Johanna Lindahl 1, 2
British Journal of Virology
James Ayukepi Ayukekbong1,2
E-mail | james.ayukekbong@microbio.gu.se
...ebrile patients revealed dengue IgG seroprevalence of 73% and malaria prevalence of 80% in some areas of the country. The diagnosis of the disease is further complicated by the fact that, dengue fever mimics malaria fever and the prevalence of dengue-malaria co-infection is high. Differential diagnosis of fever is therefore necessary to initiate the appropriate clinical care. Despite the e...
Farkhanda Manzoor1, Iram Amin2,*, Muhammad Shahid2,Samia Afzal2,Hania Ramzan1, Tahir Rehman Samiullah2 and Muhammad Idrees3
... rapid identification of dengue virus from larvae of Aedes aegypti (L) and Aedes albopictus (Skuse) by using PCR. During this study, larvae of A. aegypti and A. albopictus were collected from different localities of Lahore during May to September of 2012 to 2014. RNA extracted from each mosquito larvae pool was tested by nested PCR for detection of dengue virus. Out of total 50 pools of Aedes a...
Jehangir Khan1,2*, Inamullah Khan3, Ijaz Ali2, Aqib Iqbal2 and Muhammad Salman4
...;">Despite the fact that dengue is one of the major public health threats in Pakistan causing severe outbreaks since 2005, the molecular epidemiology in mosquito (dengue vectors) is limited. Here, we investigated the vertical transmission of dengue virus (DENV) in the Aedes mosquitoes and its (DENV) transmission through other factors mainly tyres trade. DENV was detected in Aedes...
Muhammad Shahid1, Iram Amin1,*, Samia Afzal1, Zareen Fatima1, Sadia Zahid1, Usman Ashraf1 and Muhammad Idrees2
...le each year. To address dengue outbreak 2013 in Pakistan we aimed to conduct a comprehensive study at molecular level to determine the main causative serotype of 2013 DENV outbreak in Pakistan. Overall 703 serologically positive suspected patients from different major health centers of Pakistan were registered in present study of which 214 were females and 489 were male. Overall weighted prevalence of PCR positivity was 38% (268). Of the total 268 PCR positiv...
Sana Rafique1, Hina Saqib1, Khushi Muhammad2, Nazia Akbar2, Abdul Rauf3, Muzafar Shah4,*
...tients were screened for dengue virus non-structural protein using Rapid Diagnostic kit for Dengue virus (DENV) i.e. SD BIOLINE Dengue IgG/IgM. Data was obtained from patient’s record, filled forms and through questionnaire. Total 1332 blood samples were collected from 3 tehsils of District Mansehra. Out of which, 725 were found positive for dengue
Ejaz Mahmood Ahmad Qureshi1,*, Amtul Bari Tabinda2, Seemal Vehra3 and Atif Yaqub4
...sovarial transmission of dengue viruses is a crucial phenomenon responsible for persistence of the viruses during inter-epidemic period (s) of the disease. In the present study, distribution and seasonality of horizontally transmitted dengue viruses in Aedes mosquitoes was investigated in the metropolitan city Lahore, Pakistan during 2011, 2012 and 2013. Adult Aedes aegypti, were captured from nine towns and on...
Muhammad Shahid1, Iram Amin1, Samia Afzal1,*, Zareen Fatima1 and Muhammad Idrees2
...ity and morbidity due to dengue fever is increasing worldwide at a frightful rate. Emergence and re-emergence of dengue fever in previous epidemics has been documented globally, including Pakistan. We conducted a comprehensive study and compared the immunological and genomic status of dengue outbreak in Pakistan through ELISA and PCR. In this study 720 samples were collected from different...
