Mahmood-Ul-Hassan*, Naeem Fiaz, Muhammad Akhlaq Mudassir and Muhammad Yasin
...five sugarcane varieties/clones under varying harvesting dates of plant crop using randomized complete block design (RCBD) with split plot arrangements. The cane genotypes viz. S2003-US-704, S2006-SP-30, S2003-US-410, S2006-US-321 and HSF 240 (check) were kept in main plots whereas harvesting dates viz. 1st November, 1st December, 1st January, 1st February and 1st March of plant crop were pla...
Tong Feng, Zilu Zhang, Minghao Qu, Chan Luo, Laiba Shafique, Qingyou Liu and Kuiqing Cui*
...mparison showed that the clones were 99%, 87%, 86%, 85%, 84%, 81%, and 77% similar to the published pig, cow, human, dog, sheep, mouse, and chicken, respectively. It showed that MC1R gene had a high conserved type among different species. The goat MC1R protein has a molecular mass of 34.65 ku, an isoelectric point of 8.70, which is weakly alkaline, and contains seven transmembrane domains typical of cell membrane receptor proteins. Sequencing analysis of the b...
Muhammad Aman Ullah Khan1*, Muhammad Talha Aslam1, Abdul Rehman1, Muhammad Nawaz2, Muhammad Jahanzaib Khan1, Muhammad Ahsin Ayub3, Arbaz Ahmad Khan1, Muhammad Adil Rehman1, Bilal Ahmad Shahzad1, Sajid Hussain1, Muhammad Umair Hassan1 and Riaz Ahmad1
...ing potential of diverse clones of sugarcane under different trench spacing. The study was comprised of different trench spacing i.e., 90, 120, 150 and 180 cm and diverse sugarcane clones i.e., US-272, US-658, US-127 and US-704. The study was conducted in a randomized complete block design with split plot arrangement having three replications. The findings depicted that trench spacing and clones
Kartika Noerwijati1*, Sholihin Sholihin1, Tinuk Sri Wahyuni1, Rohmad Budiono2, Nguyen Van Minh3, Roy Hendroko Setyobudi4, Zane Vincēviča-Gaile5 and Lulu Husna6
...in this study were 1,016 clones from 42 families. Cassava selection in the early stages involved a large number of clones, therefore cluster analysis (the K-means cluster analysis method) was carried out to classify clones based on tuber yield and harvest index and to facilitate the selection process. The aim of this study wasto select high yielding cassava clones<...
Fayaz Ahmad, Noorullah Khan, Farrukh Siyar Hamid, Abdul Waheed, M. Abbas Khan, Imtiaz Ahmad, Shamsul Islam, Basharat Hussain Shah, Sohail Aslam and Qamar uz Zaman
...ea (Camellia sinensis L) clones. The experiment was laid out in randomized complete block design (RCBD) with three replication and 100 plants per replication. Data were recorded on plant height (PH), number of branches per plant (NBP), number of leaves per plant (NLP), stem diameter (SD), main root length (MRL), root diameter (RD), number of lateral roots per plant (NLRP), dry shoot weight (DRW) and dry root weight (DRW). The highest plant height (78.66 ...
Eman A. Ahmed1, Osama Y. Shalaby2, Emad F. Dwidar2, Samah A. Mokbel1, Ahmed K. El-Attar1
...equences of the Egyptian clones with each other and with the
other sequences of phytoplasma strains on GenBank showed that we may have two
different phytoplasma isolates infecting tomato plants in Egypt.
...
Sahar A. Youssef1; Manal A. El-Shazly1,2; Azza G.Farag1,3; Eman A. Khattab1,2
... sequencing revealed the clones to be portion of PNRSV genome. These results indicate that the virus in rose is an isolate of PNRSV from Taif, KSA....
