Nayle Maria Oliveira da Silva1*, Ricardo do Carmo Zanella2
...y of patients with viral hepatitis C, whereas chronic infection is established in 60 to 80% of all cases, 20 to 40% of which may evolve to cirrhosis and hepatocarcinoma. Genetic differences among infected hosts can determine the progression and outcome of the virus infection, causing different individuals to respond in different ways to the viral infection.
...
Oladipo Elijah Kolawole*, Oloke Julius Kola, Awoyelu Elukunbi Hilda, Olasunmibo Oluwatobi Gabriel, Ogunyale Tolulope Temitope
E-mail | koladipo2k3@ yahoo.co.uk
...sk of developing chronic hepatitis in immunocompromised individuals usually leading to liver cirrhosis. Individuals living with Human Immunodeficiency Virus have a greater risk to the virus due to their impaired immunity. The study was carried out to determine the sero- prevalence of Hepatitis E among people living with Human Immunodeficiency Virus in Ogbomoso. The blood sample of consented two hundred and seventy five (275)...
Nasar Khan1,2, Fahad Ali3,7, Sulaiman Bahadar5,*, Khalil ur Rahman3, Abdul Aziz3, Noor ul Akbar4, Muhammad Daud6, Azam Hayat5 and Mujaddad ur Rehman5
...logical agent of chronic hepatitis B, hepatocellular carcinoma and liver cirrhosis. A total of 659 HBsAg ELISA positive patients out of 16825 patients during July to October, 2013 were screened for HBV by real-time polymerase chain reaction. Out of 659, 522 (79.21%) were found positive for HBV, of which 318 (over all 4.4%) were male, while 341 (over all 3.55%) were female. The HBV PCR positivity rate was 80.18% (n=255) in males and 78.30% (n=267) in females pa...
Aneeza Jamshed1* and Syed Amir Gillani2
... lack of awareness about hepatitis C due to poverty, involvement of quacks and less penetration of mass media. To develop the awareness of hepatitis C among primary grade students. A total of 150 students of primary grade among 300, of Government sector Middle School Lahore were selected through multistage cluster sampling. A quasi-experimental study was conducted in the time period of 6 months from April 2014 to Sep 2014. D...
Muhammad Rasool1,*, Muhammad Zahid1, Muhammad Ismail Khan1, Qadeem Khan2 and Khalid Khan1
Ahmad Farooq1, Usman Waheed2,3, Hasan Abbas Zaheer2,3, Abdul Rauf4, Abida Arshad5, and Muhammad Arshad1,*
... diseases, most commonly hepatitis B and C. The aim of current study was to determine the prevalence of HBV and HCV infections among thalassaemia major patients. The study was conducted from June – December 2016, at the Thalassaemia Centre, Shaheed Zulfiqar Ali Bhutto Medical University, Islamabad and the Pakistan Thalassaemia Centre, Pakistan Baitul Mal, Islamabad, Pakistan. Data were obtained by clinical testing of 1,440 ß thalassaemia major pati...
Jehangir Khan1,*, Rasheedullah1, Bakht Tarin Khan2, Ayaz Ahmad3, Muhammad Salman4 and Khalid Khan5
...iduals are infected with hepatitis and the disease is constantly increasing. Every 10th Pakistani is believed to be suffering from viral hepatitis. It is the first study documenting the epidemiology of HCV in District Shangla, Khyber Pakhtunkhwa (KPK). Blood samples from 900 individuals of various age groups were collected (August to November 2013) from patients at clinics, houses and pharmacies belonging to three...
Muhammad Shahbaz Aslam1, Iram Gull1, Zaigham Abbas2,* and Muhammad Amin Athar1
...tions such as cancer and hepatitis with its immune modulating properties as well as through its direct action on target cells. The recombinant expression of some proteins in E. coli produces inclusion bodies which are misfolded proteins. The objective of this study was soluble expression of IFNα2-Tα1fusion protein in E. coli using pET-SUMO vector and determination of its biological activity. SUMO-IFNα2-Tα1 was successfully...
