Manzoor Hussain*, Miloslav Zouhar and Pavel Rysanek
...(95.6%) and second stage juveniles (J2) (95.8%) infection. In greenhouse experiment, similar trend was found. L. muscarium proved to be more effective against M. hapla whereas S. rugosoannulata and C. rosea showed better results among other tested fungi in experiments. Moreover, plant growth parameters were also improved due to antagonistic relationship of fungi and nematodes in soil....
Manzoor Hussain*, Miloslav Zouhar and Pavel Rysanek
... 250 g of soil, eggs and juveniles per cyst. Nematicides effects were not quite significant among each other but had tremendous effects in reducing nematode population compared to that of untreated control. A significant (P=0.05) reduction in numbers of eggs per cyst warrants that nematicides were able to penetrate into cysts. Reproduction rate (Pf/Pi) of L. muscarium treated plants was documented minimum as compared to that of control but slightly higher comp...
Ayanda Opeyemi Isaac,1,* Oniye Sonnie Joshua2 and Auta Jehu2
...re of Clarias gariepinus juveniles to commonly used herbicides, glyphosate and paraquat was evaluated through changes in fish behaviour and mortality. Juveniles of the African catfish were exposed to varying acute concentrations of glyphosate and paraquat. The fishes responded, exhibiting different behavioural abnormalities like hyperactivity, abnormal swimming, restlessness, loss of equilibrum and haemorrhage. Observation o...
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...of galls and egg masses, juveniles and eggs per root system, and reproduction factor. Four initial populations (Pi) (500, 1000, 1500, and 2000 eggs) mixed with antagonistic fungal conidia levels (1×103, 1×104, 1×105, 1×106) were used during the experiments. The roots were stained with Phloxine B, the egg-masses were quantified, the root systems were rated for galling, and the egg masses were measured on a 0 to 5 scale, where 0 = no gall...
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...and egg masses, eggs and juveniles (J2), and the reproduction factor (Rf=Pf/Pi) was observed in the plants treated with L. muscarium compared to the control. However, all chemical nematicides were also effective in reducing the nematode infestation significantly (P=0.05) in soil compared to the control, but there was no significant difference (P=0.05) among them. Plant growth was significantly (P=0.05) improved in the plants treated with L. muscarium....
Ali Murad Rahoo1,2, Tariq Mukhtar3,*, Simon R. Gowen1, Rehana Kanwal Rahoo4 and Shaukat Ibrahim Abro5
... production of infective juveniles (IJ) of Steinernema feltiae and ability to locate hosts, therefore, in the present studies investigations were done on these aspects. A significantly greater emergence of IJ of S. feltiae from Galleria mellonella was observed with White traps than with modified Baermann trays. Maximum juveniles of S. feltiae emerged at 50 IJ dose followed by 100 IJ. The emergence of IJ decreased significant...
Abdur Rahim1, Ghulam Abbas1,*, Muhammad Naeem2, Sara Ferrando3, Lorenzo Gallus3, Muhammad Hafeez-ur-Rehman4, Abdul Ghaffar5 and Abdul Mateen6
...l (PO) were given to the juveniles for 60 days. Best specific growth rate (SGR) and feed conversion ratio (FCR) were noted in the fish fed diet containing FO. Fish fed diet comprising OO and PO showed poor SGR and FCR values than those fed with FO and SO diets. Body composition was not significantly influenced by different lipid sources although low crude lipid was found in fish fed diet containing FO. The hepatosomatic index (HSI) and visrosomatic index (VSI)...
Glomus mosseae (Gerd and Trappe) and Neemex Reduce Invasion and Development of Meloidogyne incognita
Atta ur Rehman Khan1,2*, Nazir Javed2, Shahbaz Talib Sahi2, Tariq Mukhtar1, Sajid Aleem Khan2 and Waqas Ashraf3
...d with 1000 second stage juveniles of M. incognita. Eggplants inoculated with nematodes only served as control. Each treatment was replicated tenfold. Data were recorded after one week interval up to five weeks to record different developmental stages of M. incognita. After each harvest, neemex in combination with G. mosseae proved the most effective as the development of nematode was adversely affected. Developing
Abdur Rahim1, Ghulam Abbas1,*, Sara Ferrando2, Lorenzo Gallus2 and Abdul Ghaffar3
...ght relationships of the juveniles showed significant (P < 0.0 I) growth and condition factor associated to feed application in treated ponds. Body composition of the fish was not significantly (P>0.05) different in both the treatments, though protein and lipid contents of fish in treated ponds were greater than that of control ponds. The moister content of liver, muscle, viscera and whole body were not significantly different in control and treat...
A. K. Chaubey and Satyandra Kumar
Bio-management of root knot nematode and root rot disease by antagonistic fungi and rhizobacteria
...ulated with second stage juveniles of M. incognita @ 2J2/g soil. The exposure of M. incognita eggs and juveniles to the culture filtrates showed high inhibition in egg hatching and caused high mortality of juveniles than the control (P< 0.05). The microorganisms which caused high nematicidal activity also showed antifungal activity in dual plate culture test in vitro. All the antagonist...
Hameeda Kalhoro1, Abdul Malik2,Ghulam Abbas2,*, Illahi Bux Kalhoro3, Sajjad Ali Shah4and Haleema Kalhoro3
...ing capacity each). Fish juveniles (length: 10.63 ±0.980 cm, weight: 9.030±1.40g) were stocked after acclimatization. A total of 3 treatments were tested following 2 replications i.e. first treatment (T1, Squid feed), second treatment (T2, Tubifex feed) and third treatment (T3, pellet feed). Juveniles were fed with 5% of total biomass twice in a day. At the end, higher growth in...
Naheed Bano* and Muhammad Afzal
...table diet especially at juveniles stage. This study focus on the healthy production with low cost by using sunflower meal to some extent replace fish meal. In this 3× 3 factorial and completely randomized design study 324 fish were used. The acidification levels were (0, 1.5 and 3%) whereas the phytase levels were (0, 500 and 1000 FTU). The addition of 3 % citric acid and 1000FTU phytase produced significant interaction effect on calcium and phosphorus ...
