Atul Sharma, Aruna Chandra Singh, Gautam Bacher, Sunil Bhand
...ingle stranded (ss) oligonucleotides (ss-RNA or DNA), which are developed by an in-vitro selection process known as “Systematic Evolution of Ligands by Exponential Enrichment (SELEX)” technique. Among the receptors available for biosensing, aptamer exhibit the advantages of high specificity, selectivity, stability, facile labelling and modification, which makes them ideal candidates for development of new biosensing applications for detection of sp...
Hussein Aly Hussein1*, Omneya Mohamed Khattab2, Shereen Mohamed Aly2, and Mohammed Abdel Mohsen Rohaim1
...leotide insertion and 12 nucleotides deletions were identified in the GPCR genes of our isolates and other LSDVs. The amino acid sequences of GPCR genes of our isolates contained the unique signature of LSDV (A11, T12, T34, S99 and P199). Phylogenetic analyses showed that the GPCR genes of LSDVs identified from ticks were closest genetically to the previously detected LSDVs from infected ruminants, indicating a potential role of Ixodid ticks for transmission o...
Yunjun Yan, Zhenming Lü, Tianming Wang, Yongjiu Chen, Jingwen Yang, Baoying Guo, Lihua Jiang, Changwen Wu and Liqin Liu*
... dollfusi was 926 nucleotides and no repeated sequences were found in this LNCR. Phylogenetic analysis of 24 cephalopoda species based on the complete mitochondrial genome showed that the O. dollfusi is most closely related to Amphioctopus aegina. These results seems to support the recent notion that O. dollfusi should be considered as synonym of Amphioctopus aegina. More morphologic and molecular evidences should be involved...
Sherin Reda Rouby
...differed in length by 21 nucleotides from those produced from SPPV either field or vaccinal strain. Among different LSD clinical samples, skin nodules and scabs are proved excellent sample material as they yield clear DNA bands that reflect the high concentrations of the virus. The results of the current study confirm the suitability of RP030 gene in differentiation of lumpy skin disease virus and sheep poxvirus in a single PCR assay without the necessity of p...
Zhuanxia Chen1, Ningping Tao1,*, Xiao Qin1 and Dayong Liu2
...uch as free amino acids, nucleotides, trimethylamine oxide and betaine. Results showed that umami was the most prominent flavor. Compared with liver, IMP is the main flavor nucleotide in meat. However, the contents of the umami amino acids and betaine in liver are much higher than that of meat. Additionally, trimethylamine oxide is mainly found in the meat. After steaming, content of IMP in meat increased significantly. Compared with steamed meat, the flavor m...
Gangchun Xu1,2, Fukuan Du2, Yuyu Wang2, Yan Li2, Zhijuan Nie2 and Pao Xu1,2,*
... (AATAAA) was present 14 nucleotides upstream of the poly(A) tail in both the PTGES2aand thePTGES2bgene. The full-length genomic DNA sequence of PTGES2a is 3,222 bp long, and composed of seven exons and six introns. The full-length genomic DNA sequence of PTGES2b is 1,908 bp long, and composed of six exons and five introns. PTGES2a was strongly expressed in the gills and liver while PTGES2b was strongly expressed in th...
Sehrish Ashraaf1, Hafiz Muhammad Tahir1, Muhammad Akram Qazi2, Shaukat Ali1*
...fference in frequency of nucleotides in COI within the members of species and within families (thymine 41.30-44.30%, adenine 24.96-27.19%, guanine 17.18-19.48% and cytosine 12.28-13.40% while GC 29.53-32.84%). Therefore, it was concluded that variation in nucleotide frequency distribution could not to bee used as a criterion for species delimitation in spiders.
...
Jinfeng Liu1,2, Yanhong Cao3, Tong Feng1, Laiba Shafique1, Chan Luo1, Peng Zhu1,4,* and Qingyou Liu1,*
...ned. It consists of 1185 nucleotides that encodes 394 peptides bond. The BMP15 gene was observed to be expressed in cumulus tissue, granular tissue, hypophysis, genital ridge and tissues of ovary and testis. Moreover, QRT-PCR results showed that BMP15 was expressed in the whole process of embryogenesis and folliculogenesis, early high level and then down regulated. It was significantly expressed higher level in COCs of middle diameter sized folli...
Ali Mujtaba Shah1,2,3, Ali Raza Shah3, Muhammad Farooque Hassan2, Muhammad Yousif2, Zhisheng Wang1*
...rals, growth factors and nucleotides and its benefits to health of animals.