Abdul Wajid1,*, Asma Basharat2, Muhammad Akbar Shahid3, Sidra Tul Muntaha1, Abdul Basit4, Tanveer Hussain5, Muhammad Farooq Tahir6, Muhammad Azhar7, Masroor Ellahi Babar1 and Shafqat Fatima Rehmani2
...ated with inclusion body hepatitis (IBH) and hydropericardium syndrome (HPS) in chickens worldwide. Identification of fowl adenovirus (FAdV) serotype is of most importance in epidemiological studies of disease outbreak and the adaptation of vaccine strategies. In spite of appropriate administration of vaccination, FAdV outbreaks have been reported and caused significant losses in poultry flocks throughout Pakistan in recent years. To identify the serotype and ...
Muhammad Saqib Shahzad1, Amina Arif1,*,Saeeda Kalsoom2, Javed Iqbal3, Shahnam Shafique2, Muhammad Shahid Nadeem4 and Rafique Ahmed5
...erferon (IFN) therapy in hepatitis C virus (HCV) patients. Overall, 590 HCV patients were evaluated pre and post combined drug therapy during the period 2015-2017. Distribution of HCV genotypes, age groups, viral load and alanine aminotransferase (ALT) values were recorded and analyzed for rapid virologic response (RVR), non-rapid virologic response (NRVR), sustained virologic response (SVR) and non-sustained virologic response (NSVR). Among the evaluated pati...
Attaur Rahman1, Sadeeq ur Rahman1,*, Ashfaq Ahmad2, Abdul Sajid1, Muhammad Asif Hussain1, Mian Muhammad Salman1, Niamat Ullah Khan1, Kamran Khan3, Fazli Rabbani1, Muhammad Akbar Shahid4, Irshad Ahmad5 and Shakoor Ahmad6
...ricardium, hepatomegaly, hepatitis, nephritis and spleenomegaly. Altogether, albeit, FAdV-4 caused similar symptoms and lesions in ostriche, however, mortality remained comparatively lower....
Shaikh Abdul Lateef, Zhou Lin, Wu Jian, Junjie Qian, Jonathan Hartanto Tan and Zheng Shusen*
..., complete recovery from hepatitis is not achieved. Epidemiologically, it has been classified into infectious and serum hepatitis. The pattern of disease progression manifests liver inflammation, chronic hepatitis and hepatocellular carcinoma. In this article, we present a comprehensive review characterizing the viral architecture, life cycle, infectivity mechanisms, immune escape strategi...
Nida Zia1, *, Ayesha Maqbool2, Muhammad Safdar3, Umm-I-Habiba1, Altaf Mehmood4, Muhammad Usman5, Zahid Iqbal6, Javed Iqbal6, Shahid Mehmood6, Amanullah Khan7 and Sajid Umar8
...iv>...
Gul Afshan1,2,*, Soumble Zulfiqar2, Sumaira Mehboob2, Muhammad Tahir Javed Khan1 and Abdul Rauf Shakoori2,*
...nder development against hepatitis C, though an effective hepatitis C vaccine is yet to come. The present study is focused on the development of recombinant vaccine against HCV by utilizing HCV3a envelope glycoprotein E2. HCV3a E2 gene was amplified and cloned first in cloning vector pTG19 and subcloned in expression vector pET21a. E2 protein, expressed in insoluble form, was purified by repeated sonications, followed...
Tahir Iqbal1, Umer Rashid1*, Naveed Shahzad2, Amber Afroz1, Muhammad Faheem Malik3 and Muhammad Idrees4
...e RNA virus causing hepatitis-splenomegaly syndrome (HSS) in chickens. To the best of our knowledge the circulation of aHEV in chicken has not been studied in Pakistan so far. Therefore, in the present study, we aimed to isolate and identify aHEV from layer chickens of Pakistan. The bile fluids, liver and spleen tissues were collected from overnight dead layer chickens (n = 8) from Pattoki region of Punjab province, Pakistan, during July to August 2016. T...