Abdul Malik1,2, Ghulam Abbas1,*, Abdul Ghaffar3, Sara Ferrando4 and Lorenzo Gallus4
Ali Murad Rahoo,1,2,* Tariq Mukhtar,1,3 Shoukat Ibrahim Abro,4 Barkat Ali Bughio5 and Rehana Kanwal Rahoo6
... production of infective juveniles (IJ) in G. mellonella was significantly affected by nematode species. Significantly higher numbers of IJ were produced by Heterorhabditid species than Steinernematid species in the cadaver. The production of IJ was the maximum in the case of H. bacteriophora which was not statistically different form H. indica. Minimum IJ were produced by S. feltiae. The IJ produced by S. kraussei and S. ...
Jaime Molina-Ochoa1, 2, 6,*, Edelmira Galindo-Velasco2, Ana María Rosales-Gutiérrez2, Martín González-Ramírez3, Roberto Lezama-Gutiérrez3, Wilberth Chan-Cupul3, Steven R. Skoda4, Muhammad Irfan Ullah5, Luis Jorge García-Márquez2 and John E. Foster6
...d the emerging infective juveniles (IJs) were collected from White traps, and pooled in canted neck vented plastic flasks. The susceptibility of an important livestock arthropod, the cattle tick, Rhipicephalus microplus Canestrinito JMO71 was determined. Ticks were manually collected from cross-bred beef cattle, and then the heterorhabditid nematodes assayed against these ticks using the Petri dish assay. Concentrations of 0, 500, 1000, 2000, 4000 and 8...
Dachuan Cheng1,2, Md Mahbubul Hassan3, Zhenhua Ma1, 2, 3,*, Qibin Yang1 and Jian G. Qin3
...hropterus larvae and juveniles from hatching to 36 day-post hatching (DPH). Mandible, ceratobranchial, cleithrum and gill arches were the initial skeletal structures appeared at 3 DPH that supported the vital life functions such as feeding and respiration. Ossification of premaxilla and maxilla and dentary started at 3.21 ± 0.25 mm (9 DPH), and completed at 5.91 ± 0.34 mm (18 DPH). The head skeleton formation completed at 22.35 ± 2.26 ...
Nazia Qamar1, Sher Khan Panhwar1,* and Ralf Riedel2,*
...portant dietary item for juveniles and adults. Adults secondarily preferred crustaceans. Fish was predominant for S. commersonnianus and crustaceans, especially of the genus Acetes sp., was equally important for S. tol. Acetes sp. was more important during the dry season for both, S. commersonnianus and S. tol. Adults of both species showed a higher feeding activity. The IRI showed fish, followed by crustaceans, to be ...
Ali Murad Rahoo1,*, Tariq Mukhtar2, Ali Murad Jakhar3, Rehana Kanwal Rahoo3
... the dosage of infective juveniles (IJ) applied and exposure periods. Significantly (P< 0.001) greater numbers of nematodes (predominantly IJ) were recovered from the cadavers of T. molitor that had been inoculated with S. feltiae than H. bacteriophora. The inoculum dose also had an influence on the numbers of nematodes recovered. There were significantly greater numbers of S. feltiae in the 50 and 500 IJ treatments than the 10 I...
Ghulam Muhae-ud-Din1,4, Anam Moosa1, Umer Farooq Ghummen1, Muhammad Jabran1, Amjad Abbas1, Muhammad Naveed2, Abdul Jabbar1 and Muhammad Amjad Ali1,3,*
...al number of egg masses, juveniles and females, were observed in highly susceptible plants. The values of canopy length, dry root weight, fresh root weight, root length, dry shoot weight and shoot height was variable for all plants and the maximum canopy length was recorded in C. macrocarpa, while maximum fresh root weight, dry root weight, root length and shoot weight were recorded in O. japonicus and R. escelsa, respectively. The suscept...
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...tion of the second stage juveniles, and the reproduction rate were detected at the highest levels in untreated soil, whereas low numbers were encountered in soils initially treated with the fungus, L. muscarium. Meloidogyne incognita and other plant parasitic nematodes multiplied significantly more in untreated soil than in treated soil, which clearly indicated that L. muscarium effectively reduced the nematode infestation level in the soil.
Ali Murad Rahoo1, Tariq Mukhtar2,*, Barkat Ali Bughio3 and Rehana Kanwal Rahoo4
... production of infective juveniles (IJ) from Galleria mellonella cadavers may differ among different species of EPN and hosts of various sizes. Therefore, the objective of conducting the present study was to compare the productivity of two EPN (Steinernema feltiae and Heterorhabditis bacteriophora) in G. mellonella larvae of different sizes. The size of G. mellonella had significant effect on the emergence of IJ. Significantl...
Ali Murad Rahoo1,*, Tariq Mukhtar2, Barkat Ali Bughio3 and Rehana Kanwal Rahoo4
...he survival of infective juveniles (IJ) following inundative applications would be quite short. The application of EPN in cadavers may be appropriate because of the non-availability of industrially produced isolates. Therefore, in the present studies, Galleria mellonella and Tenebrio molitor were compared for invasion and production of IJ of Steinernema feltiae and Heterorhabditis bacteriophora. Both the nematodes caused 100% mortal...
Naveed Ahmad1,*, P.J.A. Siddiqui1, Amjad Ali1, Khan Mir Khan2, Rafaqat Masroor3, Noor ul Akbar4, Muhammad Amin5 and Mohammad Attaullah6
Iqra Jaffar, Zainab Sehzadi, Muhammad Adeel, Khalid P. Lone and Mehwish Faheem*
...sh (Labeo rohita) juveniles were exposed to low concentrations (10μg/l and 100μg/l) of di-n-butyl phthalate (DBP) for 7 days to evaluate oxidative stress in liver and gills by analyzing activity of glutathione-S-transferase (GST), catalase (CAT), glutathione (GSH) and by measuring lipid peroxidation (LPO). Catalase activity increased significantly in liver and gills of fish exposed to 10μg/l of DBP. Level of GSH and LPO increased significantly ...
