Jiandong Wu, Miao Ye and Zaigui Wang*
...effective extraction and purification of total flavonoids from corn silk, and the anti-hyperlipidemic activities was analyzed in rabbits by feeding experiment. Firstly, the high extraction yield (5.32 mg·g-1) of total flavonoids were obtained under the optimal extraction conditions, which are 80°C for 3 h with corn silk over 60% ethanol ratio of 1:30. Then, the AB-8 resin was selected for the purification
Amtul Jamil Sami1,*, Madeeha Khalid1, Rehman Shehzad1, Sana Mughal1 and A.R. Shakoori2
... was used to monitor the purification steps, and protein band was located by immunoblotting technique. Bubaline placental lactogen was purified to homogeneity and showed a molecular weight of about 30 kD. Purified placental lactogen was administered to male albino mice for up to eight weeks for growth promoting activity. Increased mammary growth was observed in test female non lactating mice, as compared to controls when placental lactogen was applied. Increas...
Anum Rafique, Sajid Abdullah, Khalid Abbas, Huma Naz* and Wardah Hassan
...lled conditions. Partial purification of SOD was done by Ammonium sulphate precipitation. Ion-exchange column chromatography was used to purify enzyme for further partial characterization against different range of temperature and pH. As a result of ion exchange chromatography enzyme from kidney of control and Pb+Cr treated fish had highest activity as 508.33 and 427 UmL-1 while highest specific activity was 1105.06 and 1055.55 Umg-1 with...
Habib-ur-Rehman1, Masood Ahmed Siddiqui1,*, Abdul Qayyum1, Arifa Bano1 and Naeem Rashid2
...Escherichia coli and purification of metal ion independent type II pullulanase from Pyrobaculum calidifontis. Thermophilic origin and metal ion independency of the enzyme reported in this study make it a potential candidate for starch hydrolyzing industry.
...
Muhammad Shahbaz Aslam1, Iram Gull1, Zaigham Abbas2,* and Muhammad Amin Athar1
...pression and to make its purification process more convenient.
... 
Sana Rana1, Sajid Abdullah1, Huma Naz2* and Khalid Abbas1
....41 Umg-1). Highest fold purification of CAT was noted in the gills of control C. striata (1.52±0.01) as compared to exposed fish (1.48±0.01). The percentage recovery of CAT for control and DM+END exposed fish was calculated as 52.21±1.41 and 43.10±1.41, respectively. Results further indicated that after each step of partial purification total protein contents and percentage recovery decreased fro...
Hafiz Muhammad Umer Qaisar1, Tanveer Ahmad2, Muhammad Rizwan3*, Muhammad Saqib1
Antimicrobial efficacy of combination of lincomycin and spiramycin (Lispiracin™) as systemic dry cow therapy for controlling bovine mastitis
...tion, identification and purification of microbiological
cultures. The mean score of surf field mastitis test of G1 group before treatment
and at day 14thpost calving was significantly different (p<0.05) as compared to G2
group Somatic Cell Count was significantly reduced from 8.0 at dry off to 3.50
(P<0.05) after day14th post calving in lispiracin™ group. Quarter wise prevalence
(%) of clinical mastitis in G1 gro...
cultures. The mean score of surf field mastitis test of G1 group before treatment
and at day 14thpost calving was significantly different (p<0.05) as compared to G2
group Somatic Cell Count was significantly reduced from 8.0 at dry off to 3.50
(P<0.05) after day14th post calving in lispiracin™ group. Quarter wise prevalence
(%) of clinical mastitis in G1 gro...
Aisha Khalid1, Muhammad Tayyab1,*, Abdual Rauf Shakoori2, Abu Saeed Hashmi1, Tahir Yaqub3, Ali Raza Awan1, Muhammad Wasim1, Sehrish Firyal1, Zaheer Hussain4 and Munir Ahmad5
...als with the production, purification and characterization of recombinant thermostable cellulase from Thermotoga naphthophila. PCR using the genomic DNA of T. naphthophila as template resulted in amplification of 1 kb cellulase gene. The amplified cellulase gene was cloned in pTZ57R/T and sub-cloned in pET28a. The expression of recombinant cellulase was analyzed using BL21 CodonPlus (DE3) cells as expression host. The expression studies resulted ...