Shaikh Abdul Lateef, Zhou Lin, Wu Jian, Junjie Qian and Zheng Shusen*
...nts with pre-established hepatitis B infections. Information gained through this study would be helpful to have better management and life style in these deadly infections. ...
Muhammad Siddique1, Muhammad Shafique2,*,Abdul Mateen3, Naveed Shahzad4, Bilal Aslam2, Nasir Ahmad1 and Muhammad Usman Qamar2
Tanweer Kumar1, Sahib Zada2,3,Muhammad Irfan4,*, Huma Batool5 and Wasim Sajjad2,6
...amples were subjected to hepatitis B surface antigen Immuno-chromatographic test (ICT), HBsAg enzyme-linked immunosorbent assay and by Reverse transcription polymerase chain reaction for the existence of antibodies against HBV. It was observed that 307 (12.8%) out of the 2382 individuals harbored antibodies in their blood against HBV. Among the different age groups, the highest number of incidences of HBV antibodies was found in the 16-25 age groups (7.01%). I...
Bamigboye Olutoyin Omolara1, Fapohunda Funmilayo Omotoyosi1, Oladipo Elijah Kolawole1,2, Oni Mattthew Olujenyo1, Ajibade Oluwatosin Akinola1, Kaka Mary Oluwatosin1*
Farheen Shafique1, Shaukat Ali2,*, Saiqa Andleeb1, Abdul Rauf1,3, Syed Ayaz Kazmi1,3, Sadia Idrees4, Faisal Farooq1,3, Faiq Nawaz Khan3,4, Muhammad Saad-ul-Hassan1,3, Raja Awais Mumtaz1,3, Saba Khalid1, Zaheem Ashraf1, Hafiz Muhammad Tahir2 and Fazal-ur-Rehman5
... 350 million patients of hepatitis B and 170 million patients of Hepatitis C are present worldwide according to WHO. Many risk factors are involved in the transmission of theses deadly viral infections but blood transfusion in Beta thalassemic patients is working with two faces, one as remedy and the other is key risk factor in the spread of silent killers. Thalassemia patients registered in Combine Military Hospital (CMH) R...
Joseph Anejo-Okopi1*, Ocheme Julius Okojokwu1 and Onyemocho Audu2
Muhammad Shahzad1, Arif Iqbal Umar1, Syed Hamad Shirazi1, Muhammad Tariq Pervez2*, Zakir Khan1, Waqas Yousaf1
HCDP: HEPATITIS C DATA BANK OF PAKISTAN
...d circular in shape. The hepatitis C virus is substantial threat to the public health and its frequency is increasing rapidly all over the world. Approximately 5 online databases are available related to Hepatitis C virus. All these databases mainly concerned their national level/specific demographic region strains for analysis. No HCV database available to find the HCV prevalence and distribution in Pakistan....
Parakriti Gupta1, Kapil Goyal2 and Mini Pritam Singh3*
Hepatitis E Virus and Zoonosis
...elf-limiting acute viral hepatitis which occurs in apparently healthy individuals to acute liver failure which leads to high mortality among pregnant women. Recently, chronic infections due HEV genotypes 3 and 4 have also been documented in immunosuppressed and transplant patients where prolonged virus shedding has been documented. The conventional diagnosis is established by detection of HEV antigen or anti-HEV IgM in acute sera. However, the detection of HEV...
Ayesha Alam1,* and Labeeb Ali2
Jia Shi
...n HBeAg negative chronic hepatitis patients is necessary as they are vulnerable to developing cirrhosis and hepatocellular carcinoma. The present study was aimed to evaluate the response rate of treatment by Peginterferon alfa-2a. In this systematic review and meta-analysis, a review of articles evaluating response rate to Peginterferon alfa-2a was done through the databases of Scopus, Google Scholar, Pubmed (inception - 2019). Fourteen studies reporting the r...