Muhammad Afzal1, Nighat Sultana1, Ali Hassan1, Syed Zakir Hussain Shah2,*, Mahroze Fatima3, Syed Makhdoom Hussain4, Muhammad Bilal5 and Majid Hussain2
...hu (Labeo rohita) juveniles fed citric acid (CA) acidified phytase (PHY) pretreated soybean meal (SBM) based diet.Basal SBM based diet was supplemented with two levels of CA (0 and 2%) and two levels of microbial PHY (0 and1000 FTUkg-1) in factorial arrangement resulting in the formulation of four experimental diets. Chromic oxide (1%) was added as an inert marker in the diets to evaluate the nutrient digestibility. Important water quality pa...
Khansa Nazir1,*, Tariq Mukhtar1 and Humayun Javed2
...The maximum mortality of juveniles was recorded at a concentration of 100 mg/ml followed by 75 mg/ml of AgNP. The minimum mortality was recorded with 25 mg/ml of AgNP. With the increase in concentration, there was a corresponding increase in the mortality of juveniles showing a direct relationship between mortality and concentration of nanoparticles. The effect of time on mortality was also found significant. With the increa...
Jae-Kang Lee, Hyun-Su Hwang, Tae-Kyung Eom and Shin-Jae Rhim*
...thly captured adults and juveniles of A. agrarius and A. peninsulae were significantly higher in the post- than in the pre-thinning session. Population sizes were dramatically increased in the post-thinning session. Ground and understory vegetation coverage and number of downed trees ha-1 positively influenced the density. The overstory vegetation coverage negatively influenced the density. Tree thinning resulted in well-developed grou...
S. Jalbani1, N.T. Narejo2,* and P. Khan2
...itiated for 90 days. The juveniles of experimental fish with mean initial body weight 6.40 ± 1.6 g were procure from local fishing community of River Indus near Jamshoro. Feed regime like live small fish (tilapia), tubifex larvae, chopped chicken viscera and dead small fish (tilapia) were used as live and prepared feed with control. It was observed that the experimental fish fed with chopped chicken viscera exhibited significantly better growth (129.10 ...
Manzoor Hussain*, Marie Maňasová, Miloslav Zouhar and Pavel Ryšánek
...masses, and second-stage juveniles (J2) and rate of nematode reproduction (Pf/Pi) and improving plant growth factors.
...
Muhammad Afzal1, Mahroze Fatima2, Aasma Qamar1, Muhammad Farhan1 and Syed Zakir Hussain Shah3,*
...s of Labeo rohita juveniles. Four experimental diets were prepared by supplementing citric acid (0 and 2%) and phytase (0 and 1000 FTU/kg) in 2×2 factorial arrangements. Total 180 juveniles (3.45±0.013) were distributed to 12 tanks having triplicate tank for each test diet. Weight gain of each replicate was recorded on weekly basis. Results showed that growth of L. rohita j...
Babar Khan1,2 *, Nazir Javed1, Sajid Aleem Khan1, Nasir Ahmed Rajput1, Muhammad Atiq1, Abdul Jabbar1, Abdul Rehman3, Anam Moosa1 and Muhammad Amjad Ali1*
...tiplication of infective juveniles (IJs) of the S. kraussei. Four different concentrations(50, 100, 200 and 500 IJs) of tested EPN speciewere used against insect’s larvae. Reproduction rate of S. kraussei was highest at concentration of 500 IJs as compared to 50, 100 and 200 IJs. Similarly, the effect of different temperatures was also studied to evaluate the efficacy of S. kraussei on insect larvae. At 25°C, S. kraussei s...
Anila Naz Soomro1*, Hiroshi Suzuki2, Sadaf Tabassum Qureshi3 and Wazir Ali Baloch1
...cted atSt. 1 only, a few juveniles of C. sakishiemnsis were recorded at St. 3, St. 4. The length frequency histograms showed the occurrence of large number of juveniles at St. 3, 4 and 5 in smaller length groups. Also C. typus ovigerous females exhibiting spawning migration to the downstream and its juvenile also migrate back to upstream after completing the larval development in Urbaru stream were also observe...
Aouicha Djebroune1*, Aissa Mokabli1, Miloud Hammache2 and Gahdab Chakali2
...ation of cysts, eggs and juveniles / kilogram of soil increased significantly in the variety Desiree compared to the initial population. A significant decrease was noticed in the variety Kondor. However, the variety Spunta did not show significance between the final and initial nematodes population. The effect of these nematodes on the tuber yield of various potato varieties was also studied. Variety Kondor produced the best yield while Desiree gave the least....
K. Abbasi 1 , R. Wick2† and D. Zafari1
Chitinase activity of biocontrol fungi cultured from the golden potato cyst nematode, Globodera rostochiensis
...he number of parasitized juveniles and eggs in water-agar. Greenhouse trials showed T. atroviridae and B. bassiana had the highest dry root weight and tuber yield....
Abida Parveen1, Sajid Aleem Khan1, Nazir Javed1, Anam Moosa1*, Khushboo Javaid1, Hina Safdar1 and Asma Safdar2
...otal number of infective juveniles/g root and soil, galls, egg masses, females were highest in MI treated plants while lowest in plants treated with a combination of MI + SK + aqueous MO + ethanolic MO after 60 days of inoculation. SK and MO has demonstrated significant potential to suppress MI infection. Therefore, further investigations should focus on their efficacy at field level to prevent the infection of MI.
...
Amna Arshad Bajwa1, Saher Islam1, Muhmmad Imran1, Karman Ashraf2, Arman Khan1, Muhammad Farhan Khan3, Imran Rashid2, Muhammad Yasir Zahoor1, Waseem Ahmad Khan4 and Wasim Shehzad1*
...itical, particularly for juveniles. Accurate gender determination is important for studying ecology and conservation biology. Therefore, a simple molecular technique, that is precise with non-invasive sampling approaches such as faeces, would be valuable. In the present study a set of molecular markers was developed exploiting the AMLx/y gene to assess gender of Punjab urial population in Kala Bagh, using faecal samples as the DNA source. In our study, among 9...