Sheng Dong1*, Ning Li2, Jiawei Zhang2 and Ting Wang2
... increased the number of clones formed (P<0.05), reduced the apoptosis rate and GRP78, GRP94, Caspase 12 protein levels (P<0.05), and increased Nephrin protein level (P<0.05). High glucose-induced podocytes had increased KCNQ1OT1 expression level (P<0.05). DS reduced KCNQ1OT1 expression level (P<0.05). The inhibition of KCNQ1OT1 expression resulted increase in the survival rate and nephrin protein level of high glucose-induced podocytes (P<0....
Jie Yang1, Wenjun Zhou2, Qi Zhang2, Lei Chu2, Zhenshi Chen1, Xiajun Zhang2, Weidong Wu3*, Shaoru Zhang1* and Lihui Wang1*
...of ST1866 N. gonorrhoeae clones in eastern China....
Aadil Sultan, Mohsin Ahmad Khan*, Nadeem Ahmed, Muhammad Hassan, Rashid Bhatti, Hafsa Naeem, Muhammad Islam Khan, Saad Tahir, Samia Afzal* and Ahmad Ali Shahid
... folds as compare to the clones having single copy of gene. Gene multimerization strategy can be used to enhance production of recombinant proteins in bacterial expression system....
A.S. Gamal El din1; Sohair I. El-Afifi2; A. S. Sadik 2; Nashwa M. A. Abd El-Mohsen1; and H. M. Abdelmaksoud1
...I and Bgl II to identify clones containing inserts. To confirm the fragment inserted of CP gene sequence. PCR was performed using specific primers for PVYN CP gene. (3) The gene expression was performed using 100 µM IPTG & 2 µM biotin and incubation for 4h / 37 oC to produce biotinylated protein for E. coli with Mr 22.5 KDa and fusion protein with Mr ≈ 48 KDa consists or biotin tag and CP product or PVYN. (4) The protein was purified by s...
Hafiz Muhammad Walayat Ali1, Hafiz Basheer Ahmad1*, Zaheer Ahmad Nazar2, Muhammad Akhlaq Mudassir1, Mahmood-Ul-Hasan1, Abdul Khaliq1 and Muhammad Shahzad Afzal1
...n. One hundred and three clones of sugarcane were evaluated for two crop seasons through applying high inoculum conditions for smut incidence following RCBD during 2017-18. The analysis of variance revealed significant variations in cane weight, sugar recovery and smut incidence for both years. During the evaluation 84 and 80 clones were found resistant to smut in plant and ratoon crop respectively. Principal component analy...
Danish Kamal2, Muhammad Abbass Khan1, Ghulam Mujtaba-Shah1, Naveed Ahmed1, Maryam Iqbal¹, Basharat Hussain Shah1 and Imtiaz Ahmed1*
...or all tea varieties and clones in my study field relate to their morphological and anatomical features. Tea belongs to family Theaceae and Camellaceace. The experiment was carried out for the morphological, anatomical studies of tea varieties and clones grown at National Tea and High Value Crops Research Institute, Shinkari Mansehra. The field experiments were conduct in Randomized Complete Block Design. Morphological and a...
Aboud*, K. A; Gomaa**, Hanaa H. A.; El—Taholowy***, Mohage, A. and El - Sugher**, S.
...hoots of micropropagated clones more than healthy clone. The developed RAPD profiles of different micropropagated clones were untypical to that of the healthy clones. The phylogenetic free recorded that the plantlets derived from healthy and sanitary (10 mg/L thiouracil) plants showed close similarity within the first group while sanitary (20 mg/L) second group and sanitary (30 and 40 mg/L...
*El-Helaly, Sahar H.; ** Ahmed, Amal A.; * Awad, M.A. and ** Soliman, A.M.'
...was determined from cDNA clones. The CP gene was cloned into pGEM-T Easy vector, and transformed into Escherichia coli (E. coli) strain DH5a. The recombinant plasmids were obtained and sequenced. The nucleotide sequences were compared with corresponding viral nucleotide sequences reported in GenBank. The analysis showed that nucleotide sequence of the Egyptian isolate of AMV (GenBank Accession No. HQ288892) had percentage of similarity ranged from 97-98% with ...