Nosheen Basharat1, Usman Waheed2,3*, Muhammad Arshad1, Noor e Saba4, Iram Masood1, Akhlaaq Wazeer1, Ahmad Farooq1, Sadaf Moneeba1, Abdul Rauf5 and Hasan Abbas Zaheer2,3
...nd its relationship with hepatitis B and hepatitis C seropositivity. The study was conducted at the Department of Pathology and Transfusion Medicine, Shaheed Zulfiqar Ali Medical University, Islamabad, from November 2016 to June 2017. Total 282 samples were selected after routine blood screening of Hepatitis B and C, of which 75 were HCV positive (asymptomatic), 75 were HBV positive (asymp...
Joseph Anejo-Okopi1*, Jennifer Ifeoma Okpara1, James Mogoret Dabol1, Joshua Adetunji1, David Adeniyi2, David O. Amanyi3, Otobo I. Ujah4, Veronica David1, Patience Omaiye5 and Onyemocho Audu6
...ally, over 20 million of hepatitis D virus (HDV) are coinfected with Hepatitis B virus, which has an estimate of 350 million infected individuals. The study aimed to determine the prevalence of HDV infection in HBV/HIV and HBV-mono infected patients in Jos, Nigeria. This cross-sectional study was conducted on ninety (45 HBV/HBV and 45 HBV-mono) infected patients with chronic hepatitis B at...
Samina Tahir Kiani1, Abdul Rauf1, Syed Ayaz Kazmi1,*, Nuzhat Shafi1, Madiha Khalid2, Naeem Latif1, Samina Gul2, Saghir Yousaf3 and Muhammad Arshad4
...ctor regarding spread of hepatitis B and C in the studied population.
...
D. N. Abd-elshafy1, 2 and M. M. Bahgat2, 3
Mai A. Kilany1; Hanaa H.A. Gomaa1; Yousry E. Abo El-magd2 and Nermin Raafat2.
...causes acute and chronic hepatitis in humans with a high propensity for chronicity. If untreated, chronic hepatitis C can progress to cirrhosis and hepatocellular carcinoma (HCC) in a subset of patients. HCC is a prevalent disease in many populations worldwide. It causes many financial problems in treatment modalities with high mortality rates. In develop-ing countries, the major concern in HCC frequently belongs to HCV long...
Amal Mahmoud1&2 and Medhat H. Hashem3
Serageldeen Sultan1, Nabila Osman2
... the prevalence of avian hepatitis E virus (avian HEV) among chicken flocks in Egypt. A total of 510 serum samples were collected from broiler breeder (n=191), layer breeder (n=235) and commercial broiler chickens (n=84) from different farm flocks (24 flocks) in Egypt. These samples were examined for the presence of avian HEV antibody using enzyme linked immuno-sorbent assay (ELISA). The results revealed that the occurrence of avian HEV was 15.7% (80/510) and ...
Ayesha Muzamil, Hafiz Muhammad Tahir, Shaukat Ali, Iram Liaqat, Aamir Ali, Muhammad Summer
...gy, autoimmune diseases, hepatitis, coeliac disease, inflammatory bowel disease and glomerulonephritis are linked directly or indirectly to different inflammatory processes. Conventionally, different steroidal and non steroidal drugs i.e., antibiotics are used to treat inflammatory disorders. Theses synthetic drugs have many side effects on the health such as gastrointestinal problems, stomach ulcers, dizziness, liver or kidney problems etc. Cytokines play an ...
Muhammad1, Saadat Mehmood1,2, Atif Muhammad1*, Zaib Ullah3
...patitis B and hepatitis C viruses. The present study is designed to assess the frequency and transmission of (HBV) and (HCV) among the local population of district Swabi. A preliminary study was conducted from 1st January to 31st May 2016. The study revealed that the ratio of Hepatitis B and C in the local population was found 103 (34 %) and 199 (66 %) respectively. Out of 143 males, 37 (26 %) were infected by HBV...