Aftab Raza Khan1, Azhar Abbas Khan2*, Javed Iqbal3, Arif Muhammad Khan4, Zeshan Hassan2, Hafiz Muhammad Aatif2 and Umbreen Shahzad2
... diet of both adults and juveniles. Chi-square test (χ2) reflected a positive relationship between adult crop contents and different calendar months and stands. The data regarding breeding biology exhibited mean incubation period, clutch size and egg weight of 16.67±1.45, 4.0±0.58, 9.56±0.33 in study (stand 12) and 13.60±0.46, 6.4±0.46, 11.44±0.58 in control area (stand 16) respectively. There was a positive...
Zhengyi Fu1,2,3,4, Rui Yang1,2,3, Shengjie Zhou1,2,3, Zhenhua Ma1,2,3* and Gang Yu1,2,3*
... Lates calcarifer juveniles has been frequently reported in hatchery practices. Huge variation of mortality in different size grade of juvenile fish has also been observed. In order to explore the effect of size heterogeneity on gene expression level of barramundi juveniles, we graded them according to body length and selected growth-related genes (GHRH, GH, GHR, IGF-I, IGF-II, MSTN-1 and MSTN-2), and immune-rela...
Liping Liu and Pengfei Liu*
...d both the sex ratios of juveniles and adults were male-biased in this roost, which possible originated from different migrating strategies with sex and age. Culmen length, wing length and tail length of male is longer than female, male is heavier than female in body mass, these differences showed slight male-biased sexual size dimorphism, the larger body size of male may have resulted from sexual selection, while pressure of natural selection may not be signi...
Salma Javed1* and Daniyal Siddiq2
... conditions second-stage juveniles (J2) mortality was recorded after 24, 48 and 72 h of incubation in concentrations of 0.25, 0.5 or 1%. Maximum mortality was achieved with ethanol extracts followed by aqueous and methanol extracts. In the greenhouse, leaf extracts at 60 days after inoculation produced reduction in root galling, egg masses, and nematode populations in plants in treated containers compared to untreated plants (control) with values increasing as...
Asma Hanif* and Shahnaz Dawar
...ling the second stage of juveniles. Other homeopathic drugs used in the experiment showed no significant nematicidal effect against M. incognita. Kent-20 and Santonine-43 at three different concentrations of 50, 100 and 75% was highly effective when used as seed treatment of okra, sunflower, mung bean and mash beans followed by drenching the soil with these concentrations of both homeopathic drugs separately, also resulted in increasing the weight and height o...
Mohammed Abdel-Mageed Abdel-Aziz Abdel-Mageed1, Eman Alsayed Hammad2*, Nashaat Abdel-Aziz Mahmoud1 and Anas Farag El-Mesalamy1
...survival of the nematode juveniles and egg-masses hatched depending on materials property, concentrations and solvents used in extraction. The aqueous extract had the best percentage of mortality and inhibition of egg hatching compared to the acetonic and methanolic extracts. Whereas, aqueous extracts of deadly nightshade, winter cherry and chili pepper at concentration 500 ppm applied to the plants as foliar sprays alone, foliar sprays with chelates and soil ...
Atef El-Sagheer1*, Anas El-Mesalamy1, Abdel-Monem Anany2 and Nashaat Mahmoud1
...hatching of second-stage juveniles (J2) of Meloidogyne javanica were estimated in laboratory and greenhouse conditions. Results revealed that all the tested culture filtrates caused significant reduction in number of J2 in all treatments, compared to the non-inoculated control, where the hatching inhibition and mortality percentages increased with an increase in exposure time. At 24 hours, increased the mean number of J2 hatched slowly than in first stages of ...
Sudip Debnath1, Dipta Sundar Sarker1, Pankaj Kundu1, Md. Shahin Parvez1, Shaikh Tareq Arafat1, Roshmon Thomas Mathew2, Yousef A Alkhamis2,3, Md Moshiur Rahman1, Sheikh Mustafizur Rahman1*
...n triplicates. Swordtail juveniles (initial weight: 0.46±0.03 g and length: 3.28±0.30 cm) were randomly stocked in glass aquaria feeding ad libitum two times a day. After four weeks of feeding trial, survival of swordtail juveniles was high (≥ 90%) in all treatments and remained unaffected by the dietary treatments (P > 0.05). Differences in growth performance were realized in terms of final weight (g), f...
Oce Astuti1, La Sara2*, Muzuni3 and Safilu4
...gs, hatch and grow to be juveniles. Therefore, BSC population stocks in this waters are continuously sustained....
Muhammad Anwar Ul Haq1*, Tariq Mukhtar1, Muhammad Inam-ul-Haq1 and Azeem Khalid2
...larly, minimum number of juveniles were recovered from roots and soils of resistant genotype (Janak) followed by moderately resistant genotype (Pala) while maximum juveniles were recovered from roots and soils of susceptible and highly susceptible genotypes. Likewise, variations were also observed in reproduction of the nematode on 21 genotypes. Minimum reproductive factor of M. incognita was observed on Janak followed by Pa...
Ikele*, Chika Bright, Ujunwa Aghaji, Mgbenka, Benard Obialo
...on on Clarias gariepinus juveniles with emphasis on the growth performance and impact on physiological status. A total of two hundred and seventy juvenile Clarias gariepinus were randomly distributed into six plastic bioreactors groups (A-F) in triplicates with equal volume of inoculum, maintained in 30 L tank with constant aeration. Addition of organic carbon sources (CN 10:1, 20:1) were evaluated to stimulate rapid growth of microbial dynamics biomass wherea...
Saima Mehar1 and Salma Javed2*
...itions. The second stage juveniles (J2) were exposed at 24, 48 and 72 hours in different concentrations of plant extracts. During in-vitro condition inhibition of egg hatchability and J2 juveniles’ mortality varied according to the concentration of plant extract. WC=1 showed 100% mortality after 48 hrs of exposure at 1% with the lethal concentration (LC50) values 0.168 mg/land all four concentration are 100 % toxic at ...
