Dan Yü, Chuchu Li, Yü Huang and Zhen Huang*
... mycoparasitism. The cell-free culture supernatant and ethylacetate extracts from T. hamatum inhibited mycelial growth of S. sclerotiorum, with EC50 value of 0.54 µl/cm2 and 2.99 µg/ml, respectively. The sclerotia production of S. sclerotiorum decreased when treated with ethylacetate extracts. The level of additive inhibition on S. sclerotiorum using both fungal ethylacetate extracts and difenoc...
Erum Yawar Iqbal1*, Ashfaque Ahmed Nahiyoon1, Shahnaz Dawar2 and Shahina Fayyaz1
...cterial culture such as, cell-free supernatant, crude cell extract, bacterial culture and methanol extract in in vitro and green house condition have been exploited. The results have shown that different formulants of EPB produced significant effects on Aphid mortality, change in population density, population reduction and fecundity. Cell-free supernatant of new EPB species Xenorhabdus steinernematis n.sp. ...
Wenyou Huang1, Dan Yü1, Song Huang1,2, Jian Xiao2, Ping Qi2, Anhua Song2* and Zhen Huang1*
...ntact insect toxicity of cell-free culture supernatants and the EthOAc extracts derived from the MlUV-40b mutant were 1.2-3 times more potent than that of the wild type. A simple approach coupling mutagenesis and growth & second metabolites were used to isolate strains with increased stress resistance and virulence. Increased virulence in some of the mutants correlated with increased insecticidal activity in cell-free ex...
A.W. Amin†, A.A. Anter, A.H. Ashoub* and A.S. El-Nuby*
...e population followed by cell-free culture filtrates, bacterial live cells and
bacterial dead cells, sequentially. It was concluded that bacteria has induced tomato resistance or bio-control effects
against M. incognita in tomato.
... 
Mostafa R. Zaher1,2*, Amir A. Shehata1, Azza M. El Amir2, Naglaa M. Hagag1, Reham H. Tammam3**
... exosome-based vaccines, cell-free protein synthesis systems, self-amplifying RNA vaccines, and the application of artificial intelligence (AI) and computational biology in vaccine design. These methodologies may offer solutions for the challenges posed by the foot-and-mouth disease virus’s high mutation rate, antigenic variability, and the need for enhanced protection and thermostability.
Keywords | Foot-and-mouth disease,...
Humaira1, Afsana Huseynova Anvar2, Shaukat Ali3, Marcelo Franco4, Muhammad Irfan1*
...ddress these challenges, cell-free approaches developed as promising platform to proceed towards sustainable bio-manufacturing through the development of self-sustaining, constantly operating systems employing renewable biomass to yield higher concentrations and productivity of extended-range of products. To-date established cell-free systems including extract-based and purified enzyme-based cell-fre...
Abd-Elhalim, B.T.1*; Gamal, R.F.1; Abou-Taleb, Kh.A.1; Haroun, A.A.2
Novel Research in Microbiology Journal (2019), 3(6): 558-578
... P. silesinsis strain A3 cell-free supernatant was carried out using seven agro-industrial residues, added to the basal medium as different carbon sources. Results showed that 2% blackstrap sugar cane molasses was the most efficient carbon source for CuNPs biosynthesis, when incubated for at 30°C for 24 h using shaking speed of 120 rpm. The biosynthesized CuNPs has a size of 66.12 nm at a concentration of 19.2 mg\ l, and maximum surface plasmon peak (SPR) ...
Amany A. Hassabo1; Basant A. Ali1; Shaimaa A. Tawfik2; Hossam E. Emam3*
Novel Research in Microbiology Journal (2024), 8(5): 2666-2682
...esis of Cu-Se BNPs using cell-free extract of a marine bacterium, and studying their anticancer and antioxidant activities. Based on 16S rDNA sequencing, the isolated marine bacterium was identified Bacillus amyloliquefaciens by phylogenetic analysis. Biogenic synthesis of Cu-Se BNPs was optimized by studying the effect of some physiological factors such as reaction time, pH, and temperature. Based on the reaction conditions, the biologically synthesized Cu-Se...