Saima Parveen1, Altaf Mahmood2*, Ayesha Azad3, Sajid Umar4, Nosheen Shoukat6, Mirza Muhammad Arsalan Azam5, Qurat-Ul-Ain5 and Nausheen Akhtar Malik1
... (7.87%), inclusion body hepatitis (7.79%), chronic respiratory disease (7.67%), necrotic enteritis (6.48%), coccidiosis (6.09%), mycotoxicosis 5.43%), fowl cholera (4.74%), infectious coryza (4.41%), fowl typhoid (4.22%), omphalitis (3.71%) and hydropericardium syndrome (0.05%). Maximum share in crude morbidity was contributed by bacterial diseases with highest proportional morbidity of 48.68% followed by viral (40.32%), parasitic (5.80%) and fungal (5.20%) d...
Tahir Munir Butt1*, Azra2, Muhammad Luqman3, Naveed Farah4 and Muhammad Yaseen3
Jun Zhang1*, Xiaocao Xu2 and Min Cao2
... viruses that among them hepatitis B and C viruses are in high significance. This study investigates the seroprevalence of HBV and HCV in hospital admissions for thalassemia major in Bengbu, China. This study is a cross-sectional study in which information was extracted and collected from patients’ records, including gender, age, number of blood transfusions per year, HBs-Ag, HCV-Ab and Anti-HBs. The study included 630 thalassemia major patients who rece...
Noora Hassan Hezam Al-Aqmer1*, Zain Aamir2, Muhammad Farooq Hanif3, Soumble Zulfiqar4, Sibgha Zulfiqar1, Mateen Izhar5 and Abdul Rauf Shakoori4*
...hism and the response to hepatitis C directly acting antiviral treatment. This study which is a case control study included 66 hepatitis C patients (genotype 3) who responded to the directly acting antiviral treatment and achieved negative HCV-RNA three months after completing the treatment (sustained virologic response (SVR)) and 66 hepatitis C patients (genotype 3) who did not achieve SV...
Amreen Zahra1, Mushtaq A. Saleem1*, Hasnain Javed2 and Muhammad Azmat Ullah Khan3
A. El-Shemy1*, Hoda M. Mekky2, M. A. Bosila2, A. M. Allam1, Kh. M. Elbayoumi2, M. M. Amer3
...s ...
Iram Amin1,2*, Shazia Rafique1, Nadeem Ahmed1, Muhammad Shahid1, Samia Afzal1, Tahir Rehman Samiullah1, Mohsin Ahmed Khan1 and Muhammad Idrees1,3
Mansha Tahir1, Abdul Rauf2,3, Syed Ayaz Kazmi2,3*, Nuzhat Shafi2, Muhammad Hasnain Ali Gillani3, Sundus Khawaja4, Syeda Maria Fiaz Bukhari4, Tehniat Summer4 and Muhammad Majid Bashir2
...ermine the prevalence of hepatitis B and C infection among the general population of union council Sena Daman district Jhelum Valley, Azad Kashmir and to assess the risk factors for their transmission. From approximately 16000 total individuals of population of Union Council Sena Daman 710 (4.4%) individuals were randomly screened for hepatitis B and C prevalence using Immuno-Chromatography Kits, ELISA and further confirmed ...
Muhammad Shahid1*, Muhammad Idrees1, Afza Rasul2, Iram Amin1 and Samia Afzal1
Ubaida Hussain1,2*, Amtul Jamil Sami1*, Shazia Rafique3*, Muhammad Idrees khan3 and Ahmad Ali Shahid3
A. A- E. Abo-Elkhier1, K. S. E. Abdel-Wahab2, M. A. Ali3, M. A-H. El-Sayed4, N.A-K. Saleh5 and A. A. Atef6
Mohamed Mahrous Amer1*, Hanaa Sayed Fedawy2, Hoda Mohamed Mekky2, Khaled Mohamed Elbayoumi2, Ahmed Ali El-Shemy3, Mohamed Abd El-Rahman Bosila2
...es, pericarditis and perihepatitis were prominent E. coli infected with hepatic subcapsular hemorrhage mostly in S. aureus groups. Hematological parameters were mostly affected in all infected non-treated groups compared to negative control without significant difference. Histopathological changes of infected non-treated groups showed inflammation of s.c tissue with massive heterophils and mononuclear cell infiltration, hydropic degeneration of the hepatocytes...
