Search for Cellulase

Effect of alkali treatment (NaOH) of eucalyptus leaves for hypercellulase production by Bacillus subtilis through submerge fermentation

Sidra Iqbal1, Muhammad Irfan2*, Hafiz Abdullah Shakir1, Fouzia Tabbsum1, Javed Iqbal Qazi1

...si). After pretreatment, cellulase production was conducted in 250ml Erlenmeyer flask at 50 ºC, pH 5 with shaking speed of 120 rpm using 2% pretreated eucalyptus leaves as carbon source and 2% v/v inoculum size in submerged fermentation for 24h. Results showed that among these two treatments, only chemical treated substrates gave better enzyme yield as compared to chemical followed by steam. The highest FPase (2.526 IU/ml/min) activity was found at pretre...

Optimization of dilute sulphuric acid pretreatment of peanut shells through Box- Bhenken design for cellulase production by Bacillus subtilis K-18

Fatima Arshad1, Muhammad Irfan2*, Hafiz Abdullah Shakir3, Fouzia Tabbsum3, Javed Iqbal Qazi3

...found more effective for cellulase production as compared to thermochemical pretreatment. The maximum CMCase activity was 1.757 1U/ml/min under conditions of 0.6% acid concentration, 10% substrate concentration, and resident time of 4h. The maximum FPase activity was 2.015 1U/ml/min under conditions of 0.8% acid concentration, 10% substrate concentration, and time of 6h. This showed that peanut shells could successfully be used as substrate for

Dilute Sulphuric Acid Pretreatment Optimization of Cotton Stalk for Cellulase Production through Box-Bhenken Design

Noor Fatima¹, Muhammad Irfan^2,*, Hafiz Abdullah Shakir¹, Javed Iqbal Qazi¹

...act | In this study, cellulase enzyme production was assessed by pretreating cotton stalk with dilute sulphuric acid which was optimized through response surface methodology. Pretreatment conditions were optimized using three variable with three levels like sulfuric acid concentrations (0.6%, 0.8%, 1% v/v), biomass loading (5%, 10%, 15% w/v), and residence time (4, 6 and 8h). After pretreatment process, cellulase product...

Enhanced Activities of Metabolic Enzymes Associated with Insecticide Resistance in Red Flour Beetle (Tribolium castaneum Herbst)

Muhammad Asam Riaz¹, Javeria Choudhary¹, Muhammad Zeeshan Majeed^1*, Muhammad Afzal¹, Sohail Ahmed² and Muhammad Sajjad Khalil¹

...tly higher activities of cellulases. Enzyme kinetics results demonstrated that Swl-strain had a maximum value of Vmax (68.4 Nm s-1) and lowest value of Km (12.8 g L-1). Fsd- and Swl-strains exhibited increased activities of amylases with Vmax value of 2.68 and 1.89 µM s-1 respectively and Km value of 23.8 g L-1 and 40.1 g L-1, respectively. These findings suggest that the increased activities of cellulases and amylases...

Optimization of medium and substrate for CMCase production by Bacillus subtilis-BS06 in submerged fermentation and its applications in saccharification

Mohsin Arshad1, Muhammad Irfan*2, Asad-Ur-Rehman1, Muhammad Nadeem3, Zile Huma3, Hafiz Abdullah Shakir4, Quratulain Syed3

Optimization of medium and substrate for CMCase production by Bacillus subtilis-BS06 in submerged fermentation and its applications in saccharification

...trates for carboxymethyl cellulase
(CMCase) production under static and with agitation speed of 140 rpm. The whole
fermentation experiments were carried out in 250ml Erlenmeyer flask. Different
agricultural wastes such as sugarcane bagasse, wheat straw, rice husk, defatted
soybean meal, corn cobs and wheat bran were exploited for CMCase production
using different media (medium 1 (M1), medium II (M-II), medium III (M-III) &...

