Junaid Akhtar1,2, Sidrah Saleem1, Naveed Shahzad3, Abdul Waheed1, Iqra Jameel1, Farhan Rasheed4 and Shah Jahan5,*
...owever, the emergence of carbapenemases particularly metallo β-lactamases (MBLs) in bacteria have severely mitigated the efficiency of carbapenems. MBLs producing gram-negative bacteria have been reported from various hospital settings, worldwide. However, data is lacking in Pakistan regarding their frequency particularly among GNRs. Therefore, the present study aimed at determining the frequency of carbapenemase and &b...
Shafqat Husnain Khan1, Shah Jahan2, Irshad Ahmad3,4, Sadeeq ur Rahman5,* and Tayyab ur Rehman4,*
...apenem resistance due to carbapenemases is highly worrisome. Literature regarding incidence of class B metallo-β-lactamases (MBLs) - producing E. coli has not yet judiciously been addressed. In this study, we investigated the occurrence of carbapenemase- and MBLs-producers among a collection of 100 carbapenem resistant E. coli obtained from a tertiary hospital at Lahore Pakistan. All ...
Sana Ilyas1, Muhammad Hidayat Rasool1,*, Muhammad Javed Arshed2, Muhammad Usman Qamar1 and Bilal Aslam1
...the presence of ESBL and carbapenemase-producing genes using PCR. Of 392 samples, 219 ESBL positive E. coli were recovered and among these 156/213 (73.2%), 42/63 (66.6%) and 21/27 (77.7%) were from poultry, environmental water, and urine samples, respectively. The PCR results revealed that71.2% of blaCTX-M, 67.5% blaCTX-M -1 and 62.2% blaTEM producing isolates were recovered from poultry, 19.1% bl...
Mona Kadry, Sara Mohamed Nader*, Esraa A. Elshafiee and Zeinab S. Ahmed
...ental spread of ESBL and carbapenemase - producing Salmonella spp, which could pose a potential hazard to human and animal health. A total of 334 chicken meat samples, 197 eggshells and 160 human stool specimens were included in this study. The presence of salmonellae spp. was examined using bacteriological isolation and serological identification. PCR amplification of the ESBL and carbapenemase-encoding genes was performed....
SHEIKH AJAZ RASOOL1, MUHAMMAD SALMAN RASOOL2 & MUNAZZA AJAZ3
Binod G.C. 1 Nim Raj Sapkota 2 Binod Rayamajhee 2 Jayram Lamichhane 1 Pramod Poudel 1 Sunil Lekhak 3 Shovana Thapa 4 Santosh Khanal 1
Volume 2, Issue 5 September and October 2018 Pages 65-74
...ere screened as possible carbapenemase producers. For screening of carbapenemase enzyme production among these isolates, resistances to third generation cephalosporins and carbapenems were taken into consideration. Two isolates of E. coli and 3 isolates of K. pneumoniae were confirmed as carbapenemase producers by MHT. Furthermore, Polymerase chain reaction (PCR) was carried out among the ...
Bassma H. Elwakil1; Safaa M. Ali2*; Soad F. Hafez3; Adnan A. Bekhit4; Moustafa Y. El-Naggar5; Zakia A. Olama5
Novel Research in Microbiology Journal (2019), 3(6): 535-545
...’ strains are also carbapenemase producers, whereas only nine strains (30%) are MHT positive. The Polymerase Chain Reaction (PCR) technique revealed that TEM, BETA, NDM and IPM genes are found on the bacterial plasmid (100%). However the presence of the β-lactamase genes on the bacterial DNA varied among the different strains. The presence of the resistance genes on the bacterial plasmid may signify the resistance acquired upon the previous exposure...
Rasha M. Elnagar
Novel Research in Microbiology Journal (2021), 5(2): 1194-1213
...ous detection methods of carbapenemase-producing GNB; isolated from healthcare associated SSIs at different surgical units, Mansoura University Hospitals, Al- Dakahliya Governorate, Egypt. A total of 186 wound specimens were collected from patients showing symptoms and signs of SSIs; used for isolation of bacteria and then identification of these bacterial isolates according to colony morphology; microscopic examination and biochemical reactions. About 173 spe...
Heba Ahmed Mohamed1*; Gamal Fadl Mahmoud Gad1; Mona Fattouh Mohamed2; Hend Harby Ahmed3; Ameer Effat Elfarash4; Nahed Fathallah Fahmy2
Novel Research in Microbiology Journal (2022), 6(6): 1801-1820
...CR testing of the common carbapenemase-encoding genes, including OXA-51, OXA-58, KPC, GES, IMP, NDM, VIM, SIM, and GIM. Genotyping was performed using the Enterobacterial Repetitive Intergenic Consensus-Polymerase chain reaction (ERIC-PCR). About 85 % of A. baumannii strains were multidrug resistant (MDR), and high rate of Extreme drug resistant (XDR) A. baumannii (70 %) was detected. Carbapenem resistance was detected in 65 % of A. baumannii isolates, 70.58 %...
