Muhammad Usman Qamar1, 2, Sidrah Saleem1, Usman Arshad1, Muhammad Farhan Rasheed3, Hasan Ejaz4, Naveed Shahzad5 and Shah Jahan6,*
...akistan. A total of five carbapenem resistant bacterial species; E. coli, K. pneumoniae, E. cloacae, A. baumannii and P. aeruginosa, were isolated and identified using VITEK 2 compact system. Carbapenamase and metallo-β-lactamase (MBL) production by these isolates was confirmed by modified Hodge’s test and disc potentiation method, respectively. The blaNDM-1genewas amplified by PCR and sequenced. MIC of different anti...
Sahar Naz1, Farhan Rasheed2, Muhammad Saeed3*, Shagufta Iram4 and Ambereen Anwar Imran5
...idelines. Next day every carbapenem-resistant isolate were further processed for MβL detection by EDTA method, zone size of Carbapenem disc only and Carbapenem disc impregnated with EDTA was compared ( >7 mm increase MβL positive, 0-5 mm increase MβL-negative).
Results: Out of total 12126 samples, 35.9% (n=4361) were culture positive and only 40.5% (n=1...
Junaid Akhtar1,2, Sidrah Saleem1, Naveed Shahzad3, Abdul Waheed1, Iqra Jameel1, Farhan Rasheed4 and Shah Jahan5,*
...ntibiotics predominantly carbapenems, are considered as last treatment option against multi-drug resistance GNR infections. However, the emergence of carbapenemases particularly metallo β-lactamases (MBLs) in bacteria have severely mitigated the efficiency of carbapenems. MBLs producing gram-negative bacteria have been reported from various hospital settings, worldwide. However, data ...
Muhammad Hidayat Rasool1,*, Muhammad Zaheer1, Muhammad Farooque Hassan2, Muhammad Shafique1 and Muhammad Usman Qamar1
...ration cephalosporin and carbapenems is an issue of high importance. Present study was aimed to determine the frequency and antimicrobial susceptibility patterns of MBL producing carbapenem-resistant Gram-negative rods (GNRs) isolated from clinical specimens. A clinical descriptive study was conducted in Faisalabad, Pakistan from June 2016 to January 2017. A total of 152 clinical samples were processed according to standard ...
Shafqat Husnain Khan1, Shah Jahan2, Irshad Ahmad3,4, Sadeeq ur Rahman5,* and Tayyab ur Rehman4,*
...y, increasingly reported carbapenem resistance due to carbapenemases is highly worrisome. Literature regarding incidence of class B metallo-β-lactamases (MBLs) - producing E. coli has not yet judiciously been addressed. In this study, we investigated the occurrence of carbapenemase- and MBLs-producers among a collection of 100 carbapenem resi...
Farzana Rashid1, Rabia Masood1 and Mariam Faiz2*
...oncern. A careful use of carbapenems is important to prevent the spread of these organisms.
...
Sana Ilyas1, Muhammad Hidayat Rasool1,*, Muhammad Javed Arshed2, Muhammad Usman Qamar1 and Bilal Aslam1
...the presence of ESBL and carbapenemase-producing genes using PCR. Of 392 samples, 219 ESBL positive E. coli were recovered and among these 156/213 (73.2%), 42/63 (66.6%) and 21/27 (77.7%) were from poultry, environmental water, and urine samples, respectively. The PCR results revealed that71.2% of blaCTX-M, 67.5% blaCTX-M -1 and 62.2% blaTEM producing isolates were recovered from poultry, 19.1% bl...
Mona Kadry, Sara Mohamed Nader*, Esraa A. Elshafiee and Zeinab S. Ahmed
...ental spread of ESBL and carbapenemase - producing Salmonella spp, which could pose a potential hazard to human and animal health. A total of 334 chicken meat samples, 197 eggshells and 160 human stool specimens were included in this study. The presence of salmonellae spp. was examined using bacteriological isolation and serological identification. PCR amplification of the ESBL and carbapenemase-encoding genes was performed....
Aqsa Shahid1, Saima Muzammil1, Farhan Rasheed2,Bilal Aslam1, Muhammad Akhtar Ali3, Syed Zohaib Haider4, Masroor Ellahi Babar4, Muhammad Saeed1, Umair Waqas1 and Mohsin Khurshid1,*
...tance to cephalosporins, carbapenems, aminoglycosides, and fluoroquinolones whereas colistin and tigecycline were the most active antimicrobial agents against A. baumannii as none of the isolates was found resistant to these two drugs. Moreover, the armA was found in 25% (n=37) of A. baumannii isolates which showed a high level of resistance to aminoglycosides i.e., amikacin; ≥256µg/ml (Breakpoints; ≥64µg/ml) and gentamic...
Farheen Aslam*, Hira Lodhi, Rasheeda Bashir, Faiza Saleem and Shagufta Naz
...alence of blaNDM-1 among carbapenem resistant enterobacteriaceae isolated from patient suffering from different diseases at local tertiary care hospital of Lahore....
SHEIKH AJAZ RASOOL1, MUHAMMAD SALMAN RASOOL2 & MUNAZZA AJAZ3
Ashraf Samir Hakim1*, Doaa Diab Khalaf1, Engy Farahat1, Mohammed Darwish Mohammed1, Wahid Hussein El-Dabae1, Khaled Abd El-Hamid Abd El‑Razik2, Amany Nabil Dapgh3, Ehab Ali Fouad4, Hussein Ahmed Abuelhag1
...an infections therapy as carbapenems which is considered one of the human use last resorts. Extraintestinal Escherichia coli constitute the main etiologic factor implemented in urinary tract infections (UTIs) in humans and animals. The hypothesized risky role of companion animals in the load and dissemination of multidrug resistant uropathogenic E. coli (UPEC) which causes community-acquired UTI in pets and human community has been monitored. The current study...
Binod G.C. 1 Nim Raj Sapkota 2 Binod Rayamajhee 2 Jayram Lamichhane 1 Pramod Poudel 1 Sunil Lekhak 3 Shovana Thapa 4 Santosh Khanal 1
Volume 2, Issue 5 September and October 2018 Pages 65-74
... blaNDM-1 gene among the carbapenem resistant Escherichia coli and Klebsiella pneumoniae isolates, confirmed through using Modified Hodge Test (MHT). During the six months of this study (November, 2016 – April, 2017), a total of 1503 clinical samples including urine, blood, wound swabs and Cerebrospinal fluid (CSF) were collected and processed at Microbiology Laboratory, International Friendship Children’s Hospital, Kathmandu, Nepal. Among the E. c...
Bassma H. Elwakil1; Safaa M. Ali2*; Soad F. Hafez3; Adnan A. Bekhit4; Moustafa Y. El-Naggar5; Zakia A. Olama5
Novel Research in Microbiology Journal (2019), 3(6): 535-545
...the other hand, modified carbapenem inactivation and modified Hodge test (MHT) were used to assess the carbapenem resistant strains. It is observed that all the β-lactamase producers’ strains are also carbapenemase producers, whereas only nine strains (30%) are MHT positive. The Polymerase Chain Reaction (PCR) technique revealed that TEM, BETA, NDM and IPM genes are found on the...