Cloning, Expression and Characterization of Highly Active Recombinant Thermostable Cellulase from Thermotoga naphthophila

Aisha Khalid¹, Muhammad Tayyab^1,*, Abdual Rauf Shakoori², Abu Saeed Hashmi¹, Tahir Yaqub³, Ali Raza Awan¹, Muhammad Wasim¹, Sehrish Firyal¹, Zaheer Hussain⁴ and Munir Ahmad⁵

...recombinant thermostable cellulase from Thermotoga naphthophila. PCR using the genomic DNA of T. naphthophila as template resulted in amplification of 1 kb cellulase gene. The amplified cellulase gene was cloned in pTZ57R/T and sub-cloned in pET28a. The expression of recombinant cellulase was analyzed using BL21 CodonPlus (DE3) cells as exp...

Optimization of Conditions for Maximal Production of Recombinant Thermostable Cellulase from Thermotoga naphthophila using E. coli BL21-CodonPlus (DE3) as Expression Host

Aisha Khalid¹, Muhammad Tayyab^1,*, Abu Saeed Hashmi¹, Tahir Yaqub², Ali Raza Awan¹, Muhammad Wasim¹, Shagufta Saeed¹, Sehrish Firyal¹and Abdul Rauf Shakoori³

...oduction of thermostable cellulase. Production of cellulase was focused due to its diverse range of application in industry. In the present study, conditions were optimized for the maximal production of recombinant thermostable cellulase from Thermotoga naphthophila using BL21-CodonPlus (DE3) cells as expression host and pET28a as expression vector. Effect of various concentration o...

Separation of Enzymes from their Aqueous System by using Novel Concept of Unidirectional Freezing

Muhammad Waheed Mushtaq^1,*, Muhammad Waqar Younas², Jamil Anwar², Syed Mustansar Abbas³and Shahid Bashir³

... enzymes (alpha-amylase, cellulase and pectinase) from aqueous system has been studied by applying novel concept of unidirectional freezing. To separate the enzymes from their aqueous system, directional freezing was applied horizontally through synthetic enzyme contaminated water samples, called radial freezing. After complete freezing, the samples were collected from various sections of the frozen mass and concentration of enzymes was determined. It was foun...

Utilization of Bombyx ceiba Seed Pods: A Novel Substrate for Cellulase Production through Solid State Fermentation using Response Surface Methodology

Sobia Nazir¹, Muhammad Irfan¹*, Muhammad Nadeem², Hafiz Abdullah Shakir³*, Muhammad Khan³, Shaukat Ali⁴, Quratulain Syed²

... viride was used for cellulase production in solid state fermentation (SSF) using seed pods of Bombyx ceiba as substrate. Three parameters were used such as inoculum size (1, 5.5 and 10%), medium volume (1, 3 and 5ml) and incubation period (3, 4, 5 days) to observed the maximum activity of cellulase using Box-Bhenken design of response surface methodology. Maximum activity of CMCase (0.700 IU/gds/min) and FPase (0...

Cellulase Production by Trichoderma viride in Submerged Fermentation using Response Surface Methodology

Tehreem Zahra¹, Muhammad Irfan¹, Muhammad Nadeem², Misbah Ghazanfar¹, Qurratulain Ahmad³, Shaukat Ali⁴, Farzana Siddique⁵, Zarina Yasmeen⁶, Marcelo Franco⁷

...ichoderma viride for cellulase production using seed pods of silk cotton tree as substrate in submerged fermentation by response surface methodology has been investigated. Three variables like substrate concentration, peptone and KH₂PO₄were optimized at three levels. The optimum carboxymethylcellulase (CMCase) activity and filter paper cellulase (FPase) activity ...

Influence of Exogenous AHLs and Quorum Quenching AiiA Protein on the Production of Cellulase Enzyme in Marine Snow Associated Bacterium, Citrobacter freundii B1

Abdul Nabi Jatt^1,2*

...duction of extracellular cellulase enzyme. Mainly, two different reporter strains such as Agrobacterium tumefaciens A136 and Chromobacterium violaceum CV026 were applied to confirm the production of signal molecules. The identification of signal molecules was achieved by GC-M...