Samia Afzal*,Sadia Zahid, Iram Amin, Muhammad Shahid and Muhammad Idrees
Hassan Shahzad Khan1, Muhammad Tariq1*, Tariq Mukhtar2 and Asim Gulzar1
Abdul Sattar*, Hasan Orooj and Muhammad Iqbal Afridi
... very beginning when the dengue epidemic had erupted first time in the year 2011. DHS, MCI/CDA adopted WHO strategy for vector control, however, a new strategic tier was additionally introduced i.e. high risk mapping and response. The team of DHS (Health) envisaged that the load of disease followed by precise micro plan/ mapping that details out distribution of disease, vector (pre-adult and adult stage), as well as sectors vulnerable for
Abdul Sattar*, Hasan Orooj and Muhammad Iqbal Afridi
... very beginning when the dengue epidemic had erupted first time in the year 2011. DHS, MCI/CDA adopted WHO strategy for vector control, however, a new strategic tier was additionally introduced i.e. high risk mapping and response. The team of DHS (Health) envisaged that the load of disease followed by precise micro plan/ mapping that details out distribution of disease, vector (pre-adult and adult stage), as well as sectors vulnerable for
Abbas M. Al-Azab1*, Ahmed A. Zaituon2, Khalid M. Al-Ghamdi3, Fahd M. Abd Al Galil4,5
Mujahid Tanvir1, Muhammad Asam Riaz1*, Muhammad Zeeshan Majeed1, Mazhar Iqbal Zafar2, Muhammad Tariq3 and Muhammad Bilal Tayyab1
...iseases such as malaria, dengue, filariasis and systemic allergic reactions in humans. Southern house mosquito Culex quinquefasciatus Say is found in tropical and subtropical regions of the world and transmits many zoonotic diseases in humans and in wild and domestic animals. It is primarily controlled by the extensive use of conventional synthetic insecticides against most of which it has developed resistance. This study was aimed at determining the toxicity ...
Ghulam Murtaza1,6*, Muhammad Irfan Fareed2, Noshaba Munawar3, Muhammad Nadeem Khan4, Gul-E- Zahra5, Taskeen Ahmad6, Muhammad Daud6 and Taqi Raza6
Nusrat Bano1, Muhammad Tayyab1*, Bushra Muneer2, Sehrish Firyal1, Abu Saeed Hashmi3, Muhammad Wasim1 and Ali Raza Awan1
...n the basis of impact of dengue infection on various clinical, hematological and biochemical parameters in dengue patients for the quick diagnosis and detection of dengue stage for the proper treatment of infection. This cross-sectional study was conducted at Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore and Jinnah Hospital Lahore (JHL) f...
IMTIAZ ALI KHAN,GUL ZAMIN KHAN,INAMULLAH KHAN
Faheem Ali* and Amjad Farooq
... the world including the dengue virus. The dominant occurrence of Aedes mosquitoes under the influence of environmental factors (temperature, relative humidity, and rainfall) of Multan city was assessed during 2014-2016. In this study, Aedes larvae were collected by using the entomological dipping method and identified in the laboratory. The results showed mean (±SD) temperature, rainfall, and relative humidity during 2014-2016 ranged 12.88-35.43oC, -0....
Rakhtasha Munir1, Shazia Rafique2*, Iram Amin1,2, Sameen Ahmed2, Ayesha Vajeeha2, Amjad Ali3, Muhammad Shahid2, Muhammad Umer Khan4 and Muhammad Idrees2
... acute hemorrhage fever, dengue shock syndrome, and liver dysfunction. Early diagnosis of the disease is crucial for prompt treatment strategies. One of the detection strategies of the infection can be made through viral structural peptides that act as markers for discernment, including Capsid (C) protein. In the proposed research, the structural gene obtained from local isolates was targeted for detection. For this purpose, a bacterial expression system was u...
Pakistan Journal of Zoology
November
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Analysis of Mitochondrial COI Gene for Species/Breed Level Identification of the Genus Capra (Linnaeus, 1758)
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Molecular Surveillance Revealed Increasing Trend of Mycoplasmosis in Respiratory Infections of Goats (Capra hircus) in Northern Pakistan
Faisal Ahmad, Farhan Anwar Khan, Midrar Ullah, Muhammad Saeed and Hayatullah Khan
Pakistan J. Zool., Vol. 57, Iss. 2, pp. 811-820
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