Febriza Dwiranti1, Nur Fadhilah2, Ursula Paulawati Maker2, Priyo Sambodo3*
...adult females, and three juveniles) were used in this study. Morphological observations of S. papuensis were based on body hair pattern and blood values, including erythrocyte count, leukocyte count, hemoglobin level, hematocrit number, platelet count, and leukocyte differentiation by laboratory analysis. Descriptive morphological data and quantitative data were analyzed using ANOVA testing. The adult cuscus appeared to have more spots (the male spotted patter...
Huma Abbas1*, Nazir Javed1, Muhammad Kamran2, Sajid Aleem Khan1, Abdul Jabbar1, Akhtar Hameed1, Hira Abbas3, Azhar Iqbal2 and Ehetisham-ul-Haq2
...with 500 freshly hatched juveniles (J2s) of M. incognita. Data were recorded after 7, 14, 21 and 28 days to check the invasion and development stages. All the treatments significantly reduced invasion and subsequent development of M. incognita in roots. Different application methods regarding protective and curative were used to test the efficacy of Cartap, Virtako, Cure and Azadirachtin. Results revealed that Protective application of bio and synthetic chemic...
M. M. A. Youssef† and W. M. A. El-Nagdi
...igher number of nematode juveniles in plant roots occurred. Thus, plant density, such as 4 plants if produced it promoted the number of the hatched juveniles, galls and egg-masses in roots as compared to the plant densities of 1, 2 and 3/pot. However, number of juveniles in soil decreased with increasing plant density....
S. F. A. Awad-Allah 1 and M. S. Khalil 2
.... incognita second-stage juveniles (J2) was increased as their concentrations and exposure times were increased. The LC50 of VCT ranged from 30.86 × 103 to 83.77 × 105 mg/l, while the LC50 of Nemaless® ranged from 2.69 × 108 to 3.17 × 108 cell/ml. Results from the field experiments revealed that all of the tested treatments greatly suppressed (P ≤ 0.05) the numbers of galls, egg-masses and nematode final populations. The most pot...
A. Beyan1, A. Seid†2 and H. Shifa1
...0 second-stage infective juveniles (J2) and 10 ml FOL suspension (1x106 spores per milliliter) per pot around the root rhizosphere one week after transplanting except the control which was not inoculated. The result revealed that concomitant inoculation of MJ and FOL (NF) followed by MJ first and FOL ten days after inoculation (N1F2) was found to be highly significant in reducing the tomato growth, biomass and pathogen related parameters compared to the un-ino...
A. Ghasemzadeh1, S. Jamali1†, M. Esfahani2 and H. Pedramfar1
...00 and 2000 second stage juveniles, and four sampling times (20, 40, 60 and 80 days after inoculation). After sampling, purification, identification, and population of root-knot nematode species and race were determined, and after amplification of purified population on the tomato cultivar cv. Rutgers, inoculums were sufficiently obtained. In four-leaf stage of the plant growth, the nematode inoculum levels were introduced, and photosynthetic parameters were e...
A. M. Rahoo1, 2, †, T. Mukhtar3, B. A. Bughio4, S. R. Gowen1 and R. K. Rahoo5
...ode dosage and infective juveniles (IJ) production are
available for Steinernema affine. Therefore, in the present study the effect of inoculation doses of S. affine and
extraction methods was investigated on the production of IJ in Galleria mellonella. There was significantly greater
emergence of IJ of S. affine from the Galleria mellonella using White traps than with modified Baermann extraction
M. S. Bajestani1, K. Dolatabadi2†, E. Mahdikhani-Moghadam1
...500 and 5000
juveniles were studied on susceptible tomato cv. Karoon under greenhouse condition. Although all the treatments
reduced root infections rate and significantly effect on root weight (P ≤ 0.05). It was observed that when the
nematode inoculum increased, reproduction factor also increased while extract treatments effect decreased. In
between treatments, rosemary extract has the greatest impact on the...
A. S. A. Saad1, M. A. Radwan2†, H. A. Mesbah1, H. S. Ibrahim3 and M. S. Khalil3
...r egg-mass and number of juveniles (J2) in the soil as well as plant
growth characteristics. All nematicidal treatments reduced the incidence of root-knot nematodes when compared
with the untreated check. However, fenamiphos and oxamyl were proved to be the highest chemical compostions
that decreased galls by 91.73 and 89.53% and egg-masses by 90.80 and 88.65%, respectively. Whereas, avermactin
D. A. Darban†, S. R. Gowen and B. Pembroke
...second
stage juveniles from 50 g of the soil samples from second crop than the first crop soil samples. Three weeks duration
allowed more endospores as compared to one week and two weeks treatment.
...
Istkhar† and A. K. Chaubey†
...n of 3rd stage infective juveniles (IJs) placed the
nematode in bicornutum group and differentiated from other compared Steinernema spp. The morphological
characters of 3rd stage infective juveniles and 1st generation males showed close resemblance with S. abbasi, S.
thermophilum and S. pakistanense however, the body length was varied (583mm vs 541mm, 555 mm and 683 mm,
respect...
E. Yavuzaslanoglu1†, U. Gozel2, C. Gozel2 and M. Aydogdu3
...r 72 h with 50 infective juveniles at 25
°C.
...
D. A. Darban1†, S. R. Gowen1, B. Pembroke1 and F. Hussain2
...first harvest showed few juveniles encumbered with an average of 1-5 spores and the number of juveniles without
spores very high.
...
A. M. Rahoo1,2,†, T. Mukhtar3, S. R. Gowen2, B. Pembroke2 and M. A. Rahu4
...ernema feltiae infective juveniles (IJ) from infected Galleria mellonella cadavers in
moist and dry conditions. A significantly high number of infective juveniles of S. feltiae emerged in moist
conditions as compared to dry. A positive correlation was found between the weight of the Galleria
larvae at infection and the numbers of IJ recovered in the moist as compared with dry conditions.