Huma Naz1,*, Sajid Abdullah2, Khalid Abbas2 and Muhammad Anjum Zia3
...ays was evaluated. After purification, specific activity of enzyme was 1750.13 and 1272.72 U mg-1 in kidney of metal exposed and control fish, respectively. The enzyme from metal exposed kidney was 2.68 fold purified with 58.73 % age recovery. Results of characterization showed that SOD had broad range of pH from 4 to 8.5 but show maximum activity at pH 7 and 7.5 for stressed and control fish, respectively. At 30°C activity of SOD was maximum fo...
Zahra Nazir1, Saba Ijaz1, Roquyya Gul2 and Mahjabeen Saleem1,*
Fatima Tufail1, Sajid Abdullah1, Huma Naz2*, Khalid Abbas1, Laiba Shafique3
...ique. Results of partial purification demonstrated that the exposure of insecticides mixture decreased the hepatic CAT activity in fish (43.33±1.41 UmL-1) as compare to control (50.33±1.41 Unit mL-1). After desalting specific activity of hepatic CAT remain lowest in exposed fish as 134.30±0.71 Unit mg-1in relation to control (148.02±0.41 Umg-1). The fold purificati...
Lin Qi, Lu Li, Danyang Chen, Mingxiao Liu, Yan Wu and Wei Hu*
Sana Aslam1, Wayne Thomas Shier2 and Imran Sajid1*
...t organisms. The partial purification and metabolite profiling using ultra performance liquid chomatography and mass spectrometric (UPLC-MS) analysis suggested the presence of a variety of bioactive compounds including totarol, kifunensine, talaromycin and clausine F. These results indicated that Kallar Kahar Lake harbors a significant diversity of actinobacterial strains which are able to produce a diverse range of biotechnologically and pharmaceutically pote...
Kanwal Nisa1, Sadaf Ashraf1, Masood Ahmed Siddiqui1*,Naila Taj1, Habib-Ur-Rehman1, Arifa Bano1 and Naeem Rashid2
...s is the first report on purification and characterization of a POR/PDC from T. kodakaraensis.
...
Sikander Ali* and S. Wajiha Khalid
...;0.39 U/ml). The partial purification of the enzyme was done with ammonium sulphate (20-80 %) followed by dialysis which resulted in 21 % purification from the crude enzyme extract. The molecular weight of enzyme was found to be 86 kDa, as determined by SDS poly-acrylamide gel electrophoresis.
...
Shikun Ge1, 2, Yanli Yang3, Brindha Chelliappan4, 5, Antonysamy Michael5, Fagang Zhong1 and Xiaoying Zhang1, 2, 3, 4*
...psin, four different IgY purification methods (PEG extraction, water dilution method, the effect of liquid egg yolk and egg yolk powder preparations) on the activity of IgY antibodies was investigated. The results showed that IgY was stable below 70 oC, and the activity was significantly lost when it exceeded 70 °C; IgY was relatively stable in the range of pH 4-10, and the activity was significantly lost beyond this range; IgY had certain resis...
Imrana Khushk1, Abdul Nabi Jatt2, Abdul Sattar Qureshi1*, Choudhary Haider Ali3, Muhammad Aqeel Bhutto1, Imran Ibrahim Khaskheli1, Sartar Iqbal Panhwer1
...ase does not need enzyme purification, packing and transportation. Hence, the production of bioethanol and lactic acid cost reduced at large extent by using on-site produced cellulase in the saccharification of lignocellulosic material.
Novelty Statement | In house cellulase production saved enzyme production, purification, and transportation cost. Evolutionar...
