goats Vaccinated with Blanthrax to an Inclusion of Moringa oleifera (Drumstick Tree) in Cenchrus ciliaris (Buffel Grass) Hay-Based Diet

Lungile Gumede1,2, Thobela L. Tyasi1, Teedzai Chitura1*, Khanyisile R. Mbatha3 

Prevalence of New Delhi Metallo-β-Lactamase-1 (blaNDM-1) Gene in Children from Tertiary Care Hospital of Pakistan

Farheen Aslam*, Hira Lodhi, Rasheeda Bashir, Faiza Saleem and Shagufta Naz

...text-align: justify;">Carbapenems are hydrolyzed by carbapanamase, present in the bacteria, which is a growing clinical threats. bla Ndm gene encodes for new Delhi metallo-beta lactamase, which can hydrolyze all types of beta-lactams. The objective of the study was to screen multiple drug resistant strains of bacteria for New delhi-metallo-beta-lactamase (bla-Ndm1) gene. Blood samples (5ml) of children suffering from differe...
Effect of Growth Regulators on Callus Induction and Plant Regeneration in Potato (Solanum tuberosum L.) Explants

Muhammad Adeel Qureshi1*, Raza Ahmad2, Bilal Ahmed1, Tanzim Ullah Khan1 and Muhammad Yasin3

Vegetative Growth Characteristics and Flower Sex Expression of Cucumis sativus Affected by Exogenous Application of Plant Growth Regulators

Getulio A. Barcenas Jr.1* and Luisa Marie I. Barcenas2 

Elimination of Grapevine Fan Leaf Virus (GFLV) and Rapevine Leaf Roll-Associated Virus-1 (GLRaV-1) from Infected Grapevine Plants using Meristem Tip Culture

Sahar, A. Youssef t ; M.M.A. Al-Dhaher 2 and A.A. Shalaby 

Evolution of super-drug resistant microbial strains: mechanisms and strategies for containment

SHEIKH AJAZ RASOOL1, MUHAMMAD SALMAN RASOOL2 & MUNAZZA AJAZ3

...actamases (ESBLs) and Carbapenemases. Drug resistance in viral entities has also posed challenge for public health programs. About 20% people die of viral hepatitis in one of Pakistan provinces. A counter malarial acrine drug is being tried against proteinaceous infectious particles (causing many transmissible neuro-diseases). These epigenetical agents have been a source of concern for our planet regarding food safety issues (e.g. infected meat). No doubt, man...
Assessment of plants producing economically valued non-timber products in Ukpom community forest, Akwa Ibom state, Nigeria
Enefiok S. Udo1, Opeyemi Olajide1 and Eyo A. Udoh2
Biodiversity conservation status in Pakistan - A review
Maqsood Anwar1, Abdul Wahid Jasra2 and I. Ahmad3
Propagation of Mulberry, Morus alba through a single node culture
Muhammad Afzal and Aqeela Mobeen Akhter
Propagation of Leucaena leucocephala through tissue culture
Muhammad Afzal, Muhammad Mushtaque, Aqeela Mubeen Akhtar, Nighat Chughtai and Shaheena Ramzan
Differentiation and anatomical changes in Callus cultures of Rauwolfia serpentine BENTH
Rehana Perveen, Mehmood Akram and Ihsan Ilahi
Propagation of Juniperus polycarpos C. Koch through tissue culture I. Induction of Callus
Q. N. Javeed, Rehana Perveen, Imtiazul Haq and Ihsan Elahi
Emergence of Carbapenem Resistant Escherichia coli Isolated from Human and Companion Animals Associated with Urinary Tract Infection

Ashraf Samir Hakim1*, Doaa Diab Khalaf1, Engy Farahat1, Mohammed Darwish Mohammed1, Wahid Hussein El-Dabae1, Khaled Abd El-Hamid Abd El‑Razik2, Amany Nabil Dapgh3, Ehab Ali Fouad4, Hussein Ahmed Abuelhag1

...infections therapy as carbapenems which is considered one of the human use last resorts. Extraintestinal Escherichia coli constitute the main etiologic factor implemented in urinary tract infections (UTIs) in humans and animals. The hypothesized risky role of companion animals in the load and dissemination of multidrug resistant uropathogenic E. coli (UPEC) which causes community-acquired UTI in pets and human community has been monitored. The current study ai...
Prevalence and Antibiotic Susceptibility of Thermophilic Campylobacter jejuni from Commercial Broiler Farms in and Around Lahore
Faiza Ghazanfar1, Masood Rabbani1*, Aamir Ghafoor2 and Muhammad Hassan Mushtaq3
...and ceftazidime (1%). Carbapenums (imipenum and meropenum) and cephalosporin (ceftazidime) was found to be highly effective against these MDR thermophilic C. jejuni isolates. Its efficacy for the treatment of campylobacteriosis should be further evaluated in clinical trials.