Jaime Vera Chang1, Arianna Torres Coronel2, Luis Vásquez Cortez2,3, Kerly Alvarado Vásquez2,3 and Frank Intriago Flor4*
...d from five experimental clones (T1-Testigo EET-103, T2-CCAT-46-57, T3-CCAT-46-75, T4-CCAT-46-88, T5-CCAT-49-98) said varieties were obtained from the Experimental Farm “ “The Dam.” For the evaluation of the physical-chemical parameters, the following variables were analyzed (acidity, °Brix, pH, potassium, fat, protein, fiber and energy), sensory variables (flavor, color, taste, smell and texture). In the analysis of variance (ANDEVA) it ...
GPS Dhillon, Avtar Singh and DS Sidhu
...ial comprising 12 poplar clones was planted at Regional Research Station, Bathinda in semi-arid region of Punjab following randomized block design with three replications and plot size of ten trees. The data collected on diameter at breast height, tree height and volume per tree at 2, 3, 5 and 7-year age were analyzed and genetic parameters were worked out. Significant differences among clones were noticed for all growth tr...
Muhammad Tahir Laeeq
...e from Italy followed by clones from U.S.A., Yugoslavia, Turkey, Netherlands. Australia, etc. Research work conducted over a period of 15 years indicated the suitability of some clones like Populus euramericana CV-I-214, and Populus deltoides clones 63/51, 18/62, 90/60, 69/55 for large scale planting in different parts of the country....
Mahmood Iqbal Sheikh
...perfected and particular clones which would successfully grow under different climatic conditions have been identified. However, the optimum
growing space requirements of poplars still remained to be standardised. Such a study had a
special significance in Pakistan in view of the tremendous shortage of raw material for various
end uses and a final decision was required to be taken as to the spacing and the rotation on
which poplar should be grown for maxi...
Raja Walayat Hussain and M. I. Sheikh
... of two important poplar clones viz., P. deltoides cv-I-63/51 and AY-48, an evergreen clone, with two size of pits and
four spacings....
Raja Walayat Hussain and M. I. Sheikh
...owth of species/
poplar clones. Original spacing was 10' x 6' (3 x 2 m) and one year old entire plants were
transplanted at the above spacing in RCB design with four replications. First thinning was
done in the crop in autumn of 1980 but it was rather on conservative side. The crop gave a
congested appearance on visit in September 1984. Therefore second thinning was done giving a
release to the left over plants to put up better growth....
M. I. Shiekh and Raza-ul-Haq
...possible to get some new clones from USA and China in 1980. In order to compare
their performance it was decided to start a study in Peshawar in the Research Garden....
K.M. Siddiqui, M.I. Sheikh and S. Rehman
...
screening of progenies/clones. The species can be easily propagated asexually and the application of hormone had
no significant effect on rooting. The narrow and
broad-sense heritability was low emphasizing the
importance of uniform site conditions and inclusion of a
large number of progenies and clones in test
plantations. These investigations suggest that a
suitable breeding strategy would further bring about
gene...
Mohammad Iqbal Sheikh
...g season to four
poplar clones. 1-90/60, 1-72/58, 1-4/64 and 1-4/55
affected their rate of growth, it has been found that
the dose of water was insignificant. However, clones
between themselves showed different rates of growth;
significant at 1%level for dia and height and at 5%for
volume; 1-69/55 showing the overall best performance
followed by 1-4/64, 1-90/60 showed the poorest growth....
M.I. Sheikh
...d plants of some of the
clones which are represented in the populetum and other experiments, established on the
premises of Pakistan Forest Institute, Peshawar. Trees of P. euratnericana cv. 1-214, aged 10
years, were measured from Changa Manga plantation as old trees of this clone were not available in the Peshawar area.
...
Mahmood Iqbal Sheikh
...d that
different poplar clones grown under the same set of
condition yield different quantities of biomass.