Samia Afzal1*, Jahanzaib Ahmad1, Iram Amin1, Liaqat Ali2, Muhammad Shahid1, Mohsin Ahmad Khan1 and Muhammad Idrees1
... is a satellite virus of hepatitis B virus (HBV) as HDV relies on hepatitis B surface antigen (HBsAg) for viral development and release from hepatocytes. It causes the most severe viral hepatitis and suppresses the serological and molecular markers of HBV infections; for example, Hepatitis B envelope antigen (HBeAg), inhibition and reduction of HBV DNA t...
Rimsha Ijaz, Rida Zahra, Naseer Ali Shah*, Haroon Ahmed, Nazish Bostan and Sadia Sattar
... The principal causes of hepatitis C disease transmission in Pakistan, a developing country with a high incidence rate, are unscreened blood transfusions, unsterilized surgical instruments, and unsafe injections. Globally a total of about 170 million people have been afflicted with this malady per year and affects approximately (6%) of Pakistan’s population. HCV is RNA virus and there are seven primary genotypes and 67 subtypes in it. Determination of ge...
Shafi Muhammad1, Bibi Nazia Murtaza2, Aftab Ahmad1, Muhammad Shafiq3, Nurul Kabir4 and Hamid Ali1*
...ctive feature of chronic hepatitis C infection is the presence of persistent liver inflammation characterized by the expression of different inflammatory molecules including Interleukin-17 (IL-17). The progression of chronic liver ailments (fibrosis, cirrhosis) towards hepatocellular carcinoma (HCC) is determined by the distortion produced in the normal structure of liver. Pakistan bears the second highest numbers of hepatitis
Hemeida, A. A., Osman, M., El-Shahat, Mohamed, Hashem, Medhat H., Mahmoud, Amal and Dahi, Hosni
...of group A. Three random hepatitis C virus (HCV) samples from group B were studied for the diversity and sequence variations. Sequencing of 223 nucleotide of 5'-untranslated region (5'-UTR) was performed. In the phylogenetic analysis, the three sequences and 80 sequences of HCV different genotypes from GenBank were used. Nucleotide variations were found in 7 (3.14%) of the 223 nucleotide positions analyzed. The variations were observed in the nucleotides (74, ...
Elkalamawyl, I.M.; Elhddadl, S.; Swelim2, M.A.; Hamdy2, S.M. and Fahmy3, Hanan A.•
...Two novel mutants of the hepatitis B virus Surface antigen (HBsAg) were characterized. The mutants were isolated from asymptomatic patients who were found to be persistently positive for both I-BsAg and anti-hepatitis B surface antibody (anti-HBs) and a long-lasting anti-HBc (core) IgM. Polymerase chain reaction (PCR) and sequencing were performed to surface antigen region and revealed two genotype D mutants 1--1BV. Aligned ...
Abo Elmagd l , Enas K.; Abdel-Wahab l , Kouka S.; Alrasheedy l , Zeinab E. and Khalifa2, Ahmed S.
...ims to assess markers of hepatitis C Virus (HCV) including HCV antigen detection in peripheral blood mononuclear cell (PBMNC) lysates, antibodies to HCV core, NS3, NS4 and NS5 epitopes by enzyme linked immunoassay (ELISA) in mothers and their infants using serum samples for both mothers and infants plus saliva for infants, in addition to detection of serum HCV- RNA (viremia) by reverse transcription polymerase chain reaction (RT-PCR). Also detection of antiHIV...
El-Tabakh, SAA l ; Abdel Wahab, KSE; Badr, AF and Helal, IG
...gn: justify;">Genotype 4 hepatitis C virus (HCV) in viremic serum was used for experimental infection of a human hepatoma cell line (HepG2) at a multiplicity of infection (MOL) of one genome copy per 103 cells. Stock HepG2 cell cultures were grown in Dulbecco modified minimum essential medium (DMMM) as growth medium with 10% fetal calf serum and antibiotic mixture (IOOU penicillin and 100 mgm streptomycin/ml) and subcultured at 3-4 days. HepG2 cells were maint...