M. M. A. Youssef† and W. M. A. El-Nagdi
... and
hatched juveniles on roots of dry bean and number of juveniles in soil were higher on dry bean plants replacing
cutting sugar beet than those on dry bean plants replacing uprooted sugar beet within the most periods. In contrast,
plant growth parameters were higher for dry bean plants replacing uprooted sugar beet than parameters for plants
replacing cutting sugar beet.
A. M. Rahoo1,*, †, T. Mukhtar2, S. I. Abro3, S. R. Gowen1 and B. A. Bughio4
...
feltiae infective juveniles (IJs) from infected Galleria mellonella cadavers was monitored under moist and dry
conditions at 5 and 10oC. Greater numbers of IJs of S. feltiae recovered from G. mellonella cadavers kept at 10oC
than from those kept at 5oC. Likewise, significantly greater number of infective juveniles emerged in moist
conditions as compared to dry. The relationship betw...
M. T. Javeed†, A. S. Al-Hazmi and Y. Y. Molan
...ot galling and number of juveniles of M. javanica. The T. harzianum (isolate No.27) was the
most effective isolate.
...
Ahmed S.M.H. EL Roby
...nd were sprayed with EPN juveniles. Results showed that H. bacteriophora strain (EKB20) and
Heterorhabditis sp (EIK) were more efficient with higher pathogenicity and virulence in the laboratory than the
other strains and gave the highest corrected mortality percentage in the infestation (from 68.59% to 70.83%). The
LC50 were 25.42 and 45.5 IJs/ insect for the two strains, respectively.
...
S. Fatemy†1 and H. Ghasemi2
...>Developmental stages of juveniles of G. rostochiensis Ro1 were studied in the susceptible cv. Marfona and in the resistant cvs Agria, Satina and Banba in glass-house conditions. The hatching response of the nematodes to root diffusates of these cvs was also investigated. Disinfected cysts of G. rostochiensis were placed in root diffusates and the numbers of hatched second stage juveniles (J2) were counted weekly over a 6-we...
S. Ahmed†, Q. Liu and H. Jian
...itro on the second stage juveniles of cereal cyst nematode, Heterodera avenae. The isolate XZ33-3 showed significant results against juveniles (J2s) mortality followed by the isolate of XZ 17-1, XZ 24-2-1, MH 58-60-10, MH 58-60-04 and isolate MH 01-04-01, respectively. Among them four Bacillus isolates were identified on molecular basis using 16S rDNA, physiologically and biochemically and grouped as plant growth promoting b...
I. Jafri, A.A. Shahid, A. Ibrahim† and M. Atif
...ght, egg-mass, number of juveniles and number of knots per root system. Outcomes revealed that
Controlled (C1) Meloidogyne sp., Bacillus sp., (T1), Meloidogyne sp., and Bacillus thuringiensis (T2)
remarkably suppressed nematode infestations because they act as growth inhabiting agents for juveniles;
reduce gall masses, diminish knot formation on root system, toghether with increasing shoot...
A.A. Tanimola and B. Fawole1
.../div>
second-stage juveniles (J2) of Meloidogyne incognita was investigated in vitro. Extracts were tested at
concentrations of 50,000 mg/kg and 25,000 mg/kg in an experiment laid out in completely randomized design in the
laboratory. Data were collected on inhibition of egg-hatching, mortality of juveniles and analyzed using ANOVA (P
0.05). Both concen...
W.M.A. El-Nagdi, M.M.A. Youssef† and M.F.M. Eissa
... egg-masses on roots and juveniles in soil but they were rated as less affected by root-knot nematode depending
their plant growth vigor. It is concluded that younger date palm seedlings seem to require root tissue maturation
before expressing full resistance against root-knot nematode.
...
W.M.A. El-Nagdi and M.M.A. Youssef†
..., egg-masses and hatched juveniles on roots. Consequently,
they lowered rates of nematode population ranged from 0 and 0.01, respectively. However, the higher nematode
populations were supported rest crops. It is concluded that use of poor or non host crops may be beneficial for
controlling root-knot nematode population densities in intensive cropping system.
...
A.E. Ismail† and A.M. Kheir*
...v>Population dynamics of juveniles and cysts of corn cyst nematode, Heterodera zeae under stress of a cropping
sequence regime revealed that the average numbers in each stage greatly fluctuated. The density of juveniles or
cysts gradually increased with the favorable host (Giza 2 corn) to reach its peak at the crop maturity, while the
nematode population droppe...
M.A.M. El-Saedy, A.A. Mokbel*† and S.E. Hammad**
...de galls, egg-masses and juveniles/250 cc soil (93.6-
97.5%) was reached on tomato seedlings, which transplanted in soil previously cultivated with either tested garlic
cultivars. In the greenhouse experiment, values of reproductive factor (Rf) decreased as a result of garlic oil to
reach (0.07- 0.23 and 0.16-0.34) before and after transplanting of tomato seedlings, respectively. Likewise, Rf
values were sharply ...
I. Umar† and A. Mamman*
...aining 1000 second stage juveniles of M. javanica. Egg hatch inhibition and larval mortality was
observed over a period of 72 hrs. Sterilized loamy soil (4 kg) contained in 36 perforated plastic pots (30 cm dia.
and 40 cm depth) were separately mixed with 0, 30, 40, 50 and 60 g (equivalent to 0, 10 15, 20 and 25 tons/ha) of
the fruit powder. Carbofuran was applied at 0.060 g/pot. Control pots received no amendment. Two early...
F. Shahina†, K.A. Tabassum and M.A. Habib*
...PN spray @ 1000 and 2000 juveniles/ml water were assessed for mortality percentage. All four species of insects,
viz., Helicoverpa armigera, Earias insulana, E. vitella and Pectinophora gossypiella were found susceptible to
infective juveniles of the four EPN species; S. pakistanense was the most virulent EPN species. There is a dire need
to focus further research on these EPN isolates to ...