AdlyAbd-Alla1,2, MahaNada3, Francois Cousserans1, and Max Bergoin1
...v>
of 25 nm. Virus purification on cesium chloride gradient showed one band at a buoyant
density of 1.362. Extraction of nucleic acid from purified virus particles revealed a ~9 kb
ssRNA linear genome and analysis of capsids showed two major proteins of 31 and 32 kDa
and a minor 78 kDa protein. Infection of third instar nymphs by feeding on contaminated food
induced similar disease symptoms and bioassay exp...
Ayman S. El-Habbaa1 and Mervat E. Radwan2
...-PCR) and subsequent gel purification of the amplified products of G gene of BEF virus.
Results: BEF virus was circulating in this region. Sequence analysis of G-gene of this Egyptian strain,
comparing with sequences of BEF virus circulating globally and regained from GenBank, showed
100% nucleotide homology with BEF virus from Egypt 2014 but nucleotide and deduced amino acid
substitution generates were showed wi...
Bingjie Zhou1, Hitesh Bhagavanbhai Mangukiya1, Siva Bharath Merugu1, Fakhar-un-Nisa Yunus1, Yuchen Fan1, Zhenghua Wu1,* and Dawei Li1,2,*
Mubasshir Sohail*, Qadeer Ahmed Soomro, Raza Muhammad, Muhammad Usman Asif and Imran Rauf
Syeda Rubab Zaidi* and Safdar Ali Mirza
...tion of laccase. Partial purification was accomplished through Ammonium-Sulphate precipitation and for further purification gel filtration and anion exchange methods were used along with protein estimation. The purified fraction was then subjected to enzymatic characterization to find its thermal stability, effect of pH and selected chemical compounds. Other significant experimental findings were; optimum incubation period w...
Diagnosis of Staphylococcus aureus Infection in Bovine Mastitis using its Affinity Purified Fraction
Ehab A. Fouad1*, Khaled A. Abd El-Razik2 , Eman H. Abdel-Rahman3
...rbent Assay (ELISA). For purification of fraction, bacterial fresh cultures were centrifuged at 3000 rpm / 20 min and were homogenate in phosphate buffer saline and centrifuged at 15000 rpm / 20 min that known as the crude antigen. It entered to affinity column chromatography [Cyanogen Bromide-Sepharose 4B (CNBr-Sepharose 4B)] (Sigma Chemical Co.), obtain the fraction. In brief, IgG of cows S. aureus positive sera were purified by precipitation with 50% ammoni...
Xiangli Dong1,2, Shilin Mikhail Borisovich2, Jiji Li1,*, Jianyu He1, Zeqin Fu1, Yingying Ye1, Julia N. Lukina3, Olga V. Apalikova4 and Jianshe Zhang1
Zahra Naz1,2, Fouzia Ismat1, Muhammad Saleem2, Mazhar Iqbal1, Aamir Shehzad1 and Moazur Rahman1,2*
...ide (LDAO), enabling the purification of the detergent-solubilized M protein in a soluble form through affinity chromatography without compromising the structural integrity of the protein. Using the purified M protein as a diagnostic antigen, an indirect enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of anti-NDV antibodies in multiple serum samples collected from different poultry farms of district Faisalabad, Pakistan. The data...
Iram Amin1,2*, Shazia Rafique1, Nadeem Ahmed1, Muhammad Shahid1, Samia Afzal1, Tahir Rehman Samiullah1, Mohsin Ahmed Khan1 and Muhammad Idrees1,3
...s regard. Expression and purification of HDAg, which is the only protein of HDV of the local isolate is the primary objective of the study. This antigenic recombinant HDAg protein can be useful for both vaccine development and as a diagnostic marker of HDV. After determination of HDAg antigenic region of HDV and its amplification, the fragment was cloned in bacterial expression vector. The expression of recombinant protein was checked by SDS-PAGE and after the...