Fadia M. Attia and Hanaa H. A. Gomaa
...in patients with chronic hepatitis C. Aim of the study: to describe different patterns of changes accompanying viremic status in HCV chronic liver diseases. 50 patients with chronic hepatitis C were enrolled in this study. Qualitative and quantitative PCR by real time PCR was done to determine the level of viremia before induction of therapy. In addition, complete blood count, prothrombin time and liver function tests were p...
Joseph Oyiguh Abraham1, Cornelius Arome Omatola1*, Martin-Luther Oseni Okolo1, Ruth Foluke Aminu1, Emmanuel Edegbo1, Olubunmi Marvelous Emurotu1, Danjuma Muhammed2, Jesse Joseph Chock3, Helen Ojomachenwu Ocean4 and Yahaya Ocholi5
...odeficiency virus (HIV), hepatitis B virus (HBV), and hepatitis C virus (HCV) remain a significant public health concern globally. However, there is a dearth of information about the prevalence of the three viruses in the study area. Therefore, the current study determined the prevalence of HBV, HCV, and HIV among students of a tertiary institution and its environs in Idah, Kogi State, Nigeria. Sera obtained from 1000 consen...
TAHIR IQBAL1, 2, UMER RASHID1* & MUHAMMAD IDREES3, 4
...ed as causative agent of hepatitis-splenomegaly syndrome (HSS) in chickens. Like other HEV strains, it is single-stranded positive sense RNA virus belonging to Hepeviridae. In the present study we report partial structural homology of ORF3 encoded protein (ORF3P) of Pakistani novel aHEV (Pak naHEV) strain with a kinase 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophos-phokinase (HPPK) which participates in folate biosynthesis in bacteria. This finding...
MUHAMMAD NAEEM RAZA1, KALSOOM SUGHRA1* & NADIA ZEESHAN1
SHEIKH AJAZ RASOOL1, MUHAMMAD SALMAN RASOOL2 & MUNAZZA AJAZ3
... 20% people die of viral hepatitis in one of Pakistan provinces. A counter malarial acrine drug is being tried against proteinaceous infectious particles (causing many transmissible neuro-diseases). These epigenetical agents have been a source of concern for our planet regarding food safety issues (e.g. infected meat). No doubt, man has made significant achievements in effective and neo-antimicrobials research, one wonders why not a single infectious agent has...
Abdul Ghaffar1*, Kashfa Akram1, Habiba Jamil1, Riaz Hussain2, Ghulam Abbas3, Fozia Afzal4, Ahrar Khan5,6, Rabia Tahir7, Muhammad Ahmad Chishti1 and Shahnaz Rashid3
... respiratory infections, hepatitis, restlessness, and chest tightness, were significantly more prevalent among pesticide-exposed individuals. Hematological parameters revealed significant decreases in hemoglobin, white blood cell count, erythrocytes, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), lymphocytes, and neutrophils, alongside significant increases in mixed cell count and...
Iqra Almas, Samia Afzal*, Muhammad Idrees, Muhammad Shahid, Iram Amin and Kausar Malik
... an accurate analysis of hepatitis B virus (HBV) as compared to Sanger sequencing. In 2019, samples from HBV infected family of mother and her two sons samples were collected from Lahore, Pakistan. Sequencing of S gene, Geno2pheno (HBV) 2.0 tool and GHOST platform were used to analyze the genotype, mutations (natural and escape) and quasispecies analysis of the HBV. All samples in family were infected with genotype HBV/D1 and had high viral load (10e8 IU...
Saima Younas and Aleena Sumrin*
...n has the second highest hepatitis C virus (HCV) prevalence with seroprevalence of 4.5-8.2%, with genotype 3a being most frequently circulating HCV genotype. Current treatment strategies for HCV are based on direct acting antivirals (DAAs), despite high efficacy of antivirals, still therapy failure has been reported in 5-10% cases due to resistance mutations of amino acids. This study was aimed to analyze clinically important resistance associated mutations (R...
