Potential osmotic of MS medium is a new method for Potato virus Y (PVY) elimination using tissue culture technique. Osmotherapy refers to culture meristem – tip and stem nodes at MS medium saturated with sucrose. Stem nodes of potato were successfully stored for up to 12 month at osmo-preservation medium supplemented with sucrose 50, 60 and 70 gl -1 at 210C. The survival percentage was recorded 85.1, 81.2 and 50.8 for 50, 60 and70 gl -1 respectively for 12 months at 210C. All survival plantlets revealed viability and resumed growth on fresh medium within three weeks. PVY was eliminated from infected potato plants using osmotic potential (Osmotherapy) applied 50 , 60 and 70 gl -1 sucrose at 210C with 73 , 89 and 92 % respectively . In addition to production of microubers in vitro under osmotic potential 8.8 (50 gl-1); 8.2 (60 gl-1) and 6.2 (70 gl -1) number per 5 plantlets at 170C . All PVY results were confirmed with DAS – ELISA.