Shabana Ehsan1*, Aneela Riaz2, Muhammad Amjad Qureshi1, Abid Ali1, Ifra Saleem3, Muhammad Aftab3, Khalid Mehmood4, Fakhar Mujeeb1, Muhammad Asif Ali1, Hina Javed1, Fraza Ijaz1, Anwar-ul-Haq5, Khaliq-ur-Rehman3 and M. Usman Saleem4
A. Ghasemzadeh1, S. Jamali1†, M. Esfahani2 and H. Pedramfar1
...lation). After sampling, purification, identification, and population of root-knot nematode species and race were determined, and after amplification of purified population on the tomato cultivar cv. Rutgers, inoculums were sufficiently obtained. In four-leaf stage of the plant growth, the nematode inoculum levels were introduced, and photosynthetic parameters were evaluated at different times. The results showed that by increasing levels of nematode inoculum,...
Muhammad Shahid Nadeem*, Jalaluddin Azam Khan and Firoz Anwar
...inal recovery, 15.3 fold purification and 139 U/mg specific activity. Maximum enzyme activity was found at pH 8 and above 90C, its KM and Vmax values were found 25µM L-alanine and 149 U/min/mg respectively. In silico studies have shown that the enzyme was found in a monomer structure. Molecular docking studies with potential molecules involved in the reaction catalysed have been conducted and binding energy values were calculated for each molecule includ...
Cuiping Liu
...mbined with hybrid blood purification treatment in patients with maintenance hemodialysis (MHD). 106 patients with MHD were treated in our hospital from February 2017 to January 2020. They were divided into observation group (n=53) and control group (n=53) using a random number table. The control group was given heterozygous blood purification, and the observation group was given alpha keto acid combined with heterozygous bl...
Characterization and Molecular Detection of Tomato spotted wilt Tospovirus Infecting Tomato in Egypt
Hayam S. Abdelkader1; Gamalat M. Allam1; T. A. Moustafa2; and M. El-Hanunady2
...technique for biological purification and electron microscopic analysis confirmed the production of mature infectious virus particles underlining the conclusion that a full infection cycle was completed in this system. Both the structural viral proteins nucleoprotein (N) and the envelope glycoproteins GI and G2 and the nonstructural viral proteins NSs and NSm were accumulated to amounts sufficient for detection and cytopathological analysis. Electron micrograp...
M.A.S. El-Kady1, Om-Hashem M. El-Banna 2, E.A Salama2, Salwa N. Zein1
... different procedures of purification were performed to purify a local isolate Of Barley stripe mosaic Hordevirus (BSMV). The 1st procedure was successful in producing relatively high yield of virus preparation 3.7 mg/100 g of barley leaves with adequate purity and one light-scattering zone was round in sucrose density-gradient column. The 2nd and 3rd procedures yielded aggregated virions (2.3 and 7.0 mg/ 100 g of fresh barley leaves, respectively). Electron m...
Iram Liaqat1*, Noor Muhammad1, Muhammad Mubin2, Najma Arshad3, Tehreema Iftikhar4, Sumera Sajjad5 and Farzana Rashid5
.... Further extraction and purification of bioactive components from these bacterial may be a good source of novel antibiotics and natural-insecticides....
Saad Tahir, Nadeem Ahmed*, Mohsin Ahmad Khan, Muhammad Akram, Rabia Abbas and Kausar Malik
... was carried out for the purification of the HSA-SK fusion protein. SDS-PAGE, western blot, and RP-HPLC analysis were also conducted, which confirmed the 98% purity of the product with 40% yeild having a molecular weight of almost 111kDa. The purified fusion protein (HSA-SK) was also verified via western blotting by using anti-streptokinase antibodies. Clot lysis assay was used to estimate the biological activity of HSA-SK. For half-life estimation, proteolyti...
Fariha Javaid1*, Zahoor Qadir Samra1, Madeeha Shahzad Lodhi1,2, Aroosha Hussain1 and Gulnaz Pervaiz1
...tions. The isolation and purification cost of the enzyme is highest, so there is a need to develop cost-effective purification methods. This research work aims to purify BRM by using magnetic nanoparticles. After purification, BRM enzyme was characterized and utilized in the detergent industry due to its stability at a wide range of pH and temperature; results showed that stem BRM has the ...