...
M.I. Sheikh
...the rate of growth of 15 clones under irrigated conditions was laid
out in Bhagat Reservoir plantation over an area of 2.5 ha in February, 1975. The area, an old
shisham and mulberry plantation was cleared off all the trees and 1 - year old plants of the
following 15 clones transported from Pakistan Forest Institute, Peshawar were planted at a
spacing of 4.5 x 4.5 m in 0.5 m deep pits.
...
M. I. Shiekh-Raza-ul-Haq
...alba and four exotic clones of poplars. It was
found that there was almost linear relationship between
the depths of water applied and volume....
Mahmood Iqbal Sheikh
... In 1978 some new poplar clones were received from Texas USA fortrial
in the climatic conditions obtaining in Pakistan. The cuttings were planted in
the nursery and after one year it was decided to lay out a comparative study in
the Research Garden of Pakistan Forest Institute, Peshawar (average rainfall
300 mm)....
Mahmood Iqbal Sheikh
... 1971, a populetum of 49 clones was established over an area of 2.5 hectares in compartment No. 55. One year old plants were planted at 5 x 5 m spacing. Their performance was evaluated in 1981 and the ten clones were found to be the best amongst all clones....
Mahmood Iqbal Sheikh
...istan to select suitable clones of Poplar for different climatic regions. A recently concluded experiment near Peshawar (annual rainfall 300 mm) has shown the superiority of P. deltoides 7-63/51 over P. deltoides, 7-90/60, 7-72/51, 7-69/55, 7-18/62 and P. euramericana cv. 7-214. Since this study was located in the Northern region (Lat. 34.2° N), of the country, it was considered desirable to test most of these and some other
Mahmood Iqbal Sheikh
... of sox different poplar clones over a period of 4 years, P. deltoids 1-63/51 gave the best performance. The rest of the clones in order of merit were: P. euramericana I-214 P. deltoids I-90/60, 1-72/51 I-69/55, 1-18/62....
Abdul Aleem and Mahmood Iqbal Sheikh
...r low rooting of certain clones of P. deltoides indicates that season of planting plays the most important role in root formation in cuttings; sandy loam soil is better than sand and clayee loam; frequency of irrigation, weekly or fortnight has no significant effect
...
A. R. Beg and Mahmood Iqbal Sheikh
...few hybrid and deltoides clones for the northern and central regions and some deltoides clones for the southern parts of the country. It has been especially emphasized that due attention should also be given to cultivation techniques as well as improvement, through selection and breeding, of indigenous poplars such as P. euphratica, P. ciliata and P. alba, as the natives and their hybrids are, in the long run, ...
M. Hafeez and M. I. Sheikh
...equently, some deltoides clones were also imported which showed comparatively better rate of growth but low rooting capability, prohibiting their large scale planting. Experiments were under� taken both with cuttings as well as entire plants exposing them to a number of treatments such as time of planting, different rooting media, frequency of irrigation and position of cutting on the plant etc. and using clones like...
Asim Karim*, Anwar Ali, Bilal Ahmed Qazi, Nowsherwan Zarif and Wadood Shah
...owth value of individual clones. This difference is not only related to the development of the growth-related features, but also to the properties of the wood and the possibility of its utilization. Paulownia hybrids and cultivars have a similar appearance, but they react to their environment differently depending on the situation. The best growth (with good results) for this tree is in the Middle East and Southern Europe. These are regions where planting has ...
Safaa M. Ali1*; Nadia A. Soliman2
Novel Research in Microbiology Journal (2019), 3(6): 590-597
...H5α. The resulting clones were screened as cellulase producers using a qualitative method. The positive clones which showed hydrolysis on the plate were screened once more in Luria-Bertani (LB) medium. The plasmids were isolated and then tested using universal primer (M13), to detect the fragment size and sequence for the Polymerase Chain Reaction (PCR) products. This study establishes an effortless and professional me...
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