Characterization of Unusual Human Group A Rotavirus VP4 Genotype Detected in Moroccan Children Fully Vaccinated with Rotarix™
Hassan Boulahyaoui1,2*, Sanaa Alaoui Amine1,3, Marouane Melloul4, Farida Hilali5, Elmostafa El-Fahime1,3, Saad Mrani1,2 and Nadia Touil1,5*
1Centre de Génomique des Pathologies Humaines (GENOPATH), FMPR, Mohammed V University in Rabat, Av. Mohamed Belarbi El Alaoui, B.P.6203 10000, Morocco; 2Centre de Virologie, Maladies Infectieuses et Tropicales, Hôpital Militaire d’Instruction Mohamed V, Angle Avenues des FAR et Allal El Fassi, Hay Ryad, B.P. 8027 N.U, Rabat, Morocco ; 3UATRS, Centre Nationale Pour la Recherche Scientifique et, Angle Avenues des FAR et Allal El Fassi, Hay Ryad, B.P. 8027 N.U. Rabat 10102, Morocco ; 4Laboratoire de Physiologie, Génétique et Ethnopharmacologie, Faculté des Sciences, Université Mohammed Premier, BV Mohamed VI BP 717, Oujda 60000, Morocco; 5Laboratoire de Recherche et de Biosécurité, Hôpital Militaire D’instruction Mohamed V, Angle Avenues des FAR et Allal El Fassi, Hay Ryad, B.P. 8027 N.U, Rabat, Morocco.
ntouil2003@gmail.com, Hassan.boulahyaoui@um5s.net.ma
Figure 1
Phylogenetic analysis based on the VP4 gene nucleotide sequences of Moroccan P[14] strains, and sequences from the Genbank database. Moroccan strains obtained from vaccinated children are marked with a filled circle. Moroccan strains isolated from goat and bovine are marked with a filled and an empty triangle respectively. The lineages appeared at the right. Numbers at the nodes indicate bootstrap values (values <60% are not shown).
Figure 2
Alignment of the deduced amino acid sequences inside antigenic epitopes of VP8* proteins of Moroccan P[14] stains analyzed and compared with vaccines and field P[14] strains retrieved from GenBank database.