Khatab, Eman A.H.; Zein, Salwa N. and Ahmed, Amal A.
S. A. Sidaros*, S. A. EL-Kewey*, Eman A. H. khattab**, M. M. ELsharkawy*
Siqiang Li1,2, Tiantian Wang1, Peng Sun1, Airong Gao1, Xin Gong1, Yuanhong Xu2, Baogen Wang2, Jun Wu1* and Bo Liu1*
...s purified in three-step purification and identified by peptide mass of fingerprint. The enzymatic activity and optimal reaction conditions of MDS I were detected using DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis. We obtained MDS I with a purity exceeding 90% in gram scales, which was capable of digesting α-1,2 linked mannose residues in high selectivity. The highest enzymatic activity of MDS I occurred at a pH of 7.0 and a t...
Sijie Jian1,2,3,4, Wei Sun1,3, Jia Chao1,2, Na Rong1,4, Xiang Liu1,2,3,4* and Chen Chen1,2,3,4*
... cloning, expression and purification, and the expression conditions were optimized. In mice immunized with Slp, the immune-related factors of LZM and AKP were enhanced (p < 0.05), and a specific antiserum titer (1: 3200) was obtained that had immune recognition effects for A. hydrophila and P. fluorescens. Passive immunization of Carassius auratus with Slp mouse serum and challenging with bacteria showed a passive protection rate of Slp serum against A. hy...
Tanveer Jilani1*, Irfan Khan2, Asmat Salim2, Fareena Bilwani1, Bushra Moiz3 and Mohammad Perwaiz Iqbal1,4
... method of isolation and purification of CD34+-derived EPCs used in this study is simpler, user friendly and is more efficient as compared to other techniques. Human EPCs differentiated by this protocol can then be used to study normal and abnormal erythropoiesis....
Qurrat ul Ain Shafique1, Sana Batool2, Hanfa Ashfaq2, Asima Tayyab2, Roquyya Gul3 and Mahjabeen Saleem1*
...ction time. A denaturing purification scheme (Immobilized metal affinity chromatography) was employed for the purification of IL-2. The refolding of the purified IL-2 was achieved by stepwise dialysis method using urea gradient (8M-0M). Human IL-2 was recovered from 1 litre culture to a purity level of ~95%. In vitro cytotoxic potential of IL-2 on HepG-2 and MCF-7 cell lines revealed that it possess sufficient cytotoxi...
Ahmed Kareem Kadhim Al-Wasmee1, Waddah Salam Hassone2, Hawraa Talib Al-Janabi3, Hamed AH Al-Jabory1
...action. This genomic DNA purification was used following the instructions provided in the kit to extract the parasite DNA. The results revealed that the rates of infection of calves with T. anullata in Babylon Province were 36/90. The infection rate of T. anullata was recorded for females (47.5%) and males (34 %). Results showed that the highest infection rate (62.5%) was recorded at the <1 M, (39.1%), at 1-2 M, (20%) at 2-3 M, (22.7%) at the 3-4 M, and the...
M. N. Ashiq
...n into consideration for purification of the lines.
With this method six pure lines, four of Chinese origin
and two of Japanese origin, were evolved. All these
lines were stable and bred true for the two characters.
They were all suitable for rearing in the spring season....
S. M. A. Shah, A. Razzaq and M. T. Younis
...s, B-hydrolysis and acid purification were examined in these samples....
Indah Nurfauziah1, Lovita Adriani1*, Rahmad Fani Ramadhan1, Andi Mushawwir1, Safri Ishmayana2
...tween treatments. In the purification of WSPE yogurt, both B1 and B2 each produced five peaks, namely peaks 1 and 2 (class II bacteriocins), peaks 3 and 4 (class I bacteriocins), and peak 5 (free amino acids). The results of the bacteriocin activity test showed that B1 had higher bacteriocin activity than B2. The result of the current reaserch show that giving WSPE yogurt, both B1 and B2, has not been able to provide a significant difference in reducing feed c...