O. A. Fabiyi
...the field. Three hundred juveniles were used for the
assessment. Acetone extract coded DNLO/Me2CO caused a significant increase in plant height, number of leaves,
number of fruits per plant and fruit weight per plant as compared with Carbofuran (p<0.05). In the laboratory,
chromatographic fractions from acetone extract had the highest percentage juvenile mortality. The infrared analysis
of the fractions reveal...
W.M.A. El-Nagdi†, M.M.A. Youssef and M.G. Dawood*
..., egg-masses and hatched juveniles on roots of eggplant and number of juveniles in soil at harvest
stage compared to untreated plants significantly (p ≤ 0.05). Lower concentration of the tested materials caused
higher percentages reduction the mentioned nematode criteria. Vice versa, increase in length of shoots, dry and fresh
weights of shoots and roots occurred by higher concentration...
J. Salma and F. Shahina
... production of infective juveniles were 60 × 10 4 to 87.4 × 10 4 IJs from each larva of G. mellonella 4.2 × 10 4 to 9.8 × 10 4 IJs from each a...
A. N. Mahar, N. D. Jan†, A. Q. Mahar and S. R. Gowen
...four different infective juveniles (IJs) of entomopathogenic nematodes S. carpocapsae (All isolate, cultured at 25 °C),
M. A. Radwan†, S. A. A. Farrag, M. M. Abu-Elamayem and N. S. Ahmed
... on numbers of galls and juveniles (J2) as well as on plant growth characteristics in a glasshouse. The rate of the formulated form of oxamyl, carbofuran or cadusafos was 0.1 g/kg so...
O. A. Fabiyi, G. A. Olatunji and O. Atolani
...b(255, 255, 255);"> juveniles and eggs. The fractions were significantly effective in causing juvenile mortality. 75% concentrations of fraction being more active and showed significant differences from other concentrations. Fractions from A. boonei
MUHAMMAD MUDASSAR SHAHZAD1*, FATIMA KHALID1, MEHWISH FAHEEM2, SYED ZAKIR HUSSAIN SHAH3, SANA BASHIR1 & SYED MUHAMMAD KHAN1
Ahmed E.A. Mahgoob1, Wafaa M.A. El-Nagdi2, Mahmoud M.A. Youssef2*, Entesar H. Taha1, Mona M.S. Zayed3, Hassan Abd- El-Khair4 and Nora R.A. Saleh2
...ages of the second stage juveniles mortality increased with increasing the concentrations and exposure periods. Generally, the highest egg hatching inhibition and juvenile mortality occurred after 96 and 72hrs exposure to the suspensions of the tested isolates of P. fluorescens depending upon their concentrations and exposure periods, respectively....
Fei Shang and Pengfei Liu*
...uggest that body size in juveniles is fully developed and the yearling males display female mimicry in wing length which is an adaptive strategy to sexual selection....
Saima Mehar1, Amtul Sami2 and Erum Iqbal3*
...ing and mortality of the juveniles of root-knot nematode Meloidogyne javanica. After 72 hours, C. cyminum (MeOH) demonstrated the maximum nematicidal activity in egg hatching (15%±2.10) of M. javanica, followed by N. sativa (Ether), 22.33±6.86%, at the lowest administered dose of 0.25 % concentration. After 72 hours of treatment, C. cyminum (MeOH) and N. sativa (BeOH) had a non-significant difference in nematotoxic impact (90.0±10.3, 88.0&...
Javeria Khourshid*, Ghulam Abbas, Shahnaz Rashid, Asma Fatima* and Abdul Malik
Ahmed El-Sayed Ismail
..., population dynamics of juveniles and cysts of H. zeae under the stress of a cropping sequence regime revealed that the average number in each stage greatly fluctuated. The density of juveniles or cysts gradually increased with the favorable host (Giza 2 corn) to reach its peak at the crop maturity, while the nematode population dropped sharply when the field became fallow. Likewise, the population densities of the previous...
Sajid Ali1, Ghulam Abbas1, Shahnaz Rashid1, Asma Fatima1*, Abdul Malik1, Dilawer Ali1, Muneer Hussain Bijoro1, Ushra Batool Hashmi1, Rumaisa Abdul Rahim1, Shahid Hussain2, Kashif Ali3, Jabbar Memon4 and Javeria Khourshid1
... acclimatization period, juveniles (n=15) were distributed into each treatment hapa (T1 and T2) with two replicates. Two iso-nitrogenous diets were prepared consisting of Diet-1 soybean meal was used as main source and Diet-2 consist of fishmeal. Feed was given twice daily by 3% of total wet body weight. Results indicated a slightly higher weight gain (WG) in T2 (44.6±0.42g) than T1 (41.6±0.49g). The condition factor was non-significant (p ˃ 0.0...
Bdour Muhammed Al-Shweily1*, Jawad Bulbul Al-Zaidawi2, Muhammed Jubair Hanawi1
...ation with 100 infective juveniles/mL of nematodes (resulting in an 83.33% mortality rate for larvae and 80% mortality rate for pupae). Remarkably, the mortality rate of the treated larvae surpassed that of the treated pupae at identical concentrations of fungi and nematodes, implying that larvae exhibit greater sensitivity and responsiveness to biological control measures compared to pupae, which benefit from partial protection due to their cocoon enclosure, ...
Mahmoud Mohamed Ahmed Youssef and Wafaa Mohamed Abd - El-Hameed El-Nagdi
...e incognita second stage juveniles (J2s). The obtained results revealed that the tested extracts at 10% caused 100% nematode mortality at 72 h of exposure followed by the other concentrations with the percentages of net mortality ranging from 49 at the lowest concentration of mashed leaves to 100% at the highest concentration of mashed leaves and flowers. Under screen house conditions, the same plant parts of khella as residues at the rates of 5.0 and 10.0 g o...
Aishat Adetola Anifowose*, Nkechi Betsy Izuogu and Benoit Katchitche Sossou
...d with 2400 M. incognita juveniles at planting. Compost manure was incorporated a week before planting at 1.5 t/ha for the single treatments and at 0.75 t/ha at planting for the combined treatments. EM was applied twice at a two-weeks interval at 4000 l/ha and 2000 l/ha for the single and combined treatments, respectively. The nematode-inoculated, untreated pots and plots served as negative controls. Generally, the increase in growth and yield parameters and d...
