Sidra Iqbal1, Muhammad Irfan2*, Hafiz Abdullah Shakir1, Fouzia Tabbsum1, Javed Iqbal Qazi1
...si). After pretreatment, cellulase production was conducted in 250ml Erlenmeyer flask at 50 ºC, pH 5 with shaking speed of 120 rpm using 2% pretreated eucalyptus leaves as carbon source and 2% v/v inoculum size in submerged fermentation for 24h. Results showed that among these two treatments, only chemical treated substrates gave better enzyme yield as compared to chemical followed by steam. The highest FPase (2.526 IU/ml/min) activity was found at pretre...
Fatima Arshad1, Muhammad Irfan2*, Hafiz Abdullah Shakir3, Fouzia Tabbsum3, Javed Iqbal Qazi3
...found more effective for cellulase production as compared to thermochemical pretreatment. The maximum CMCase activity was 1.757 1U/ml/min under conditions of 0.6% acid concentration, 10% substrate concentration, and resident time of 4h. The maximum FPase activity was 2.015 1U/ml/min under conditions of 0.8% acid concentration, 10% substrate concentration, and time of 6h. This showed that peanut shells could successfully be used as substrate for
Noor Fatima1, Muhammad Irfan2,*, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1
...act | In this study, cellulase enzyme production was assessed by pretreating cotton stalk with dilute sulphuric acid which was optimized through response surface methodology. Pretreatment conditions were optimized using three variable with three levels like sulfuric acid concentrations (0.6%, 0.8%, 1% v/v), biomass loading (5%, 10%, 15% w/v), and residence time (4, 6 and 8h). After pretreatment process, cellulase product...
Muhammad Asam Riaz1, Javeria Choudhary1, Muhammad Zeeshan Majeed1*, Muhammad Afzal1, Sohail Ahmed2 and Muhammad Sajjad Khalil1
...tly higher activities of cellulases. Enzyme kinetics results demonstrated that Swl-strain had a maximum value of Vmax (68.4 Nm s-1) and lowest value of Km (12.8 g L-1). Fsd- and Swl-strains exhibited increased activities of amylases with Vmax value of 2.68 and 1.89 µM s-1 respectively and Km value of 23.8 g L-1 and 40.1 g L-1, respectively. These findings suggest that the increased activities of cellulases and amylases...
Mohsin Arshad1, Muhammad Irfan*2, Asad-Ur-Rehman1, Muhammad Nadeem3, Zile Huma3, Hafiz Abdullah Shakir4, Quratulain Syed3
Optimization of medium and substrate for CMCase production by Bacillus subtilis-BS06 in submerged fermentation and its applications in saccharification
...trates for carboxymethyl cellulase
(CMCase) production under static and with agitation speed of 140 rpm. The whole
fermentation experiments were carried out in 250ml Erlenmeyer flask. Different
agricultural wastes such as sugarcane bagasse, wheat straw, rice husk, defatted
soybean meal, corn cobs and wheat bran were exploited for CMCase production
using different media (medium 1 (M1), medium II (M-II), medium III (M-III) &...
(CMCase) production under static and with agitation speed of 140 rpm. The whole
fermentation experiments were carried out in 250ml Erlenmeyer flask. Different
agricultural wastes such as sugarcane bagasse, wheat straw, rice husk, defatted
soybean meal, corn cobs and wheat bran were exploited for CMCase production
using different media (medium 1 (M1), medium II (M-II), medium III (M-III) &...
Aisha Khalid1, Muhammad Tayyab1,*, Abdual Rauf Shakoori2, Abu Saeed Hashmi1, Tahir Yaqub3, Ali Raza Awan1, Muhammad Wasim1, Sehrish Firyal1, Zaheer Hussain4 and Munir Ahmad5
...recombinant thermostable cellulase from Thermotoga naphthophila. PCR using the genomic DNA of T. naphthophila as template resulted in amplification of 1 kb cellulase gene. The amplified cellulase gene was cloned in pTZ57R/T and sub-cloned in pET28a. The expression of recombinant cellulase was analyzed using BL21 CodonPlus (DE3) cells as exp...
Aisha Khalid1, Muhammad Tayyab1,*, Abu Saeed Hashmi1, Tahir Yaqub2, Ali Raza Awan1, Muhammad Wasim1, Shagufta Saeed1, Sehrish Firyal1 and Abdul Rauf Shakoori3
...oduction of thermostable cellulase. Production of cellulase was focused due to its diverse range of application in industry. In the present study, conditions were optimized for the maximal production of recombinant thermostable cellulase from Thermotoga naphthophila using BL21-CodonPlus (DE3) cells as expression host and pET28a as expression vector. Effect of various concentration o...