...
Irfan Shahzad Sheikh, Masroor Ahmad Bajwa, Nadeem Rashid, Muhammad Zahid Mustafa, Mohammad Masood Tariq*, Majed Rafeeq, Abdul Samad, Tauseef M Asmat and Asad Ullah
...in E (200 mg/l), dietary nucleotides (100 mg/l) and DNA (100 mg/l) extracted from chicken liver were separately offered on days 1-21, 1-42 and 22-42 in experiments 1, 2 and 3, respectively. Under standard broiler management conditions, birds were divided into five treatment groups of 48 with four replicates. Commercial pre-starter feed, starter feed and finisher feed were offered on days 1-12, 13-25 and 26-42, respectively. Birds were vaccinated against Newcas...
Aftab Shaukat1,*, Sana Hanif2, Irfan Shaukat3, Muhammad Ahsan Naeem4,5, Shadab Shaukat6, Rizwan Shukat8, Shahid Ali Rajput9, Imran Shaukat10, Mubashar Hassan4, Khalid Mehmood7, Saqib Umar11 and Muhammad Ali Jamil1
...RNAs comprising of 17-24 nucleotides that, targets in silencing of one or more genes. Despite their importance as gene regulator, computational strategies are still at its initial stages. Several computer based prediction databases remain the only foundation for rapid identification of putative microRNA target. By utilizing experimentally validated targets, the search of further targets via bioinformatics tools help to predict gene target site. Keeping this in...
Mahak Fatima1, Memoona Syed1, Rabia Zeeshan2, Farah Rauf Shakoori3, Naveed Shahzad4, Moazzam Ali1 and Zeeshan Mutahir1*
...rsion of oxidized purine nucleotides to their monophosphate forms, thus preventing their incorporation into DNA and subsequently to oxidative damage. The present study explores the relationship between MTH1 gene silencing and its outcome on the growth of gemcitabine resistant breast cancer cells named MCF7-R. For this purpose, we transfected MCF7-R cells with MTH1 specific validated siRNA. Successful transfection of siRNA and consequent cytotoxic...
Ghulam Akbar1*, Ali Ahmad2, Neha Arooj1, Muhammad Anjum Zia1, Aamna Rafique1, Sania Riasat1, Mohsin Raza1, Mahpara Qamar1, Shahneela Nusrat1 and Shakila Hanif1
Critical Update for the Treatment of Anemia by using Advanced Genome Editing Crispr Cas Technology
..., whereas different oligonucleotides are provided to make accurate DNA template for genome correction. It is of importance to knowledge about types of CRISPR to treat anemia. Cas 9 showed one of best gene editing reactions than others. Recent data gives authentic applications of CRISPR use in anemia which provides potential for usage of gene editing technique for different types of anemia. In evaluation we defined currently developed genome editing device upda...
Yongyun Zhang1, 2, Xinyang Fan1, Fangting Zhou1, Weizhen Li3, Yina Ouyang1,4 and Yongwang Miao1*
...gth, which contained 645 nucleotides and encoded a peptide composing of 214 residues. A total of 5 single nucleotide polymorphisms (SNPs) was identified in two types of buffalo. Among them, c.516T>C and c.578C>T were observed only in river buffalo, while c.175A>G, c.580T>C and c.609T>G were found only in swamp buffalo. The c.175A>G, c.578C>T and c.580T>C were non-synonymous, which led to substitutions of p.I44V, p.L178S and p.F179L. The...
Hanaa A. Elsamadony1, Laila A. Tantawy1, Sabry E. Omar2 and Heba A. Abd Alah1
...dentity with
nucleotides of Indian and Japan strains, 97% amino acid identity with the same strains. Our circulating
strain has low nucleotides identity 37%, 38% and amino acid identity 15% for both with vaccinal
strains (Cux-1N/Germany and CAV/Nobilis®P4) respectively. The high percentage of similarity of
nucleotides identity with Egypti...
Hadia Gul1, Abdul Haleem Shah1, Ricardo Harripaul2, Anna Mikhailov2, Ejaz Ullah Khan3, Wasim Shah3, Nisar Ahmad3, John B Vincent2,4 and Muzammil Ahmad Khan3*
...er includes few intronic nucleotides or skip exon-20 in the mature mRNA. Our study further supports the evidence of high incidence of OCA2 gene mutations in Pakistani families....