Nusirat Aderinsola Sadiku1, Oluwatoyin Adenike Fabiyi2* and Tesleem Taye Bello3
...re inoculated with 1,000 juveniles of Meloidogyne incognita. BPL was applied at three concentrations of 100, 200 and 400 mg/ml. Results revealed that nematode infected lettuce plants treated with BPL recorded significantly higher (P< 0.05) mean number of leaves, plant height and yield compared to the untreated control. In addition, BPL at 400 mg/ml had substantially higher nematicidal effects on both the soil and root population of nematodes of L. sativa an...
Oghenebrorhie M. Oghenochuko1,4*, Olubukola T. Adenubi3, Olusola L. Ajayi3, Fakilahyel M. Mshelbwala3, Johnny O. Olukunle3, Samson A. Rahman3 and Godfrey N.O. Ezeri2
...ed to Clarias gariepinus juveniles at 200,100,25g/kg and 5, 1.5, 0.4g/l through diets and bath. Cellular immune response, humoral changes, liver and kidney function and histopathology of some visceral organs were examined. Proximate composition of the bulb was determined. Onion bulb revealed presence of carbohydrate (7.82%), protein (4.48%), crude fiber (1.68%), iron (0.5mg/l), magnesium (210mg/l), flavonoids (0.46%), saponins (0.28%), tannins (0.95%). PCV, Hb...
Shahzad Aslam1*, Amjad Rashid Kayani2, Muhammad Irfan Ashraf3, Muhammad Azhar Jameel2 and Kiran Sahar4
...ult females, sub adults, juveniles and infants. Analysis of food resources preference of five age/ sex classes of rhesus monkeys revealed that it mainly varied due to nutritional requirements and physiological conditions of monkeys. Rhesus monkeys preferred succulent foods to the non-succulents. Eight different feeding categories: arboreal, ground, provisioning, scavenging, begging, stealing, snatching and suckling were defined. A total of 540 observations wer...
Wei Li1, Xvming Qi2, Xueli Lu3, Li Yuan4 and Huaiqing Deng1*
...e hypothesized that both juveniles in group C may be males. The results show that the circadian cycle of adult females and the sex identification of juveniles in Hainan gibbons may be inferred from differences in fecal progesterone and testosterone levels....
Kouakou Yadom Yao François-Regis*, Kra Kouamé Daniel and Atta Diallo Hortense
... a control. Second-stage juveniles and eggs of M. incognita were exposed to extracts for 72 hours and 10 days, respectively. Egg-hatching inhibition and juvenile mortality were used to evaluate the in vitro efficacy of extracts. Castor leaf extracts were applied to okra soils infested with M. incognita. The gall index, gall reduction, and nematode reproduction were determined. The unhatched egg rate ranged from 49.50 to 81.60%, and the juvenile mortality varie...
Chang Guoliang1,2*, Pan Zhengjun1,2, Zhu Chuankun1,2, Zhao Haitao1,2,
Yan Zhang3, Summaya Rajput4 and Laghari M. Younis4*
Pia Amabelle M. Flores1,3, Connie Fay Komilus2, Emelyn Joy Mameloco3 and Rex Ferdinand M. Traifalgar3*
...chromis mossambicus) juveniles. Soybean meal was replaced with fermented copra meal at 25%, 50%, 75% and 100% (by weight). Five iso-nitrogenous (at 38% CP) and iso-lipidic (at 5% CL) formulated diets were fed to hybrid tilapia juveniles (0.42 g ± 0.07) following a complete randomized statistical design. Weight gain, specific growth rate (SGR), protein efficiency ratio (PER) and protein retention (PR) showed no sig...
Irsa Shafique1, Saiqa Andleeb1*, Farrukh Naeem2, Shaukat Ali3, Tauseef Tabassum4 and Tariq Sultan4
...er of cocoons (186), and juveniles (360) were recorded during 12 weeks of composting due to the high metabolic rate of earthworms. During the process of composting, pH, temperature, electrical conductivity, C: N ratio and moisture decreased till the end of the experiment and the final vermi-compost had pH (7.8), temperature (25 ˚C), electrical conductivity (2.82 mS/cm), C: N ratio (11:1), and moisture (25%). The maximum NPK content and various trace metals we...
Mohamed El-Nasharty Abdel-Aal El-Nasharty1* and Hossam Mohamed El-Sharnoby2
...arameters like number of juveniles (J2), host efficiency (RF), and gall index (GI) with 950, 1.1, and 1.0 in sequence, followed by NemaKick without significance in the first season; likewise, in the second season, the results went in the same trend, and the reduction was slightly more in the second season. Velum Prime produced the best sugar beet productivity, including top yield (16.7 tons/feddan), root yield (23.8 tons/feddan), sugar yield (4.3 tons/feddan),...
Pakistan Journal of Nematology
December
Featuring
-
Response of Soil Nematode Communities to Zero-Tillage Weed Control Practices
William K. Heve and Isaac D. Otabil
Pakistan Journal of Nematology, Vol. 43, Iss. 1, Pages 50-60
-
Microinvertebrates Associated with Freshly Harvested Vegetables Sold in Major Local Markets in Krobo Municipalities of Eastern Region of Ghana: Implications for Health Risks, Phytoparasite Dispersal, and Biosecurity
William K. Heve and Paul O. Aniapam
Pakistan Journal of Nematology, Vol. 43, Iss. 1, Pages 41-49
-
Expanding Biological Control Spectra of Entomopathogenic Nematodes to Tick Pests
Mahfouz M.M. Abd-Elgawad
Pakistan Journal of Nematology, Vol. 43, Iss. 1, Pages 09-22
-
Avian Parasitology in Captive Psittaciformes: A Study of Ascaridia spp. Infections in Gujranwala, Punjab, Pakistan
Shahzadi Sarrah Atique and Ishrat Aziz
Pakistan Journal of Nematology, Vol. 43, Iss. 1, Pages 01-08
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