Muhammad Waheed Mushtaq1,*, Muhammad Waqar Younas2, Jamil Anwar2, Syed Mustansar Abbas3 and Shahid Bashir3
... enzymes (alpha-amylase, cellulase and pectinase) from aqueous system has been studied by applying novel concept of unidirectional freezing. To separate the enzymes from their aqueous system, directional freezing was applied horizontally through synthetic enzyme contaminated water samples, called radial freezing. After complete freezing, the samples were collected from various sections of the frozen mass and concentration of enzymes was determined. It was foun...
Sobia Nazir1, Muhammad Irfan1*, Muhammad Nadeem2, Hafiz Abdullah Shakir3*, Muhammad Khan3, Shaukat Ali4, Quratulain Syed2
... viride was used for cellulase production in solid state fermentation (SSF) using seed pods of Bombyx ceiba as substrate. Three parameters were used such as inoculum size (1, 5.5 and 10%), medium volume (1, 3 and 5ml) and incubation period (3, 4, 5 days) to observed the maximum activity of cellulase using Box-Bhenken design of response surface methodology. Maximum activity of CMCase (0.700 IU/gds/min) and FPase (0...
Tehreem Zahra1, Muhammad Irfan1, Muhammad Nadeem2, Misbah Ghazanfar1, Qurratulain Ahmad3, Shaukat Ali4, Farzana Siddique5, Zarina Yasmeen6, Marcelo Franco7
...ichoderma viride for cellulase production using seed pods of silk cotton tree as substrate in submerged fermentation by response surface methodology has been investigated. Three variables like substrate concentration, peptone and KH2PO4 were optimized at three levels. The optimum carboxymethylcellulase (CMCase) activity and filter paper cellulase (FPase) activity ...
Abdul Nabi Jatt1,2*
...duction of extracellular cellulase enzyme. Mainly, two different reporter strains such as Agrobacterium tumefaciens A136 and Chromobacterium violaceum CV026 were applied to confirm the production of signal molecules. The identification of signal molecules was achieved by GC-M...
Imrana Khushk1, Abdul Nabi Jatt2, Abdul Sattar Qureshi1*, Choudhary Haider Ali3, Muhammad Aqeel Bhutto1, Imran Ibrahim Khaskheli1, Sartar Iqbal Panhwer1
...t inhibitors and cost of cellulase enzyme). In the present study, long term evolutionary adaptation was evaluated for ethanol and lactic acid production to overcome the pretreatment inhibitors issue. Saccharomyces cerevisiae Angel was used for the production of ethanol, while, Bacillus coagulans, Pediococcus sp. and Lactobacillus sp. were used for lactic production, respectively. All the microbial strains were inoculated on wheat straw hydrolysate and were mon...
Nuraini Nuraini*, Mirzah Mirzah, Yuliaty Shafan Nur, Harnentis Harnentis
... The parameter measured: cellulase activity, crude fiber, fiber digestibility, crude protein, and nitrogen retention. The results of phase 1 showed that the types of fungi had a very significant effect (P <0.01) on the nutrient quality of fermented Azolla microphylla. Fermentation of Azolla microphylla with Lentinus edodes and Pleurotus ostreatus gave the best products. Phase 2. Application Fermented Azolla microphylla (AMF) in the diet on broiler performa...
Mubasshir Sohail*, Qadeer Ahmed Soomro, Raza Muhammad, Muhammad Usman Asif and Imran Rauf
Budi Santoso*, Bambang Tjahyono Hariadi, Marlyn Nelce Lekitoo
...o assess the impact of a cellulase additive. In the complete randomized design experiment, six treatments (silage) were used: A, B, C, D, E, and F. Treatment A consisted of oil palm frond (40%), king grass (30%), cassava (10%), tofu waste (10%), molasses (7%), lactic acid bacteria (LAB) inoculant (3%), and cellulase 0 ml/kg. Treatment B comprised silage A + 3 ml cellulase/kg, while C used ...
Sabahat Anwar1, Faria Javaid2 and Qurat-ul-Ain2*
...d industrially important cellulase enzymes by using a thermophilic fungus i.e., Chaetomium thermophilum, which can efficiently produce cellulases extracellularly while growing on cellulosic substrates. Wheat straw, by product of wheat crop has been used as lignocellulosic substrate. To determine the optimum conditions for maximum production, experiment was carried out at various substrate concentrations, temperatures and pH ...
Aisha Khalid1,2, Roheela Yasmeen1*, Farah Ahmad1, Mudassar Aslam2, Muhammad Tayyab2
...recombinant thermostable cellulase from Thermotoga naphthophila (CELTN) on the growth performance of broiler chicks when added to corn-based feed. The thermostable cellulase was produced using BL21 CodonPlus cells with the pET28a-Cellu vector under optimized conditions. A total of 150 one-day-old, unsexed broiler chicks were divided into five treatment groups, each further subdivided into three replicates of 10 chicks with a...