...
Effect of Different Strengths of MS Media and BAP on Banana Plantlet Growth in Vitro

Pangesti Nugrahani1*, Hery Purnobasuki2, Arif Nur Muhammad Ansori3, Jatuporn Anuchai4 and Anugerah Dany Priyanto5,6

Determination of Polycyclic Aromatic Hydrocarbon in Charcoal Beef Steak and Inhibitory Profile of Thyme Oil, Lactic Acid Bacteria and Marinating on their Existence

Ehab Hussein1, Edris A.M.1 and Ghada A.K. Kirrella2*

In vitro Culture of Dendrocalamus asper Bamboo in Liquid and Semi-Solid MS Media

Wan Nurfarzana Wan Mohamad Zani1, Norrizah Jaafar Sidik1*, Asmah Awal2, Nurul Izzati Osman3, Lyena Watty Zuraine Ahmad1 and Mohd Khairi Nordin4

Detection of blaNDM-1 gene among the carbapenem resistant Escherichia coli and Klebsiella pneumoniae isolates from a children’s hospital in Nepal

Binod G.C. 1 Nim Raj Sapkota 2 Binod Rayamajhee 2 Jayram Lamichhane 1 Pramod Poudel 1 Sunil Lekhak 3 Shovana Thapa 4 Santosh Khanal 1

Volume 2, Issue 5 September and October 2018 Pages 65-74
...aNDM-1 gene among the carbapenem resistant Escherichia coli and Klebsiella pneumoniae isolates, confirmed through using Modified Hodge Test (MHT). During the six months of this study (November, 2016 – April, 2017), a total of 1503 clinical samples including urine, blood, wound swabs and Cerebrospinal fluid (CSF) were collected and processed at Microbiology Laboratory, International Friendship Children’s Hospital, Kathmandu, Nepal. Among the E. coli...
Resistance prevalence profile of Klebsiella pneumoniae in the Intensive Care Units of Al- Shatby Pediatric Hospital, Alexandria, Egypt

 

Bassma H. Elwakil1; Safaa M. Ali2*; Soad F. Hafez3; Adnan A. Bekhit4; Moustafa Y. El-Naggar5; Zakia A. Olama5

Novel Research in Microbiology Journal (2019), 3(6): 535-545
... other hand, modified carbapenem inactivation and modified Hodge test (MHT) were used to assess the carbapenem resistant strains. It is observed that all the β-lactamase producers’ strains are also carbapenemase producers, whereas only nine strains (30%) are MHT positive. The Polymerase Chain Reaction (PCR) technique revealed that TEM, BETA, NDM and IPM genes are found on the ba...
Hypervirulent Klebsiella pneumoniae: Epidemiology, virulence factors, and antibiotic resistance

Enas M. Hefzy1*; Reda M. Taha2; Safaa Abd El Salam2; Abdelrhman Abdelmoktader1; Mahmoud A.F. Khalil3

...,
such as carbapenem-resistant hvKp isolates, is continuously growing. Multidrug resistance
(MDR) and increased virulence of these strains may be the cause of the subsequent clinical
crisis. This study aimed to review and analyse the epidemiology, the factors associated with
hypervirulence, and the mechanisms of antibiotic resistance of the hvKp strains in order to
provide a better understanding of th...
Colistin susceptibility among multidrug resistant Gram-negative bacilli isolated from Tertiary hospital in Egypt

 

Shimaa, A. Abdel Salam1; Raghda, Hager2*

Novel Research in Microbiology Journal (2020), 4(5): 968-978
... infections caused by carbapenem-resistant Gram-negative bacilli (GNB). Colistin resistance can increase due to the spread of plasmid-borne mcr-1 gene. This study aimed to determine colistin susceptibility and to detect the presence of mcr-1 gene in the clinical isolates of GNB recovered from different clinical samples collected from Ain Shams University Hospital, Cairo, Egypt. About thirty-five GNB isolates were recovered from the different clinical samples t...
Evaluating the performance of different detection methods of Carbapenemase producing Gram-negative bacilli isolated from surgical site infections

Rasha M. Elnagar

Novel Research in Microbiology Journal (2021), 5(2): 1194-1213
...hich are resistant to Carbapenem antibiotics. This study aimed to assess the performance of various detection methods of carbapenemase-producing GNB; isolated from healthcare associated SSIs at different surgical units, Mansoura University Hospitals, Al- Dakahliya Governorate, Egypt. A total of 186 wound specimens were collected from patients showing symptoms and signs of SSIs; used for isolation of bacteria and then identif...
Genotyping of carbapenem resistant Acinetobacter baumannii isolated from Egyptian patients

 

Heba Ahmed Mohamed1*; Gamal Fadl Mahmoud Gad1; Mona Fattouh Mohamed2; Hend Harby Ahmed3; Ameer Effat Elfarash4; Nahed Fathallah Fahmy2

Novel Research in Microbiology Journal (2022), 6(6): 1801-1820
...-acquired infections. Carbapenem resistant A. baumanni (CRAB) has been recorded to be resistant to nearly all antibiotics, including the last resort antibiotics; carbapenems. This study aimed to detect the carbapenem resistance levels and mechanisms, in addition to the genotyping of A. baumanni in Upper Egypt. About 200 clinical samples were collected from different wards of Sohag Universi...

Novel Research in Microbiology Journal

December

Issue 6

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