Zahraa M. Mahdi
...omising future for water purification processes. ...
Syeda Rubab Zaidi* and Safdar Ali Mirza
... centrifugation. Partial purification was carried out by ammonium sulphate precipitation method and further purification was done by column chromatography and gel filteration method. It has been concluded that locally isolated strain A. niger was one of the potential and promising fungal isolate, screened for laccase enzyme activity, from soil sample collected from agricultural area near Harbans Pura, Lahore.
H.A. Abdul-Ratha1*, Sahar Mahdi Hayyawi2, Essam F. AL-Jumaily3
...epharose 6B for acidocin purification was carried out. The effectiveness of pure acidocin was tested against culture of Staphylococcus aureus that produced biofilm of 3 mm. The study included the use of the Electron microscope to investigate the mode of acidocin and biofilm action on the bacterial cell membrane. The acidocin product indicated that pore formation important for bactericidal activity against cell membrane with highly potent for the treatment agai...
Abdul Razak1, Imran Altaf1*, Aftab Ahmad Anjum1, Ali Raza Awan2 and Farhat Nazir Awan3
...ired extra step of virus purification from its NSPs. Present project was designed to optimize different parameters of size exclusion chromatography (SEC) for the purification of local isolate of FMDV from NSPs. For this purpose Bio-rad Econo-Column 15/50 was packed with SephacrylTM S-300 resin and fitted with BioRad BioLogic LP Chromatographic system. Mixture of blue dextran and bovine serum albumin used as biomarkers for FM...
Waithaka, P.N.1*; Mwaura, F.B.1; Wagacha, J.M.1; Gathuru, E.M.2; Githaiga, B.M.2
Novel Research in Microbiology Journal (2019), 3(3): 351-365
...d techniques followed by purification, test for antagonism against selected pathogenic microbes, and cytotoxicity assay using Brine shrimp lethality test. Totally, 138 actinomycetes isolates were obtained from all the soil samples. Four isolates showed the highest potent potential against selected bacterial and fungal pathogens. The selected actinomycetes were coded; PAN 30, PAN 37, PAN 41, and PAN 154, and preserved at 4°C for further analysis. The differ...
Muhanna M. Alshaibani1*; Noraziah M. Zin1*; Juriyati Jalil2; Anis Rageh Al-Maleki3,4; Nik M. Sidik5
Novel Research in Microbiology Journal (2020), 4(2): 704-713
...;
Extraction and purification of ribonucleic acid (RNA) from Gram-positive methicillin resistant Staphylococcus aureus (MRSA) is problematic, because the MRSA has a rigid cell wall that contains lipoteichoic acid and peptidoglycan, thus causing difficulty when utilizing the standard methods. For this reason, the aim of the current study was to improve and modify the method of extraction of RNA from MRSA, with good integrity, purity, low cost, and with ...
Naam Fadhil Abbas Al-Helli*; Jehan Abdul Sattar Salman
...iv>
and subsequent purification achieved an efficiency of 99.6 %. Characterization assays of the
purified mannan included FT-IR; FE-SEM, carbohydrate content estimation, solubility, and
melting point tests, which revealed the presence of α-1,6 and α-1,2 linked sugars; crystalline
nature, high porosity (80 %) carbohydrate content, high solubility in water, and a melting point
of 248 °C. The p...
Basma T. Abd-Elhalim*
Novel Research in Microbiology Journal (2022), 6(6): 1783-1800
... on the Nile's raw water purification and sanitation in the Warraq al Hadar area, Egypt, using Moringa oleifera leaf powder. The water turbidity, pH, and total fecal-coliforms were assessed. The water turbidity and pH were decreased on addition of M. oleifera leaf powder at 2, 4, 6, 8, and 10 μg\ ml; with reductions of 49.8, 68.7, 87.4, 92.0, and 93.7 % for turbidity, while the pH was reduced by 12.37, 20.0, 28.14, 38.14, and 38.14%, respectively. Using the...