Abdussalam Muhyideen Zainab Funmilayo and Lee-Hoon Ho*
...ified with pectinase and cellulase and their combination, at concentration of 1.0 U/ mL for 50°C and 15 min. After that, the mixtures were all kept at 90°C for five minutes in order to pasteurize the juice. Beetroot juice without treatment: fresh juice and juice with enzyme extraction but not pasteurised nor clarified served as control 1 and control 2, respectively. The juices were evaluated for pH, total titratable acidity, total soluble solids, vitam...
Faturrahman1,3*, N.L. Nakadira1, N. Anjani1, B.N. Fajriah1, B.F. Suryadi1, A.A.M.N. Kasman2, T.W. Setyaningrum1
...se, amylase, lipase, and cellulase enzymes. The distribution was measured by isolating, counting, characterizing, and identifying Bacillus spp. bacteria in five segments of the digestive tract. The isolates were tested for their ability to inhibit the growth of pathogenic bacteria using the paper disk method, as well as for their capacity to hydrolyze protein, amylum, lipids, and cellulose. Moreover, the degree of pathogen inhibition and macromolecule hydrolys...
Safaa M. Ali1*; Nadia A. Soliman2
Novel Research in Microbiology Journal (2019), 3(6): 590-597
.../p>...
Alaa Fathalla Mohammed
Novel Research in Microbiology Journal (2020), 4(1): 641-652
...timize the production of cellulase and chitinase enzymes by plant growth promoting rhizobacteria (PGPR). Bacteria were pre-isolated from soil and identified as; Pseudomonas fluorescens NBRC (KW1), Serratia liquefaciens ATCC 27592 (EW1), Bacillus subtilis SBMP4 (EF1) and B. megaterium NBRC 15308 (TF2). The effect of different growth parameters including; pH, temperature, carbon and nitrogen sources, and incubation periods were optimized for production of
Fatma M. Abdel Baset; Noura Sh. A. Hagaggi*; Francis F. Hezayen; Usama M. Abdul- Raouf
Novel Research in Microbiology Journal (2020), 4(6): 1045-1056
...i.e., amylase, protease, cellulase, lipase and L-asparaginase. The aims of the current study were to identify the endophytic bacteria associated with the different organs of the medicinal plant C. procera, and to evaluate their potentialities to produce diverse extracellular hydrolytic enzymes....
Simiat O. Jimoh*; Semirah A. Sanusi; Farouq A. Olaogun; Ramot B. Badmos-Oladapo; Kifayat O. Asafa- Adedimeji
Novel Research in Microbiology Journal (2022), 6(6): 1821-1835
...zymes (e.g., amylase and cellulase), and mevalonate pathway enzymes, including isoprene synthase, mevalonate-5-diphosphate decarboxylase, and isopentyl phosphate kinase, in addition to isoprene, mevalonic acid (MVA), and its isomer dimethylallyl diphosphate (DMAPP). Based on the results obtained in this study, bioisoprene synthesis via direct fermentation of cheap and abundant carbon sources such as cassava peel using the S. cerevisiae 19KB strain will overcom...
Mohammed Ajdig1,2; Bahia Rached2,3; Ahlam Mbarki1,2; Taha Chouati2,4; Chouhra Talbi1; Elmostafa El Fahime2,4; Marouane Melloul1,2*
Novel Research in Microbiology Journal (2024), 8(3): 2414-2434
...tivities (i.e., lipases, cellulases, and proteases). In addition, several PGP traits such as phosphate solubilization and the production of siderophores and indol-3 acetic acid were also observed. Molecular identification through 16S rRNA sequencing initially identified all the isolates as Bacillus spp. The multi-locus sequence typing (MLST) scheme, using six housekeeping genes (adk, ccpA, recF, rpoB, sucC, and spo0A) had unveiled distinct allelic profiles res...
Tri Anggarini Yuniwati Foenay1, Theresia Nur Indah Koni1*, Agustinus Paga1, Oswilda Henyati1, Yosefus Frederikus da-Lopez2
... rumen fluid can produce cellulase enzymes that degrade crude fiber, while palmyra sap can reduce phytic acid levels in rice bran. In this study, local microorganisms (MOL NR) were sourced from a mixture of rumen fluid, palmyra sap, and sugar water, fermented for three days. This study aimed to examine the effect of different MOL NR levels on the nutritional composition and fibre fraction of rice bran fermented for six days. The study used a completely randomi...
Mikka Hakkinen1, Asep A. Prihanto2*, Abdul R. Faqih3, Septi Anitasari3, Happy Nursyam2, Royani L. Hayati4, Hefti S. Yufidasari2
...oduction of protease and cellulase enzymes, aiding in nutrient digestion. DNA sequencing identified four isolates as belonging to the genus Enterococcus, a well-established probiotic genus. These findings suggest that Enterococcus spp., identified as a lactic acid bacteria isolated from Gobiopterus sp., is a promising candidate for use as a probiotic.
Keywords | Biofilm, Enzymes, Gobiopterus sp., Grati lake, Lempuk fish, Probioti...
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