Search for Bacteriophages

Three Bacteriophages SA, SA2 and SNAF can Control Growth of Milk Isolated Staphylococcal Species

Aiza Tahir, Muhammad Asif, Zaigham Abbas and Shafiq ur Rehman*

...astitis. In ongoing era, bacteriophages are an alternate possible effective remedy. Therefore, in this study the infection ability of three lytic phages SA, SANF and SA2 was determined against ten isolates of Staphylococci and one Micrococcus, isolated from five raw milk samples. Their morphological, biochemical characterization and 16S rRNA sequencing indicated that two strains were S. aureus, while all others were different coagulase negative Staphylococci. ...

Storage of Bacteriophages at 4°C Leads to no Loss in Their Titer after One Year

Iqbal Ahmad Alvi, Muhammad Asif, Rabia Tabassum, Zaigham Abbas and Shafiq ur Rehman*

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Isolation of Pseudomonas aeruginosa Phages from Wastewaters

Stephen Chijioke Emencheta¹, Chinelo Charity Eze¹, Anthony Amaechi Attama², Damian Ejike Agbo¹ and Ebele Benedette Onuigbo¹*

...oth Pseudomonas spp. and bacteriophages were isolated from residential wastewaters. The isolated Pseudomonas spp. were confirmed through biochemical tests. Antibiogram was done with nine (9) different antibiotics, including;aztreonam (ATM), chloramphenicol (CPL), gentamicin (GTN), tetracycline (TTE), sulphamethoxazole/trimethoprim (SXT), amoxicillin/clavulanic acid (AMC), meropenem (MEM), ciprofloxacin (CIP), ceftriaxone (CRO)). Plaque assay was done to determ...

Isolation of Escherichia Coli Phages from Waste Waters

Stephen Chijioke Emencheta¹, Anthony Amaechi Attama², Ezinwanne Nneoma Ezeibe¹, Adaora Angela Agubata¹ and Ebele Benedette Onuigbo¹*

...oth Escherichia coli and bacteriophages were isolated from farmlands and residential wastewaters. The isolated Escherichia coli were confirmed through phenotypic and biochemical tests. Antimicrobial susceptibility test to nine antibiotics was determined. Multi-antibiotic resistance index (MARI) was assessed. Spot assay was done to determine lytic action of the bacteriophage on the lawns of the Escherichia coli. The phenotypic and biochemical tests confirmed Es...

Effect-of-Bacteriophages-on-Intestinal-Colonization-of-Escherichia-coli-Cecal-Microbiota-Composition-Intestinal-Morphology-and-Growth-Performance-in-Nursery-Pigs-from-Commercial-Pig-Farms

Pawinee Kingkan1, Thanathip Supcharoenkul1, Chaowit Rakangthong1, Chaiyapoom Bunchasak1, Komwit Surachat2,3, Wiriya Loongyai1*

Cocktail Phage Therapy for Bacteria Contaminating Meat in Egypt

Amany M. Reyad¹*, Aya Maher Rabie¹, Reda Mohamed Taha¹ and Khalid El-Dougdoug²

Physiological Profiling of Lytic Bacteriophages and their Efficiency against Salmonella Enteritidis on Chicken Breast Cuts

Aleena Kokab¹, Ali Ahmad Sheikh¹*, Masood Rabbani¹, Wasim Shehzad², Muhammad Ilyas Riaz¹, Sohail Raza¹ and Rida Haroon Durrani¹

...-food sector. The use of bacteriophages, as a potential replacement, for antibiotics and as food antimicrobial agents to potentially target foodborne pathogens is gaining augmented attention. This study’s goal was to isolate and physiologically characterize lytic bacteriophages against Enteritidis, and to ascertain their efficacy in modulating bacterial population on chicken breast cuts in the form of a phage cocktail....

Isolation and Characterization of a Pseudomonas aeruginosa Bacteriophage from Agriculture Drain Water

Amina A. II. and M.K. Amin

...xt-align: justify;">Five bacteriophages infecting Pseudomonas aeruginosa strain were isolated from agricultural drain water of Zagazig city. One of them designated as PAI has been characterized. The ability of phage PAI to form plaques with strain PAOI was examined. Two kinds of plaques (turbid and clear) have been observed. The average number of turbid plaques was 2.52 x 109 pfu/ml whereas of clear plaques was 9.96 x 107 pfu/n) l. The phage was able to lysis ...

Isolation and Characterization of a Pseudomonas Putida Bacteriophages

Mayada A. Abd Elgalell; B.A Othman2; Th. Radwan, 1 and Amal S.M. Abo-Sinna3

Evolution of super-drug resistant microbial strains: mechanisms and strategies for containment

SHEIKH AJAZ RASOOL1, MUHAMMAD SALMAN RASOOL2 & MUNAZZA AJAZ3

...as, probiotics, CRISPRs, bacteriophages, nanotechnology and phytochemicals.

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Using Bacteriophage in Poultry Diet as Therapeutics Against Escherichia coli in Broiler Chickens

Dheyaa Ali Aljuhaishi*, Firas Hussain Kadim Albawi

Application of bacteriophages isolated from sewage to control urinary O157:H7 Escherichia coli and several bacterial uropathogens

Salwa M. Masoud1; Hend A. Refat2; Nada S. Sayed2; Mohamed K. Abd-El Aal2; Ahmed A. Dosocky2; Zeinab S. Mohammed3; Mohamed A. H. Abdel Wahab3; Dina M. Mekawy3; Ali I. Ali4; Basma A. Atya4; Katren T. Welliam4; Rehab M. Abd El-Baky1, 5; Zeinab S. Hashem1*

Novel Research in Microbiology Journal (2020), 4(5): 1005-1014

...he effect of waste water bacteriophages on the growth of some multi-drug resistance Escherichia coli and other bacterial uropathogens. Urine samples were collected from 30 UTI patients at Minia University Hospital, Minia, Egypt. Twenty Escherichia coli strains were isolated from UTI patients. The Kirby-Bauer disc diffusion method was used to determine the isolates antibiotic susceptibility. The isolates showed resistance to ciprofloxacin and levofloxacin by 70...

Bacteriophages as affordable solution for treatment of multidrug resistant bacteria, and their recent potential applications

Ghadah A. Alsubhi1,2*

Novel Research in Microbiology Journal (2021), 5(6): 1405-1414

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The impact of bacteriophages as antibacterial agents was known after their first discovery in 1915, and they were then employed to treat the bacterial infections. However, the discovery of penicillin in 1928 quickly overshadowed bacteriophage therapy, and paved the way for its large-scale production in the 1940s to enter the era of antibiotics. In recent years, resistant bacteria have contributed to increasing the studies on

Exploiting biological tools for post-antibiotic era: novel sustainable strategies against antimicrobial resistance

Omnia Karem Riad*

Novel Research in Microbiology Journal (2024), 8(6): 2734-2749

... predatory bacteria, and bacteriophages, which are powerful tools valuable to fighting resistant microorganisms with fewer chances of resistance development. Naturally synthesized products such as antimicrobial peptides and bacteriocins revealed successful treatment options. Additionally, the Clustered regularly interspaced short palindromic repeats (CRISPR-Cas) system is a gene-editing tool that can re-sensitize the resistant bacteria. Vaccination prevents in...