Ty Nguyen, Giang Thi Huong Phung, Lan Le Thuy Hoang and Giang Van Tran*
...e ratio of four types of nucleotides is A: 23.38%; T: 32.62%; G:18.15%; C: 25.85%. The rate of difference in CO1 sequences of the 2 samples is low compared to previous studies. The similarity coefficient of M. cunnesius in the two areas of Tam Giang lagoon is very high (0.9953).
Novelty Statement | The paper presented detailed morphological features and genetic diversity of Moolgarda cunnesius (...
Istkhar† and A. K. Chaubey†
...d comparative account on nucleotides analyses based on ITSs study confirmed the
present specimens as isolates of S. abbasi. The nucleotide composition resemblance within 5.8S gene with other
Steinernema species evident the less polymorphism and highly conserved nature of this region as compared to ITS1
and ITS2 and delimit the relationship of steinernematid nematodes.
...
Ayesha Ahsan1, Rabia Arif2*, Samina Nazir1, Muhammad Saleem2 and Memunna G. Shahid1
...netic variations in four nucleotides were observed for PKC gene i.e. C (150) T; C (186) A; C (429) G and T (521) A which were common for S1, S2 and S3 strains, while point mutation C (497) G was detected only in S2 and S3 strains. Post translational modifications (PTMs) of both proteins were predicted and compared with the reference sequences of Neurospora crassa and Sordaria macrospora by using different PTMs predictor servers. Phosphorylation and glycosylati...
M. A. Abou El- Nasr1, Kh. A. Dougdoug1, Hayam S. Abdelkader2, and Rehab A.
Dawoud2
...sing fluorescent dideoxy nucleotides showed that the capsid protein region of PPV-EA strain had about 65% sequence homology with other strains of PPV and 45% similarity to the CP or PPV-D strain. A PCR fragment coding for the 43 C-terminal amino acids of the Nib and the N-terminal part of the CP (complete variable region plus 19 amino acids of the conserved core) was cloned and expressed into the pQE- 100 plasmid vector. Upon induction, the viral protein coat ...
M. A. Amer1; M. H. El-Hammady2; H. M. Mazyad1; A. A. Shalabyl and F. M. Abo-El-abbas
...was determined to be 801 nucleotides in length encoding a deduced 267 amino acid. Nucleotide sequence analysis revealed a range of 96- 99.5 % sequence identity among the PVYNTN isolate (PVY-T, PVY SWS, PVY II and PVY NTN)....
Om-Hashem M. EI-Banna1, M. A. S El-Kady2, E. A Salama1 and Salwa N. Zein2
...R of RNA γa as six nucleotides were changed. 12 out of 162 amino acids were differed and one amino acid deletion. Gl19 strain cross reacted specifically with the monoclonal antiserum raised against the coat protein of ND18 stain and the two coat proteins were indistinguishable in their sizes....
Hemeida, A. A., Osman, M., El-Shahat, Mohamed, Hashem, Medhat H., Mahmoud, Amal and Dahi, Hosni
...ons were observed in the nucleotides (74, 92, 112, 113, 133, 172, and 180). Nucleotide insertion was seen in one sequence in the nucleotide position of 212. Root neighbor-joining (NJ) tree revealed that the three HCV samples could be classified as genotype 4. Two of the three samples were closely related (98.2%) while the third clustered with isolate from Gabon (98.7%). The third isolate may be a new subtype but this will require an in-depth exploration on seq...
Hend E.M. Elsheikh1*, Mamdouh F. El-Mekkawi1, A.A. Abou-Zaid1 and Amal M. Abd El-Raof2
...ealed the presence of 27 nucleotides that were absent in Romanian MEVAC-SPV and MEVAC-LSDV. So the conventional PCR targeting the partial EEV Glycoprotein gene is a quick and precise method for identifying LSDV. Also, the Partial sequence of EEV Glycoprotein gene has the ability to perform DIVA approach when use MEVAC LSD vaccine. In contrast it’s not capable to do the same on Al-Abbasya LSDV vaccine due to unlikely presence of 27 <...
Hend E.M. Elsheikh1*, Mamdouh F. El-Mekkawi1, A.A. Abou-Zaid1 and Amal M. Abd El-Raof2
...ealed the presence of 27 nucleotides that were absent in Romanian MEVAC-SPV and MEVAC-LSDV. So the conventional PCR targeting the partial EEV Glycoprotein gene is a quick and precise method for identifying LSDV. Also, the Partial sequence of EEV Glycoprotein gene has the ability to perform DIVA approach when use MEVAC LSD vaccine. In contrast it’s not capable to do the same on Al-Abbasya LSDV vaccine due to unlikely presence of 27 <...