Evolutionary Adaptation of Fermenting Strains in Wheat Straw Hydrolysate for Bioethanol and Lactic Acid Production

Imrana Khushk¹, Abdul Nabi Jatt², Abdul Sattar Qureshi¹*, Choudhary Haider Ali³, Muhammad Aqeel Bhutto¹, Imran Ibrahim Khaskheli¹, Sartar Iqbal Panhwer¹

...t inhibitors and cost of cellulase enzyme). In the present study, long term evolutionary adaptation was evaluated for ethanol and lactic acid production to overcome the pretreatment inhibitors issue. Saccharomyces cerevisiae Angel was used for the production of ethanol, while, Bacillus coagulans, Pediococcus sp. and Lactobacillus sp. were used for lactic production, respectively. All the microbial strains were inoculated on wheat straw hydrolysate and were mon...

Improving Azolla microphylla through Fermentation with Lignocellulolytic Fungi and its Application in Broiler Feed

Nuraini Nuraini*, Mirzah Mirzah, Yuliaty Shafan Nur, Harnentis Harnentis

... The parameter measured: cellulase activity, crude fiber, fiber digestibility, crude protein, and nitrogen retention. The results of phase 1 showed that the types of fungi had a very significant effect (P <0.01) on the nutrient quality of fermented Azolla microphylla. Fermentation of Azolla microphylla with Lentinus edodes and Pleurotus ostreatus gave the best products. Phase 2. Application Fermented Azolla microphylla (AMF) in the diet on broiler performa...

Screening, Characterization and Purification of Cellulase from Mango Mealybug Drosicha stebbingi Green (Homoptera: Margarodidae)

Mubasshir Sohail*, Qadeer Ahmed Soomro, Raza Muhammad, Muhammad Usman Asif and Imran Rauf

Fermentation Characteristics, In Vitro Nutrient Digestibility, and Methane Production of Oil Palm Frond-Based Complete Feed Silage Treated with Cellulase

Budi Santoso*, Bambang Tjahyono Hariadi, Marlyn Nelce Lekitoo

...o assess the impact of a cellulase additive. In the complete randomized design experiment, six treatments (silage) were used: A, B, C, D, E, and F. Treatment A consisted of oil palm frond (40%), king grass (30%), cassava (10%), tofu waste (10%), molasses (7%), lactic acid bacteria (LAB) inoculant (3%), and cellulase 0 ml/kg. Treatment B comprised silage A + 3 ml cellulase/kg, while C used ...

Optimization of Cellulase Production by Chaetomium thermophilum in Submerged Fermentation using Wheat Straw as Substrate

Sabahat Anwar¹, Faria Javaid² and Qurat-ul-Ain²*

...d industrially important cellulase enzymes by using a thermophilic fungus i.e., Chaetomium thermophilum, which can efficiently produce cellulases extracellularly while growing on cellulosic substrates. Wheat straw, by product of wheat crop has been used as lignocellulosic substrate. To determine the optimum conditions for maximum production, experiment was carried out at various substrate concentrations, temperatures and pH ...

Enhancing Broiler Chick Growth with Locally Produced Thermostable Cellulase in Fibrous Diets

Aisha Khalid1,2, Roheela Yasmeen1*, Farah Ahmad1, Mudassar Aslam2, Muhammad Tayyab2

...recombinant thermostable cellulase from Thermotoga naphthophila (CELTN) on the growth performance of broiler chicks when added to corn-based feed. The thermostable cellulase was produced using BL21 CodonPlus cells with the pET28a-Cellu vector under optimized conditions. A total of 150 one-day-old, unsexed broiler chicks were divided into five treatment groups, each further subdivided into three replicates of 10 chicks with a...

Effects of Storage Time on the Chemical and Microbiological Quality of Pasteurized Beetroot (Beta vulgaris L.) Juice with Enzyme Extraction and Clarification

Abdussalam Muhyideen Zainab Funmilayo and Lee-Hoon Ho*

...ified with pectinase and cellulase and their combination, at concentration of 1.0 U/ mL for 50°C and 15 min. After that, the mixtures were all kept at 90°C for five minutes in order to pasteurize the juice. Beetroot juice without treatment: fresh juice and juice with enzyme extraction but not pasteurised nor clarified served as control 1 and control 2, respectively. The juices were evaluated for pH, total titratable acidity, total soluble solids, vitam...